ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2017
·
IF 3.743
Xiong Pan,Saige Wang,Nan Shi,Hua Fang,Yunlong Yu
The application of the herbicide chlorimuron-ethyl has a lasting toxic effect on some succession crops. Here, a bacterium capable of utilizing chlorimuron-ethyl as the sole source of nitrogen was isolated from the contaminated soil and was identified as Enterobacter ludwigii sp. CE-1, and its detoxification and degradation of the herbicide were then examined. The biodegradation of chlorimuron-ethyl by the isolate CE-1 was significantly accelerated with increasing concentration (1–10 mg/l) and temperature (20–40 °C). The optimal pH for the degradation of chlorimuron-ethyl by the isolate CE-1 was pH 7.0. A pathway for the biodegradation of chlorimuron-ethyl by the isolate CE-1 was proposed, in which it could be first converted into 2-amino-4-chloro-6-methoxypyrimidine and an intermediate product by the cleavage of the sulfonylurea bridge and then transformed into saccharin via hydrolysis and amidation. The plant height and fresh weight of corn that had been incubated in nutrient solution containing 0.2 mg/l of chlorimuron-ethyl significantly recovered to 83.9% and 83.1% compared with those in the uninoculated control, although the root growth inhibition of chlorimuron-ethyl could not be alleviated after inoculation for 14 d. The results indicate that the isolate CE-1 is a promising bacterial resource for the biodegradation and detoxification of chlorimuron-ethyl.
研究领域:
环境科学
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微生物学
·
植物生理学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Qilu Cheng,Qifa Zhou,Zhuo Jin,Ying Jiang,Ligen Xu,Hui Jiang,Yuhua Zhao
Algal cultures of Dictyosphaerium sp. were treated with a wide range (0–8 mg/L) of nonylphenol (NP) applications to investigate the species' potential to perform bioremediation of NP-contaminated wastewater and explore the mechanism of NP toxicity in algal cell. The algal species exhibited a high tolerance for NP and efficiently removed even high concentrations of NP. The accumulation of NP in algal cells tended to increase and the percentage of NP adhered to the cell walls tended to decrease as the NP concentration increased. The thylakoid density and the pyrenoid size also tended to decrease as the NP concentration rose. The key genes involved in the antioxidative pathways and photosynthetic pathways were down-regulated in the NP-treated algal cells. Algal ribosome genes were especially sensitive to NP. The results indicated that NP could induce oxidative stress in algal cells and that Dictyosphaerium sp. showed potential as a bioremediation agent.
研究领域:
环境科学
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分子生物学
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DOI
PubMed
高影响因子
Gels
·
2024
·Vol. 10
·期 8
·502
·
IF 5
Xiong Li,Chuyi Li,Yizhou Liu,Gang Han,Congyu Lin,Xiaoli Chen,Jian Mao
Carrageenans were widely utilized as thickening and gelling agents in the food and cosmetic industries, and their oligosaccharides have been proven to possess enhanced physicochemical and biological properties. In this study,Shewanella sp.LE8 was utilized for the depolymerization of κ-, ι-, and λ-carrageenan under conditions of fermentation. During a 24-h fermentation at 28 °C, the apparent viscosity of κ-, ι-, and λ-carrageenan decreased by 53.12%, 84.10%, and 59.33%, respectively, accompanied by a decrease in storage modulus, and loss modulus. After a 72-h fermentation, the analysis of methylene blue and molecular weight distribution revealed that ι-carrageenan was extensively depolymerized into smaller polysaccharides byShewanella sp.LE8, while exhibiting partial degradation on κ- and λ-carrageenan. However, the impact ofShewanella sp.LE8 on total sugars was found to be limited; nevertheless, a significant increase in reduced sugar content was observed. The ESIMS analysis results revealed that the purified components obtained through ι-carrageenan fermentation for 72 h were identified as tetrasaccharides, while the two purified components derived from λ-carrageenan fermentation consisted of a hexasaccharide and a tetrasaccharide, respectively. Overall, the present study first reported the depolymerization of ι-and λ-carrageenan byShewanellaand suggested that theShewanellacould be used to depolymerize multiple carrageenans, as well as complex polysaccharides derived from red algae, to further obtain their oligosaccharides.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
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2014
·Vol. 37
·期 1
·183-188
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IF 1.736
He Yu-Cai,Tao Zhi-Cheng,Zhang Dan-Ping,Yang Zhen-Xing,Gao Shan,Ma Cui-Luan
Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. ( R )-1,2-Propanediol (>99 % e.e. ) was obtained at 44 % yield with growing cells. Glycol sulfide, ethylene sulfate, and 1,3-PDS were converted into the corresponding diols at 94.6, 96.3, and 98.3 %, respectively. Optimal reaction conditions with lyophilized resting cells were 30 °C, pH 7.5, and cell dosage 17.9 mg cell dry wt/ml. 1,3-Propanediol was obtained from 50 mM 1,3-PDS at 97.2 % yield by lyophilized cells after 16 h. Lyophilized cells were entrapped in calcium alginate with a half-life of 263 h at 30 °C, and the total operational time of the immobilized biocatalysts could reach over 192 h with a high conversion rate.
