SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Ning Xu,Qinglong Qiao,Yi Tao,Pengjun Bao,Yinchan Zhang,Wei Zhou,Jin Li,Zhifeng Li,Zhaochao Xu
Subcellular pH plays a key role to modulate protein structures or specific active sites to impact diverse biological processes. The heterogeneity and high dynamics of intracellular pH require probes that can detect pH in the vicinity of the protein and have a wide pH response range. Here, we reported a protein proximity ligation fluorescent probe for super-resolution imaging of subcellular pH dynamics. Relying on SNAP-tag protein labeling technology, the pH-sensitive fluorophore BG-NDM based on photo-induced electron transfer mechanism was localized to the adjacent positions of the target protein in different subcellular organelles. The equilibrium between the non-fluorescent aggregate and the fluorogenic monomer enabled BG-NDM to have the ability of wash-free super-resolution imaging in live cells. And the wide pH response ranges from pH 6 to pH 12 made the probe have the ability of recognizing pH dynamics. The pH changes around functional proteins during mitophagy, cellular alkalosis and acidosis were revealed. Through long-term super-resolution imaging, we could further record the pH dynamics around mitochondrial proteins COX8A during mitochondrial contacts, fusion and fission at the resolution of a single mitochondria.
研究领域:
生物化学
·
细胞生物学
·
分子影像学
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-9
·
IF 5.3
Zhou Xinxin,Qi Min,Yang Minghui
The detection of botulinum neurotoxin A (BoNT/A) endopeptidase activity by pregnancy test paper based on human chorionic gonadotropin (hCG)-functionalized peptide-modified magnetic nanoparticles (MNs) is described for the first time. HCG-functionalized SNAP-25 peptide substrate with hydrolysis recognition sites was optimally designed. HCG can be recognized by pregnancy test strips. BoNT/A light chain (BoNT-LcA) is the central part of the endopeptidase function in holotoxin, which can specifically hydrolyze SNAP-25 peptide to release the hCG-peptide probe, and the hCG-peptide probe released can be quantitatively detected by pregnancy test strips, achieving indirect determination of BoNT/A. By quantifying the T-line color intensity of test strips, the visual detection limit for BoNT-LcA is 12.5 pg/mL, and the linear range of detection for BoNT-LcA and BoNT/A holotoxin was 100 pg/mL to 1 ng/mL and 25 to 250 ng/mL. The ability of the method to quantify BoNT/A was validated in human serum samples. This method shows the potential for sensitive detecting BoNT/A and has prospects for the diagnosis and prognosis of clinical botulism.
研究领域:
生物化学
·
医学诊断
·
纳米技术
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jie Chen,Hui Zhang,Yuxin Lin,Jinyu Liu,Yisong Chen,Xiaoyan Zhang,Hetong Lin,Da-Peng Yang
Food preservation technologies that combine antimicrobial activity with controlled-release functionality are essential to mitigate postharvest losses. Herein, chitosan (CS)-based coatings incorporating S-nitroso- N -acetyl-DL-penicillamine (SNAP) as a nitric oxide (NO) donor were developed for sustained antimicrobial release and fruit preservation. The CS/SNAP coatings showed high transparency (79.5–79.9 %), strong UV-blocking (T 350 nm < 1 %), and rapid water solubility, with flexibility increasing alongside SNAP loading. The coatings released NO over 15 days, exhibiting potent antibacterial effects against E. coli (24.64 mm inhibition zone), S. aureus (28.86 mm), and antifungal activity against B. cinerea (20.20 mm). When applied to tomatoes, the CS/SNAP12 coating reduces weight loss, maintains fruit firmness and inhibits ethylene production, thereby extending shelf life to 15 days. Cytotoxicity and seed germination tests confirmed biocompatibility. This scalable, eco-friendly solution synergistically controls respiration, microbial growth, and moisture loss, offering a promising approach to perishable food preservation.
