ChemElectroChem
·
2020
·Vol. 7
·期 20
·4239-4244
·
IF 4.154
Fan Yuan,Mohamed Ibrahim Halawa,Xiangui Ma,Abubakar Abdussalam,Baohua Lou,Guobao Xu
Graphical Shine and detect : Oxamic hydrazide (OHA) can dramatically increase the electrochemiluminescence (ECL) intensity of Ru(bpy) 3 2+ on glassy carbon electrode (GCE). 4-Nitrobenzaldehyde (4-NBA) can react with OHA to form a hydrazone product through nucleophilic addition, inducing a significant quenching on the ECL intensity of the developed Ru(bpy) 3 2+ /OHA system. Based on this, a sensing platform of 4-NBA was constructed. Oxamic hydrazide (OHA) was utilized as an efficient coreactant of Ru(bpy) 3 2+ for the first time, which can dramatically increase the electrochemiluminescence (ECL) intensity of Ru(bpy) 3 2+ on glassy carbon electrode (GCE). 4-nitrobenzaldehyde (4-NBA), a nitroaromatic compound, has mutagenic and carcinogenic toxicity on humans′ genome and causes a serious impact on the environment. Hence, the detection of its level in biological and environmental samples is of great significance. Interestingly, 4-NBA was found to induce a dramatic quenching for the ECL intensity of the developed Ru(bpy) 3 2+ /OHA system owing to the formation of hydrazone product through the nucleophilic addition reaction with OHA. Under optimal conditions, the ECL decreasing value of Ru(bpy) 3 2+ /OHA in the presence of 4-NBA is linear in the range of 20 μM to 800 μM with a limit of detection (LOD) of 1.09 μM (signal-to-noise 3 : 1). Finally, this strategy enabled the detection of 4-NBA in tap water with satisfactory recoveries from 97.84 % to 103.56 %.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Pei Zhang,Yao Zhang,Xin Xiong,Yao Lu,Nengqin Jia
A sensitive electrochemiluminescence (ECL) immunosensor was successfully fabricated utilizing Ru(bpy) 3 2+ -doped mesoporous polydopamine (MPDA) for subtle exploration of the diabetes biomarker glycosylated hemoglobin (HbA1c). In this protocol, with the advantages of relatively large specific surface area and good biocompatibility, MPDA nanoparticles are capable of loading more Ru(bpy) 3 2+ to greatly amplify the ECL efficiency during the existence of trinpropylamine (TPA) as common coreactant. The acquired Ru(bpy) 3 2+ @MPDA nanoparticles are used as a beneficial substrate for immobilizing aptamer, thereby specifically recognizing the targeting substance HbA1c as much as possible with high selectivity. It could be noted from the experimental results that the ECL intensity was evidently reduced as the proposed Ru(bpy) 3 2+ @MPDA ECL immunosensor captured HbA1c, which showed the principle of the ECL quenching determination. Under optimal experimental conditions, the prepared Ru(bpy) 3 2+ @MPDA ECL immunosensor exhibited wide linear range from 0.1% to 18.5% with the low detection limit (LOD) of 0.015% (S/N = 3) and the correlation coefficient (R 2 ) of 0.994. Moreover, the Ru(bpy) 3 2+ @MPDA immunosensor displayed high sensitivity and selectivity for detecting HbA1c. Therefore, the as-obtained Ru(bpy) 3 2+ @MPDA ECL immunosensor will open up a new promising platform for highly sensitive bioassay of HbA1c.
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高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Jiali Ju,Qiaoyu Ding,Jianwei Xie,Guixin Li
A graphene oxide-Ru(bpy)32+-polyaniline-nanocomposite (GO-Ru(bpy)32+-PANI) was prepared to develop a sensitive label-free electrochemiluminescence (ECL) immunosensor for mucoprotein 1 (MUC1). Ru(bpy)32+ and PANI were decorated together on graphene oxide for the first time. The GO-Ru(bpy)32+-PANI material was characterized by scanning electron microscopy (SEM), absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), fluorescence, electrochemical impedance spectroscopy (EIS), and ECL. The GO-Ru(bpy)32+-PANI exhibited excellent stability. The ECL immunosensor for MUC1 was fabricated by covalently immobilizing anti-MUC1 upon GO-Ru(bpy)32+-PANI modified indium-doped tin oxide (ITO) electrode. In the presence of MUC1, a decrease of ECL was observed due to the formation of the anti-MUC1/MUC1 complex. The immunosensor showed a linear relationship from 1 × 10−13 to 1 × 10−7 mg/mL with a detection limit of 8.2 × 10−14 mg/mL. The immunosensor was applied to the determination of MUC1 in human serum.
