Journal of Materials Chemistry C
·
2023
·
IF 6.4
Xiyue Bai,Ningyue Chen,Zhou Cao,Yuan Li
The urgency about the application of large-area molecular junction into aqueous environments has garnered significant attention, driven by the necessity for biomolecular testing under physiological conditions and the monitoring of chemical reactions in solutions. In this study, we synthesized a conductive ionogel as top electrode to measure the electrical properties of self-assembled monolayers (SAMs). The SAMs for test are with different terminal groups including -COOH, -CH3 and -F, exhibiting distinct affinities with water molecules. The variation in current density for each SAM can be attributed to the amount of water molecules between the electrode and SAMs. The electrical tests conducted in buffer solutions indicate that our tunneling process is dominated by electrons rather than ions, laying the foundation for testing biomolecules in physiological environments. We utilized ionogel electrodes for testing molecular junctions based on myoglobin and conducted temperature-dependent experiments with excellent repeatability. The exploration of ionogel electrodes contributes to our ability to conduct more complex measurements like rapid testing or drug screening, particularly in aqueous environments.
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高影响因子
Journal of Energy Chemistry
·
2025
·
IF 14.9
Chi Li,Yuheng Li,Zilong Zhang,Yingbing Zou,Jilei Wang,Shaojuan Bao,Siyi Jiang,Abd. Rashid bin Mohd Yusoff,Yaming Yu,Jing Ma,Peng Gao
Despite the exceptional efficiency of hole-selective self-assembled molecular layer (SAML), challenges persist due to SAML agglomeration and the incomplete passivation of buried perovskite defects, which hinder efficient hole transfer and compromise device stability. In this study, we introduce a novel V-shaped molecule, 4,4′-(perfluorocyclopent-1-ene-1,2-diyl)bis( N , N -bis(4-methoxyphenyl)aniline) (DPTAE), comprising a perfluorocyclopentene core flanked by two triphenylamine units, designed for incorporation at the perovskite buried interface. Owing to its sterically hindered, nonplanar structure, DPTAE functions as a “wedge” within the SAML, disrupting molecular aggregation and yielding an amorphous hole-selective layer. This redefined interfacial architecture facilitates enhanced charge extraction and minimizes interfacial defect states. As a result, the optimized wide-bandgap perovskite solar cells (PSCs) with a bandgap of 1.68 eV achieved an impressive power conversion efficiency (PCE) of 22.33 % and a fill factor ( FF ) of 86.24 %. Notably, the encapsulated devices exhibited superior stability under damp-heat conditions (ISOS-D-3, 85 % RH, 85 °C) with a T 88 of 1000 h, and maintained stable maximum power point tracking (ISOS-L-2, 40 % RH) with a T 92 of 500 h. Furthermore, DPTAE-based monolithic perovskite/silicon tandem solar cells attained a remarkable efficiency of 30.50 %. This innovative approach not only deepens our understanding of interfacial dynamics but also opens new avenues for engineering advanced solar cell architectures, thereby advancing perovskite-based photovoltaic technologies.
研究领域:
光伏技术
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材料科学
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太阳能
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界面工程
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有机电子学
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高影响因子
MOLECULES
·
2025
·Vol. 30
·期 6
·1299
·
IF 4.6
Fengming Guo,Xuteng Yu,Yuheng Li,Yong Chen,Chi Li,Chunming Liu,Peng Gao
The interface between nickel oxide (NiOx) and self-assembled monolayers (SAMs) in perovskite solar cells (PSCs) often suffers from limited adsorption strength, poor energy-level alignment, and inadequate defect passivation, which hinder device performance and stability. To address these issues, we introduce a hybrid hole selective layer (HSL) combining atomic layer deposition (ALD)-fabricated NiOxwith full-aromatic SAM molecules, creating a highly stable and efficient interface. ALD NiOx, enriched with hydroxyl groups, provides robust adsorption sites for the SAM molecule MeO-PhPACz, ensuring a strong, stable interaction. This hybrid HSL enhances energy-level alignment, hole selectivity, and defect passivation at the NiOx/perovskite interface. Devices utilizing this approach demonstrate significant performance improvements, achieving a power conversion efficiency (PCE) of 21.74%, with reduced voltage losses and minimal hysteresis. Furthermore, operational stability tests reveal enhanced durability under elevated humidity and temperature conditions. These findings highlight the potential of ALD NiOxand SAM hybrid HSL to overcome existing barriers, advancing the commercial viability of PSC technologies.