研究领域:
食品科学与技术
·
材料科学
·
采后生物学
·
抗菌材料
·
可持续包装
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Zhang Quan,Jiang Yuhan,Chen Wenhao,Wang Jinjin,Zhang Xinchi,Wang Yuan,Xu Zhuoyan,Dong Hengtao,Han Shengli
In this study, a novel cell membrane chromatography (CMC) model was developed to discover Histamine H1 receptors (H 1 R) targeted anti-allergic drugs for specific screening and ligand-receptor interaction analysis by SNAP-tagged H 1 R fusion protein conjugation and BG-modified silica gel. Allergic diseases (ADs) are prevalent pathological conditions arising from immune dysregulation, which significantly compromise patient quality of life across multiple domains, while traditional Chinese medicines (TCMs) are widely used to treat ADs. H 1 R plays a pivotal role in the pathogenesis of allergic diseases and acts as an attractive target for therapeutic intervention. Although antihistamines are the first-line therapy for ADs, their clinical utility is limited by side effects. Consequently, a novel screening method for active components targeting H 1 R in TCMs is crucial for the research and discovery of novel anti-allergic lead compounds. In the current study, the H 1 R-SNAP-tag cell membrane chromatography (H 1 R-SNAP-tag/CMC) column was prepared with outstanding specificity, selectivity, and reproducibility by chemically immobilizing H 1 R overexpression cell membranes to the BG-silica gel (BG-SiO 2 ) using the SNAP-tag technology. The H 1 R-SNAP-tag/CMC-HPLC-MS system was investigated using the H 1 R antagonist Clemastine fumarate and the results showed that the system was a reliable model for screening potential anti-allergic compounds from TCMs. From an Iris tectorum Maxim. ( I. tectorum ) extract, six H 1 R-binding compounds were identified: tectoridin, iristectorin A, iridin, tectorigenin, irigenin, and irisflorentin. Frontal analysis and molecular docking confirmed their affinity for H 1 R, while subsequent in vivo and in vitro pharmacological assays preliminarily validated their anti-allergic efficacy. Graphical abstract
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EMBO JOURNAL
·
2020
·Vol. 39
·期 16
·e103631
·
IF 9.889
Xianping Wang,Jihong Gong,Le Zhu,Shen Wang,Xiaoyu Yang,Yuanyuan Xu,Xiaofei Yang,Cong Ma
Priming of synaptic vesicles involves Munc13-catalyzed transition of the Munc18-1/syntaxin-1 complex to the SNARE complex in the presence of SNAP-25 and synaptobrevin-2; Munc13 drives opening of syntaxin-1 via the MUN domain while Munc18-1 primes SNARE assembly via domain 3a. However, the underlying mechanism remains unclear. In this study, we have identified a number of residues in domain 3a of Munc18-1 that are crucial for Munc13 and Munc18-1 actions in SNARE complex assembly and synaptic vesicle priming. Our results showed that two residues (Q301/K308) at the side of domain 3a mediate the interaction between the Munc18-1/syntaxin-1 complex and the MUN domain. This interaction enables the MUN domain to drive the opening of syntaxin-1 linker region, thereby leading to the extension of domain 3a and promoting synaptobrevin-2 binding. In addition, we identified two residues (K332/K333) at the bottom of domain 3a that mediate the interaction between Munc18-1 and the SNARE motif of syntaxin-1. This interaction ensures Munc18-1 to persistently associate with syntaxin-1 during the conformational change of syntaxin-1 from closed to open, which reinforces the role of Munc18-1 in templating SNARE assembly. Taken together, our data suggest a mechanism by which Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.
研究领域:
神经科学
·
细胞生物学
·
分子生物学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Mengqi Han,Weikang Tang,Qinfei Chen,Hong Zhou,Jiadong Chen,Wenbin Liu
Amino acid surfactants have gained significant importance in overcoming the limitations of conventional surfactants, notably, their low biocompatibility and biodegradability. However, the current amino acid surfactants lack multifunctional properties due to the nonreactivity of their aliphatic chains, necessitating the development of a new type of amino acid surfactant. A novel melanin-like amino acid surfactant and a biomimetic synthesis route were devised by mimicking the biosynthesis of melanin. Renewable natural polyphenol compounds with catechol moieties were utilized as building blocks for the hydrophobic group. In a proof-of-concept experiment, ethyl protocatechuate was oxidized to o-quinone and subsequently covalently linked to the amino group of lysine via Michael addition. The chemical structure was verified using liquid chromatography–tandem mass spectroscopy. The melanin-like amino acid surfactant exhibited excellent surface-active properties, with a critical micelle concentration of 1.59 mN m–1. Furthermore, it demonstrated remarkable emulsifying, foaming, solubilizing, dispersing, and wetting capabilities. Notably, it also possessed multifunctionality, including antibacterial activity, antioxidant activity, robustness, and mildness. These outstanding properties indicate significant potential for various applications. This strategy offers innovative insights and a versatile, modular toolbox for synthesizing multifunctional amino acid surfactants that mimic melanin. The approach allows for the easy interchange of o-quinone building blocks, which is akin to snap jewelry.