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高影响因子
ELECTROCHEMISTRY COMMUNICATIONS
·
2011
·
IF 4.287
Kai Zeng,Manli Guo,Yingjun Zhang,Meng Qing,Afang Liu,Zhou Nie,Yan Huang,Yuliang Pan,Shouzhuo Yao
A novel method based on click chemistry for the immobilization of Ru(bpy) 3 2+ derivative on electrode to fabricate a solid state electrochemiluminescence (ECL) sensor was reported. Pentenyl functionalized Ru(bpy′) 3 Cl 2 was synthesized and immobilized on a (3-mercaptopropyl) trimethoxysilane (MPTMS) pretreated indium tin oxide (ITO) substrate via thiol-ene click chemistry reaction between its alkenyl group and the sulfhydryl group of MPTMS. The immobilization method is simple, fast, and efficient. More importantly, the resulting Ru(bpy′) 3 2+ monolayer film exhibited excellent stability in organic solvent, which is superior to many previously reported methods. Using tri- n -propylamine (TPrA) as a model analyte, the ECL sensor gave a wide linear range over 5 × 10 − 6 to 5 × 10 − 3 M, with a satisfactory detection limit of 1 × 10 − 6 M ( S / N = 3).
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2012
·
IF 3.898
Tingting Hao,Zhiyong Guo,Shuping Du,Lili Shi
An ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA) in serum and saliva was developed. Ru(bpy) 3 2+ -graphene-Nafion composite was first coated on the glassy carbon electrode surface in this ECL immunosensor. The sandwich-type immunoreactions between the first antibody and the second antibody bridged the donors Ru(bpy) 3 2+ and acceptors QDs , which led to the occurrence of ECL quenching of Ru(bpy) 3 2+ by QDs . Based on the efficient ECL quenching, ultrasensitive CEA detection was realized. Under optimal conditions, the ECL signal depended linearly on the logarithm of the CEA concentration within a range from 0.005 to 0.5 pg/mL, and the detection limit of CEA was 0.002 pg/mL. Moreover, the proposed ECL immunosensor provides a stable, specific, and highly sensitive immunoassay protocol for CEA detection at very low levels, which might hold a promise for clinical application.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-11
·
IF 5.3
Tian Li,Li Huiling,Han Pengfei,Ma Guangping,Wang Wenzhuo,Jiang Hanyue,Lu Juan
An electrochemiluminescence (ECL) sensor was constructed based on Ru(bpy) 3 2+ @Au and GO@CdS for the detection of tartrazine (TTZ). In the presence of the co-reactant TPrA, Ru(bpy) 3 2+ and CdS quantum dots (QDs) were used as dual luminophores to synergistically amplify the ECL signal of the sensor. Gold nanoparticles (Au NPs) enhanced the electron transport capability of the system and thereby amplified the ECL intensity. Graphene oxide (GO), as a carrier, has a special wrinkle-like structure that not only adsorbed a substantial amount of CdS QDs but also prevented their agglomeration. Additionally, a more sensitive detection method was established based on the quenching of the ECL signal, which occurred due to the interaction between Ru(bpy) 3 2+ and TTZ. A low detection limit (1.3 × 10 –13 mol·L −1 , S/N = 3) was obtained for TTZ concentrations ranging from 4.0 × 10 –13 to 4.0 × 10 –8 mol·L −1 . Finally, actual measurement results from three beverage samples demonstrated that the prepared sensors possess excellent practical capabilities for TTZ detection. Graphical
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PubMed
高影响因子
ANALYST
·
2021
·Vol. 146
·期 16
·5198-5203
·
IF 4.616
Shimeles Addisu Kitte,Fuad Abduro Bushira,Haijuan Li,Yongdong Jin
Thioacetamide (TAA) is a well-known hepatotoxic substance, so it is important to determine its presence and content in food and environmental samples. Herein, we report a highly sensitive determination method for TAA based on the electrochemiluminescence (ECL) of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) for the first time by using TAA as a new coreactant for Ru(bpy)32+ ECL via an anodic route. The developed Ru(bpy)32+–TAA ECL system allows the determination of TAA with a good dynamic linear range and low limit of detection (LOD) of 0.1 μM to 1000 μM and 0.035 μM (3σ/m), respectively. In addition, the established ECL system can be applied to detect TAA in fruit juice and waste water samples with outstanding recoveries.
