PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2015
·
IF 2.014
Baoyan Zhang,Zhiping Xu,Yixi Zhang,Xusheng Shao,Xiaoyong Xu,Jiaogao Cheng,Zhong Li
Fipronil is the first phenylpyrazole insecticide widely used in controlling pests, including pyrethroid, organophosphate and carbamate insecticides. It is generally accepted that fipronil elicits neurotoxicity via interactions with GABA and glutamate receptors, although alternative mechanisms have recently been proposed. This study evaluates the genotoxicity of fipronil and its likely mode of action in Drosophila S2 cells, as an in vitro model. Fipronil administrated the concentration- and time-dependent S2 cell proliferation. Intracellular biochemical assays showed that fipronil-induced S2 cell apoptosis coincided with a decrease in the mitochondrial membrane potential and an increase reactive oxygen species generation, a significant decrease of Bcl-2 and DIAP1, and a marked augmentation of Cyt c and caspase-3. Because caspase-3 is the major executioner caspase downstream of caspase-9 in Drosophila , enzyme activity assays were used to determine the activities of caspase-3 and caspase-9. Our results indicated that fipronil effectively induced apoptosis in Drosophila S2 cells through caspase-dependent mitochondrial pathways.
研究领域:
毒理学
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细胞生物学
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昆虫学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaoyu Zheng,Lin Xing,Xuemei Zhou,Yulian Tang,Zhen Liu,Xueting Zhang,Lei Hu,Zhengquan Yan
To monitor toxic NO 2 − and S 2− conveniently and effectively in 100% aqueous, nano CeO 2 was identified and modified first onto 2-dimensional Co 3 O 4 substrate (CeO 2 @2D Co 3 O 4 ) under microwave-assisted conditions at 140 °C. The obtained CeO 2 @2D Co 3 O 4 expressed excellent stability and superior oxidase-mimic activity with a wide temperature range from 20 to 60 °C. Under the optimized conditions (pH 4.0, 25 °C, 0.07 mM TMB), CeO 2 @2D Co 3 O 4 could accelerate the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue oxidation (oxTMB) within 12 min at room temperature , whose Michaelis constant ( K m ) and maximal reaction rate ( v max ) were 0.017 mmol/L and 2.17 × 10 −7 mol/L·s –1 respectively. Especially, both NO 2 − and S 2− could exclusively make the blue CeO 2 @2D Co 3 O 4 -TMB system into colorless and green respectively, expressing significant visibility. When applied for visual detection of NO 2 − and S 2− in real water, soil, drink and food samples, the recoveries were 97.6~104.6% and 97.5~104.1% with low detection limits of 3.26 × 10 −9 mol/L and 6.65 × 10 −8 mol/L (S/N = 3), far below the maxima in drinking water permitted by World Health Organization (WHO). The strengthened oxidase-mimic activity of CeO 2 @2D Co 3 O 4 and its different recognition mechanisms to NO 2 − and S 2− were studied in detail.
研究领域:
环境科学
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生物化学
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材料科学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 27
·11983-11991
·
IF 3.591
Wen Lu,Jiuzhou Shi,Jichao Chen,Lu Sun,Lingcen Shao,Hongyu Ren,Mengmeng Huang,Yanqin Wang,Shilong Yang,Xu Li
This study describes the synthesis of a coumarin-based reversible fluorescent probe BuCAC for the detection of Cu2+ and S2− in CH3CN : PBS (v/v = 8 : 2, pH = 7.4) solution. The resulting BuCAC exhibited high sensitivity (detection limit = 3.03 × 10−7 M) and selectivity towards Cu2+ through a 2 : 1 binding mode. In the presence of S2−, the BuCAC–Cu2+ recovered to BuCAC and CuS, which in turn perform the function of a sensitive probe with a lower detection limit of about 1.7 × 10−7 M. This “on–off–on” process can easily occur in 1 min with a repetition of at least 5 times. The sensing mechanism was confirmed by Job's plot analysis, MS, and density theory calculation. Besides, fluorescence imaging in zebrafish, HeLa cells, and soybean root tissue revealed that the probe BuCAC could serve as a valuable tool for monitoring and tracking intracellular Cu2+ and S2− while benefiting from its excellent fluorescence performance and lower cytotoxicity.
