JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2016
·Vol. 163
·期 7
·B280
·
IF 3.014
F. Shang,Y. Liu,S. Wang
A simple, economic, highly sensitive and highly selective electrochemiluminescence (ECL) sensor for the detection of tetrodotoxin (TTX) has been developed at Nafion-graphene-Ru(bpy)32+ composite modified glassy carbon electrode (GCE). Nafion can be used as a modified electrode material to achieve the one-step immobilization of graphene and Ru(bpy)32+ due to its good electrochemical stability and high ion selectivity. The modified glassy carbon electrode not only can be acted as a solid phase microextraction tool to perform the enrichment of TTX, but also can provide good stability of the ECL sensor. Under optimal conditions, the ECL intensity of the sensor increased proportionally to the logarithmic concentrations of TTX in the range of 0.5–5000 ng·mL−1 with a detection limit of 0.1 ng·mL−1. This one-step ECL sensor showed good performance in stability, reproducibility and precision.
研究领域:
分析化学
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毒理学
·
生物传感技术
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DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 12
·2754-2761
·
IF 7
Yu Ji,Shuang He,Yang Chen,Pei Zhang,Jing Sun,Ya Li,Kaida Kuang,Nengqin Jia
A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was proposed based on Ru(bpy)32+@HKUST-1/TPA and Ce2Sn2O7/K2S2O8 probes for detecting the NT-proBNP biomarker of heart failure. HKUST-1 with a high specific surface area facilitates the loading of more Ru(bpy)32+, effectively improving the anodic signal intensity, while the emerging Ce2Sn2O7 emitter displays a potential-matching cathodic emission with moderate intensity. Two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, XPS, FT-IR spectroscopy and UV-Vis diffuse reflectance spectroscopy. This dual-signal immunosensor has a wide linear range (5 × 10−4–1 × 104 ng mL−1) and a low quantitative detection limit, simultaneously showing high sensitivity, stability and reproducibility, as well as the detection capability of actual serum samples. This dual signal-calibrated immunoassay platform not only reduces the false positive rate of detection results but also provides a promising method for the early diagnosis of heart failure.
研究领域:
生物医学工程
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分析化学
·
心血管疾病诊断
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2020
·
IF 3.807
Luyao Su,Jie Mao,Sui Wang,Yufang Hu
Herein, the paper developed a bimodal electrochemiluminescence (ECL) method based on dual-enhancement Ru(bpy) 3 2+ /CQDs/AA system combined with magnetic field enhanced solid-phase microextraction (MFE-SPME) for the direct determination of ascorbic acid (AA). The nanocomposite of Fe 3 O 4 @CS@GO, as a magnetic adsorbent, could efficiently extract AA under the external magnetic field provided by the magnetic glassy carbon electrode (MGCE) with the extraction efficiency up to 89.2%. Based on carbon quantum dots (CQDs) as the co-reactant for Ru(bpy) 3 2+ , when the potential was swept cyclically between −3.5 and 2.0 V, the bimodal ECL emissions were obtained in the Ru(bpy) 3 2+ /CQDs/AA system and were enhanced as AA concentration increased. The method could quantitatively detect AA, because the ln(ECL-1/ECL-2) of bimodal ECL system was in a good linear relationship with the negative logarithm of the AA concentration in the range of 10 −5 –10 −9 M with the limit of detection (LOD, S/N = 3) of 330 pM. After a series of evaluations, the method exhibited good sensitivity, satisfactory repeatability and excellent anti-interference, demonstrating that the developed method had great application potential in analysis fields.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Yong-Ping Dong,Ying Zhou,Jiao Wang,Jun-Jie Zhu
In the present work, strong anodic electrogenerated chemiluminescence (ECL) of Ru(bpy)32+ was observed at a graphene oxide modified glassy carbon electrode (GO/GCE) in the absence of coreactants. The electrocatalytical effect of GO on the oxidation of Ru(bpy)32+ suggested that GO itself can act as the coreactant of Ru(bpy)32+ ECL, which can be used to fabricate the ECL biosensor. Thiol group terminated adenosine triphosphate (ATP) aptamer was immobilized on the GO film via DNA hybridization. When gold nanoparticles/graphene oxide (AuNPs/GO) nanocomposites were modified on the aptamer through the S–Au bond to form a sandwich-like structure, the ECL resonance energy transfer (ECL-RET) could occur between Ru(bpy)32+ and AuNPs/GO nanocomposites, resulting in an apparent decrease of ECL signal. After the ECL sensor was incubated in ATP solution, the AuNPs/GO nanocomposites were released from the electrode due to the specific interaction between aptamer and ATP, leading to the increased ECL signal. On the basis of these results, an ECL aptasensor was fabricated and could be used in the sensitive and selective detection of ATP in the range of 0.02–200 pM with a detection limit of 6.7 fM (S/N = 3). The proposed ECL aptasensor can be applied in the detection of ATP in real samples with satisfactory results.
