Sensor Letters
·
2017
·Vol. 15
·期 8
·639-646
·
IF 0
Yang Yu Jun,Li Weikun
In this work, multiwalled carbon nanotubes (MWCNT) were facilely functionalized with copper(II)/1Hbenzotriazole (BTA) complex (Cu-BTA) via π–π interaction. Cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were
used to characterize the Cu-BTA modified MWCNT, denoted as Cu-BTA/MWCNT. Experimental results indicate that Cu-BTA is redox-active and the surface of MWCNT was uniformly covered with the thin film of Cu-BTA. Further investigation shows that Cu-BTA/MWCNT exhibited excellent catalytic capabilities
towards the reduction of hydrogen peroxide. The sensors based on Cu-BTA/MWCNT modified glassy carbon electrodes (GCE), denoted as Cu-BTA/MWCNT/GCE, present wide linear range, low detection limit and high sensitivity for the determination of hydrogen peroxide. Using amperometric i–t
measurement, the linear range, the limit of detection (LOD) and the sensitivity for the detection of H2O2 are 5 × 10–7∼1.0 × 10–3 M, 2.21 × 10–7 M and 1.33 μA/μM, respectively.
Cu-BTA/MWCNT/GCE was also successfully used in the determination of hydrogen peroxide because of its excellent selectivity and stability.
研究领域:
材料科学
·
电化学
·
分析化学
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DOI
Sensor Letters
·
2016
·Vol. 14
·期 11
·1161-1167
·
IF 0
Ibupoto Zafar Hussain,Tahira Aneela,Mallah Arfana Begum,Willander Magnus,Yu Cong
In this investigation, novel nanostructures of CuO are synthesized by hydrothermal method using biotemplates. The CuO nanomaterial was characterized by various analytical techniques such as scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy
(XPS). These techniques showed well controlled morphology, excellent crystalline quality, and high purity in chemical composition. The glassy carbon electrode was modified with these CuO nanostructures for the electrochemical determination of dopamine. The electrochemical response of modified
electrodes was measured by cyclic voltammetry and amperometric techniques. The CuO nanostructures had shown excellent catalytic activity towards the oxidation of dopamine. The presented dopamine biosensor exhibits wide linear range of dopamine concentrations, low limit of detection, sensitive,
selective and reproducible features. The linear range for the CuO nanostructures based dopamine biosensor falls in the range of 25 to 870 μM and limit of detection was found 0.1 μM. There was found negligible response for common interferents such as ascorbic acid, uric
acid, and glucose. Finally, the proposed dopamine biosensor was potentially applied for the quantification of dopamine from real samples and the obtained results are very satisfactory. This study provides the solid route for other applications of CuO based on its improved catalytic properties.
研究领域:
材料科学
·
分析化学
·
生物医学工程
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DOI
Sensor Letters
·
2014
·Vol. 12
·期 10
·1462-1468
·
IF 0.558
Liu Zhi-Min,Zheng Ying-Ying,Jing Yan-Feng,Li Jie,Zhan Hai-Jun,Shen Qi
A facile, one-step synthesis of ionic liquid-functionalized graphene (IL-GR) nanocomposite using graphene oxide (GO) and amine-terminated ionic liquid (NH2-IL) was first presented through an epoxide ring-opening reaction, then acetylcholinesterase (AChE) was immobilized onto
IL-GR nanocomposite film modified glassy carbon electrode to prepare a highly sensitive AChE biosensor. The morphologies and electrochemistry properties of the composite were investigated by using scanning electron microscopy, transmission electron microscopy and cyclic voltammetry, respectively.
The nanocomposite not only provided a biocompatible microenvironment to keep the bioactivity of AChE, but also exhibited a strong synergetic effect on improving the sensing properties of phoxim. The immobilized AChE showed favorable affinity to acetylthiocholine (ATCl) and could catalyze the
hydrolysis of ATCl with a Kapp
m
value of 0.143 mM. The proposed biosensor exhibited a detection limit of 6.0 × 10–11 M (S/N = 3) towards phoxim. Besides, the biosensor is easy to prepare, have good stability, and will have potential application
in organophosphate pesticides.