研究领域:
微生物学
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生物催化
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DOI
PubMed
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 5
·1171
·
IF 4.2
Shuyun Li,Jiazhen Liu,Ping Fang
The development of efficient bioremediation technologies for polycyclic aromatic hydrocarbons contamination is a hot research topic in the environmental field. In this study, we found that theMycobacteriumsp., TJFP1, has the function of degrading low molecular weight PAHs, and further investigated its degradation characteristics using the PAH model compound phenanthrene as a target pollutant. The optimal growth and degradation conditions were determined by single-factor experiments to be 37 °C, pH 9.0, and an initial concentration of 100 mg/L phenanthrene. Under this condition, the degradation efficiency of phenanthrene reached 100% after 106 h of incubation, and the average degradation rate could reach 24.48 mg/L/day. Combined with whole genome sequencing analysis, it was revealed that its genome carries a more complete phenanthrene degradation pathway, including functional gene clusters related to the metabolism of PAHs, such as phd and nid. Meanwhile, intermediates such as phthalic acid were detected; it was determined that TJFP1 metabolizes phenanthrene via the phthalic acid pathway. Simulated contaminated soil experiments were also conducted, and the results showed that the removal rate of phenanthrene from the soil after 20 days of inoculation with the bacterial strain was about 3.7 times higher than that of the control group (natural remediation). At the same time from the soil physical and chemical properties and soil microbial community structure of two levels to explore the changes in different means of remediation, indicating that it can be successfully colonized in the soil, and as a dominant group of bacteria to play the function of remediation, verifying the environmental remediation function of the strains, for the actual inter-soil remediation to provide theoretical evidence. This study provides efficient strain resources for the bioremediation of PAH contamination.
研究领域:
环境科学
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微生物学
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基因组学
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DOI
PubMed
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2025
·
IF 7.8
Yafang Li,Jia Wei,Xiaofang Liang,Jiangping Wang,Sisi Yan,Lemei Zhu,Zhen Ding,Chunhua Zhan,Xiaoya Ren,Fei Yang
Microcystin-LR (MC-LR) is a widely distributed cyanotoxin that causes kidney injury. Our previous work identified Sphingopyxis sp. YF1 as a novel bacterial strain capable of efficiently degrading MC-LR, but its protective role and mechanisms against MC-LR-induced kidney injury remain unexplored. In this study, the bioactive metabolite of strain YF1 was identified by mass spectrometry. The beneficial effect of strain YF1 and its bioactive metabolite against MC-LR-induced kidney injury was validated in vivo and in vitro . We found strain YF1 effectively ameliorated MC-LR induced kidney disruption and functional impairment. Mechanistically, strain YF1 significantly improved oxidative stress (SOD, CAT, GSH, and MDA) and inhibited pro-inflammatory factors expression (including IL-6 , IL-18 , IL-1β , and TNF-α ) induced by MC-LR. Notably, strain YF1 not only reduce MC-LR level in mice but also produce a bioactive metabolite astaxanthin. Consistently, both in vivo and in vitro experiments demonstrated that astaxanthin alleviated MC-LR-induced kidney injury by modulating antioxidant pathways and inflammatory cascades. This study suggested that strain YF1 could alleviate MC-LR-induced kidney injury by removing MC-LR and generating astaxanthin that mediated antioxidant and anti-inflammatory effects. These findings highlighted microbial-based antidotes for simultaneously eliminating MC-LR and mitigating its harmful effects.
