SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Dongyan Hu,Jiao Guan,Jiaming Luo,Liushuang Zhang,Zhijuan Bao
RGB probe monitors lysosomal pH in real time. • Biocompatible design suits live-cell imaging. • High sensitivity tracks subtle pH fluctuations. • Enables visualization of lysosome-related pathological processes. Lysosomal pH is tightly linked to diverse cellular physiological and pathological processes, and real-time monitoring of its dynamic shifts is critical for uncovering underlying disease mechanisms. Herein, we developed a new lysosome-targeted pH fluorescent probe RGB with facile synthesis, low cytotoxicity, favorable biocompatibility and efficient cell membrane permeability. Its fluorescence emission intensity exhibits a well-defined linear correlation with environmental pH, enabling rapid, sensitive and quantitative detection of subtle pH changes at the single-lysosome level. RGB functions via a pH-triggered spirolactam ring-opening ICT fluorescence turn-on mechanism, with a measured pKa value of 4.12. Confocal fluorescence imaging results verify that RGB can evaluate oxidative stress-induced cellular damage and dynamically visualize autophagy in live tumor cells. As a robust molecular imaging reagent, RGB holds great promise for subcellular pH detection and basic mechanistic exploration of lysosome-associated disorders. The RGB fluorescent probe provides a simple, biocompatible tool for sensitive real-time monitoring of lysosomal pH. It allows visualization of key cellular events and offers valuable insights for biomedical research on lysosome-related processes. Download: Download high-res image (154KB) Download: Download full-size image
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2022
·
IF 6.814
Guojuan Yan,Bo Kong,Jiaqiang Zhao,Huanhuan Ni,Lei Zhan,Chengzhi Huang,Hongyan Zou
The application of nanostructures to design fluorescence resonance energy transfer (FRET) based sensing platforms has been greatly concerned with the demand for sensitive and selective detection of biomolecules. Here, a novel sensitive turn-on fluorescence strategy based on the FRET mechanism has been designed for hyaluronidase (HAase) detection through the modulation of Cu 2-x Se@HA-Rh6G nanoprobe fabricated by self-assembly of rhodamine 6G (Rh6G) together with Cu 2-x Se@HA nanoparticles through electrostatic adsorption. The Cu 2-x Se@HA had extensive localized surface plasma resonance (LSPR) absorption in the wide range of ultraviolet (UV) to near-infrared (NIR) wavelengths and showed good light capture characteristics, which can be acted as good acceptors in the FRET interactions with Rh6G , inducing its efficient fluorescence quenching . In the presence of HAase, the FRET process was disrupted and the fluorescence signal was recovered. In the range of 0.1–10.0 U/mL, the fluorescence recovery of Rh6G showed a good linear relationship with the concentration of HAase, and the detection limit was 0.06 U/mL. The sensing platform has been used for HAase detection in real urine samples and cancer cells imaging.
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高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Yuanyuan Li,Haowen Li,Tiancong Wang,Gang Liu,Gang Wang,Miaomiao Liu,Li Pan,Juan He,Kai Li
Rhodamine 6G (Rh6G) is a classic dye that has been banned as a food additive due to its harm to human bodies. The illegal addition of Rh6G in foods should be strictly monitored. In this study, a surface-molecularly imprinted fluorescence sensor (TPP-SMIPs) for Rh6G detection was prepared on SiO 2 using an aggregation-induced emission molecule as a fluorescence signal part. TPP-SMIPs exhibited ratiometric response to Rh6G. Favorable linearity between the fluorescence intensity ratios and Rh6G was obtained in the range of 0–10.0 μmol/L. Better site accessibility and higher mass transfer rate were achieved in TPP-SMIPs than in non-surface imprinted polymers. High sensitivity with a low detection limit (2.82 nmol/L) and excellent selectivity were also realized in TPP-SMIPs. Its applications in real sample detection showed satisfactory recoveries and relative standard deviations. This study demonstrated that this sensor can provide fast, sensitive and selective determination to Rh6G in real samples. This work not only reported a new detection method for rhodamine dyes, but also provided a universal strategy for the design of high-performance molecularly imprinted fluorescence sensors.