研究领域:
分析化学
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生物化学
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生物医学工程
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细胞生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2022
·
IF 7.139
Liangyu Tong,Yunpeng Liu,Chenyu Song,Yuqing Zhang,Sanjay S. Latthe,Shanhu Liu,Ruimin Xing
Hollow hybrid heterostructures are regarded to be promising materials as bifunctional electrocatalysts for highly efficient water electrolysis due to their intriguing morphological features and remarkable electrochemical properties . Herein, with FeNi-PBA as both a precursor and morphological template, we demonstrate the rational construct of cost-effective (Fe,Ni)S 2 @MoS 2 /NiS 2 hollow hybrid heterostructures as bifunctional electrocatalysts for alkaline overall water splitting. Microstructural analysis shows that the hybrid is a kind of hierarchical heterostructure composed of MoS 2 /NiS 2 nanosheets/nanoparticles in situ grown on hollow (Fe,Ni)S 2 nanocubes with abundant heterointerfaces, which effectively maximizes the electrochemical active sites to the accessible electrolyte ions , leading to the promoted charge transfer. As expected, the hybrid shows remarkable alkaline electrocatalytic performance, such as hydrogen evolution overpotential of 176 mV and oxygen evolution overpotential of 342 mV at 50 mA cm −2 , as well a cell voltage of 1.65 V at 20 mA cm −2 . Moreover, the stability and durability are greatly enhanced under harsh electrochemical conditions. This study opens a new venue for developing earth-abundant bifunctional electrocatalysts with hollow hybrid heterostructures for alkaline water electrolysis in the future.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Feijin Zhou,Wenying Cui,Chenggang Liu,Cheng Yao,Chan Song
The excessive presence of S 2− poses siganificant risks to human health and the environment. Therefore, the development of novel S 2− sensing methods remains crucial in daily life. Herein, Fe–N hollow mesoporous carbon nanospheres (Fe–N HMCNSs), characterized by a high specific surface area and excellent stability, were successfully prepared. Further investigation revealed that the colorimetric substrates such as 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonate) (ABTS), o-phenylenediamine (OPD), and 3,3′,5,5′-tetramethylbenzidine (TMB), can be effectively oxidized by Fe–N HMCNSs into colored products. Based on the oxidase-like activity of Fe–N HMCNSs, a facile colorimetric method for S 2− assay was constructed for the first time. The recognition of S 2− could be fulfilled by recording the absorbance intensity and observing color changes in nanozymes-based catalytic systems. This method is simple, cost-effective, and environment-friendly, offering a wide linear range (0.25–25 µM) and a low detection limit (42.2 nM) for S 2− assay. Furthermore, integration of smartphone-based RGB analysis enabled the portable and convenient S 2− sensing using the developed colorimetric sensor. The remarkable stability of Fe–N HMCNSs under harsh conditions endows their potential applications in the fields of food safety and environmental protection.
研究领域:
环境科学
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jihong Chen,Xiaoyu Dong,Dali Zhuo,Jiayuan Tang,Xiaoli Xiong,Hong Luo,Zhirong Zou
Metal-organic frameworks (MOFs) are porous materials that constructed by various metal nodes and organic ligands, taking the advantages of high porosity, large specific surface areas and more reactive sites etc. However, the applications of MOFs in hydride generation (HG)/chemical vapor generation (CVG)-colorimetric systems are rare. Herein, a series of silver-based MOFs (Ag-MOFs) were synthesized and served as substrates for As(III) and S 2- colorimetric analysis. As(III) and S 2- were converted into its volatile species (AsH 3 and H 2 S) via HG and CVG process, respectively, providing merits of low matrix interferences (selectivity) and high vapor generation efficiency (sensitivity). Ag-MOF-3 exhibits the best performances for As(III) and S 2- analysis, its reaction mechanisms were further explored. Ag nanoparticles (redox reaction) and Ag 2 S nanoparticles (combination reaction) were formed after Ag-MOF-3 reacted with AsH 3 and H 2 S, respectively, leading to color change from light yellow to black. The variation was recognized by naked eye and smartphone. Standard cards/curves of visual method and smartphone RGB method were thus established for As(III) and S 2- . It is a potential analytical method for on-site and rapid analysis of As(III) and S 2- with merits of low-cost, easy-to-use and portability.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Fei He,Shihai Zhang,Yanan Li,Xia Chen,Zhenyun Du,Chenghao Shao,Kan Ding
The fruit of Lycium ruthenicum Murr is used as traditional medicine and functional food . Previously we reported that one RG-I pectin from this fruit might inhibit pancreatic cancer cells growth. We further hypothesized that there might be other type of polysaccharides in this fruit also have anti-tumor effect. Here, we showed novel structure of a homogeneous polysaccharide named LRP1-S2 from this fruit and its anti-pancreatic cancer effect. Structure analyses suggested that LRP1-S2 was a novel arabinogalactan with the molecular weight ( Mw ) of 17.0 kDa. Bioactivity test showed that LRP1-S2 might attenuate the proliferation of pancreatic cancer cells in vitro and in vivo without significant cytotoxicity to normal pancreatic HPDE6-C7 cells and LO2 liver cells. Mechanism study indicated that it might induce apoptosis of BxPC-3 by inactivating P38 MAPK/NF-κB and GSK-3β/β-Catenin signaling pathways . These results suggested that LRP1-S2 could be a potential anti-tumor leading compound for functional food and new drug development. Chemical compounds arabinogalactan, pectin, galactan , arabinan , RN-1, HH1-1, LRP1-S2, LRP3-S1.