研究领域:
分析化学
·
环境科学
·
生物技术
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DOI
高影响因子
DEVELOPMENTAL CELL
·
2024
·
IF 10.7
Huiqin Wang,Liping Zhu,Mengyuan Fan,Shuangshuang Weng,Xin Zhou,Hanxuan Zhao,Yongcui Shen,Jiaquan Chai,Liyong Hou,Miaomiao Hao,Rezwan Tanvir,Ling Li,Guanghui Xiao
Strigolactone (SL) is a plant hormone required for plant development. DWARF53 (D53) functions as a transcription repressor in SL signaling. However, the role of D53 in cotton ( Gossypium hirsutum , Gh) fiber development remains unclear. Here, we identify that GhD53 suppresses fiber elongation by repressing transcription of GhFAD3 genes, which control linolenic acid (C18:3) biosynthesis. Mechanistically, GhD53 interacts with SL-related transcriptional activate factor (GhSLRF) to prevent its binding on Omega-3 fatty acid desaturase gene ( GhFAD3 ) promoters, thereby inhibiting GhFAD3 transcription. Upon SL exposure, GhD53 is degraded and leads to GhSLRF activation. This activation further promotes GhFAD3 transcription, C18:3 biosynthesis, and fiber elongation. Our findings identify the molecular mechanism of how SL controls cell elongation via D53 and offer potential strategies to improve cotton quality through SL application.
研究领域:
植物生理学
·
分子生物学
·
农学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yulong Zhang,Suo Zheng,Jingru Chen,Zhiliang Cheng,Fei Liu,Yuehua Jiao
Lactococcus lactis undergoing aerobic respiration can consume oxygen and form an anaerobic environment. Salmonella , as a facultative anaerobic foodborne pathogen, multiply better under aerobic conditions. The purpose of this study was to explore aerobic respiration of Lactococcus lactis subsp. lactis ( L. lactis ) KLDS 4.0325 inhibitory effect on Salmonella enterica subsp. enterica serovar Typhimurium ( S. Typhimurium) SL 1344 in vitro and its colonization ability in vivo . Results indicate that L. lactis KLDS 4.0325 could inhibit the growth of S. Typhimurium SL1344 by producing bacteriocin. And L. lactis KLDS 4.0325 undergoing aerobic respiration could enhance this inhibitory effect, while reducing the expression levels of virulence genes sipB , sipC , and sopE2 in S. Typhimurium SL1344. L. lactis KLDS 4.0325 could be successfully labeled by CFDA-SE fluorescent probe, and the labeling rate was more than 97%. Murine experiments showed that L. lactis KLDS 4.0325 could adhere and colonize in the cecum and colon. Thus, L. lactis KLDS 4.0325 is a promising probiotic strain for the potential inhibition of Salmonella infection via its oxygen-scavenging in host gut.
研究领域:
微生物学
·
食品安全
·
分子生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xunzhi Deng,Qinyao Li,Jing Lu,Yilin Liu,Haiou Li,Ze Liao,Gang Huang,Langtao Xiao,Zhoufei Luo
Strigolactones (SLs) are novel plant hormones that play crucial roles in regulating plant growth and development. The stereoisomers of SLs often exhibit distinct biological activities, making their chiral separation and analysis essential for understanding their regulatory roles in plant branching. This study focused on the chiral separation and characterization of SL stereoisomers, including GR24 (synthetic) and strigol (natural). A magnetic nanocomposite (Ni₃(HITP)₂@Fe₃O₄@rGO) was synthesized for SL extraction, combined with SFC-MS/MS for precise separation and quantification. The method exhibited excellent linearity (0.2–50 ng/mL), minimal matrix effects (1.64–2.17 %), and low detection limits (1.93–3.76 pg/mL). Recoveries ranged from 86.90 % to 106.37 % with RSDs of 2.56 %–13.54 %. Under nitrogen and phosphorus stress, only (+)-strigol was detected in the roots of Oryza sativa L., suggesting its regulatory role under stress conditions. This reliable method provides robust support for exploring the physiological roles of SL stereoisomers in plants, shedding light on their functions in stress response and development.