研究领域:
分析化学
·
生物传感技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Fang-Kai Du,Hui Zhang,Xue-Cai Tan,Jun Yan,Min Liu,Xiao Chen,Ye-Yu Wu,De-Fen Feng,Quan-You Chen,Jian-Mei Cen,Shao-Gang Liu,Yu-Qin Qiu,He-You Han
In this work, the feasibility of a novel sensitive electrochemiluminescence aptasensor for the detection of lysozyme using Ru(bpy) 3 2+ [email protected] L -lysine-Au ( [email protected] ) nanocomposites labeling as an indicator was demonstrated. The substrate electrode of the aptasensor was prepared by depositing gold nanoparticles (AuNPs) on 3D graphene-modified electrode. The lysozyme binding aptamer (LBA) was attached to the 3D graphene/AuNPs electrode through gold-thiol affinity, hybridized with a complementary single-strand DNA (CDNA) of the lysozyme aptamer labeled by [email protected] as an electrochemiluminescence intensity amplifier. Thanks to the synergistic amplification of the 3D graphene, the AuNPs and [email protected] NPs linked to Ru(bpy) 3 2+ -ECL further enhanced the ECL intensity of the aptasensor. In presence of lysozyme, the CDNA segment of the self-assembled duplex was displaced by the lysozyme, resulting in decreased electrochemiluminescence signal. Under the optimized conditions, the decrease in electrochemiluminescence intensity varied proportionally with the logarithmic concentration of the lysozyme from 2.25 × 10 –12 to 5.0 × 10 −8 mol L −1 , and the detection limit was estimated to 7.5 × 10 –13 mol L −1 . The aptasensor was further tested in real samples and found reliable for the detection of lysozyme, thus holding great potential application in food safety researches and bioassay analysis.
研究领域:
分析化学
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生物传感器
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纳米技术
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食品安全
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生物分析
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Weiqiang Yang,Jiajing Xu,Qingda Yao,Xiaoguang Xu,Xiaoping Chen,Jiancong Ni,Qingxiang Wang,Zhenyu Lin
Prolidase (PLD) plays a crucial role as a dipeptidase in various physiological processes , specifically involved in the cleavage of proline-containing dipeptides for efficient recycling of proline . The accurate determination of PLD activity holds significant importance in clinical diagnosis. Herein, a solid-state electrochemiluminescence (ECL) biosensor was developed to address the urgent need for PLD assay. The Ru(bpy) 3 2+ was electrophoretically deposited within the nanochannels of vertically-ordered mesoporous silica film (VMSF) on indium tin oxide (ITO) electrodes. The Ru(bpy) 3 2+ -deposited VMSF/ITO (Ru-VMSF/ITO) exhibited a remarkable ECL response towards proline, attributed to the enhanced concentration of the reactants and improved electron transfer resulting from the nanoconfinement effect. As PLD specifically enzymolyzed the Gly-Pro dipeptide to release proline, a proline-mediated biosensor was developed for PLD assay. Increased PLD activity led to enhanced release of proline into the porous solid-state ECL sensors, resulting in a more robust ECL signal. There was a linear relationship between Δ ECL intensity and logarithmic concentration of PLD in the range of 10–10000 U/L, with a detection limit of 1.98 U/L. Practical tests demonstrated the reliability and convenience of the proposed bioassay, making it suitable for widespread application in PLD assays.