研究领域:
材料科学
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能源技术
·
光伏器件
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PubMed
高影响因子
Materials Today Energy
·
2025
·
IF 8.6
Zhangwei Yuan,Yichen Dou,Jiace Liang,Wenjuan Xiong,Meichen Liu,Yuanbo Song,Mengjun Liu,Tiancan Zhang,Ziyue Qiang,Yong Peng,Zhiliang Ku
Recent advancements in inverted perovskite solar cells have highlighted the role of self-assembled monolayer (SAM) hole transport layers, which offer thin profiles, ease of fabrication, and effective hole extraction, thereby enhancing solar cell efficiency. However, producing high-performance SAM films on rough substrates using traditional spin-coating is challenging due to the large, flat nature of typical SAM molecules, which leads to aggregation and insufficient film density. This can expose substrate areas to the perovskite layer, risking short circuits and compromising efficiency and stability. To overcome these challenges, we adopted a dip-coating adsorption method to prepare the SAM film while introducing chloromethylphosphonic acid (CPA) small molecules to occupy aggregation-induced vacancies. Our findings show that SAM molecules can successfully form dense films on rough FTO substrates using dip-coating, with CPA effectively filling gaps. Moreover, the chlorine groups in CPA enhance the vapor phase growth of the perovskite layer, reducing voids and promoting larger grain sizes. This dip-coating technique facilitated the development of a high-performance SAM/CPA composite hole transport layer, resulting in a perovskite solar cell with a power conversion efficiency of 21.37 %, which maintained 82.44 % efficiency after 1000 h of operation at maximum power.
研究领域:
材料科学
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能源技术
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光伏器件
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高影响因子
Advanced Science
·
2025
·
IF 14.1
Xiran Xiong,Jun Song,Shihan Li,Lu Jin,Qi He,Baohui Zhang,Yan Cao,Shanyong Yi,Yanfang Yang,Xiang Li,Juan Li,Wei Huang
O -Methylation catalyzed by plant O -methyltransferase plays a crucial role in both drug design and biosynthesis of natural products. However, their practical applications are often restricted by strict substrate specificity and a strong dependence on the expensive methyl donor S-adenosyl-L-methionine (SAM). Herein, an O -methyltransferase, SmOMT, is identified from the medicinal plant Selaginella moellendorffii , exhibiting substrate promiscuity and regioselectivity. SmOMT catalyzed the methylation of 25 structurally diverse substrates and demonstrated detectable N -methylation activity. Combined ternary complex structure and molecular dynamics studies of SmOMT elucidate its catalytic and regioselectivity mechanisms. A double mutant, SmOMT M2 , with enhanced catalytic activity is obtained based on structural analysis. To overcome SAM dependence, a cascade system for SAM regeneration is successfully constructed by coupling SmOMT M2 with a mutant halide methyltransferase, AtHMT V140T . Employing the iMARS platform, a highly active fusion enzyme, AtHMT V140T -L 95 -SmOMT M2 , is designed. This fusion enzyme outperforms the free-enzyme cascade system and facilitates the gram-scale synthesis of a series of methylated compounds with enhanced anti-inflammatory activity. This work provides a versatile methylating biocatalyst and establishes an efficient SAM regeneration methylation platform, overcoming limitations in enzymatic methylation and enabling the sustainable production of high-value pharmaceuticals.
研究领域:
酶学
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合成生物学
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生物催化
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结构生物学
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医药生物技术
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代谢工程
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PubMed
高影响因子
SENSORS
·
2017
·Vol. 17
·期 7
·1507
·
IF 2.677
Yuan Zhao,Qingrui Yang,Ye Chang,Wei Pang,Hao Zhang,Xuexin Duan
This paper demonstrates a novel micro-size (120 μm × 200 μm) piezoelectric gas sensor based on a piezotransduced single-crystal silicon bulk acoustic resonator (PSBAR). The PSBARs operate at 102 MHz and possess highQvalues (about 2000), ensuring the stability of the measurement. A corresponding gas sensor array is fabricated by integrating three different self-assembled monolayers (SAMs) modified PSBARs. The limit of detection (LOD) for ethanol vapor is demonstrated to be as low as 25 ppm with a sensitivity of about 1.5 Hz/ppm. Two sets of identification code bars based on the sensitivities and the adsorption energy constants are utilized to successfully discriminate isopropanol (IPA), ethanol, hexane and heptane vapors at low and high gas partial pressures, respectively. The proposed sensor array shows the potential to form a portable electronic nose system for volatile organic compound (VOC) differentiation.Keywords:e-nose system;piezotransduced single-crystal silicon bulk acoustic resonators;gas sensing;MEMS
