JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 33
·25976-25989
·
IF 6.7
Luo Bo,Hu Yihan,Zhao Yi,Tao Yuchen,Feng Xiaoyu,Li Dan,Wang Yinge,Hu Xinyue,Wu Yanbing
As part of an ongoing effort to develop novel succinate
dehydrogenase
inhibitors (SDHIs), a range of innovative nicotinamide derivatives
featuring stilbene scaffolds were synthesized and assessed for their
antifungal properties. Lead compounds 6g and 6c demonstrated significant antifungal efficacies against Rhizoctonia solani and Sclerotinia sclerotiorum , with EC 50 (half maximal effective
concentration) values of 0.008 and 0.034 μg/mL, respectively,
surpassing Boscalid (EC 50 = 0.573 and 0.455 μg/mL,
respectively) and Fluxapyroxad (EC 50 = 0.026 and 0.037
μg/mL, respectively). Detached leaf assays further confirmed
their excellent protective and curative effects, outperforming Boscalid
and Fluxapyroxad. Further studies revealed that compound 6g operated through a mechanism akin to Boscalid and Fluxapyroxad.
These results indicated that both 6g and 6c showed great potential as novel SDHIs for effectively managing R. solani and S. sclerotiorum , underscoring the necessity for further research to fully explore
their capabilities.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Ning Zhang,Wei Hu,Ruiquan Hou,Pengrui Du,Xiaoran Miao,Ruifei Wang,Hao Wu,Shengnan Li,Youzhi Li,Zhixiang Zhang,Hanhong Xu
The frequent application of pesticides has caused adverse effects on humans and the environment. Stimuli-responsive nanoparticles can enhance the control efficacy of pesticides to pest insects or fungus and reduce impacts on non-target organisms. In the present work, 4-(hydroxymethyl) phenylboronic acid pinacol ester (PBAP) groups were conjugated onto β-cyclodextrin (β-CD) to form a H 2 O 2 -eliminating material (Ox-bCD), and then the nanoparticiles (PYR@OxbCD) loaded pyraclostrobin (PYR) were successfully fabricated by a nanoprecipitation. The prepared PYR@OxbCD had excellent reactive oxygen species (ROS) release dependency, and significantly enhanced fungicidal activity against Rhizoctonia solani in vitro. PYR@OxbCD could accurately release PYR at infected rice plants, providing a better control efficacy than PYR suspension concentrate (SC). The contact angle of PYR@OxbCD on leaves was reduced, and the spreading and antiphotolysis properties were increased, which may lead to an increase of deposition and a lower dissipation rate . Furthermore, the released PYR from PYR@OxbCD enhanced the activity of peroxidase (POD) and catalase (CAT), and up-regulated the expression of defense genes including PAL , PR10, CHI, CAT, GluPOD and AsPOD. PYR@OxbCD exhibited a much weaker effect on microbial abundance in rice cropping systems. The study provides an idea for smart delivery of fungicides to prevent plant pathogens and offers a more comprehensive view about the resistance of R. solani -infected rice triggered by PYR@OxbCD in levels of phenotype, enzymatic activity , gene expression, and microbiome.