研究领域:
分析化学
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生物传感器
·
电化学
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纳米技术
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xue Dong,Guanhui Zhao,Li Liu,Xuan Li,Qin Wei,Wei Cao
In this work, Ru(bpy) 3 2+ encapsulated in metal–organic frameworks (MOFs) UiO-67 (Ru(bpy) 3 2+ /UiO-67) as luminophor was easily prepared and firstly applied in constructing an electrochemiluminescence (ECL) immunosensor to efficiently estimate diethylstilbestrol (DES). The competitive method-based ECL immunosensor platform was fabricated by amino-silicon dioxide which possesses large surface area . The poriness of UiO-67 was splendid so that Ru(bpy) 3 2+ could be easily encapsulated. Ru(bpy) 3 2+ /UiO-67 with excellent ECL luminescence signal existed large specific surface area for easily labeled with antibodies. DES competed with bovine serum albumin-diethylstilbestrol (BSA-DES) for binding to antibody-specific sites in the constructed immunosensor. However DES was micromolecule, which was easier to bond to antibodies than BSA-DES. The ECL signal was gradually decreases with the increase of the concentration of DES. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01 pg mL −1 to 50 ng mL −1 with a low detetion limit of 3.27 fg mL −1 (S/N = 3). The novel fabricated immunosensor with interference immunity and high stability may cause an attractive approach for the other targets determination.
研究领域:
分析化学
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环境科学
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生物技术
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DOI
高影响因子
ACS Applied Bio Materials
·
2019
·
IF 0
Zhixin Li,Jinwei Zhang,Huixing Chen,Xiaocui Huang,Da Huang,Fang Luo,Jian Wang,Longhua Guo,Bin Qiu,Zhenyu Lin
Hyaluronidase (HAase), a specific enzyme of hyaluronic acid (HA), has been reported as a potential tumor biomarker in recent years. Hence, developing some simple, rapid, and sensitive methods for HAase assay is necessary. In this work, a simple and sensitive biosensor constructed by a reliable controlled release system and a mature electrochemiluminescence (ECL) analytical technique has been devised for the quantification of HAase with high efficiency and selectivity. Tris (2,2′-bipyridyl) ruthenium(II) chloride hexahydrate doped SiO2 nanoparticles (Ru@SiO2 NPs), as ECL signal probes, were trapped in the hydrogel fabricated by HA and polyethylenimine evenly and steadily. When HAase existed, the hydrogel was decomposed by HAase, and the Ru@SiO2 NPs escaped from the hydrogel into the supernate. The ECL signal produced from the supernate can be detected and used to characterize HAase concentration. The result showed a good linear relationship between ECL intensity, and HAase concentration ranged from 2 to 60 U/mL and the limit of detection was 2 U/mL. The developed controlled release ECL biosensor has been used for HAase quantification in urine samples with satisfactory results.
研究领域:
分析化学
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生物化学
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生物技术
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医学诊断
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产品关联
DOI
PubMed
CHEMISTRY-A EUROPEAN JOURNAL
·
2024
·
IF 3.9
Jialian Ding,Bin Su
Graphical One plus one is greater than two: A remarkable electrochemiluminescence (ECL) enhancement of Ru(bpy) 3 2+ /TPrA is observed by introducing another coreactant, TEOA, with a 10.8-fold increase of ECL intensity and 1.7-fold extension of ECL layer. The enhancement was interpreted by a chemical oxidation route, in which TEOA + ⋅ acts as an enhancer to produce more TPrA + ⋅ and meanwhile allow the remote formation of TPrA + ⋅. This dual-coreactants strategy works not only in solution with freely diffusive Ru(bpy) 3 2+ but also on Ru(bpy) 3 2+ -functionalized microbeads, showing promise in ECL-based bioassay and microscopy. The electrochemiluminescence (ECL) of ruthenium(II) tris(2,2′-bipyridyl) (Ru(bpy) 3 2+ ) with tri- n -propylamine (TPrA) as the good coreactant can be unexpectedly enhanced by a weak coreactant, such as triethanolamine (TEOA). First, the intensity of ECL emitted by Ru(bpy) 3 2+ /TPrA can be remarkably amplified by 10.8-fold after adding some amount of TEOA. Moreover, the ECL layer thickness, measured by self-interference spectroscopy, is also doubled. The enhancement far exceeding the superposition of respective contribution of TPrA and TEOA was elucidated by a “chemical oxidation mechanism”, in which TEOA + ⋅ acts as a chemical enhancer to oxidize TPrA in solution and to accelerate significantly the ECL reaction kinetics. This mechanism was proved by single-photon counting experiment and finite element simulations. In addition, the dual-coreactants strategy works well not only in solution with freely diffusive Ru(bpy) 3 2+ , but also on Ru(bpy) 3 2+ -functionalized microbeads, suggesting that ethanolamines could act as cheap, easily available and low-background enhancers for ECL-based bioanalysis and microscopy.