研究领域:
微机电系统(MEMS)
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气体传感
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分析化学
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材料科学
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纳米技术
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DOI
PubMed
高影响因子
Cancer Immunology Research
·
2024
·
IF 8.1
Meng Meng,Zhong Zhaoyang,Song Liang,Zhang Zhaohui,Yin Xiaofeng,Xie Xiqiang,Tian Lei,Wu Wei,Yang Yao,Deng Yafei,Peng Hongyan,Wu Shuting,Ran Guanghe,Lin Yuqing,Lai Qiangqiang,Bi Qinghua,Yan Fulin,Ji Yan,Wang Yang,Li Xiaohui,Yi Ping,Yu Jianhua,Deng Youcai
Natural killer (NK) cells can be rapidly activated in response to cytokines during host defense against malignant cells or viral infection. However, it remains unclear what mechanisms precisely and rapidly regulate the expression of the numerous genes involved in activating NK cells. In this study, we discovered that NK-cell N6-methyladenosine (m6A) methylation levels were rapidly upregulated upon short-term NK-cell activation and were repressed in the tumor microenvironment. Deficiency of methyltransferase-like 3 (METTL3) or METTL14 moderately influenced NK-cell homeostasis, while double knockout of METTL3/14 significantly impacted NK-cell homeostasis, maturation, and antitumor immunity. This suggests a cooperative role of METTL3 and METTL14 in regulating NK-cell development and effector functions. Using methylated RNA immunoprecipitation sequencing (MeRIP-seq), we demonstrated that genes involved in NK-cell effector functions, such as Prf1 and Gzmb, were directly modified by m6A methylation. Furthermore, inhibiting mTOR complex 1 (mTORC1) activation prevented m6A methylation levels from increasing when NK cells were activated, and this could be restored by S-adenosylmethionine (SAM) supplementation. Collectively, we have unraveled crucial roles for rapid m6A mRNA methylation downstream of the mTORC1–SAM signal axis in regulating NK-cell activation and effector functions.
研究领域:
免疫学
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分子生物学
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癌症研究
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PubMed
高影响因子
Cell Death & Disease
·
2024
·Vol. 15
·期 10
·1-16
·
IF 8.1
Wan Xinyi,Zeng Weiwu,Fan Haonan,Wang Chenliang,Han Shixun,Sun Zhongxing,Tang Mei,Shao Juejia,Liu Yu,Fang Yuan,Jia Junqi,Tang Yin,Zhang Yanjun,Zhao Bin,Fang Dong
MAT2B works together with MAT2A to synthesize S-Adenosyl methionine (SAM) as the primary methyl donor. MAT2B, despite lacking catalytic activity, exerts regulatory control over the enzymatic activity of MAT2A. In addition to the enzymatic activity regulation, we find that, in an NADP + -dependent manner, MAT2B binds and stabilizes MAT2A. Disruption of the cellular NADP + remodels the protein level of MAT2A. The pentose phosphatase pathway regulates the level of MAT2A protein through the interaction of NADP + with MAT2B. Additionally, MAT2B-MAT2A interaction regulates the mRNA m6A modification and stability. In liver tumors, the Mat2a mRNA level is elevated but the protein level is decreased by the restricted NADP + . Blocking the interaction between MAT2B and MAT2A by the keto diet can suppress liver tumor growth. These findings reveal that MAT2B is essential for regulating the protein levels of MAT2A and connecting SAM synthesis to mRNA m6A.
研究领域:
癌症生物学
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分子生物学
·
生物化学
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PubMed
高影响因子
Emerging Microbes & Infections
·
2024
·
IF 8.4
Anke Chen,Ning Fang,Zhifei Zhang,Yiqing Wen,Yajie Shen,Yanjie Zhang,Lu Zhang,Guoping Zhao,Jin Ding,Jixi Li
The global outbreak of Mpox, caused by the monkeypox virus (MPXV), has attracted international attention and become another major infectious disease event after COVID-19. The mRNA cap N7 methyltransferase (RNMT) of MPXV methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs and plays a vital role in evading host antiviral immunity. MPXV RNMT is composed of the large subunit E1 and the small subunit E12. How E1 and E12 of MPXV assembly remains unclear. Here, we report the crystal structures of E12, the MTase domain of E1 with E12 (E1CTD-E12) complex, and the E1CTD-E12-SAM ternary complex, revealing the detailed conformations of critical residues and the structural changes upon E12 binding to E1. Functional studies suggest that E1CTD N-terminal extension (Asp545-Arg562) and the small subunit E12 play an essential role in the binding process of SAM. Structural comparison of the AlphaFold2-predicted E1, E1CTD-E12 complex, and the homologous D1-D12 complex of vaccinia virus (VACV) indicates an allosteric activating effect of E1 in MPXV. Our findings provide the structural basis for the MTase activity stimulation of the E1-E12 complex and suggest a potential interface for screening the anti-poxvirus inhibitors.
