Food Chemistry-X
·
2024
·
IF 6.5
Hua Zheng,Linlin Feng,Zheng Huang,Ziwei Zou,Xiaolong Ma,Ziping Pan,Jinfeng Li,Jinxia Wu,Mei Li,Zhiheng Su
Aflatoxin B1 (AFB1) is a potent toxin in food, necessitating rapid, instant, and sensitive detection. We have engineered an electrochemical sensor to monitor AFB1 using a system composed of Fe 3 O 4 -NH 4 /AuNPs/apt-S1. The aptamer specifically recognizes AFB1, while ‘S1’ is functionalized with methylene blue to enhance the current. The RecJf exonuclease promotes the formation of the electrochemical strategy. The Fe 3 O 4 component, with its magnet properties, enables a rapid separation of solids and liquids without the need for instrumentation. The sensor exhibits a linear range for AFB1 ranging from 1 ng to 10 μg. The regression equation is I(nA) = 446.8 × logc+2085 (where I and c represent the peak current and AFB1 concentration, respectively). The correlation coefficient is 0.9508, and the detection limit is 3.447 nM. The relative standard deviation of AFB1 in peanut oil ranges from 4.80% to 6.80%. These results demonstrate that the sensor has high sensitivity, stability, repeatability, and specificity for AFB1 detection.
研究领域:
食品安全
·
分析化学
·
电化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 31
·期 19
·195705
·
IF 3.551
Chen Wang,Qinghua Yi,Qiang Zhang,Fumao Wang,Guifu Zou
Cu2BaSn(S1−xSex)4 has shown great prospects in the photoelectric field due to Earth-abundance, low toxicity, cost efficiency, direct bandgap, high absorption coefficient (>104 cm−1) and reduced anti-site disorder relative to Cu2ZnSn(S1−xSex)4. A fully-tunable ratio of S/Se is the key to broaden the bandgap of Cu2BaSn(S1−xSex)4. Here, we introduce a thionothiolic acid metathesis process to readily tune the stoichiometry of Cu2BaSn(S1−xSex)4 films for the first time. Different stoichiometric Se/(S + Se) of Cu2BaSn(S1−xSex)4 from zero to one can vary the bandgap range from 2 to 1.68 eV. The grain size of Cu2BaSn(S1−xSex)4 films can be grown more than 10 μm. The optimized bandgap and high-quality growth of Cu2BaSn(S1−xSex)4 films ensure the best power conversion efficiency of 2.01% for solution-processed Cu2BaSn(S1−xSex)4 solar cells. This method provides an alternative solution-processed way for the synthesis of fully stoichiometric Cu2BaSn(S1−xSex)4.
研究领域:
材料科学
·
太阳能电池技术
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Hong Zhang,Chuance Cheng,Nalin Dong,Xiaoming Ji,Jiandong Hu
S1 nuclease plays an important role in DNA transcription, recombination and repair. Here, we synthesized a positively charged [email protected] core-shell nanoparticles ((+) [email protected] CSNPs) and used as highly stable and enhanced fluorescence quenching platform for one-step S1 nuclease activity detection. In the sensor, fluorophore labeled DNA was used as signal probe and its fluorescence signal could be effectively quenched based on the electrostatic interaction of it with (+) [email protected] CSNPs. In the presence of S1 nuclease, DNA probe was hydrolyzed into DNA fragments and cannot interact with (+) [email protected] CSNPs, inducing an obvious fluorescence signal increase that was proportional with S1 nuclease concentration in the range of 2.5 × 10 −4 –3.0 × 10 −2 U/mL. The enhanced quenching efficiency of (+) [email protected] CSNPs paved the way for sensitive S1 detection with a detection limit of 6.0 × 10 −5 U/mL. Moreover, the high stability of such nanocomposite can achieve the one-step detection of S1 in complex solution containing salt, protein or other metal ions. In addition, the method also exhibited high selectivity for nuclease detection, making our sensing platform has a potential application value in bioanalysis and clinical diagnosis.
