Acta Biomaterialia
·
2024
·
IF 9.4
Liang Yu,Mingzu Zhang,Jinlin He,Xingwei Sun,Peihong Ni
ABSTRACT Anticancer drugs used for systemic chemotherapy often exhibit off-target toxicity and uncontrolled drug release due to their lack of targeting. To improve the bioavailability of drugs and reduce side effects, we have developed a mixed micelle of nanomedicine composed of two prodrugs with surface modified monoclonal antibody for cancer therapy. In this system, Nimotuzumab was used as targeting ligands of the mixed micelles (named as DCMMs) that is composed of polymer-doxorubicin prodrug (abbreviated as PEG- b -P(GMA- ss -DOX)) and maleimide polyethylene glycol-chlorin e6 (abbreviated as Mal-PEG-Ce6). The mixed micelles modified with Nimotuzumab (named as NTZ-DCMMs) bind to overexpressed EGFR receptors on Hepatoma-22 (H22) cells. Disulfide bonds in PEG- b -P(GMA- ss -DOX) are disrupted in tumor microenvironment, inducing the reduction-responsive release of DOX and leading to tumor cell apoptosis. Simultaneously, Chlorin e6 (Ce6) produced plenty of singlet oxygen ( 1 O 2 ) under laser irradiation to kill tumor cells. In vivo biological distribution and antineoplastic effect experiments demonstrate that NTZ-DCMMs enhanced drug enrichment at tumor sites through targeting function of antibody, dramatically suppressing tumor growth and mitigating cardiotoxicity of drugs. All results prove that NTZ-DCMMs have the ability to actively target H22 cells and quickly respond to tumor microenvironment, which is expected to become an intelligent and multifunctional drug delivery carrier for efficient chemotherapy and photodynamic therapy of hepatoma. Statement of significance Anticancer drugs used for systemic chemotherapy often exhibit off-target toxicity due to their lack of targeting. Therefore, it's necessary to develop effective, targeted, and collaborative treatment strategies. We construct a mixed micelle of nanomedicine based on two polymer prodrugs and modified with monoclonal antibody on surface for cancer therapy. Under the tumor cell microenvironment, the disulfide bonds of polymer- ss -DOX were broken, effectively triggering DOX release. The photosensitizer Ce6 could generate a large amount of ROS under light, which synergistically promotes tumor cell apoptosis. By coupling antibodies to the hydrophilic segments of polymer micelles, drugs can be specifically delivered. Compared with monotherapy, the combination of chemotherapy and photodynamic therapy can significantly enhance the therapeutic effect of liver cancer.
研究领域:
药学
·
纳米技术
·
癌症研究
·
生物医学工程
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DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-11
·
IF 4.259
Zhao Hongting,Li Huihui,Hao Shuangying,Chen Jiping,Wu Jing,Song Chuanhui,Zhang Meng,Qiao Tong,Li Kuanyu
Friedreich ataxia is a progressive neurodegenerative disease caused by the expansion of GAA trinucleotide repeats within the first intron of the FXN gene, which encodes frataxin. The pathophysiology of the disease is thought to be derived from the decrease of Fe-S cluster biogenesis due to frataxin deficiency. There is currently no effective treatment for the disease. In our study, we demonstrated that treatment with the mitochondrion-targeted peptide SS-31 reduced frataxin deficiency-induced oxidative stress in lymphoblasts and fibroblasts derived from patients. Interestingly, SS-31 treatment translationally upregulated the protein level of frataxin in a dose-dependent manner. Furthermore, SS-31 treatment increased the enzymatic activities of the iron-sulphur enzymes, including aconitase and complex II and III of the respiratory chain. Further evaluation of the quality of mitochondria showed that mitochondrial membrane potential, ATP content, NAD + /NADH, and the morphology of mitochondria all improved. Our results suggest that SS-31 might potentially be a new drug for the early treatment of Friedreich ataxia.
研究领域:
神经退行性疾病
·
分子生物学
·
线粒体生物学
·
遗传病
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DOI
PubMed
高影响因子
Polymer Chemistry
·
2012
·Vol. 4
·期 1
·156-165
·
IF 5.321
Lejiao Jia,Zhenyu Li,Dianrui Zhang,Qiang Zhang,Jingyi Shen,Hejian Guo,Xiaona Tian,Guangpu Liu,Dandan Zheng,Lisi Qi
The chitosan oligosaccharide-based disulfide-containing polyethylenimine derivative PEG-ss-COS-ss-PEI was synthesized and evaluated as a nonviral gene delivery carrier. The structure of the obtained polymers was confirmed by 1H NMR and FTIR. PEG-ss-COS-ss-PEI copolymers could effectively condense DNA into small particles with average diameters less than 120 nm and the zeta potential of +15.7 mV at the N/P ratio of 15/1. Additionally, the resultant polyplexes showed excellent colloidal stability against 150 mM NaCl and had a better buffering capacity of ∼44%, which was more than double the buffering capacity of PEI1.8k (∼20%). In the presence of 10 mM glutathione (GSH), however, polyplexes of PEG-ss-COS-ss-PEI were rapidly unpacked, as revealed by significant increase of particle sizes to over 800 nm. In vitro experiments revealed that the PEG-ss-COS-ss-PEI copolymers not only had much lower cytotoxicity, but also displayed high transfection efficiency as compared to the control branch 25 kDa PEI. This study indicates that a reducibly degradable copolymer PEG-ss-COS-ss-PEI composed of low molecular weight PEI, chitosan oligosaccharide and PEG via disulfide-containing linkages can be a promising gene delivery carrier.