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OPTICAL MATERIALS
·
2025
·
IF 4.2
Yanfen Wang,Pengfei Jia,Jie Ai,Gangzheng Liu,Shuangshuang Liu,Juan Gao,Zhaoqi Sun
Novel Ag/ZnO composite nanoflowers (CNFs) had been designed as a surface-enhanced Raman scattering (SERS) substrate for organic molecule detection. Flower-like ZnO architecture was firstly synthesized by a low-temperature precipitation method, and subsequently Ag nanoparticles were decorated on ZnO nanoflowers through a photochemical reaction. The optimal Ag component was investigated by changing the photo-reduction time of Ag + in the ZnO precursor solution. It is found that the Ag nanoparticles with suitable size and crystallinity are significantly related to the light absorption and Raman signals for Ag/ZnO substrate. For Rhodamine 6G (R6G) as probe molecules, the optimal Ag/ZnO-40 min CNFs achieve a low detection limit of 1.0 × 10 −12 M and a high enhanced factor (EF) of 1.74 × 10 6 . Due to its excellent photocatalytic activity, the Ag/ZnO active substrate effectively degrades R6G molecules adsorbed on the surface, displaying an excellent self-cleaning property and stable recyclability. Such a superior SERS performance can be attributed to the synergy effect of two components (Ag nanoparticles and ZnO nanoflowers) such as its unique architecture, charge transfer and high-density hot spots.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yuanyuan Liu,Lili Yu,Zhuxin She,Ling Li,Tailong Ji,Yi Li,Yuqiao Wang
Four rhodamine 6G-PAH probes with pyrene ( R6G-Pyr ), anthracene ( R6G-Ant ), acenaphthene ( R6G-Acp ) or phenanthrene ( R6G-PA ) as fluorophore were designed and synthesized for Hg(II) detection. Probe R6G-PA , which had the lowest detection limit of 0.84 nmol/L, displayed the best fluorescence performance as compared to the other three probes. This type of probe had good anti-interference properties against most common metal ions except Cu(II). Metal Cu(II) had a certain quenching effect on the fluorescence generated by Hg(II), with a minimum detection limit of 0.31 nmol/L (for R6G-Acp ), indicating its potential practicability for Cu(II) detection. The structure-fluorescence relationship was discussed based on density functional theory (DFT) calculations, and R6G-PA + Hg(II), which had the minimum dihedral angle between polycyclic aromatic rings and rhodamine spiro ring, produced the strongest π-π accumulation and provided the brightest fluorescence. Probe R6G-PA was successfully employed for fluorescence detection of Hg(II) in biological samples. Its solid-phase sensor PS@R6G-PA was developed by immobilizing R6G-PA on PS microspheres for the determination of Hg(II) in water and food samples, with excellent reproducibility and fluorescence “on/off” response. The relative error of the spiked recovery rate was less than 10 %.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Jun Wan,Kun Zhang,Chao Li,Yuyuan Li,Shuyan Niu
A new selective fluorescent chemosensor based on rhodamine 6G derivative ( R1 ) has been designed and synthesized, which displayed highly selective and sensitive detecting for lead ion in aqueous solution. Sensor R1 was selective toward Pb 2+ ion by a light yellow to pink change for the visual detection of Pb 2+ ion. The fluorescence recognition processes of Pb 2+ was based on a binding induced ring-opening from the spirolactam (nonfluorescent) to acyclic xanthenes (fluorescent). Sensor R1 was excited with a 1:1 binding ratio and the linear response in the range of 1.0 × 10 −8 –1.0 × 10 −5 mol L −1 with the limit of detection of 2.7 × 10 −9 mol L −1 (3σ/slope).
