ACS Applied Nano Materials
·
2025
·
IF 5.5
Yiqun Su,Mian Gong,Yipeng Liu,Faiz Ullah Shah,Aatto Laaksonen,Rong An
Titanium dioxide (TiO2) nanostructures exhibit exceptional flexibility for integration into surface-enhanced Raman spectroscopy (SERS) sensing platforms enabling nanoscale trace detection of biomolecules with high sensitivity. However, fabricating TiO2 nanomaterials for large-scale SERS applications remains challenging due to the high cost and complexity of synthesis methods. In this study, we demonstrate a cost-effective and scalable approach using commercial TiO2 P25 nanoparticles as the SERS substrates, functionalized with a phosphonium-based fluorine-free ionic liquid (IL) comprising the trihexyl(tetradecyl)phosphonium cation ([P6,6,6,14]+) with four long alkyl chains and the 2–2-(2-methoxyethoxy)ethoxy anion ([MEEA]−). This IL serves as a nanoscale “linker”, effectively bridging Cytochrome c (Cyt c) molecules with TiO2 nanoparticles, significantly enhancing the Cyt c–TiO2 interactions and SERS signal intensity. The optimized system achieves remarkable sensitivity, enabling the detection of Cyt c concentrations as low as 5 × 10–4 M, with an enhancement factor increased by 1 order of magnitude compared to the control system. This work stresses the importance of nanoscale interactions and offers an alternative straightforward strategy for trace protein detection using commercially available TiO2 P25 nanoparticles, thus circumventing the need for complex nanomaterial fabrication methods.
研究领域:
生物传感
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纳米材料科学
·
分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoxiao Dong,Chang Lu,Shuaijie Guo,Siqi Zhang,Xuelian Hu,Wenliu Wang,Yiwei Tang,Shuo Wang,Xiuying Liu
A magnetic NiFe 2 O 4 @TiO 2 @Au composite was successfully synthesized via layer-by-layer assembly approach and exhibited excellent SERS performance. The composite was functionalized with an aptamer modified with Cy3, serving as the Raman signal reporter, and was hybridized with complementary DNA oligonucleotides (cDNA) to construct the NiFe 2 O 4 @TiO 2 @Au@ssDNA SERS aptasensor. This aptasensor enabled the development of a sensitive, specific, and straightforward SERS assay for acetamiprid detection, exhibiting good accuracy. Under optimized conditions, an inverse correlation was established between the acetamiprid concentration and the Raman intensity at the characteristic peak of 1132 cm −1 , spanning a range of 10 −1 -10 4 nmol/L with a LOD of 8.4 × 10 −1 pmol/L. Standard addition experiments to three types of leafy vegetables samples yielded recoveries ranging from 94.30 % to 111.83 %, with a relative standard deviation of <5.56 %. This method shows potential for detecting other hazardous substances in food and environmental samples by simply changing the corresponding aptamers and cDNA.
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Yifei Li,Yucui Zhang,Huan Jiang,Mengyao Qi,Xia Zhang,Baowang Zhu,Lei Han
Although the fact that H 2 O 2 is employed in the medical, food, and chemical industries, trace detection of H 2 O 2 remains a difficult and significant problem. For the detection of H 2 O 2 residues, SERS assays based on enzyme inhibition techniques show promise. As a result, we csynthesized CeO 2 @nanogel/Au nanoparticles with dual enzyme-like capabilities to detect hydrogen peroxide in this study. CeO 2 nanozymes possess both peroxidase-like and oxidase-like activities which could oxidize colorless 3,3′,5,5′-tetramethylbenzidine to blue TMB oxide. Surprisingly, after the addition of nanogel, CeO 2 @nanogel were prepared and both of its dual enzymatic activities decreased, especially the oxidase-like enzymes almost disappeared. To better investigate the enzyme catalytic activity, Au NPs were introduced and CeO 2 @nanogel/Au nanozymes were self-assembly prepared. Based on the significantly enhancement of CeO 2 @nanogel/Au nanozymes peroxidase activity, we developed a Raman detection method for H 2 O 2 with TMB ox as the probe molecule. The Raman intensity showed good linearity for the concentration of H 2 O 2 in the range of 10 -9 M−10 −1 M with a correlation coefficient of R 2 = 0.9922 and a detection limit of 2.68 × 10 -12 M. Thus, the proposed SERS mode sensor exhibited good selectivity and interference resistance, providing a new avenue for environmental monitoring.
研究领域:
生物化学
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分析化学
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环境科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Di Liu,Huimin Gao,Wenji Jiang,Su Yan,Hao Liu,Junjie Chen,Sisi Wen,Wei Zhang,Xu Wang,Bing Zhao,Wei Song
In this work, three-dimensional (3D) Ag aerogel-supported Hg single-atom catalysts (SACs) were explored as an efficient surface-enhanced Raman scattering (SERS) substrate to monitor the enhanced oxidase-like reaction. The influence of the concentrations of Hg2+ to prepare 3D Hg/Ag aerogel networks on their SERS properties to monitor the oxidase-like reaction has been investigated, and a specific enhancement with an optimized addition of Hg2+ has been achieved. The formation of Ag-supported Hg SACs with the optimized Hg2+ addition was identified from a high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM) image and X-ray photoelectron spectroscopy (XPS) measurement at an atomic level. This is the first discovery of Hg SACs for enzyme-like reaction applications inferred by SERS techniques. And density functional theory (DFT) was used to further reveal the oxidase-like catalytic mechanism of Hg/Ag SACs. This study provides a mild synthetic strategy to fabricate Ag aerogel-supported Hg single atoms to display promising prospects in various catalytic fields.