研究领域:
植物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT AND SOIL
·
2026
·
IF 4.8
Chen Ji,Wang Yazhuo,Fan Zhongdi,Wang Conghu,Zhang Zihan,Liu Yuting,He Yanhui,Wu Zhansheng
Introduction Plant growth-promoting rhizobacteria (PGPR) colonize the rhizosphere and enhance plant growth, stress tolerance, and soil properties. To better harness these benefits, this study investigates the synergistic interaction between two PGPR strains— Bacillus subtilis SL-44 and Klebsiella oxytoca RS-5—and evaluates their combined effects on rapeseed growth through integrated direct and indirect mechanisms. Methods The plant growth-promoting traits (e.g., phosphate solubilization, siderophore and indole-3-acetic acid (IAA) production) and biofilm-forming capacity of individual strains and their consortium were characterized in vitro. Subsequently, pot experiments were conducted to evaluate their effects on rapeseed growth parameters and rhizosphere soil quality. Finally, the rhizosphere microbial communities were analyzed via high-throughput sequencing on the Illumina NextSeq platform. Results The co-culture consortium (SR) demonstrated synergistic enhancements in key plant growth-promoting traits and biofilm stability in vitro. Pot experiments confirmed that SR treatment most effectively promoted rapeseed biomass accumulation, improved physiological status, and elevated antioxidant capacity. It also optimally enhanced rhizosphere soil nutrient availability, enzyme activities, and selectively reshaped the microbial community by enriching beneficial taxa and increasing ecological network complexity. Ultimately, plant growth promotion was achieved through an integrated mechanism combining direct stimulation and microbiome-mediated soil improvement. Conclusion In summary, this study demonstrates that functional complementarity between PGPR strains drives synergistic growth promotion through combined direct stimulation and rhizosphere microbiome engineering, offering a framework for developing effective microbial consortia for sustainable agriculture. Graphical
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DOI
高影响因子
Inorganic Chemistry Frontiers
·
2025
·
IF 6.4
Wenjun Gong,Yating Pang,Chenyu Wang,Wenming Wang,Hong-Fei Wang
The dinitrosyl iron complex (DNIC) is a ubiquitous functional brick often used as a vehicle for the NO radical and chelatable iron pool, with a key role in chemical and biomedical applications. In this work, four DNICs modified with different thiolate bridging ligands (–SL) were synthesized. These complexes were characterized by 1H-NMR, electrospray ionization mass spectrometry, and X-ray crystal diffraction. Photoinduced NO release from the complexes in solution was further explored by time-resolved infrared and time-resolved electron paramagnetic resonance (EPR) spectra. The cluster complexes showed relatively lower cytotoxicity against the normal human liver cell line HL-7702 and higher cytotoxicity against human cervical cancer cells, with a selectivity index of 2.4–4.8. The cluster complexes with different L groups had different rates of NO release and protein binding constants. Moreover, NO released upon photoirradiation entered living cells, which were successfully imaged using an NO-selective fluorescent probe. The ferritin complex adducts further enhanced the cytotoxicity and cellular fluorescent intensity of NO, and the crystal structure revealed the coordination of the Fe(SL)2 unit with Cys102 of ferritin. This study provides insight into the photodynamic mechanism of nitrosyl iron−sulfur clusters to understand their physiological activity.
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产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2020
·
IF 2.455
Ting Zhang,Mengzhen Ding,Xichang Wang,Jian Zhong
The droplet and creaming stability of food emulsions stabilized by mixed emulsifiers is a research hotspot in the field of emulsions. In this work, we mainly explore the effect of pH change on the droplet and creaming stability of fish oil-loaded emulsions synergetically (Span 80 and SL) or competitively (Tween 80 and SDS) stabilized by gelatin/surfactant. The results demonstrate that initial droplet stability and droplet storage stability, and creaming stability of the pH-adjusted emulsions are dependent on both the adsorption ways of emulsifiers and the adjusted pH: (1) Competitively stabilized emulsions have more stable droplets than synergetically stabilized emulsions; (2) SDS-dominant competitively stabilized emulsions have more stable droplets than gelatin-dominant emulsions; (3) Basic pH-adjusted emulsions have more stable droplets than acidic pH-adjusted emulsions; (4) The synergetically stabilized emulsions at acidic pH have significantly higher creaming indexes than that at basic pH; and (5) The competitively stabilized emulsions have high or similar creaming indexes to that at acidic pH. Further, the mechanisms are proposed according to Stokes’ Law. This work will provide useful information to understand the interfacial properties of mixed emulsifiers in the food emulsions and promising application perspective for the development of food beverages including acidic and alkali beverages.