研究领域:
环境生物学
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毒理学
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微生物学
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DOI
高影响因子
PETROLEUM SCIENCE AND TECHNOLOGY
·
2019
·
IF 1.07
Shuiquan Chen,Shuo Sun,Chaocheng Zhao,Qiyou Liu,Meng Zang
The biodesulfurization capacity of Gordonia sp. SC-10 in a biphasic reaction system was investigated in this study. Model oil containing dibenzothiophene was used as the sole sulfur source of bacterium. The results showed that 81.43% of the sulfur in model oil with initial sulfur content of 174 mg/l could be removed within 5 days. Gordonia sp. SC-10 had high desulfurization rate for model oils with low sulfur contents, which would reduce sulfur concentration to below 5 mg/l. Biodesulfurization process might be a potential method to obtain fuels with ultra-low sulfur contents.
研究领域:
环境生物学
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微生物学
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产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2016
·
IF 4.165
Gu Haiping,Lou Jun,Wang Haizhen,Yang Yu,Wu Laosheng,Wu Jianjun,Xu Jianming
Reducing phenanthrene (PHE) in the environment is critical to ecosystem and human health. Biodegradation, biosorption, and the trans-membrane transport mechanism of PHE by a novel strain, Massilia sp. WF1, and an extensively researched model fungus, Phanerochaete chrysosporium were investigated in aqueous solutions. Results showed that the PHE residual concentration decreased with incubation time and the data fitted well to a first-order kinetic equation, and the t1/2 of PHE degradation by WF1, spores, and mycelial pellets of P. chrysosporium were about 2 h, 87 days, and 87 days, respectively. The biosorbed PHE was higher in P. Chrysosporium than that in WF1, and it increased after microorganisms were inactivated and inhibited, especially in mycelial pellets. The detected intracellular auto-fluorescence of PHE by two-photon excitation microscopy also proved that PHE indeed entered into the cells. Based on regression, the intracellular (Kdin) and extracellular (Kdout) dissipation rate constants of PHE by WF1 were higher than those by spores and mycelial pellets. In addition, the transport rate constant of PHE from outside solution into cells (KinS/Vout) for WF1 were higher than the efflux rate constant of PHE from cells to outside solution (KoutS/Vin), while the opposite phenomena were observed for spores and mycelial pellets. The amount of PHE that transported from outside solution into cells was attributed to the rapid degradation and active PHE efflux in the cells of WF1 and P. Chrysosporium, respectively. Besides, the results under the inhibition treatments of 4°C, and the presence of sodium azide, colchicine, and cytochalasin B demonstrated that a passive trans-membrane transport mechanism was involved in PHE entering into the cells of WF1 and P. Chrysosporium.
研究领域:
环境生物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Catalysts
·
2022
·Vol. 12
·期 12
·1653
·
IF 4.501
Jiameng Zhang,Zhiliang Yu,Yaling Gao,Meini Wang,Kai Wang,Tao Pan
A newly isolated cadmium (Cd)-resistant bacterial strain from herbicides-polluted soil in China could use atrazine as the sole carbon, nitrogen, and energy source for growth in a mineral salt medium (MSM). Based on 16S rRNA gene sequence analysis and physiochemical tests, the bacterium was identified asArthrobactersp. and named ST11. The biodegradation of atrazine by ST11 was investigated in experiments, with the compound present either as crystals or dissolved in di(2-ethylhexyl) phthalate (DEHP) as a non-aqueous phase liquid (NAPL). After 48 h, ST11 consumed 68% of the crystalline atrazine in MSM. After being dissolved in DEHP, the degradation ratio of atrazine was reduced to 55% under the same conditions. Obviously, the NAPL-dissolved atrazine has lower bioavailability than the crystalline atrazine. Cd2+at concentrations of 0.05–1.5 mmol/L either had no effect (<0.3 mmol/L), slight effects (0.5–1.0 mmol/L), or significantly (1.5 mmol/L) inhibited the growth of ST11 in Luria-Bertani medium. Correspondingly, in the whole concentration range (0.05–1.5 mmol/L), Cd2+promoted ST11 to degrade atrazine, whether crystalline or dissolved in DEHP. Refusal to adsorb Cd2+may be the main mechanism of high Cd resistance in ST11 cells. These results may provide valuable insights for the microbial treatment of arable soil co-polluted by atrazine and Cd.Keywords:atrazine;Arthrobactersp. ST11;biodegradation;nonaqueous-phase liquid;cadmium