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Muhammad Saqib,Zhang-Jian Cheng,Si-Yang Lin,Mohib Ullah,Ai-Lin Liu,Yun Lei
Analyte-induced self-enhanced electrochemiluminescence (ECL) is a powerful strategy for simplifying ECL sensing and improving the overall sensitivity without the addition of external coreactants. However, most Ru(bpy) 3 2+ -based ECL systems still rely on external coreactants such as tri- n -propylamine (TPrA) or oxalate, which introduce additional reagents and increase assay complexity. Herein, we report that the moxifloxacin (MFX) can intrinsically function as an efficient coreactant for Ru(bpy) 3 2+ , enabling an external-coreactant-free and self-enhanced ECL system for its sensitive detection. Electrochemical and ECL investigations reveal that MFX participates directly in the ECL pathway through anodic oxidation, generating reactive intermediates that efficiently trigger the excited Ru(bpy) 3 2+ * state. As a result, the Ru(bpy) 3 2+ –MFX system generates ∼17-fold higher ECL intensity than the classical oxalate system and a comparable ECL response to TPrA (∼66%) under identical conditions. Leveraging this intrinsic self-amplification, an ECL method was developed for sensitive and selective detection of MFX. The developed method exhibits two linear dynamic ranges (0.05–200 μM and 200–800 μM) with a limit of detection (LOD, S/N = 3) of 36 nM. The proposed method offers excellent sensitivity, reproducibility, and selectivity against common interferents and ophthalmic excipients. Moreover, validation using commercial ophthalmic formulations demonstrated excellent agreement with HPLC results, supporting the accuracy of the developed method for pharmaceutical quality control. By integrating analyte recognition and signal generation into a single molecular entity, this work opens a new pathway toward simple and green self-enhanced ECL sensing platforms.
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高影响因子
LANGMUIR
·
2025
·
IF 3.9
Maosheng Sang,Yang Han,Heng Zhang,Zhongyu Li,Quanlong Zhou,Xiaoya Meng,Yuhao Zhang,Yemin Guo,Xia Sun,Surayo Rakhmatzhanovna Usmanova,Jamshed Bobokalonov,Wenping Zhao
An “on–off” electrochemiluminescence (ECL) aptasensor was proposed for the highly sensitive and selective detection of ochratoxin A (OTA) in cereals. The substrate nanomaterial employed in the ECL experiments was titanium carbide (Ti3C2Tx) MXene, while cadmium sulfide quantum dots (CdS QDs) were used as coreactants of the ECL reagent tris (2,2′-bipyridyl) ruthenium(II) (Ru(bpy)32+). Compared to tripropylamine, a more toxic conventional coreactant, CdS QDs can react efficiently with Ru(bpy)32+ in the ECL reaction, and the reaction principles of both were also investigated. The effective connection between CdS QDs and Ru(bpy)32+ via the aptamer resulted in a shortened electron transfer distance, which in turn led to an improvement in ECL intensity and the achievement of the first “on” state. The introduction of OTA resulted in the loss of the effective connection between the CdS QDs and Ru(bpy)32+, which caused the sensor to enter an “off” state. The high ECL intensity of the sensor in the “on” state, in contrast with the low ECL intensity of the “off” state, resulted in an increase in the sensitivity and stability of the sensor. The established aptasensor exhibited a low limit of detection (LOD) of 0.03 ng/mL within a linear range of 0.1 ∼ 100 ng/mL, with spiked recoveries ranging from 93.7% to 105.1%, showing good selectivity, stability, and reproducibility for the detection of OTA in cereals.