研究领域:
生物化学
·
材料科学
·
有机化学
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PubMed
高影响因子
Advanced Science
·
2026
·
IF 14.1
Xin Li,Miao Xu,Heran Li,Huan Jiang,Xin Wang,Lianjun Ma,Ying-Wei Yang
Oral treatment of inflammatory bowel disease (IBD) faces significant challenges of poor targeting, low bioavailability, and systemic toxicity, necessitating intelligent stimuli-responsive delivery systems. Here, we design and synthesize a pH/hypoxia dual-responsive pendant azobenzene-functionalized MOF (Azo-MOF). The uniformly distributed azobenzene pendants serve as intrinsic gating sites, forming “snap-top” encapsulation with β-cyclodextrin through host-guest interactions that prevent premature 6-mercaptopurine leakage while enabling rapid stimuli-triggered release, creating a postmodification-free supramolecular gated delivery nanosystem (CAMM). CAMM exhibits hypoxia-triggered burst release and alkaline-sustained release, with <5.0% drug leakage during gastrointestinal transit and >90.0% cumulative release at inflammatory sites. Notably, the Azo-MOF carrier scavenges reactive oxygen species through synergy between its conjugated π -system and metal coordination environment, providing antioxidant protection alongside drug delivery. In dextran sulfate sodium-induced colitis models, CAMM outperformed free drug, achieving near-complete weight recovery, downregulating proinflammatory cytokines, restoring intestinal barrier integrity, and rebalancing gut microbiota by enriching Lactobacillus and Bifidobacterium while suppressing opportunistic pathogens. This study integrates targeted delivery, antioxidant therapy, and microbiota modulation, offering an effective strategy for precision IBD treatment.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yulin Mao,Tieqiang Sun,Tingting Gong,Kaiyuan Chen,Hao Zheng,Wenjia Niu,Jian Hou,Zhilong Lu,Chen Luo,Yuxuan Fu,Ran Zhang,Zhanxin Yao,Jialei Bai
The preparation of nanoparticle–nanobody (Nb) conjugates with high stability and affinity remains challenging in lateral flow immunoassay (LFIA) development. Herein, we employed a SNAP-O(6)-benzylguanine (BG) system to achieve site-specific conjugation of Nb-snap fusion proteins with BG-coated fluorescent microspheres (FMs), yielding FM/BG@Nb-snap probes with enhanced stability and bioactivity. The FM/BG@Nb-snap-LFIA enabled quantitative ochratoxin A (OTA) detection within 10 min, with a limit of detection of 0.06 ng/mL, representing 3.5-fold and 39.17-fold improvements over conventional covalent coupling and colloidal gold methods, respectively. Recovery rates for OTA detection in food samples ranged from 93.08% to 111.55%. Overall, this study offers a promising strategy for the design and preparation of Nb-based probes to enhance LFIA performance.
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高影响因子
AGRICULTURAL RESEARCH
·
2024
·Vol. 13
·期 2
·198-203
·
IF 1.4
Lv Na,Li Li,Wang Na,Guo Chang-jie,Zhang Hao-yan
Snap bean ( Phaseolus vulgaris L.) is very sensitive to low temperature during postharvest storage. Methyl jasmonate (MeJA) is a volatile hormone ubiquitous in many kinds of plants and plays important role in regulating plant physiological functions. The alleviating effect of MeJA treatment on chilling injury (CI) in postharvest snap bean was studied. We found MeJA treatment markedly decreased the index of chilling injury, electrolyte leakage and content of malondialdehyde. MeJA-treated snap bean exhibited higher levels of total soluble solids and titratable acidity. Similarly, MeJA could prevent the degradation of chlorophyll and ascorbic acid. Not only total antioxidant capacity but also metal chelating ability of MeJA-treated snap bean was stronger than untreated control. MeJA-treated snap bean showed higher scavenging activities on superoxide radical (O 2 .- ), hydroxyl radical (·OH) and 1,1-diphenyl-2-trinitrophenylhydrazine radical either. MeJA application stimulated the activities of ascorbate peroxidase (APX), peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) in snap bean. In conclusion, this study reveals MeJA can be used to relieve chilling injury and maintain the quality of snap bean during low-temperature storage. 8 µM MeJA is the optimal concentration to alleviate chilling injury of snap bean.