✓ 已验证
产品关联
DOI
高影响因子
PHYSIOLOGIA PLANTARUM
·
2023
·Vol. 175
·期 6
·e14087
·
IF 6.4
Qurban Ali,Abdur Rashid Khan,Sheng Tao,Faheem Uddin Rajer,Muhammad Ayaz,Manzoor Ali Abro,Qin Gu,Huijun Wu,Vladislav Kuptsov,Emilia Kolomiets,Xuewen Gao
Rhizoctonia solani and Xanthomonas oryzae pv. oryzae ( Xoo ) are the two major diseases affecting the quality and quantity of rice production. In the current study, volatile organic compounds (VOCs) of Bacillus spp. were used as green biocontrol agents for plant diseases. In in vitro experiments, Bacillus spp. FZB42, NMTD17, and LLTC93-VOCs displayed strong antimicrobial volatile activity with inhibition rates of 76, 66, and 78% for R. solani and 78, 81, and 76% for Xoo , respectively, compared to control. The synthetic VOCs, namely Pentadecane (PDC), Benzaldehyde (BDH), 1,2-Benz isothiazol-3(2H)-one (1,2-BIT), and mixture (MIX) of VOCs showed high volatile activity with inhibition rates of 86, 86, 89, and 92% against R. solani and 81, 81, 82, and 86%, respectively, against Xoo as compared to control. In addition, the scanning and transmission electron microscopes (SEM and TEM) analyses were performed to examine the effect of Bacillus and synthetic VOC treatments on R. solani and Xoo morphology. The analysis revealed the deformed and irregularized morphology of R. solani mycelia and Xoo cells after VOC treatments. The microscopic analysis showed that the rapid inhibition was due to severe oxidative productions inside the R. solani mycelia and Xoo cells. By using molecular docking, it was determined that the synthetic VOCs entered the active binding site of trehalase and NADH dehydrogenase proteins, causing R. solani and Xoo cells to die prematurely and an accumulation of ROS. In the greenhouse experiment, FZB42, NMTD17, and LLTC93-VOCs significantly reduced the lesions of R. solani 8, 7, and 6 cm, and Xoo 7, 6, and 6 cm, respectively, then control. The synthetic VOCs demonstrated that the PDC, BDH, 1,2-BIT, and MIX-VOCs significantly reduced R. solani lesions on leaves 6, 6, 6, and 5 cm and Xoo 6, 5, 5, and 4 cm, respectively, as compared to control. Furthermore, plant defence-related genes and antioxidant enzymes were upregulated in rice plants. These findings provide novel mechanisms by which Bacillus antimicrobial VOCs control plant diseases.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Bo Luo,Yuerui Wu,Xinran Ren,Huimin Li,Xuanru Li,Gege Wang,Mengjia Wang,Luqi Dong,Mengxing Liu,Wei Zhou,Lailiang Qu
In the search for novel succinate dehydrogenase inhibitor (SDHI) fungicides to control Rhizoctonia solani, thirty-five novel pyrazole-4-carboxamides bearing either an oxime ether or an oxime ester group were designed and prepared based on the strategy of molecular hybridization, and their antifungal activities against five plant pathogenic fungi were also investigated. The results indicated that the majority of the compounds containing oxime ether demonstrated outstanding in vitro antifungal activity against R. solani, and some compounds also displayed pronounced antifungal activities against Sclerotinia sclerotiorum and Botrytis cinerea. Particularly, compound 5e exhibited the most promising antifungal activity against R. solani with an EC50 value of 0.039 μg/mL, which was about 20-fold better than that of boscalid (EC50 = 0.799 μg/mL) and 4-fold more potent than fluxapyroxad (EC50 = 0.131 μg/mL). Moreover, the results of the detached leaf assay showed that compound 5e could suppress the growth of R. solani in rice leaves with significant protective efficacies (86.8%) at 100 μg/mL, superior to boscalid (68.1%) and fluxapyroxad (80.6%), indicating promising application prospects. In addition, the succinate dehydrogenase (SDH) enzymatic inhibition assay revealed that compound 5e generated remarkable SDH inhibition (IC50 = 2.04 μM), which was obviously more potent than those of boscalid (IC50 = 7.92 μM) and fluxapyroxad (IC50 = 6.15 μM). Furthermore, SEM analysis showed that compound 5e caused a remarkable disruption to the characteristic structure and morphology of R. solani hyphae, resulting in significant damage. The molecular docking analysis demonstrated that compound 5e could fit into the identical binding pocket of SDH through hydrogen bond interactions as well as fluxapyroxad, indicating that they had a similar antifungal mechanism. The density functional theory and electrostatic potential calculations provided useful information regarding electron distribution and electron transfer, which contributed to understanding the structural features and antifungal mechanism of the lead compound. These findings suggested that compound 5e could be a promising candidate for SDHI fungicides to control R. solani, warranting further investigation.