研究领域:
病毒学
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结构生物学
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分子生物学
·
传染病学
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2026
·
IF 19.9
Mengjiawei Liu,Wei Zhou,Jiayu Zheng,Zhuo Zhao,Zhijun Ning,Chaodan Pu
Aqueous processing of NiO x nanoparticle (NP) hole-selective layers suffers from a narrow precipitation window, poor colloidal stability, and poorly controlled surface chemistry that hampers the adsorption of phosphonic-acid self-assembled monolayers (SAMs). Here we introduce an inner-sphere nitrate surface-engineering strategy that converts trace Ni(NO 3 ) 2 , intentionally retained within Ni(OH) 2 precipitates, into strongly coordinated NO 3 − ligands during the Ni(OH) 2 -to-NiO x calcination. The resulting NiO x NPs form highly stable aqueous inks up to 40 mg mL −1 for >30 days and pass a 0.22 µm filter with ∼95% efficiency. By controlling base-addition kinetics during precipitation, we decouple particle size from nitrate coverage, tuning the surface NO 3 − /lattice-O ratio from 0.05 to 0.16 while maintaining an ∼7 nm NP size. The coordinated nitrate provides efficient anchoring sites for carbazole-based phosphonic-acid SAMs (e.g., MeO-2PACz), which increase effective SAM loading, enhance hole extraction, and suppress non-radiative recombination. Devices with the structure FTO/NiOx/SAM/perovskite/C 60 /BCP/Ag deliver a champion PCE of 26.1% (vs 24.4% for nitrate-poor NiO x ) and retain 80% of the initial efficiency after ∼1100 h MPP tracking at 65°C under encapsulation. This inner-sphere anion engineering establishes a simple, aqueous-compatible route to programmable NiO x surface chemistry, enabling reproducible ink processing and robust NiO x /SAM interfaces for high-efficiency inverted perovskite solar cells.
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高影响因子
IUBMB LIFE
·
2018
·Vol. 71
·期 3
·330-339
·
IF 3.236
Qing Wang,Maikun Teng,Xu Li
Catechol- O -methyltransferase (COMT 1 ) catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to various catechol substrates. COMTs play vital roles in physiological processes in animals, plants, and fungi, as well as bacteria, and have essential application values in industry. sp COMT is a probable COMT from Schizosaccharomyces pombe . It has an extraordinary intracellular distribution different from other homologs and would thus be predicted to perform a distinct physiological function. In this report, recombinant sp COMT was purified and kinetically characterized for the first time. The enzymology assays indicate that sp COMT is a metal-dependent enzyme and belongs to class I OMTs. In addition, the crystal structures of apo- sp COMT and SAM-complexed sp COMT were also presented, revealing that sp COMT possesses a conserved SAM-binding site and Mg 2+ pocket, but a distinct substrate pocket was not present in homologs. The mutagenesis ITC analysis revealed the SAM recognition characteristics of sp COMT. Based on all of the above findings, we speculated about the putative substrates’ characteristics and the substrate recognition mechanisms of sp COMT. This work will help in elucidating the physiological functions of sp COMT in S. pombe . © 2018 IUBMB Life, 71(3):330–339, 2019
研究领域:
生物化学
·
分子生物学
·
结构生物学
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PubMed
高影响因子
Science of Advanced Materials
·
2016
·Vol. 8
·期 5
·1066-1073
·
IF 1.812
Miao Jin-Lei,Liu Hai-Hui,Li Chuan-Bao,Zhang Xing-Xiang
Solution-processed graphene oxide (GO) films have tremendous potential in the applications of transparent electrodes. The mechanism of how the GO film is reduced by reducing gas has not been clarified because the reducing gas cannot permeate through the plane of GO. Here, an effective
chemical and thermal synergy reduction of GO film was investigated. We also revealed how the reducing gas permeates through the GO film barrier. The GO film was chemically reduced by the vapor of hydriodic acid and acetic acid (HI–AcOH, volume ratio of 1:1) at 100 °C for 3 h, followed
by annealing at 1100 °C for 0.5 h in an Ar atmosphere. The reduced graphene oxide (RGO) film had a sheet resistance of 562 Ω/〈 and a transparency of 63.7% at a typical wavelength of 550 nm, which is sufficient for many applications in optoelectronics, and this effective synergy
reduction method advances the research on graphene-based electrodes.
研究领域:
材料科学
·
纳米技术
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DOI
高影响因子
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