研究领域:
植物学
·
植物生理学
·
采后生物学
·
食品科学
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DOI
高影响因子
Chemical Science
·
2023
·Vol. 15
·期 3
·1018-1026
·
IF 8.4
Zi-Sheng Li,Wei-Xing Chen,Harry W. T. Morgan,Cong-Cong Shu,John E. McGrady,Zhong-Ming Sun
The endohedral Zintl-ion cluster [Fe3Sn18]4− contains a linear Fe3 core with short Fe–Fe bond lengths of 2.4300(9) Å. The ground state is a septet, with significant σ and π contributions to the Fe–Fe bonds. The Sn18 cage is made up of two partially fused Sn9 fragments, and is structurally intermediate between [Ni2CdSn18]6−, where the fragments are clearly separated and [Pd2Sn18]4−, where they are completely fused. It therefore represents an intermediate stage in cluster growth. Analysis of the electronic structure suggests that the presence of the linear Fe–Fe–Fe unit is an important factor in directing reactions towards fusion of the two Sn9 units rather than the alternative of oligomerization via exo bond formation.
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高影响因子
Cell Reports
·
2023
·Vol. 42
·期 11
·113349-113349
·
IF 8.8
Shao Q,Wijaya CS,Wang S,Meng X,Yuan C,Ma C,Xu S
Maintaining cellular viability relies on the integrity of the plasma membrane, which must be repaired upon damage. Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-mediated membrane fusion is a crucial mechanism involved in membrane repair. In C. elegans epidermal cell hyp 7, syntaxin-2 (SYX-2) facilitates large membrane wound repair; however, the underlying molecular mechanism remains unclear. Here, we found that SNAP-25 protein RIC-4 and synaptobrevin protein SEC-22 are required for SYX-2 recruitment at the wound site. They interact to form a SNARE complex to promote membrane repair in vivo and fusion in vitro. Moreover, we found that SEC-22 localized in multiple intracellular compartments, including endosomes and the trans-Golgi network, which recruited to the wounds. Furthermore, inhibition of RAB-5 disrupted SEC-22 localization and prevented its interaction with SYX-2. Our findings suggest that RAB-5 facilitates the formation of the RIC-4/SEC-22/SYX-2 SNARE complex and provides valuable insights into the molecular mechanism of how cells repair large membrane wounds.
研究领域:
细胞生物学
·
分子生物学
·
发育生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Guangbin Zheng,Rulin Li,Peixuan Wu,Lei Zhang,Yao Qin,Shungang Wan,Jie Pei,Peng Yu,Kun Fu,Mark E. Meyerhoff,Yuanyuan Liu,Yang Zhou
Currently, the controlled release of nitric oxide (NO) plays a crucial role in various biomedical applications. However, injectable NO-releasing materials remain an underexplored research field to date. In this study, via the incorporation of S -nitroso- N -acetyl-penicillamine (SNAP) as an NO donor, a family of NO-releasing injectable hydrogels was synthesized through the in situ cross-linking between sodium alginate and calcium ion induced by D-(+)-gluconate δ-lactone as an initiator. Initially, the organic functional groups and the corresponding morphologies of the resulting injectable hydrogels were characterized by IR and SEM spectroscopies, respectively. The NO release times of hydrogels with different SNAP loading amounts could reach up to 36–47 h. Due to the release of NO, the highest antibacterial rates of these injectable hydrogels against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) were up to 95 %, respectively. Furthermore, the matrix of these hydrogels demonstrated great water absorption ability, swelling behavior, and degradation performance. Finally, we expect that these NO-releasing injectable hydrogels could have great potential applications various biomedical material fields.
研究领域:
材料科学
·
药学
·
生物医学工程
·
化学工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）