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JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Wang Lubiao,Zhang Weiwei,Wu Linfang,Li Feng,Wang Mengyu
Although Rhodamine 6G (R6G) has previously been considered insensitive to pH and stress, with no established applications in sensing these specific parameters, this article presents a breakthrough by re-evaluating its potential as active materials for temperature, pH, and stress sensing. This breakthrough is achieved by employing “balance center” instead of peak maximum or mean wavelength for characterizing the spectral emission band. To verify the improvement, R6G/resin composite samples were prepared. Then the balance center among all the spectral characteristics ensured the most precise identification of the fluorescence band shifts caused by any environmental changes onto the samples. Sensitivities of the prototype sensors with balance center output were calibrated as 0.0653(3) nm/°C in the range of 20 ~ 60 °C, 0.339(8) nm/pH in the pH range of 5.71 ~ 9.44, and − 0.63(2) nm/MPa (equivalently 18 cm -1 /MPa) in the stress range of 0.07 ~ 1.43 MPa, respectively. Corresponding resolutions of the sensing systems were determined as 0.15 °C, 0.18 pH, and 0.03 MPa. These example sensors can be utilized in cytobiology research, demonstrating the potential for extensive sensing applications with a wide range of other fluorescent dyes.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Lu Li,Xinyan Chen,Yuxuan Zhang,Luqiao Tian,Chaoran Lei,Miao Lin,Juan Wei,Dejian Huang,Yongquan Xu,Guiqiang Fei
A Rhodamine 6G fluorescence sensor was developed through synergistic enhancement combining β-cyclodextrin host–guest regulation with hollow-sphere photonic crystals amplification. • The sensor enabled ultrasensitive detection of (-)-epigallocatechin gallate with an ultra-low theoretical detection limit of 5.1 × 10 -16 mol·L -1 and a broad concentration-dependent fluorescence response • The sensing platform exhibited excellent reproducibility and strong anti-interference capability, demonstrating reliable performance in complex sample matrices. (−)-Epigallocatechin gallate (EGCG) is one of the most abundant bioactive polyphenols in tea, and its rapid and sensitive determination is of great importance for food quality control and safety evaluation. Herein, a highly sensitive fluorescence sensing platform based on the synergistic enhancement of hollow-sphere photonic crystals (HSPCs) and β-cyclodextrin (β-CD) is developed for the quantitative detection of EGCG. The photonic band-edge effect and light localization effect of HSPCs, combined with microenvironment regulation induced by the host–guest inclusion interaction of β-CD, synergistically amplify the fluorescence emission intensity of rhodamine 6G (Rh6G). Under acidic conditions, EGCG reacts with ammonium molybdate to form molybdate ester complexes, which induce selective fluorescence quenching of Rh6G, enabling an indirect sensing strategy with a good linear response. Benefiting from the synergistic contribution of structural optical field modulation and molecular microenvironment engineering, the proposed sensor achieves a theoretical limit of detection (LOD) of 5.1 × 10 −16 mol·L −1 , together with good selectivity, anti-interference capability, and reusability. In practical tea sample analysis, satisfactory recoveries are obtained, confirming the accuracy and reliability of the method. This study demonstrates that synergistic fluorescence amplification provides an effective strategy for the sensitive detection of bioactive small molecules in complex food matrices. Download: Download high-res image (139KB) Download: Download full-size image
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Hong Yan Zou,Qiang Wang,Tong Yang,Peng Fei Gao,Chun Mei Li,Yuan Fang Li,Cheng Zhi Huang
Synthesis and engineering of nanostructures with multiple components for the acceptable biosensing platform is a hot topic research. Herein, flower-like nonstoichiometric copper chalcogenides nanocrystals (Cu 2− x S y Se 1− y NCs) loaded with rhodamine 6G (R6G) were used as a selective and sensitive off-on fluorescent probe for biothiols based on inner filter effect (IFE). The Cu 2 −x S y Se 1− y showed the strong interband transition band in the UV–vis region and broad localized surface plasmon resonance (LSPR) in the near-infrared region which exhibited good light-harvesting features, and thus can show sufficient absorption of the excitation or emission light of fluorescent R6G with high fluorescence quenching ability. When the biothiols (glutathione, homocysteine and cysteine) were added, the interband transition bands of Cu 2− x S y Se 1− y NCs were suppressed, resulting in the fluorescence of R6G switched on. There was a good linear relationship between fluorescent recovery of R6G and the concentrations of glutathione, homocysteine and cysteine over the range of 1.0–150 μM, 1.0–200 μM, 1.0–150 μM with a detection limit of 32.0 nM, 34.0 nM and 31.0 nM, respectively. The established strategy exhibited the selective response to biothiol compounds and was also been applied for detecting biothiols in the human serum samples.