研究领域:
生物化学
·
分析化学
·
医学诊断
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产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2017
·Vol. 22
·期 2
·347-353
·
IF 2.316
Lan Zhang,Chen Xin,Zhang Sheng,Wu Jihuai
Three-dimensional TiO 2 hierarchical nanostructures (3D-TiO 2 -HNs) containing TiO 2 nanotrees and nest-like hollow spheres were synthesized and used as backbones for CdSe
x
S 1− x
quantum dot (QD) loading. These CdSe
x
S 1− x
QD-sensitized 3D-TiO 2 -HNs were then used as photoelectrodes in the preparation of quantum-dot-sensitized solar cells. As revealed by TEM images, the highly porous 3D-TiO 2 -HNs represent an excellent framework on which to deposit a large number of CdSe
x
S 1− x
QDs in order to form homogeneous and compact CdSe
x
S 1− x
-sensitized layers in photoelectrodes using a spin-assisted successive ionic layer adsorption and reaction method (spin-SILAR). Following careful adjustment of the molar ratio of Se 2− to S 2− , the number of spin-SILAR cycles, and the thickness of the CdS passivation layer used, the best-performing QDSC was shown to yield a short-circuit current density of 18.22 mA cm −2 , an open-circuit voltage of 0.520 V, a fill factor of 0.510, and a power conversion efficiency of 4.83%. This high performance is possible because the device is able to absorb a relatively broad range of wavelengths and because charge recombination is suppressed in the device.
研究领域:
材料科学
·
纳米技术
·
能源研究
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Hong Yao,Lei Wang,Xin Tang,Zhuo Yang,Huanyong Li,Chongzhen Sun,Xiyang Wu,Dan Xu
In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified. Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined. Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model. Results showed that S1 and S2 have different impacts on human gut microbiota in vitro . S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid. Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably. We concluded that S1 and S2 may have potential prebiotic functions.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Energy Chemistry
·
2024
·
IF 14
Minmin Wang,Chao Feng,Xin Zhang,Ping He,Houyu Zhu,Yunqi Liu,Yuan Pan
The development of atomically dispersed platinum-based catalysts with high performance and well-defined active site structures is crucial for the commercialization of water electrolysis for hydrogen production. Herein, we propose a coordination dual-shell synergistic regulation mechanism of coal pitch-derived carbon-supported single atom Pt-N x O y -S 1 catalytic sites by a self-assembly-pyrolysis strategy for promoting hydrogen evolution reaction (HER). The Pt-N 3 O 1 -S 1 sites exhibited the highest HER performance, with an overpotential of 92 mV at a current density of 400 mA cm −2 . At 50 mV, the turnover frequency was 34.04 s −1 and the mass activity was 22.83 A mg Pt −1 , which is 63.4 times that of the 20% Pt/C catalyst. Theoretical calculations revealed that the coordination dual-shell impacts the electronic structure of the Pt atoms and the adsorption strength towards reactants synergistically. The S atoms in the second coordination shell weakened the strength of Pt-N first shell, resulting the more surface valence electrons around Pt atoms, exhibiting the most suitable adsorption free energy and enhancing the adsorption of H + on Pt-N 3 O 1 -S 1 sites, thus enhancing the electrocatalytic HER process by promoting Volmer step. This work reveals that coordination dual-shell synergistic regulation is an effective strategy for enhancing the electrocatalytic reaction process.
研究领域:
材料科学
·
催化化学
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产品关联
DOI
高影响因子
VETERINARY RESEARCH
·
2025
·Vol. 56
·期 1
·1-14
·
IF 3.5
Li Peiyao,Su Rina,Ren Ganglin,Si Hongbin,Song Xingju,Hu Dandan
The apicomplexan parasite Eimeria is the causative agent of coccidiosis, which results in enteritis in animals and imposes a significant economic burden on the farming industry. Apicomplexan Apetala2/ERF (ApiAP2) transcription factors are known to play crucial roles in various processes of Apicomplexa parasites. However, little is understood regarding the role of ApiAP2s in Eimeria species. In this study, we performed a functional characterisation of ApiAP2 through CRISPR-Cas9-mediated knockout. Our results revealed that the parasite’s invasion efficiency, total oocyst output, and virulence were significantly impaired after EtAP2-S1 depletion. RNA sequencing (RNA-Seq) and Cleavage Under Targets and Tagmentation (CUT&Tag) analyses showed that EtAP2-S1 targets the promoters of numerous genes, and its knockout resulted in the up-regulation of 59 sag genes. Furthermore, the knockout strain exhibited significantly lower virulence while offering excellent immune protection, making it a promising candidate for a vaccine. This study demonstrates that EtAP2-S1 is a fitness-conferring gene that suppresses the expression of sag genes in E. tenella , which is the first step in developing gene-knockout vaccines to control coccidiosis.
