ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Daifang Wang,Longhua Guo,Rong Huang,Bin Qiu,Zhenyu Lin,Guonan Chen
Electrochemiluminescence (ECL) of Ru(bpy) 3 2+ has been widely used in analytical chemistry . Herein, we present a new approach to enhance the ECL of Ru(bpy) 3 2+ by the localized surface plasmon resonance (LSPR) of gold nanorods (AuNR). Our investigations reveal that the ECL intensity could be greatly enhanced by controlling the distance between Ru(bpy) 3 2+ and the surface of AuNRs. We call this kind of surface plasmon induced ECL enhancement as surface-enhanced electrochemiluminescence (SEECL). This kind of SEECL phenomenon is utilized to fabricate a biosensor for ultrasensitive Hg 2+ detection. The SEECL biosensor is fabricated by self-assembling AuNRs and T-rich ssDNA probes on gold electrode surface. With the presence of Hg 2+ , the conformation of ssDNA probes changed to be hairpin-like structure via formation of T-Hg 2+ -T structure. And Ru(bpy) 3 2+ could insert into the grooves of the hairpin structured DNA probes to generate ECL emission, which could be enhanced by the LSPR of AuNR. The ECL intensity of the sensor increased with the concentration of Hg 2+ , and a detection limit of 10 fM Hg 2+ in aqueous solution was achieved. The effect of different LSPR peak location of AuNR on the sensitivity of biosensor has been investigated. The results show that a good overlap between the LSPR absorption spectrum and the ECL emission spectrum of Ru(bpy) 3 2+ could achieve the best ECL signal enhancement.
研究领域:
分析化学
·
生物传感
·
纳米技术
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Xinyi Ni,Yuan Zhao,Qijun Song
An integrative method based on the combination of electrochemical reduction (ECR) and in-situ electrochemiluminescence (ECL) detection was developed for the sensitive detection of the nitroaromatic compounds (NACs), including 4-nitrotoluene (4-NT), 2,4-dinitrotoluene (2,4-DNT), 2,4,6-trinitrotoluene (TNT). An electrochemical flow cell including two working electrodes was fabricated with poly(dimethylsiloxane) (PDMS) to achieve the dual functions. The first one is a copper mesh electrode, acting as the reductor to convert the NACs to aminoaromatic compounds (AACs). Electrochemical deposition of copper nanoparticles on the surface of the copper mesh was conducted to further increase the reduction efficiency. The second one is an ECL electrode, which was used for in-situ detection of the reduced products. The ECL electrode was fabricated by the immobilization of Ru(bpy) 3 2+ on a glassy carbon electrode (GCE) with the Nafion/the multi-wall carbon nanotubes (MWCNTs) composite film. The copper mesh electrode was arranged directly underneath the ECL electrode to ensure the reduced products could be immediately detected in-situ. An electrolytic solution consists of 33.3 mmol/L Na 2 SO 4 and 16.7 mmol/L phosphate buffer solution (PBS, pH7.0) was found to be optimum for both the electrochemical reduction of the NACs and the ECL detection. The ECL intensities were proportional to the concentrations of 4-NT, 2,4-DNT and TNT in the range from 1.0 × 10 −9 to 1.0 × 10 −5 , 3.5 × 10 −9 to 1.0 × 10 −5 and 7.5 × 10 −9 to 1.0 × 10 −5 mol/L respectively. The limit of detections (LOD) were 4.0 × 10 −10 , 1.7 × 10 −9 and 3.1 × 10 −9 mol/L respectively for 4-NT, 2,4-DNT and TNT. The reduction mechanism was preliminarily investigated and it was found that the presence of oxygen was critical for the reduction process, suggesting that the reductive oxygen species O 2 − , O H could be involved in the NACs reduction process.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Libo Li,Wanlin Zhao,Jiayi Zhang,Lijun Luo,Xiaohong Liu,Xia Li,Tianyan You,Chunjiang Zhao
Herein, a label-free, self-enhanced electrochemiluminescence (ECL) sensing strategy for divalent mercury (Hg(II)) detection was presented. First, a novel self-enhanced ECL luminophore was prepared by combining the ECL reagent tris(2, 2′-bipyridyl) dichlororuthenium(II) hexahydrate (Ru(bpy) 3 2+ ) and its co-reactant carbon nitride quantum dots (CNQDs) via electrostatic interactions. In contrast to traditional ECL systems where the emitter and its co-reactant underwent an intermolecular reaction, the self-enhanced ECL system exhibited a shortened electron-transfer distance and enhanced luminous efficiency because the electrons transferred from CNQDs to oxidized Ru(bpy) 3 2+ via an intramolecular pathway. Furthermore, the as-prepared self-enhanced ECL material was encapsulated in silica (SiO 2 ) nanoparticles to generate a [email protected] 2 luminophore. Based on the different affinity of [email protected] 2 nanoparticles for single-stranded DNA (ssDNA) and Hg(II)-triggered double-stranded DNA (dsDNA), a