研究领域:
食品科学
·
化学工程
·
胶体科学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2020
·Vol. 30
·期 25
·2001196
·
IF 16.836
Liang Cheng,Zhengwei Cai,Tingjun Ye,Xiaohua Yu,Zhijie Chen,Yufei Yan,Jin Qi,Lei Wang,Zhihong Liu,Wenguo Cui,Lianfu Deng
Protein is the key composition of all tissues, which has also been widely used in tissue engineering due to its superior biocompatibility and low immunogenicity. However, natural protein usually lacks active functions such as vascularization, osteo-induction, and neural differentiation, which limits its further applications as a functional biomaterial. Here, based on the mimetic extracellular matrix feature of bovine serum albumin, injectable polypeptide-protein hydrogels with vascularization and antibacterial abilities are constructed successfully via coordinative cross-linking of sulfydryl groups with silver ions (Ag+) for the regeneration of infected wound. In this protein hydrogel system, (Ag+), acting as crosslinkers, can not only provide a sterile microenvironment and a strong and robust antibacterial ability but also introduce K 2 (SL) 6 K 2 (KK) polypeptide, which endows the hydrogel with vascularization behavior. Furthermore, the in vivo data show that the polypeptide-protein hydrogel has a considerable collagen deposition and vascularization abilities in the early stage of wound healing, resulting in rapid new tissue regeneration featured with newly appeared hair follicles. Altogether, this newly developed multifunctional 3D polypeptide-protein hydrogel with vascularization, antibacterial properties, and hair follicle promotion can be a promising approach in biomedical fields such as infected wound healing.
研究领域:
组织工程
·
生物材料
·
伤口愈合
·
生物医学工程
✓ 已验证
DOI
高影响因子
PARTICULATE SCIENCE AND TECHNOLOGY
·
2023
·
IF 2.5
Haifeng Liao,Haitao Yan,Yukun Wei,Xianbin Hou,Jinhong Du,Zibin Yin,Yongjian Wang,Leyang Dai
To improve the lipophilicity of ZnO nanopowder in lubricating oil and analyze the surface modification mechanism, stearic acid, oleic acid, and HTAB-SL were selected as surface modifiers. ZnO nanopowders were prepared and modified in a single step by dielectric barrier discharge plasma assisted ball milling for 10 h. The ZnO nanopowder was characterized and analyzed by SEM, FTIR, TG-DSC, x-ray diffractometer, x-ray photoelectron spectroscopy, lipophilicity determination, COF test, etc. The results showed that the size of ZnO nanopowder was 40–100 nm. The results also revealed that the lipophilic groups –CH2 and –CH3 of modifier were successfully modified by ZnO nanopowder. Meanwhile, hydroxyl groups –OH of ZnO nanopowder chemically adsorbed with hydrophilic carboxyl groups –COO– of modifier. The lipophilicity of modified ZnO nanopowder was significantly increased. The COF of oil sample with modified ZnO nanopowder was smaller than that of oil sample with unmodified ZnO nanopowder. The modification mechanism was attributed to the coupling action of plasma, modifier, and mechanical forces of ball milling. The research demonstrated that modified ZnO nanopowder is more suitable as lubricating additive.
研究领域:
材料科学
·
纳米技术
·
表面化学
·
机械工程
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Sustainability
·
2024
·
IF 7.1
Anhui Yu,Shiping He,Feiyan Fu,Zhiguang Guo
Ice accumulation on the surface of aerospace and transportation equipment under extreme weather seriously endangers the safety and efficiency of equipment operation. In recent years, metal corrosion, especially in the marine engineering industry, has become a global concern. The slippery liquid infused porous surface (SLIPS) is a new bionic surface which has caused extensive research in recent years, and has great potential in anti-corrosion and anti-icing. In this study, a multifunctional solid-like lubricated slippery surface (SL-SLIPS) was developed by superhydrophobic modification of the base of copper hydroxide nanoneedles and injection of solid-like lubricant synthesized by embedding oil-stored mesoporous silica nanoparticles on the surface of epoxy resin. At −30 °C, the delayed icing time on the surface of the SL-SLIPS sample was 22.6 times that of the original copper substrate. The ice adhesion was tested on the surface of the sample, and the results showed that the adhesion of the icicle on the SL-SLIPS surface was the smallest, which decreased by 89% compared with the original copper substrate. In addition, SL-SLIPS have excellent corrosion resistance and rapid droplet trapping, nucleation and drop. It has good practical application prospects in corrosion resistance of marine equipment and icing resistance of aerospace and transportation equipment. This study provides new ideas for the design of new slippery surfaces and their application in practical fields, such as anti-corrosion of offshore equipment, anti-icing of equipment in aviation and fog collection engineering in industry.
研究领域:
材料科学
·
表面工程
·
腐蚀科学
·
航空航天工程
·
海洋工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）