✓ 已验证
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2026
·
IF 4.8
Xiaoyi Pan,Hongyan Si,Suhua Yuan,Ruyin Li,Guoxiang Liao,Wuxiong Wang,Ji Zhang,Jie Song,Shangxing Chen,Shengliang Liao,Zongde Wang
Rhizoctonia solani is a significant soil-borne plant pathogenic fungus. Its sclerotial structure confers strong environmental adaptability and persistent infectivity, posing a serious threat to the production of numerous crops. Citral, a plant-derived monoterpene aldehyde, exhibits antifungal activity; however, its antifungal potency is limited. In this study, citral was combined with its acylthiourea derivatives, leading to the screening and construction of an efficient synergistic antifungal system, CE4. CE4 significantly inhibits mycelial growth of R. solani and reduces its capacity for sclerotia formation, with an EC 50 value of 10.97 mg/L. Further research has revealed that CE4 disrupts the surface structure of hyphae, significantly increases cell membrane permeability, and reduces ergosterol content, thereby disrupting cellular membrane homeostasis. Meanwhile, CE4 induces the accumulation of ROS and lipid peroxidation products, significantly disrupts the antioxidant enzyme system, and inhibits the activity of key enzymes in energy metabolism, thereby reducing ATP levels. Cytotoxicity evaluation demonstrated that CE4 did not significantly increase cytotoxicity toward mammalian cells at the effective antifungal concentration. The above results indicate that CE4 effectively inhibits the growth of R. solani through the synergistic disruption of cell membrane homeostasis, induction of oxidative stress, and interference with energy metabolism. The results of this study provide a novel theoretical and experimental foundation for the synergistic application of plant-derived antifungal agents.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 33
·26031-26042
·
IF 6.7
Luo Bo,Wu Yanbing,Zhang Qingsi,Liu Xinyi,Cui Huanxi,Gao Zhanghan,Niu Wenjing,Zhao Xiaoyang,Li Dan,Feng Xiaoyu,Hu Yihan,Chai Jianqi,Fang Kun,Zhu Dongyang
In this study, 30 tetrahydroquinoline-based pyrazole-4-carboxamide
derivatives were designed, synthesized, and screened for antifungal
activities. Among them, compound 6m displayed the strongest
inhibitory activity against Rhizoctonia solani , with a half-maximal effective concentration (EC 50 ) as
low as 0.03 μg/mL, superior to Boscalid (EC 50 = 0.40
μg/mL) and Fluxapyroxad (EC 50 = 0.06 μg/mL).
Further detached-leaf assays on rice verified that 6m could markedly suppress the growth of R. solani with strong protective and curative effects even at 50 μg/mL.
Follow-up mechanistic studies indicated that compound 6m might exert antifungal activity through SDH inhibition-related mechanisms.
These findings suggested that compound 6m might be a
potent candidate as a succinate dehydrogenase inhibitor (SDHI) against R. solani . Further research, including field trials
and biosafety assessment, was needed to realize the potential of compound 6m as an effective tool for rice protection.