研究领域:
环境科学
·
微生物学
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产品关联
DOI
高影响因子
Polymers
·
2023
·Vol. 15
·期 13
·2835
·
IF 5
Yijuan Chang,Zhihui Wu
This study aimed to investigate the impact of different sandpaper sanding meshes on the mechanical and optical properties of microencapsulatedTiliasp. film. An orthogonal experiment revealed that sanding between primers had the most significant effect. Furthermore, an independent experiment implied that increasing the mesh size resulted in decreased surface roughness and decreased color difference, elongation at break, and gloss after liquid resistance. In the aging test, the color difference of the paint film increased with the aging time, and the gloss tended to stabilize. Additionally, the anti-aging gloss of 240 mesh sandpaper used between primers remained relatively stable. The paint film sanded with 240 mesh sandpaper between primers displayed small and regular cracks after temperature and UV aging. Overall, the paint film demonstrated good comprehensive performance when sanded with 240 mesh between primers, 240 mesh between primer/topcoat, and 1000 mesh for topcoat. Self-repairing microcapsules showed better repair efficacy on the coating. This study provides a technical reference for the development of self-repairing coatings.
研究领域:
材料科学
·
涂层技术
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-10
·
IF 5.228
Wu Haobo,Shen Jinyou,Wu Ruiqin,Sun Xiuyun,Li Jiansheng,Han Weiqing,Wang Lianjun
The highly recalcitrant 1H-1,2,4-triazole (TZ) is widely used in the synthesis of agricultural pesticide and considered to be an environmental pollutant. In this study, a novel strain NJUST26 capable of utilizing TZ as the sole carbon and nitrogen source, was isolated from TZ-contaminated soil, and identified as Shinella sp. The biodegradation assays suggested that optimal temperature and pH for TZ degradation by NJUST26 were 30 °C and 6–7, respectively. With the increase of initial TZ concentration from 100 to 320 mg L −1 , the maximum volumetric degradation rate increased from 29.06 to 82.96 mg L −1 d −1 , indicating high tolerance of NJUST26 towards TZ. TZ biodegradation could be accelerated through the addition of glucose, sucrose and yeast extract at relatively low dosage. The main metabolites, including 1,2-dihydro-3H-1,2,4-triazol-3-one (DHTO), semicarbazide and urea were identified. Based on these results, biodegradation pathway of TZ by NJUST26 was proposed, i.e., TZ was firstly oxidized to DHTO, and then the cleavage of DHTO ring occurred to generate N-hydrazonomethyl-formamide, which could be further degraded to biodegradable semicarbazide and urea.
研究领域:
环境微生物学
·
生物修复
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有机化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 9
·1-10
·
IF 5.7
Hong Tingting,Liu Xing,Ji Yibing,Tan Songwen,Cai Zhiqiang
Novel chiral capillary electrochromatography (CEC) microsystems were constructed based on Aspergillus sp. CM96. As a newly discovered intrinsic characteristic of the cell, cell chirality occupies an essential position in life evolution. Aspergillus sp. CM96 spore (CM96s) was chosen as a proof of concept to develop chiral capillary columns. Interestingly, various types of amino acid (AA) enantiomers were baseline separated under the optimized conditions. Furthermore, the time-dependent chiral interactions between AAs and CM96s were explored in a wider space. Pectinases generated from Aspergillus sp. CM96 fermentation were immobilized onto graphene oxide–functionalized capillary silica monoliths for separating AA enantiomers. Molecular docking simulations were performed to explore chiral separation mechanisms of pectinase for AA enantiomers. These results indicated that Aspergillus sp. CM96-based CEC microsystems have a significant advantage for chiral separation. Graphical abstract
研究领域:
分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）