研究领域:
分子生物学
·
寄生虫学
·
免疫学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
·
2026
·
IF 1.8
Enyuan Chen,Yu Yin,Xiaoyun Miao,Bin Yu,Jun Yang,Mingming Li,Xiaoyun Wang,Caifang Ni
Objective: To develop S1-4 aptamer-conjugated citric acid-coated Fe3O4 nanoparticles and evaluate their targeting ability and magnetic hyperthermia effect against MCF-7 breast cancer cells.Methods: Citric acid-coated Fe3O4 nanoparticles were synthesized by chemical co-precipitation and subsequently conjugated with the S1-4 aptamer to fabricate S1-4 aptamer-conjugated citric acid-coated Fe3O4 nanoparticles. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, dynamic light scattering, zeta potential analysis, Fourier transform infrared spectroscopy, and vibrating sample magnetometry. Biocompatibility was assessed in MCF-7 and MEF cells; cellular targeting was examined by Prussian blue staining; and the magnetic hyperthermia effect under an alternating magnetic field was evaluated by cell viability, live/dead staining, and apoptosis assays.Results: Compared with citric acid-coated Fe3O4 nanoparticles, S1-4 aptamer-conjugated citric acid-coated Fe3O4 nanoparticles exhibited a larger hydrodynamic diameter (17 ± 4 nm vs. 8 ± 3 nm) and a more negative surface charge (−26.7 ± 0.3 mV vs. −19.7 ± 0.5 mV). Fourier transform infrared spectroscopy confirmed successful aptamer conjugation. Both nanoparticles demonstrated good biocompatibility in MCF-7 and MEF cells. Prussian blue staining demonstrated stronger cellular uptake of S1-4 aptamer-conjugated citric acid-coated Fe3O4 nanoparticles in MCF-7 cells. Under alternating magnetic field exposure, S1-4 aptamer-conjugated citric acid-coated Fe3O4 nanoparticles produced a significantly greater hyperthermia effect than the controls, resulting in reduced cell proliferation and increased apoptosis in MCF-7 cells (P < 0.01).Conclusions: S1-4 aptamer-mediated Fe3O4 nanoparticles demonstrated active targeting ability and enhanced the antitumor effect of alternating magnetic field–mediated magnetic hyperthermia in breast cancer cells and maintained low cytotoxicity under the tested conditions, suggesting that they may be a feasible platform for targeted magnetic hyperthermia.
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS CHARACTERIZATION
·
2020
·
IF 3.562
Shuai Ma,Fengjin Xia,Lifeng Dong,Ruibin Jia,Zhenzong Sha,Jie Wang,Yingjie Chen,Beili Pang,Hongzhou Dong,Liyan Yu
Coupling plasmonic gold nanoparticles to sub-stoichiometric copper chalcogenide nanocrystals tends to generate promising nano-heterostructures. We report the research on plasmon-enhanced Cu 2 ZnSn(S 1 − x ,Se x ) 4 -Au nanocomposites (CZTSSe for 0 < x < 1, and CZTS if x = 0), emphasizing on exploring how hetero-epitaxial relationship at Cu 2 ZnSn(S 1 − x ,Se x ) 4 //Au interfaces affects their optoelectronic properties. Digital image processing tools, fast Fourier transform/Fourier inversion, are utilized to extract interfacial epitaxial orientations. We demonstrate that nonnegligible lattice mismatch in short-range order could be equivalently compensated by coincident lattice-matching at certain periodic intervals in a long-range order. By exploring polytypic CZTSSe, we further provide reasonable interpretation of abnormal symmetrical distribution of Au on CZTSSe, which induces lower gold coverage rate and accordingly moderates their localized surface plasmon resonance effect, compared to that of CZTS. It indicates that the shape and phase of CZTSSe should be elaborately controlled to realize optimal multi-site nucleation of heterostructured nanocomposites. As a result, by testing optoelectronic response of solar cells with Cu 2 ZnSn(S 1 − x ,Se x ) 4 -Au films as counter electrodes (CEs), we confirm that a superior hetero-epitaxial interconnection between Au and Cu 2 ZnSn(S 1 − x ,Se x ) 4 can availably restrain interfacial defects of the junction and thus contribute efficient hole transfer/transportation in cathode region. The corresponding balanced electron and hole migration plays a quite significant role in the improvement of open-circuit voltage ( V oc ) and fill factor of photovoltaic devices .