label-free ECL biosensor for Hg(II) detection was developed as follows: in the absence of Hg(II), ssDNA was adsorbed on [email protected] 2 surface via hydrogen bond, electrostatic, and hydrophobic interaction. Thus, quenched ECL signal was observed. On the contrary, in the presence of Hg(II), stable dsDNA was formed and carried the ssDNA separating from [email protected] 2 surface, resulting in most of [email protected] 2 existing in their free state. Therefore, a recovered ECL intensity was obtained. On this basis, Hg(II) was measured by the proposed method in the range of 0.1 nM-10 μM, with a detection limit of 33 pM. Finally, Hg(II) spiked in water samples was measured to evaluate the practicality of the fabricated biosensor.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Amin Zhang,Chongchong Miao,Hongyuan Shi,Hongkun Xiang,Chusen Huang,Nengqin Jia
Recent years have witnessed the great development of electrochemiluminescence (ECL) as a platform for the analytical processes in many fields, which includes pharmaceutical analysis, immunoassay, DNA analysis as well as clinical analysis. Herein, a novel electrochemiluminescence (ECL) sensor was fabricated for ultrasensitive detection of atropine based on Ru(bpy) 3 2+ /carbon nanospheres/Nafion composite film. The carbon nanospheres (CNPs) in composite film were obtained through a hydrothermal method. Then, electrochemical and ECL behaviors of the proposed sensor were investigated. Under the optimal conditions, the constructed atropine sensor showed a wide linear range from 0.5 to 250 μM with a detection limit of 0.17 μM (at signal-to-noise ratio of 3). Moreover, the results indicated that the CNPs-based ECL sensor exhibited excellent sensitivity and favorable stability, which might be ascribed to the high surface area and the good electrical conductivity of CNPs. Furthermore, the ECL sensor was applied for the determination of atropine in atropine sulfate injection samples and displayed a desirable result. The proposed ECL sensor could be easily obtained and be promising used for drug assay.
研究领域:
分析化学
·
药学
·
电化学
·
纳米技术
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xiu-hua Wei,Xing Qiao,Jie Fan,Yuan-qiang Hao,Yin-tang Zhang,Yan-li Zhou,Mao-tian Xu
Herein, a label-free electrochemiluminescence (ECL) biosensor for sensitive determination of carcinoembryonic antigen (CEA) was fabricated based on CdS quantum dots loaded onto metal organic framework (CdS QDs@MOF) and triethanolamine modified on gold nanoparticles (TEOA@AuNPs) as bi-coreactants of Ru(bpy) 3 2+ ECL system. The TEOA@AuNPs served as not only coreactant of Ru(bpy) 3 2+ but also nanocarrier for CEA aptamer which was bonded to the TEOA@AuNPs through Au–S bond. After incubated in CEA solution, the ECL signals decreased due to the poor conductivity of CEA and there was a linear correlation between the ECL intensity and log(C CEA ). Based on this result, a label-free ECL biosensor has been developed successfully and used to detect CEA with a detection limit of 8.5 × 10 −5 ng/mL. The proposed ECL aptasensor had good analytical performance in the detection of CEA in human serum samples.
研究领域:
生物传感
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医学检测
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纳米技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
He Zhou,Huan Yue,Yali Zhou,Lin Wang,Zhifeng Fu
Novel amorphous carbon nanoparticles (ACNPs) with high electrochemiluminescence (ECL) quenching efficiency were prepared from candle soot with a quite simple approach. The prepared ACNPs were employed as novel energy quenchers for an ECL immunosensor on a disposable screen-printed carbon electrode array (SPCEA) substrate. Gold nanoparticles-doped chitosan composite film was utilized to immobilize Ru(bpy) 3 2+ -labeled antibody on the SPCEA, and ACNPs were linked with antigens to obtain ACNP-antigen conjugate. The immunoreaction between the Ru(bpy) 3 2+ -labeled antibody and the ACNP-antigen conjugate bridged the Ru(bpy) 3 2+ donor and the ACNPs acceptor, which led to quenching of ECL emission from Ru(bpy) 3 2+ via energy transfer. In the presence of the target antigen, this antigen competed with the ACNP-antigen for the immobilized Ru(bpy) 3 2+ -labeled antibody, thus led to decreased occurrence of the ECL quenching. This ACNPs-based ECL quenching system was successfully applied to detect mouse IgG, which was used as a model analyte. The method showed a linear range of 0.50–400 ng mL −1 and a detection limit of 0.35 ng mL −1 . The results for real sample assay and recovery test demonstrated the reliability of this ECL quenching-based immunosensor. It could be further extended to multiplexed immunoassay of biomarker proteins for clinical diagnosis.