研究领域:
药理学
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癌症研究
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分子生物学
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植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Tong Cheng,Qi-Bo Wang,Qin-Qin Xu,Zhen-Hua Han,Jian-Zhong Yin
Mo doping optimizes the electronic structure of ReS2. MoxRe(1–x)S2/MoS2 alloy heterojunction exhibits more favorable application prospects in photodetection due to its higher electrical conductivity than ReS2/MoS2 heterojunction. However, alloy heterojunctions are difficult to prepare controllably using conventional vapor-phase chemical vapor deposition (CVD), and the heterojunction growth mechanism remains unclear. Here, a vapor–liquid–solid temperature-gradient process is proposed to grow alloy heterojunctions within predeposited molten Mo precursor droplets. The sulfuration reaction between Re diffusing into the droplet and Mo atoms facilitates the formation of alloy structures. The growth temperatures TRe and TMo in the temperature-gradient significantly affect the growth patterns and morphology evolution of the heterojunction. The MoS2 morphology in vertical alloy heterojunctions becomes triangular as the growth temperature increases. The dimension of the top MoxRe(1–x)S2 alloy is positively correlated with the Re diffusion concentration. Moreover, lateral alloy heterojunctions and single alloys are formed at lower and higher growth temperature differences between TRe and TMo, respectively. These results provide a controllable strategy for the synthesis of isotropic/anisotropic van der Waals TMDC heterojunctions.
研究领域:
材料科学
·
纳米技术
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半导体物理
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yilin Li,J.M. Cao,Linlin Li,Zeyu Yuan,Dongdong Li,Junzhi Li,Yuming Zhang,Hao Xu,Wei Han,Lili Wang
Materials used for conventional conversion-type anodes usually suffer from poor charge transfer and short lifespan, thereby limiting their utility in sodium-ion batteries (SIBs). To prevent these issues, the development of new anode materials is imperative. Herein, we used a synergistic modification strategy based on pseudo-isotopic substitution and 2D conductive skeleton support, to successfully prepare an unprecedented (Co 0.5 Ni 0.5 )S 2 @V 4 C 3 T x with carbon layer coating (C@NCS@V 4 C 3 T x ) anode materials for SIBs. We noted that the C@NCS@V 4 C 3 T x hybrid exhibited an ultrahigh reversible specific capacity of 705.6 mAh g −1 at 0.1 Ag −1 , and the corresponding reversible capacity that is 100-times higher (10 A g −1 ) at a specific heat capacity of 347.6 mAh g −1 , both higher than those of same kind batteries. Furthermore, the as-prepared hybrid also shows satisfactory long-term cycling stability, resulting from 2D skeleton V 4 C 3 T x MXene with high conductivity and robustness. Through in-situ characterization techniques and density functional theory calculations, the sodium ions storage mechanism was well-investigated, specifically, the synergy effect between the high capacity of bimetallic TMS and metallic conductivity and excellent stability of V 4 C 3 T x MXene plays an important role in high-performance achievement. Thus, assembled C@NCS@V 4 C 3 T x //Na 3 V 2 (PO 4 ) 3 full-cell delivers outstanding electrochemical properties that can be useful as a portable integrated unit for self-powered systems.