研究领域:
材料科学
·
纳米技术
·
光伏技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2013
·
IF 12.062
Feng-Jia Fan,Liang Wu,Ming Gong,Guangyao Liu,Yi-Xiu Wang,Shu-Hong Yu,Shiyou Chen,Lin-Wang Wang,Xin-Gao Gong
The wurtzite-derived Cu2ZnSn(S1–xSex)4 alloys are studied for the first time through combining theoretical calculations and experimental characterizations. Ab initio calculations predict that wurtzite-derived Cu2ZnSnS4 and Cu2ZnSnSe4 are highly miscible, and the band gaps of the mixed-anion alloys can be linearly tuned from 1.0 to 1.5 eV through changing the composition parameter x from 0 to 1. A synthetic procedure for the wurtzite-derived Cu2ZnSn(S1–xSex)4 alloy nanocrystals with tunable compositions has been developed. A linear tunable band-gap range of 0.5 eV is observed in the synthesized alloy nanocrystals, which shows good agreement with the ab initio calculations.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Yingfeng Qin,Yefei Ma,Xue Jin,Liangliang Zhang,Gaojie Ye,Shulin Zhao
As an important antitumor drug, bleomycin (BLM) is widely used in the treatment of a variety of cancers. In addition, nucleases play a crucial role in DNA replication, recombination and repair which are associated with cancer development. Thus, the development of BLM and nuclease detection methods is of great significance in cancer therapy and related biological mechanism research. Here, a WS 2 nanosheet-based turn-on fluorescent sensing platform for simple, fast and sensitive detection of BLM and nuclease was reported. WS 2 nanosheet exhibits different affinity toward ssDNA with different length and excellent fluorescence quenching ability. A fluorescein (FAM)-labeled long ssDNA could be adsorbed on the surface of WS 2 nanosheet and the fluorescence was therefore quenched. In the presence of BLM·Fe(II) or S1 nuclease (a ssDNA-specific nuclease which was used as a model enzyme), an irreversible scission of long ssDNA was underwent through the BLM-induced oxidation cleavage or S1 nuclease-induced enzymatic hydrolysis. Short FAM-linked oligonucleotide fragments which could not be adsorbed on the nanosheet surface were then produced, resulting in a weak fluorescence quenching after mixing WS 2 nanosheets. Thus, the fluorescence signal was restored. The proposed sensor displays a wide linear range and a high sensitivity with a detection limit of 0.3 nM for BLM and 0.01 U mL −1 for S1 nuclease. It also exhibits a good performance in complex biological samples. This method not only provides a strategy for BLM or S1 nuclease assay but also offers a potential application in biomedical and clinical study.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 11
·1851
·
IF 5.1
Lin Yuan,Jianfeng Yuan,Chen Gao,Haoming Zhao,Chengye Wu,Zhong-Hua Yang
The microbial conversion of inorganic Se into bioactive organoselenium compounds represents a cutting-edge strategy for developing functional foods with enhanced nutritional value. This study introduces Lactiplantibacillus plantarum S1, a novel Se-enriched probiotic strain isolated from traditional Chinese sauerkraut, and systematically optimizes its capacity for selenite biotransformation. Critical fermentation parameters—including sodium selenite supplementation timing (2 μg/mL added at mid-log phase, 7 h post-inoculation), pH (5.0), and anaerobic cultivation duration (12 h)—were identified as key determinants of conversion efficiency. The optimized protocol achieved a 72.3% organoselenium conversion yield, producing 626.6 μg/g cellular organoselenium while maintaining probiotic viability (2.28 × 10 9 CFU/mL). Se speciation analysis demonstrated that 78.51% of intracellular Se existed in organic forms, with protein-bound Se constituting the predominant fraction (85.33%), followed by polysaccharide-associated (6.42%) and nucleic acid-linked (3.38%) species. The strain’s dual functionality as both an efficient Se bioconverter and a resilient probiotic carrier highlights its potential for nutraceutical applications. These findings not only establish a robust bioprocess for Se-enriched probiotic production but also reveal mechanistic insights into preferential Se incorporation into protein matrices. This work bridges microbial Se metabolism research with scalable functional food innovation, offering a sustainable platform for developing Se-fortified products with dual health benefits.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）