研究领域:
分析化学
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生物传感器
·
纳米技术
·
临床诊断
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Hong Ke,Xin Zhang,Weiwei Guo,Amin Zhang,Zhiming Wang,Chusen Huang,Nengqin Jia
Electrochemiluminescent (ECL) assay has gradually drawn increasing interest to be considered as one of the potential strategies to be applicable in a wide range of medicine target determination. In this protocol, a facile and ultrasensitive ECL sensor platform was developed aiming at rapidly quantitative detection of polyphenolic flavonoids based on multiwall carbon nanotubes (MWCNTs)/platinum nanoparticles (PtNPs) nanohybrids/nafion/Ru(bpy) 3 2+ modified glassy carbon electrode (GCE) in Ru(bpy) 3 2+ /tri- n -propylamine (TPA) co-reaction system. Attributed to the synergistic ECL signal amplification of both PtNPs and MWCNTs facilitating the electron transfer, this proposed sensor could sensitively analyze the flavonoids through an annihilation ECL emission mechanism since the less generated amount of excitation state Ru(Ⅱ)* on account of the principle that flavones are prone to donate electrons and scavenge free radicals to further show a competitive relationship with Ru(Ⅲ) on the reaction with TPA*. The influences of pH, Ru(bpy) 3 2+ absorption time and TPA co-reactant concentration were investigated and the sensor exhibited a linear response to flavonoids when the concentration ranges from 6.50×10 –11 to 1.00×10 −7 M with a detection limit (LOD) of 2.17×10 –11 M (S/N=3) upon the optimized conditions. Furthermore, high sensitivity, excellent stability and reproducibility were verified to declare the promise for being applicable to analyze the flavones content in real flavones medicine samples and ginkgo leaves extracts with satisfactory results.
研究领域:
分析化学
·
纳米技术
·
药物分析
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xinyang Zhu,Huanhuan Xing,Yuan Xue,Jing Li,Erkang Wang,Shaojun Dong
A chemical fixation strategy originating from single-atom-anchoring with metal–organic frameworks as a carrying matrix was proposed for solid-state electrochemiluminescence (ECL). Herein, UiO-67(N) with the exposure of 2,2′-bipyridine (bpy) ligands could coordinate with Ru2+ to form a local structure of [Ru(bpy)3]2+ (Ru-UiO). The influence of the steric effect induced with different Ru sources was discussed. The as-obtained Ru-UiO exhibits high ECL intensity and outstanding stability in the presence of a coreactant at low concentrations. The proposed synthesis strategy may hold great potential for the synthesis of solid-state ECL materials and their further utilization in ECL analysis.
研究领域:
材料科学
·
分析化学
·
电化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Sai Lian,Zhiyong Huang,Zhengzhong Lin,Xi Chen,Munetaka Oyama,Xiaomei Chen
In this work, a novel and highly selective molecularly imprinted sensor for the detection of melamine in milk using electrochemiluminescence (ECL) is reported. The preparation was carried out by modification of a glassy carbon electrode with a composite of silica nanoparticles doped with [Ru(bpy) 3 ] 2+ (RUDS) and molecularly imprinted polymer (MIP). The keys of the sensor were to employ RUDS as an ECL luminophore to prevent [Ru(bpy) 3 ] 2+ leaching from the modified electrode and MIP as a material for selective recognition. Taking advantage of the MIP pre-concentration effect, melamine could be extracted from the solution and detected by the resulting ECL signal enhancement of [Ru(bpy) 3 ] 2+ . Under optimized conditions, the relative ECL signal was proportional to the logarithm of melamine concentration in the range of 1 × 10 −12 –1 × 10 −7 mol L −1 , with a detection limit as low as 5 × 10 −13 mol L −1 . Compared with the RUDS/non-imprinted polymer, the RUDS/MIP modified electrode displayed high recognition ability, good reproducibility, and stability. To verify its reliability, the fabricated ECL sensor was used for determining the concentration of melamine in milk.