研究领域:
材料科学
·
电化学
·
储能
·
纳米材料
·
电池技术
✓ 已验证
DOI
高影响因子
BIODEGRADATION
·
2025
·Vol. 36
·期 4
·1-15
·
IF 3.2
Shao Pengfei,Yao Jun,Li Miaomiao,Min Ning
Phthalate esters (PAEs) are widely used as plasticizers and beneficiation flotation agents in ore smelters, which are ubiquitously distributed emerging contaminants in the environment. The biodegradation of PAEs by degrading microbes is a promising method for their remediation. In this study, we isolated a novel PAE-degrading bacteria, Sinomonas sp. S2, from a contaminated area of a metal(loid) smelter in Guangxi Zhuang Autonomous Region, China. Strain S2 is capable of degrading short-chain PAEs, including dimethyl phthalate (DMP), diethyl phthalate (DEP), di-iso-butyl phthalate (DIBP) and di-n-butyl phthalate (DBP). Sinomonas sp. S2 can completely degrade DBP at concentrations of 400 mg·L −1 within 24 h. The degradation kinetics of PAEs followed the modified Gompertz model. Strain S2 demonstrated good environmental adaptability thriving at pH ranging from 5 to 9 and temperatures between 20 and 40 °C, indicated by its growth on DBP. The optimal pH and temperature for degradation were found to be 7 and 40 °C, respectively. Additionally, several metabolites of DBP were identified, including phthalic acid (PA), butyl acetate, ethyl propionate and methyl 2-methylbutyrate. The reconstructed degradation pathway of DBP may involve protocatechuic acid, β-carboxy-cis, cis-mucronate and γ-carboxy muconolactone, ultimately leading to the tricarboxylic acid (TCA). In a bioaugmentation experiment involving soil artificially contaminated with DBP, strain S2 could promote the degradation of DBP in soil. The results indicate that strain S2 had high degradation capacity and environmental tolerance, which had the potential to be applied in the bioremediation of DBP-contaminated environments.
研究领域:
环境科学
·
微生物学
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生物技术
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土壤学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2024
·
IF 5.7
Qi WeiYu,Yu YouLi,Yang ChenChen,Wang XiaoJuan,Jiang YuChen,Zhang Li,Yu ZhengQing
Toxoplasma gondii (T. gondii) is a zoonotic disease that poses great harm to humans and animals. So far, no effective T. gondii vaccine has been developed to provide fully protection against such parasites. Recently, numerous researches have focused on the use of poly-lactic-co-glycolic acid (PLGA) and chitosan (CS) for the vaccines against T. gondii infections. In this study, we employed PLGA and CS as the vehicles for T. gondii ribosome protein (TgRPS2) delivery. TgRPS2-PLGA and TgRPS2-CS nanospheres were synthesized by double emulsion solvent evaporation and ionic gelation technique as the nano vaccines. Before immunization in animals, the release efficacy and toxicity of the synthesized nanospheres were evaluated in vitro. Then, ICR mice were immunized intramuscularly, and immune protections of the synthesized nanospheres were assessed. The results showed that TgRPS2-PLGA and TgRPS2-CS nanospheres could induce higher levels of IgG and cytokines, activate dendritic cells, and promote the expression of histocompatibility complexes. The splenic lymphocyte proliferation and the enhancement in the proportion of CD4+ and CD8+ T lymphocytes were also observed in immunized animals. In addition, two types of nanospheres could significantly inhabit the replications of T. gondii in cardiac muscles and spleen tissues. All these obtained results in this study demonstrated that the TgRPS2 protein delivered by PLGA or CS nanospheres provided satisfactory immunoprotective effects in resisting T. gondii, and such formulations illustrated potential as prospective preventive agents for toxoplasmosis.
研究领域:
药剂学
·
免疫学
·
寄生虫学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Shiqi Wang,Li Wang,Yongmei Zhu,Yonghai Song
The detection of S 2− is of great significance because excess S 2− can lead to a variety of serious physiological diseases. Here, two metal-organic frameworks (MOFs), ZnMOF-74 and CuMOF-74, were synthesized by using 2,5-dihydroxy terephthalic acid with strong fluorescence as organic ligand and Zn 2+ or Cu 2+ as central coordination ions for S 2− detection. Both as-prepared ZnMOF-74 and CuMOF-74 displayed nanospheres with a diameter of about 100 nm. Under the excitation of 353 nm, the ZnMOF-74 had a characteristic emission peak at 537 nm and the CuMOF-74 had a characteristic emission peak at 528 nm under excitation of 356 nm. The interaction of S 2− and Zn 2+ weakened the fluorescence of ZnMOF-74 but the interaction of S 2− with Cu 2+ to form CuS restored the fluorescence of CuMOF-74, so the ZnMOF-74 and CuMOF-74 were exploited as a fluorescent nanosensor for sensing S 2− . The ZnMOF-74 sensor has a good linear range of 19.6 nmol L −1 -90.0 μmol L −1 , and the limit of detection was as low as 6.53 nmol L −1 . The CuMOF-74 sensor has a good linear relationship with I I 0 in the S 2− concentration range of 1.50 nmol L −1 -125 μmol L −1 , and the limit of detection was 1.50 nmol L −1 . The proposed ZnMOF-74 and CuMOF-74 sensor could also detect S 2− in actual samples.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）