研究领域:
催化化学
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纳米生物学
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表面增强拉曼光谱学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Sasa Peng,Zhilong Zhang,Jialin Guo,Tianchen Ma,Dongli Liu
In this paper, we developed a simple, rapid and sensitive method for detection of thiram on apple surfaces by surface enhance Raman spectroscopy (SERS) combined with chemometric methods. Ag NCs (Ag nanocubes) were firstly prepared by a sulfide-mediated polyol method. Then the flexible and adhesive Ag NCs@PDMS substrates were obtained by combining Ag NCs self-assembled films with PDMS films. Thiram residues on apple surfaces were transferred to the substrate using adhesion properties of Ag NCs@PDMS. And the SERS spectra were obtained by Raman microscopy and analyzed with chemometric methods. The results were analyzed by principal component analysis (PCA), for the limit of detection (LOD) of thriam on apple surfaces was 0.01 ppm. Principal component regression (PCR) and partial least squares regression (PLSR) were explored to develop quantitative models. Both models represented higher correlation coefficients (close to 1), but PLSR models exhibited better predictive performance, with the correlation coefficient was 0.99282 with a low root mean squared error of calibration (RMSEC = 0.438) and root mean squared error of validation (RMSECV = 0.597). The developed SERS method based on Ag NCs@PDMS substrate provide a simpler and more sensitive way to monitor thiram on apple surfaces.
研究领域:
分析化学
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食品科学
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农残检测
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 11
·3649-3658
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IF 4.019
Qianqian Fang,Yingying Li,Xinxing Miao,Yiqiu Zhang,Jun Yan,Tainrong Yu,Jian Liu
Serious healthcare concerns have been raised on the issue of antibiotic residues after overuse, especially by accumulation in the human body through food webs. Here, we report a methodological development for sensitive detection of antibiotics with aptamer conformation cooperated enzyme-assisted SERS (ACCESS) technology. We design and integrate a set of nucleic acid oligos, realizing specific recognition of chloramphenicol (CAP) and efficient exonuclease III-assisted DNA amplification. It features a “signal-on” analysis of CAP with the limit of detection (15 fM), the lowest concentration detectable in the literature. Our method exhibits a high selectivity on the target analyte, free of interference of other potential antibiotic contaminants. The ACCESS assay promises an ultrasensitive and specific detection tool for trace amounts of antibiotic residues in samples of our daily life.
研究领域:
分析化学
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生物技术
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食品安全
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环境科学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2020
·
IF 0
Xinyuan Bi,Yuqing Gu,Jian Ye
Single-molecule (SM) detection in surface-enhanced Raman spectroscopy (SERS) demonstrates ultrahigh sensitivity and boosts wide applications. The bianalyte SERS (BiASERS) technique largely improves the accuracy and reliability of SM SERS detection over the monoanalyte approach by avoiding the use of ultralow-concentration samples and induced inaccuracy and very limited events. Herein, dual-type single-nanoparticle (SNP) SERS detection, an adapted version of the bianalyte SM technique, was developed for the first time with two types of ultrabright gap-enhanced resonance Raman tags (GERRTs) with embedded near-infrared resonant fluorescent molecules, showing concentration- and acquisition time-dependent behaviors and the SNP sensitivity in both aqueous and dry states. Additionally, a correlative Raman imaging and scanning electron (RISE) microscopy confirms the SNP events of GERRTs on the substrate. Therefore, we expand the feasible application scenarios of the BiASERS technique from merely SM to SNP detection and more importantly demonstrate the effectiveness of the pure statistical model of BiASERS with the undoubted experimental examinations. Dual-type SNP detection of GERRTs may open new opportunities for development of ultrasensitive nanotags for reliable localized and quantitative detection in biological environments.
研究领域:
纳米生物学
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生物医学工程
·
光谱学
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产品关联
DOI
Jove-Journal of Visualized Experiments
·
2023
·
IF 1.2
Ying Zheng,Xia Xiao,Zhenzhen Li,Yong Shao,Jieyi Chen,Zhouyi Guo,Huiqing Zhong,Zhiming Liu
Surface-enhanced Raman scattering (SERS) technology has attracted more and more attention in the biomedical field due to its ability to provide molecular fingerprint information of biological samples, as well as its potential in single-cell analysis. This work aims to establish a simple strategy for label-free SERS bioanalysis based on Au@carbon dot nanoprobes (Au@CDs). Here, polyphenol-derived CDs are utilized as the reductant to rapidly synthesize core-shell Au@CD nanostructures, which allows powerful SERS performance even when the concentration of methylene blue (MB) is as low as 10-9 M, due to the cooperative Raman enhancement mechanism. For bioanalysis, Au@CDs can serve as a unique SERS nanosensor to identify the cellular components of biosamples (e.g., cancer cells and bacteria). The molecular fingerprints from different species can be further distinguished after combination with the principal component analysis. In addition, Au@CDs also enable label-free SERS imaging to analyze intracellular composition profiles. This strategy offers a feasible, label-free SERS bioanalysis, opening up a new prospect for nanodiagnosis.
研究领域:
生物医学工程
·
纳米技术
·
分析化学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）