Chemosensors
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2024
·Vol. 12
·期 10
·214
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IF 3.7
Yun Hui,Weijun Kong,Weiliang Shu,Zhiting Peng,Fengshan Shen,Mingyang Jiang,Zhen Xu,Tianzhun Wu,Wenhua Zhou,Xue-Feng Yu
The measurement of cardiac troponin I (cTnI) is of vital importance for the early diagnosis of acute myocardial infarction. In this study, an enhanced electrochemiluminescent immunoassay for the highly sensitive and precise determination of cTnI was reported. A biomimetic chip with nepenthes peristome surface microstructures to achieve single-layer microbead arrays and integrated microelectrode arrays (MEAs) for ECL detection was microfabricated. Ru@SiO2nanoparticles were prepared as signal amplificators labeling immunomagnetic beads. Dendrimer-encapsulated platinum nanoparticles (Pt DENs) were electrochemically modified on ITO MEAs. The resulting Pt DEN-modified ITO MEAs preserved good optical transparency and exhibited an approximately 20-fold ECL signal amplification compared to that obtained from bare ITO. The method made full use of the biomimetic chip with Pt DENs to develop single-layer immunomagnetic bead arrays with increasingly catalyzed electrochemical oxidation of the [Ru(bpy)3]2+–TPA system. Consequently, a limit of detection calculated as 0.38 pg/mL (S/N = 3) was obtained with excellent selectivity, demonstrating significant potential for the detection of cTnI in clinical diagnostics.
研究领域:
生物医学工程
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分析化学
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纳米技术
·
心脏病学
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Chen Cui,Yunfeng Fan,Yaxuan Chen,Renlong Wei,Jie Lv,Meng Yan,Dechen Jiang,Zhimin Liu
Cyanidin-3- O -glucoside (C3G), a natural antioxidant, plays multiple physiological or pathological roles in maintaining human health; thereby, designing advanced sensors to achieve specific recognition and high-sensitivity detection of C3G is significant. Herein, an imprinted-type electrochemiluminescence (ECL) sensing platform was developed using core-shell Ru@SiO 2 -CMIPs, which were prepared by covalent organic framework (COF)-based molecularly imprinted polymers (CMIPs) embedded in luminescent Ru@SiO 2 cores. The C3G-imprinted COF shell not only helps generate a steady-enhanced ECL signal, but also enables specific recognition of C3G. When C3G is bound to Ru@SiO 2 -CMIPs with abundant imprinted cavities, resonance energy transfer (RET) behavior is triggered, resulting in a quenched ECL response. The constructed Ru@SiO 2 -CMIPs nanoprobes exhibit ultra-high sensitivity, absolute specificity, and an ultra-low detection limit (0.15 pg mL −1 ) for analyzing C3G in food matrices. This study provides a means to construct an efficient and reliable molecular imprinting-based ECL sensor for food analysis.
研究领域:
食品科学
·
分析化学
·
生物传感技术
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Yunyun Huang,Hongju Ren,Huihuang Fang,Dong Ouyang,Chongqi Chen,Yu Luo,Li Lin,Dabiao Wang,Lilong Jiang
Ammonia decomposition for onsite hydrogen production has been regarded as an important reaction which links to efficient hydrogen storage, transport and utilization. However, it still remains challenging to develop efficient catalysts with robust stability for ammonia decomposition. Herein, an integrated strategy was employed to synthesize Ru/SiO 2 @N-CS via wrapping a thin layer of N-doped carbon onto the SiO 2 sphere, following the anchor of Ru nanoparticles (NPs) onto the support. The obtained Ru/SiO 2 @N-CS (Ru loading: 1 wt%) shows a promising performance for ammonia decomposition, reaching 94.5 % at 550 °C with a gas hourly space velocity (GHSV) of 30 000 mL g cat -1 h −1 . The combination of the SiO 2 as the core prevents the degradation of N-doped carbon layers and then enhance the durability of the catalysts, remaining stable after 50 h at evaluated temperatures. Adequate characterizations were used to illustrate the effect of microchemical environment on ammonia decomposition activity of Ru/SiO 2 @N-CS catalyst under different calcination atmosphere and the correlation between structure and performance.