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PubMed
高影响因子
Journal of Nanomaterials
·
2023
·
IF 3.791
Xiyuan Tong,Fang Fang,Zhuozhe Li,Kun Qian,Yao Xu,Yifeng E
Acetaminophen (AP) is a commonly used drug that has been detected in groundwater systems in many countries, and has received much attention from researchers in recent years due to its potential environmental impact. In this research, uniformly distributed boron nitride quantum dots (BNQDs) were prepared by a simple ultrasound-solvothermal method. Electrochemical luminescence (ECL) spectroscopy confirmed that BNQDs can act as an effective coreactant to create excellent efficiency in amplifying the ECL intensity of ruthenium-based ECL system. Based on the excited state of and the energy transfer quenching of AP oxidation products in the luminescent system, an AP concentration-quenched drug sensor was successfully constructed. For this sensor, a wide linear dynamic range and low detection limits (5.0 × 10−7−1.0 × 10−5 mol/L and 4.8 × 10−9 mol/L, respectively) were achieved. This ECL drug sensor has excellent performance in the accurate determination of AP content, relieving the stress of the previous AP detection process, and has good reproducibility and recovery in actual sample measurements.
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高影响因子
Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 38
·13106-13112
·
IF 6.4
Mingquan Guo,Jiangnan Shu,Dexin Du,Yisha Wang,Hua Cui
Potential resolved electrochemiluminescence (ECL) nanoluminophores are very important for precise analysis and simultaneous determination of multiple targets. Designing ideal potential resolved ECL nanoluminophores, generating multiple high efficiency ECL emissions without overlap and interference of potential and wavelength, is a challenging subject. Herein, we developed a new nanoluminophore, meso-tetra (4-carboxyphenyl) porphine (TCPP)-functionalized chitosan/Ru(bpy)32+/SiO2 (CRuSiO2) (TCPP@CRuSiO2), with cathode and anode ECL emissions by modifying TCPP on the surface of CRuSiO2 nanoluminophores. The TCPP@CRuSiO2 nanoluminophores presented a uniform sphere morphology (∼50 nm). They exhibited two ECL emissions of 695 nm and 610 nm at −1.20 V and 1.15 V, respectively, by using K2S2O8 and tripropylamine (TPrA) as coreactants. Subsequently, the relevant ECL mechanisms are proposed. In the negative potential range, sulfate radicals (SO4˙−) reacted with TCPP−, generated by the electro-reduction of S2O42− and TCPP, to form the excited state TCPP*, resulting in ECL-1. In the positive potential range, Ru(bpy)33+ and TPrA˙, obtained by electro-oxidation of Ru(bpy)32+ and TPrA, reacted with each other to produce the excited state Ru(bpy)32+*, leading to ECL-2. The synthesized TCPP@CRuSiO2 possesses not only potential and wavelength resolved ECL emissions, but also the advantages of easy synthesis, high ECL efficiency, good stability, and easy film formation on the electrode surface. This work enriches ECL nanoluminophores, which are of important application potential in precise analysis and multiplex analysis.
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高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Kaida Kuang,Ya Li,Yu Ji,Yang Liu,Nengqin Jia
In this work, a quenched molecularly imprinting-electrochemiluminescence (MIP-ECL) sensor based on Ru(bpy) 3 2+ @ZnO-Au nanocomposites was constructed to realize the sensitive detection of acrylamide (AM). Ru(bpy) 3 2+ @ZnO-Au nanomaterials show high ECL efficiency. AuNPs has excellent properties and can improve the properties of electrochemiluminescence by promoting the electron transfer process of the luminescent body. The MIP polymer film was formed on the surface of the material and eluted with eluent, leaving an AM imprinting cavity as a specific recognition site. When adding different concentrations of AM, it was specifically recognized by the MIP membrane, and the ECL signal decreased gradually, thus realizing the sensitive detection of AM. Under the optimal conditions, the detection ranges of the MIP-ECL sensor are 1–10 8 nM and the limit of detection (LOD) is 0.123 nM. Therefore, this sensor proposed in this work opens up a new way for the sensitive detection of AM in food.
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高影响因子
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