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高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Muhammad Haroon,Li Wang,Haojie Yu,Raja Summe Ullah,Zain-ul-Abdin,Rizwan Ullah Khan,Qing Chen,Jian Liu
Carboxymethyl starch- g -polyvinylpyrolidones (CMS- g -PVPs) were prepared by grafting of carboxymethyl starch (CMS) with N -vinylpyrolidone (NVP) using different initiators. The grafting percentage of all the modified starches was determined and the modified starch (CMS- g -PVP-5) with maximum grafting percentage was characterized by elemental analysis, 1 H NMR and FT-IR spectroscopy. Crystallinity and thermal properties of CMS- g -PVP-5 were analysed by XRD and TGA, respectively. CMS- g -PVP-5 was then utilized for the adsorption of Rhodamine 6G (dye) from water under different p H, temperature, adsorbate doze and adsorbent concentration. This modified starch showed good adsorption ability towards Rhodamine 6G. CMS- g -PVP-5 was also applied for the adsorption of ammonia gas and proved an exciting adsorbent for ammonia.
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Plasmonics
·
2013
·Vol. 8
·期 2
·1003-1009
·
IF 2.425
Zhu Jian,Gao Hong-wen,Li Jian-jun,Zhao Jun-wu
Carcinoembryonic antigen (CEA) was used as a separator to prevent the Rhodamine 6G (R6G)-induced aggregation of colloidal gold nanoparticles. The destroyed aggregation has been monitored by measuring the absorption and resonance light scattering peaks corresponding to the longitudinal surface plasmon resonance (SPR) of the chain-like aggregated gold nanoparticles (AuNPs). It was found that the pre-adding of CEA with different concentrations to the gold colloids before mixing them with R6G could lead to the longitudinal SPR peak decrease and blue shift. By analysing the intensity changing and wavelength shifting of the absorption spectra, CEA could be detected in a linear range from 0.2 to 4 ng/mL, and the limit of detection reaches to 0.1 ng/mL. The sensitivity of the CEA concentration dependent shifting and quenching of the plasmonic absorption and scattering corresponding to the AuNPs aggregation presents a well potential application of biologic spectral sensing.
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RSC Advances
·
2023
·Vol. 13
·期 15
·10135-10143
·
IF 3.9
Yangjie Chen,Juan Hao,Zhihang Yin,Qinghui Wang,Youting Zhou,Lingpu Jia,Huiming Li,Wenlong Liao,Kunping Liu
Internal standard molecule 4-mercaptobenzoic acid (4-MBA) embedded Au core-Ag shell nanorods (Au-MBA@Ag NRs) were prepared by a seed-mediated growth method, then loaded on octahedral MIL-88B-NH2 to obtain a novel ratiometric SERS substrate of Au-MBA@Ag NRs/PSS/MIL-88B-NH2 (AMAPM) for detecting rhodamine 6G (R6G) in chili powder. The porous structure and excellent adsorption ability of MIL-88B-NH2, allowed for increased loading of Au-MBA@Ag NRs, thereby shortening the distance between adsorbed R6G and the “hot spot” resulting from local surface plasmon resonance (LSPR) of Au-MBA@Ag NRs. Based on the SERS characteristic peak ratio of R6G to 4-MBA, the ratiometric SERS substrate displayed improved accuracy and excellent performance for R6G detection, with a wide linear range of 5–320 nM and a low detection limit of 2.29 nM as well as fine stability, reproducibility and specificity. The proposed ratiometric SERS substrate offered a simple, fast and sensitive sensing strategy for R6G detection in chili powder, which demonstrated potential applications in food safety and the analysis of trace analytes in complex matrices.
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