研究领域:
分析化学
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生物分析
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显微技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Zheng Shen,Rui Xu,Guangxian Wang,Haifang Wang,Deyan Xu,JingJing Liu,Guanjie Wang,Yemin Guo,Xia Sun
In this study, a signal amplification strategy was developed, which tris (2, 2′-bipyridyl) ruthenium (II) (Ru(bpy) 3 2+ ) was incorporated as a guest molecule into the preparation system of lanthanide-based metal-organic framework (MOF). This strategy relied on strong electrostatic interactions and coordination competition to influence the growth process of the MOF, resulting in the firm immobilization of Ru(bpy) 3 2+ within the MOF structure. The spatial confinement effect of the MOF effectively improved the efficiency of electron transfer in the electrochemiluminescence (ECL) reaction. The constructed ECL aptasensor using this MOF exhibited excellent performance, with limits of detection (LODs) for four organophosphorus pesticides (OPs), phorate, profenofos, isocarbophos and omethoate, as low as 0.0482 ng/mL, 0.0093 ng/mL, 0.0085 ng/mL, and 0.0893 ng/mL, respectively. This work has paved the way for a clever strategy of immobilizing Ru(bpy) 3 2+ molecules and amplifying signals, expanding the application of dual-ligand MOF, which provides valuable insights for future biosensor design.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2011
·
IF 2.78
Haiyan Wang,Dongyan Sun,Zhian Tan,Wu Gong,Lun Wang
In this work, an electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of α-fetoprotein (AFP) was fabricated using Ru(bpy) 3 2+ -encapsulated liposome as the label and electrodeposited gold nanoparticles (GNPs) as the immobilizing support. Great signal amplification was achieved since liposome could encapsulate large amount of reporter molecules and GNPs could provide large active surface. Under optimized conditions, with sandwich type format, a linear range of AFP from 0.005 to 0.2 pg/mL and an extremely low detection limit of 0.001 pg/mL was obtained, much lower than that in previous reports. The proposed ECL immnuosensor showed high sensitivity, specificity, and good stability, which may open a new door to ultrasensitive detection of proteins in clinical analysis.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Wang Daifang,Shen Ligong,Liu Wenjun,Cao Xiao,Wang Qianwen
This article introduces a novel unlabeled surface-enhanced electrochemiluminescence (SEECL) sensor for malachite green (MG) detection. The SEECL sensor was prepared by modifying the Ru(bpy) 3 2+ doped gold-SiO 2 core–shell nanocomposites (Au@SiO 2 -Ru(bpy) 3 2+ ) on the gold electrode. Ru(bpy) 3 2+ of nanocomposites can not only emit electrochemiluminescence (ECL) with electrochemical reaction, but also induce the local surface plasmon resonance (LSPR) of gold core. That is beneficial to enhance the ECL signa of sensor. However, in the existence of MG, the luminescence of sensor would be quenched by the fluorescence resonance energy transfer (FRET) between MG and Ru(bpy) 3 2+ . In this paper, both fluorescence and ECL of the Au@SiO 2 -Ru(bpy) 3 2+ were investigated for MG detection. And the results show that the SEECL sensor has high sensitive to MG. Under the optimal experimental conditions, the minimum detection concentration could be achieved about 1.0 nM of MG, which fully meets the China national standard detection requirements of veterinary drug residue in seafood.
研究领域:
分析化学
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环境科学
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食品安全
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）