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Lijun Luo,Shuai Ma,Libo Li,Xiaohong Liu,Jiayi Zhang,Xia Li,Dong Liu,Tianyan You
Accurate and early diagnosis of mycotoxin is particularly significant to the food and agricultural product safety. In the present work, a sensitive and effective monitoring method for zearalenone (ZEN) was exploited based on a novel self-enhanced electrochemiluminescence (ECL) aptasensor. The self-enhanced lumonophore was compounded by electrostatically combining amine-functionalized Ru(bpy) 3 2+ -doped silica nanoparticles (NH 2 [email protected] 2 NPs) and nitrogen doped graphene quantum dots (NGQDs) together. Since the emitter and co-reactant simultaneously existed in the same nanoparticle, shortened electron-transfer distance and decreased energy loss was obtained. Therefore, self-enhanced ECL aptasensor based on the novel complex expressed the widest linear range of 10 fg mL −1 –10 ng mL −1 and the lowest detection limit of 1 fg mL −1 for ZEN detection. More importantly, ZEN produced during the mildew process of corn flour was monitored by the developed aptasensor, which exhibited superior determination and potential application in real samples.
研究领域:
食品安全
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Hongding Zhang,Sifei Wu,Hui-Jin Xiao,Hai-Bo Wang,Linxia Fang,Jun-Tao Cao
Ultrasensitive analytical methods are still urgent for the discovery of trace level biomarkers and the early clinical diagnosis of disease. In this work, an ultrasensitive universal sensing platform was constructed by integrating fluorescent assay with chemical-chemical redox cycling signal amplification strategy. Using Ru@SiO 2 nanoparticles wrapped by MnO 2 nanosheets (Ru@SiO 2 @MnO 2 ) as fluorescent probe , the chemical-chemical redox cycling system was conducted upon ascorbic acid (AA) and tris(2-carboxyethyl)phosphine (TCEP) as reductants and MnO 2 nanosheets as oxidant. The MnO 2 nanosheets not only could quench the fluorescence of Ru@SiO 2 nanoparticles to reduce the background, but also could serve as oxidants to react with AA, generating dehydroascorbic acid (DHA). The DHA was reduced by TCEP in turn to form AA that participated in the next cycling of chemical-chemical redox reaction. Thus, the constantly released AA from the chemical-chemical redox cycling system could massively etch MnO 2 nanosheets on Ru@SiO 2 surface, making the fluorescence of Ru@SiO 2 nanoparticles greatly recovered. It was shown that the sensitivity of the fluorescent assay was improved almost 52 times by utilizing the chemical-chemical redox cycling signal amplification strategy. This strategy was further employed to detect DNA methylation with the aid of AA-encapsulated liposomes that were modified with 5 mC antibodies to bind with the methylated DNA captured in 96-well plate. A detection of limit down to 16.2 fM was achieved for the detection of methylated DNA. It's believed that the incorporation of chemical-chemical redox cycling signal amplification strategy into fluorescent sensing paves a new way for ultrasensitive detection of biomarkers.
研究领域:
分析化学
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生物标志物检测
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纳米技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Guifen Jie,Junjun Ge,Xiaoshan Gao,Chunli Li
In this work, we designed a new strategy for ultrasensitive detection of CEA based on efficient electrochemiluminescence (ECL) quenching of Ru(bpy) 3 2+ -doped SiO 2 nanocomposite by ferrocene using target recycling amplification technique. A large number of [email protected] 2 ECL signal probe were firstly assembled on the novel magnetic core−shell Fe 3 O 4 @Au nanoparticles (NPs), then the ferrocene-labeled ECL quenching probe (Fc-probe) was linked to the magnetic NPs. Finally, numerous DNA1 sequences were produced by target CEA-triggered multiple recycling amplification and displaced the Fc-probe on the magnetic NPs, leading to significantly enhanced ECL signal for CEA detection. Because of the designed cascade signal amplification strategy, the newly developed method achieved a wide linear range of 10 fg/mL to 10 ng/mL with a low detection limit of 3.5 fg/mL. Furthermore, taking advantages of the magnetic Fe 3 O 4 @Au NPs for carring abundant signal probes, sensing target and ECL detection, the developed ECL strategy is convenient, rapid and displayed high sensitivity for CEA detection, which has great potential for analyzing the clinical samples in practical disease diagnosis applications.