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 6
·1667
·
IF 4.412
Jian Wang,Xianfeng Hu,Chenglong Yang,Xiaomao Wu,Rongyu Li,Ming Li
Plant diseases reduce crop yield and quality, hampering the development of agriculture. Fungicides, which restrict chemical synthesis in fungi, are the strongest controls for plant diseases. However, the harmful effects on the environment due to continued and uncontrolled utilization of fungicides have become a major challenge in recent years. Plant-sourced fungicides are a class of plant antibacterial substances or compounds that induce plant defenses. They can kill or inhibit the growth of target pathogens efficiently with no or low toxicity, they degrade readily, and do not prompt development of resistance, which has led to their widespread use. In this study, the growth inhibition effect of 24 plant-sourced ethanol extracts on rice sprigs was studied. Ethanol extract of gallnuts and cloves inhibited the growth of bacteria by up to 100%. Indoor toxicity measurement results showed that the gallnut and glove constituents inhibition reached 39.23 μg/mL and 18.82 μg/mL, respectively. Extract treated rice sprigs were dry and wrinkled. Gallnut caused intracellular swelling and breakage of mitochondria, disintegration of nuclei, aggregation of protoplasts, and complete degradation of organelles in hyphae and aggregation of cellular contents. Protection ofRhizoctonia solaniviability reached 46.8% for gallnut and 37.88% for clove in water emulsions of 1000 μg/mL gallnut and clove in the presence of 0.1% Tween 80. The protection by gallnut was significantly stronger than that of clove. The data could inform the choice of plant-sourced fungicides for the comprehensive treatment of rice sprig disease. The studied extract effectively protected rice sprigs and could be a suitable alternative to commercially available chemical fungicides. Further optimized field trials are needed to effectively sterilize rice paddies.Keywords:Rhus chinensisMill;Syzygium aromaticum;rice sheath blight;antifungal activity
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Zhang Shujing,Liu Qi,Han Yunfei,Han Jinghua,Yan Zhiqiang,Wang Yonghong,Zhang Xing
This study was conducted to purify and identify metabolites of antimicrobial activity against phytopathogens from Xenorhabdus nematophila YL001. Three dipeptide compounds were purified from its cell-free cultural broth and identified as (±)-nematophin, cyclo (L-Pro-Gly), and N, N′-dimethyl-cyclo (L-Phe-L-Leu). Nematophin demonstrated a wider antifungal spectrum than the other two compounds. It also exhibited strong inhibitory effects on mycelial growth of Rhizoctonia solani and Phytophthora infestans with EC50 values of 40.00 and 51.25 μg/ml, respectively. Its (S)-configuration structure [(+)-nematophin] was also synthesized and exhibited higher antimicrobial activity than the enantiomeric mixture. The detached leaf assay revealed that nematophin possessed significant preventive and curative efficacy against R. solani on broad bean leaves showing corresponding control efficacies of 93.01 and 94.93% at 1,000 μg/ml, comparable to those of a chemical fungicide (carbendazim) at 500 μg/ml. Additionally, the pot experiments indicated that nematophin could effectively inhibit the disease extension on rice and broad bean plants caused by R. solani. Nematophin also exerted some adverse influences on the sclerotial development of R. solani by dramatically suppressing their formation and maturation at 40.00 μg/ml, as well as their germination at 15.00 μg/ml. Morphological and ultrastructural observations showed that the hyphae of R. solani became twisted, shriveled, and deformed at the growing points after exposure to nematophin at 40.00 μg/ml, and that the subcellular fractions also became abnormal concurrently, especially the mitochondrial structure. These results indicate that nematophin has great potential to be used as a bio-pesticide in agricultural production.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Xu Renle,Chai Pengfan,Cheng Lei,Sun Yongde,Miao Tongzhou,Niu Quanrui,Gao Yanqing,Li Jian
Based on the excellent fungicidal activity of the rosin-based
Schiff
derivative, compound 3c (Co. 3c), against Rhizoctonia solani , this study further investigated its action
mechanism via multiomics, computer-aided drug design, phenotype, and
physiological indicators. Transcriptomic and proteomic analyses identified
2010 differentially expressed genes (DEGs) and 311 differentially
expressed proteins (DEPs), respectively. Steroid biosynthesis was
the most significantly enriched pathway, and the DEGs and DEPs involved
in sterol biosynthesis were simultaneously upregulated. DFT calculations
revealed that Co. 3c exhibited high chemical reactivity, enabling
stable noncovalent interactions with biomacromolecules. Mycelial phenotypic
observations and physiological measurements confirmed that Co. 3c
disrupted mycelial cellular structures, induced lipid peroxidation,
and reduced the intracellular ergosterol content by 45.0%. Molecular
docking suggested that Co. 3c bound to the active pockets of C-8 sterol
isomerase and sterol 22-desaturase through hydrogen bonds and hydrophobic
interactions. Collectively, these findings provide a theoretical basis
for the development of rosin-derived fungicides.