研究领域:
分析化学
·
食品安全
·
传感器技术
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产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Yi Xiao,Suhua Chen,Simeng Zhang,Guofang Wang,Haomin Yi,Gui-Zhong Xin,Xiaoping Yang
Straightforward and accurate measurement of medical biomarkers is of essential importance in clinical diagnostics and treatments. However, the major challenge is the diversity in dynamic range of different biomarkers ranging from pg mL −1 to μg mL −1 in various body fluids and tissues among patients. Here, we develop a mesoporous silica (MS)-mediated controllable electrochemiluminescence (ECL) quenching of immunosensor that allows accurate immunoassays with simplicity, sensitivity and tunable sensing range. MS is employed to enhance the sensitivity and tune ECL quenching to broaden the detection range just by altering luminophore (Ru(bpy) 3 2+ ) and coreactant (DBAE) concentration without additional modifications. The immunoassay is followed: homogeneous sandwich immunoreaction, magnetic separation, and ECL quenching detection. As a proof-of-concept, simple and sensitive detection of IgG is achieved ranging from pg mL −1 to μg mL −1 , and applications of the strategy are extended by the combination of ECL immunosensor with commercial ELISA kit. This study will not only be expected to serve as a new avenue for the assay of physiological and clinical implications of immunological biomarkers, but also benefit a wide range of applications that require a tunable detection range and ultrahigh sensitivity.
研究领域:
分析化学
·
生物医学工程
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jiajing Xu,Weiqiang Yang,Jiancong Ni,Qingxiang Wang,Zhenyu Lin
Electrochemiluminescence (ECL) arises from the collisional annihilation between the electro-excited luminophore and co-reactant. Most ECL-based bioassays require extensive free co-reactants that collide with luminophores randomly. Herein, the N,N-dimethylaniline (DMA) group was electro-grafted on the ITO electrode surface as the bottom co-reactant layer, and the vertically ordered mesoporous silica film (VMSF) was prepared as the upper layer to regulate the accessibility of Ru(bpy) 3 2+ . Thus, the dual-layer functionalized VMSF/N-ITO was newly built for the controllable ECL study. Relevant conditions, including the DMA electro-grafting, VMSF thickness, buffer concentration and pH value, were discussed to improve the ECL efficiency. Based on the VMSF/N-ITO platform, a homogeneous ECL bioassay was developed for miRNA-21 detection. The target nucleotide sequence induced the hybridization chain reaction (HCR) of probes H1 and H2 to absorb free Ru(bpy) 3 2+ as much as possible. Residual Ru(bpy) 3 2+ permeated the VMSF nanochannels to collide with the DMA co-reactant in producing an ECL response. There was a linear relationship between ∆ECL intensity and logarithmic concentration of miRNA-21 in the 1 fM ∼ 100 pM range, and the detection limit was 0.25 fM. The developed approach was probes immobilization-free, highly sensitive, and anti-interference, opening a new avenue for ECL-based bioassays.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物传感器
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-10
·
IF 6.408
Wang Xin,Li Longwen,Li Lihua,Bu Tong,Yang Kairong,Xia Junfang,Sun Xinyu,Jiang Hong,Wang Li
A novel ratiometric fluorescence probe was designed for the determination of Al 3+ by self-assembling of NH 2 -MIL-101(Fe) and [Ru(bpy) 3 ] 2+ . Under the excitation wavelength of 360 nm, the NH 2 -MIL-101(Fe)@[Ru(bpy) 3 ] 2+ presented a dual-emitting luminescent property at 440 and 605 nm, respectively. In the presence of Al 3+ , the blue fluorescence of NH 2 -MIL-101(Fe)@[Ru(bpy) 3 ] 2+ at 440 nm was enhanced remarkably, while the red emission at 605 nm was almost not influenced. Therefore, taking the fluorescence at 440 nm as the report signal and 605 nm as the reference signal, quantitative determination was achieved for Al 3+ concentration in the ranges 0.2–25 μM and 25–250 μM. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 73 nM and 244 nM, respectively. The sensing mechanisms were studied by theoretical calculation and optical spectra. The analysis of real food samples confirmed the suitability of the proposed method. More importantly, portable fluorescent test papers were successfully manufactured to provide a strategy for visual, rapid, and on-site detection of Al 3+ . Graphical abstract
研究领域:
分析化学
·
材料科学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）