研究领域:
分析化学
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生物传感器
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纳米技术
·
癌症诊断
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
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2020
·
IF 6.215
Dongmiao Qin,Xiaohua Jiang,Guichun Mo,Jinsu Feng,Biyang Deng
A label-free electrochemiluminescence (ECL) immunosensor is developed to detect alpha-fetoprotein (AFP). Ru (bpy) 3 2+ -doped silica nanoparticles (Ru–SiO 2 ) and gold nanoparticle-modified reduced graphene oxide (rGO@Au) are integrated to form an rGO@Au@Ru–SiO 2 composite. The composite is employed as a carrier for immobilizing the target antibody, and a label-free ECL immunosensor is constructed via antigen–antibody interaction. Owing to the numerous Ru (bpy) 3 2+ molecules in the Ru–SiO 2 , a high ECL signal is acquired. With excellent conductivity and a large surface area, the rGO@Au composite not only accelerates electron transfer but also improved the ECL signal and captures antibodies, thus enhancing ECL response and sensitivity. Moreover, boron nitride quantum dots (BNQDs) are used as rGO@Au@Ru–SiO 2 coreactants, achieving high ECL intensity. The proposed ECL system exhibits significant potential in bioanalysis , which is attributed to the excellent biocompatibility and good stability of BNQDs. Under optimal experimental conditions, the sensor has a linear range of 0.0001 ng mL −1 to 100 ng mL −1 and a limit of detection equal to 0.03 pg mL −1 (S/N = 3). The proposed technique is successfully applied in the susceptible detection of AFP in human serum. The recovery ranges from 99% to 100%, and the RSD is less than 2.8%. The constructed ECL immunosensor exhibits acceptable performance during actual application.
研究领域:
生物分析
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医学诊断
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产品关联
DOI
高影响因子
FUEL
·
2025
·
IF 7.5
Yongyuan Deng,Yichao Ye,Tao Yin,Jinyao Mai,Zhipeng Tian,Chao Wang,Ying Chen,Zhi Yang,Junling Tu,Riyang Shu
Hydrodeoxygenation (HDO) technology is an essential method for efficiently converting phenolic compounds from biomass into high-quality liquid fuels. Rationally designed catalysts not only significantly enhance stability and reaction rates, but also improve product selectivity and fuel quality. In this study, we proposed a Zr 3+ -defect-rich core–shell structured catalyst Ru@SiO 2 -ZrO 2 and employed it for the HDO of phenolic compounds and lignin pyrolysis oil. Ru nanoparticles are uniformly dispersed and have small particle sizes, showing outstanding catalytic hydrogenation activity. The porous SiO 2 shell possesses a high specific surface area with a high thermal stability, facilitating reactant transport and maintaining catalyst structural integrity. ZrO 2 serves as an acid modifier to enhance the catalyst acidity and promote the deoxygenation procedure. Besides, ZrO 2 enhances the adsorption of phenolic compounds and improves the cleavage of C-O bonds, due to its abundant oxygen vacancies . These factors make the catalyst achieved complete conversion of guaiacol to cyclohexane at mild reaction temperature of 240°C, which is superior to other similar reported results. Compared to conventional supported Ru/SiO 2 -ZrO 2 catalysts, the unique core–shell structure of Ru@SiO 2 -ZrO 2 obtained a sustained catalytic activity after five consecutive recycles without significant deactivation. Moreover, this core–shell catalyst exhibited good HDO performance for various phenolic compounds and lignin pyrolysis oil. The hydrocarbon content increased significantly from 12.7 % to 96.9 % in the upgrading of lignin pyrolysis oil, demonstrating the catalyst’s great potential for future industrial applications.