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Liu Jiayue,Feng Shang,Liu Tingting,Mao Yanan,Shen Shen,Liu Yuwei,Hao Zhimin,Li Zhiyong
The gene family of thaumatin-like proteins (TLPs) plays a crucial role in the adaptation of organisms to environmental stresses. In recent years, fungal secreted proteins (SP) with inducing disease resistance activity in plants have emerged as important elicitors in the control of fungal diseases. Identifying SPs with inducing disease resistance activity and studying their mechanisms are crucial for controlling sheath blight. In the present study, 10 proteins containing the thaumatin-like domain were identified in strain AG4-JY of Rhizoctonia solani and eight of the 10 proteins had signal peptides. Analysis of the TLP genes of the 10 different anastomosis groups (AGs) showed that the evolutionary relationship of the TLP gene was consistent with that between different AGs of R. solani. Furthermore, it was found that RsTLP3, RsTLP9 and RsTLP10 were regarded as secreted proteins for their signaling peptides exhibited secretory activity. Prokaryotic expression and enzyme activity analysis revealed that the three secreted proteins possess glycoside hydrolase activity, suggesting they belong to the TLP family. Additionally, spraying the crude enzyme solution of the three TLP proteins could enhance maize resistance to sheath blight. Further analysis showed that genes associated with the salicylic acid and ethylene pathways were up-regulated following RsTLP3 application. The results indicated that RsTLP3 had a good application prospect in biological control.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Li Zhang,Yizhong Huang,Xinying Duan,Hongyan Si,Hai Luo,Shangxing Chen,Liheng Liu,Haohua He,Zongde Wang,Shengliang Liao
Rice sheath blight, caused by the fungus Rhizoctonia solani, poses a significant threat to rice cultivation globally. This study aimed to investigate the potential mechanisms of action of camphor derivatives against R. solani. Compound 4o exhibited superior fungicidal activities in vitro (EC50 = 6.16 mg/L), and in vivo curative effects (77.5%) at 500 mg/L were significantly (P < 0.01) higher than the positive control validamycin·bacillus (66.1%). Additionally, compound 4o exhibited low cytotoxicity and acute oral toxicity for adult worker honeybees of Apis mellifera L. Mechanistically, compound 4o disrupted mycelial morphology and microstructure, increased cell membrane permeability, and inhibited both PDH and SDH enzyme activities. Molecular docking and molecular dynamics analyses indicated a tight interaction of compound 4o with PDH and SDH active sites. In summary, compound 4o exhibited substantial antifungal efficacy against R. solani, serving as a promising lead compound for further optimization of antifungal agents.
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Sustainable Food Systems
·
2023
·
IF 4.7
Mo Feixu,Long Youhua,Li Ming,Ding Yi,Wu Shuai,Li Rongyu
To find a precise, efficient, and safe method to control rice sheath blight disease (caused by Rhizoctonia solani), this study used the natural product magnolol as the fungicide. Based on the characteristics of cell wall-degrading enzymes (CWDEs) released by R. solani during the early stages of infection, a Mag@CMCS@CPSG (magnolol@carboxymethyl chitosan@carboxymethyl cellulose, pectin, sodium alginate, and glycerol) film was constructed to control the release of magnolol in response to CWDEs. The Mag@CMCS@CPSG film increased magnolol toxicity against R. solani, decreased EC50 from 7.33 μg/mL to 4.37 μg/mL, and effectively blocked the invasion of mycelia. Under the stimulation of CWDEs released during R. solani infection, the cumulative release of magnolol by the Mag@CMCS@CPSG film reached 75.62% after 96 h, and the disease index of rice treated with Mag@CMCS@CPSG film significantly decreased. The maximum field control effect of Mag@CMCS@CPSG film on rice sheath blight disease was 72.47%, which was not significantly different from that of the commercial preparation jinggangmycin; however, the holding period was better. The Mag@CMCS@CPSG film reduced the loss of rice yield and quality caused by infection with R. solani and did not cause phytotoxicity or residue. In conclusion, the Mag@CMCS@CPSG film can achieve accurate and green control of rice sheath blight.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）