研究领域:
催化化学
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生物质能源
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材料化学
·
绿色化工
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能源化学工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Lifang An,Jie Fan,Buhuan Wang,Jingyi Yang,Guolian Wang,Meng Zhang,Zhongyi Liu
Ru/Fumed SiO 2 achieves 100% DMT conversion and 100% DMCD selectivity. • Macropores of Fumed SiO 2 lower diffusion barriers and enhance active site utilization. • Strong Ru-O-Si interaction promotes Ru dispersion and ensures 5-cycle reusability. • Low apparent activation energy enables high-efficiency aromatic ring hydrogenation. The growing accumulation of waste polyethylene terephthalate (PET) causes severe resource depletion and environmental pollution, making its high-value chemical recycling essential for sustainable development. PET methanolysis affords dimethyl terephthalate (DMT), and its selective hydrogenation to dimethyl 1,4-cyclohexanedicarboxylate (DMCD) connects waste PET valorization with high-performance polyester synthesis. Herein, a series of Ru-based catalysts supported on microporous-dominated S-1, ordered mesoporous MCM-41 and SBA-15, and Fumed SiO 2 with the open interparticle macro-mesoporous were prepared by the incipient wetness impregnation, and the role of support pore structure in DMT hydrogenation to DMCD was systematically examined. The results show that the open interparticle macro-mesoporous framework of Fumed SiO 2 alleviates diffusion constraints and improves the accessibility of Ru active sites. This structure enables uniform Ru dispersion, giving Ru/Fumed SiO 2 the smallest average Ru particle size (6.0 nm). Surface Si-OH groups anchor Ru species through Ru-O-Si interfacial structures, which strengthens metal-support interactions and promotes H 2 activation and active-site utilization. Under 180 °C, 3.0 MPa H 2 , and 3 h, Ru/Fumed SiO 2 delivers 100% DMT conversion and 100% DMCD selectivity. It also shows the lowest apparent activation energy (39.0 kJ·mol −1 ), stable performance over five cycles, and over 93% conversion and selectivity for representative substituted aromatic compounds. These findings demonstrate that an open interparticle macro-mesoporous framework could improve molecular accessibility, mitigate mass-transfer limitations, and enhance hydrogenation efficiency. Download: Download high-res image (185KB) Download: Download full-size image
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DOI
高影响因子
FUEL
·
2020
·
IF 5.578
Riyang Shu,Rongxuan Li,Biqin Lin,Bowen Luo,Zhipeng Tian
Metal particle size and metal dispersion significantly influence the catalyst activity, and an efficient catalytic upgrade of bio-oil to high-quality liquid fuel is always difficult. Herein, a high dispersed Ru/SiO 2 -ZrO 2 catalyst was prepared by polyol reduction method, which exhibited a high catalytic activity on hydrodeoxygenation (HDO) of the phenolic compounds and pyrolysis lignin-oil. As a model compound, guaiacol had a conversion of 99.9% and a cyclohexane selectivity of 96.7% at the temperature of 240 °C. This polyol Ru/SiO 2 -ZrO 2 catalyst had a good recyclability in the guaiacol HDO. No significant decrease on the catalytic activity was presented after continuous 5 runs, and the conversion kept close to 100% and the selectivity of cyclohexane kept above 88%. Several common phenolic compounds also enabled to be turned into corresponding cycloalkanes efficiently. The high Ru dispersion and the large amount of strong acid sites in the polyol Ru/SiO 2 -ZrO 2 are the main reasons for the good catalytic performances. The synergetic effects between high dispersed metal site and strong acid site lead to a promoted efficiency in the HDO process to form the cycloalkanes. This active catalyst was also applicable to the upgrade of pyrolysis lignin-oil. After reaction, the content of hydrocarbons in the lignin-oil increased from 16.2% to 86.1%, and guaiacols and other oxygenated compounds were almost disappeared. The upgraded lignin-oil has potential to be used as high-quality fuels directly.
研究领域:
催化化学
·
能源化学
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产品关联
DOI
高影响因子
Advanced Science
·
2023
·
IF 15.1
Huiwen Xiong,Chenxin Zhu,Changhao Dai,Xin Ye,Yuanyuan Li,Pintao Li,Shuang Yang,Ghazala Ashraf,Dacheng Wei,Hui Chen,Huali Shen,Jilie Kong,Xueen Fang
Early diagnosis of acute diseases is restricted by the sensitivity and complex process of sample treatment. Here, an ultrasensitive, rapid, and portable electrochemiluminescence-microfluidic (ECL-M) system is described via sandwich-type immunoassay and surface plasmonic resonance (SPR) assay. Using a sandwich immunoreaction approach, the ECL-M system employs cardiac troponin-I antigen (cTnI) as a detection model with a Ru@SiO 2 NPs labeled antibody as the signal probe. For miR-499-5p detection, gold nanoparticles generate SPR effects to enhance Ru(bpy) 3 2+ ECL signals. The system based on alternating current (AC) electroosmotic flow achieves an LOD of 2 fg mL −1 for cTnI in 5 min and 10 aM for miRNAs in 10 min at room temperature. The point-of-care testing (POCT) device demonstrated 100% sensitivity and 98% specificity for cTnI detection in 123 clinical serum samples. For miR-499-5p, it exhibited 100% sensitivity and 97% specificity in 55 clinical serum samples. Continuous monitoring of these biomarkers in rats' saliva, urine, and interstitial fluid samples for 48 hours revealed observations rarely documented in biotic fluids. The ECL-M POCT device stands as a top-performing system for ECL analysis, offering immense potential for ultrasensitive, rapid, highly accurate, and facile detection and monitoring of acute diseases in POC settings.
研究领域:
分析化学
·
生物医学工程
·
医疗诊断
·
纳米技术
·
生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）