研究领域:
生物材料学
·
基因治疗
·
药剂学
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DOI
高影响因子
TEXTILE RESEARCH JOURNAL
·
2021
·
IF 1.82
Hongbin Li,Wenying Shi,Tengfei Li,Qiyun Du,Haixia Zhang,Xiaohong Qin
With excellent mechanical properties, large porosity, and permeability, stainless steel (SS) fiber nonwoven felt has outstanding application advantages in high-temperature filtration and purification. However, the pore size of the SS nonwoven felt, which is directly determined by the diameter of the produced fiber stacked inside, usually varies from tens of microns to several microns. Low filtration accuracy greatly limits its application in the fields of fine separation and purification. In this study, the separation performance of SS fiber nonwoven felt was improved by the coating of a non-isotropic porous SS membrane layer via the immersion precipitation phase inversion-sintering method. The effects of sintering temperature on pore structure, surface wettability, separation performance, and mechanical properties of the coated SS nonwoven felt were characterized by scanning electron microscope (SEM), water contact angle (WCA), water permeability, and tensile test, respectively. The results suggest that with the increase of sintering temperature from 1000°C to 1200°C, both porosity and pore size reduce gradually. The WCA value shows an increase from 31.4 to 62.3° and pure water flux shows a corresponding decrease from 2562 to 889 L . m−2 . h−1. The sintering temperature has a negative effect on the mechanical strength of the coated SS fiber nonwoven felt, which is mainly determined by the mechanical properties of the sintered SS fiber nonwoven felt substrate. The coated SS fiber nonwoven felt exhibits a long-term durable separation performance even after frequent combined physical washing and chemical cleaning when applied in the treatment of potato starch wastewater.
研究领域:
材料科学
·
过滤技术
·
环境工程
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产品关联
DOI
高影响因子
JOURNAL OF THE TEXTILE INSTITUTE
·
2023
·
IF 1.7
Xianhua Zhang,Fengting Yao,Xuling Jin,Bing Yu,Jinlong Zhou
Using deionized water as a solvent, the polyvinyl alcohol/silk sericin (PVA/SS) mixed solution was prepared, and the PVA/SS composite nanofiber membrane was spun by the electrostatic spinning method. The characteristics and properties of the PVA/SS composite nanofiber membrane untreated and treated with methanol and ethanol steam were analyzed by scanning electron microscope, infrared spectrum and X-ray diffraction. The results showed that the morphology of the PVA/SS composite nanofibers was fine in this experiment. The average diameter and standard deviation of diameter first increased, then decreased and at last increased with the increase of the SS content. The average diameter of nanofibers treated with methanol and ethanol steam was smaller than that without treatment. The infrared spectrum showed that the peak value of SS in the PVA/SS composite nanofibers treated with methanol steam and ethanol steam for 3 d changed. X-ray diffraction showed that the crystallization of the PVA/SS composite nanofiber membrane after being treated with methanol and ethanol vapor was changed. The results provide theoretical support for biomedical materials and tissue engineering scaffolds.
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DOI
高影响因子
AICHE JOURNAL
·
2023
·Vol. 69
·期 8
·e18105
·
IF 3.7
Mide Luo,Zong Lu,Yali Zhao,Yufei Wang,Yanying Wei,Haihui Wang
Accurately constructing membranes based on two-dimensional (2D) materials on commercial porous substrates remains a significant challenge for H 2 purification. In this work, a series of tubular 2D MXene membranes are prepared on commercial porous stainless steel substrates via fast electrophoretic deposition. Compared with other methods, such as filtration or drop coating, and so on. such preparation route shows the advantages of simple operation, high efficiency for membrane assembly (within 5 min) with attractive reproducibility, and ease for scale-up. The tubular MXene membranes present excellent gas separation performance with hydrogen permeance of 1290 GPU and H 2 /CO 2 selectivity of 55. Furthermore, the membrane displays extremely stable performance during the long-term test for more than 1250 h, and about 93% of the membranes from one batch have exceeded the DOE target for CO 2 capture. Most importantly, this work provides valuable referential significance for other 2D materials-based membranes for future application development.
研究领域:
材料科学
·
能源工程
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产品关联
DOI
高影响因子
Results in Engineering
·
2024
·
IF 6
Thi Thi Mar,Yunlong Xue,Yu Chang,Zijian Yu,Zhongxiao Du,Bing Cao,Rui Zhang
Pervaporation emerges as a promising technique for brine treatment. In this investigation, we employed a PVDF substrate layer and applied various coating solutions using a simple spray technique to produce a composite pervaporation membrane. The composite membrane's chemical structure, morphology, hydrophilicity, swelling property, surface roughness, and crosslinking conditions were thoroughly investigated. Notably, the PVDF/PEI/P(SS-MA) composite membrane exhibited outstanding desalination performance, demonstrating high salt rejection and flux of 308.7 ± 13.8 kg m −2 h −1 when subjected to a 3.5 wt% sodium chloride solution. Even at an elevated brine concentration of 20 wt%, the membrane maintained a commendable flux of 88.76 ± 5.4 kg m −2 h −1 at 72 °C. These findings underscore the efficacy of the developed composite membrane for brine desalination applications.
研究领域:
材料科学
·
环境工程
·
化学工程
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产品关联
DOI
高影响因子
HIGH PERFORMANCE POLYMERS
·
2017
·
IF 1.179
Shan Huang,Xiao Hou,Jiaojiao Li,Xiujuan Tian,Qing Yu,Zhongwei Wang
A phosphorous/nitrogen-containing diphenylphosphine oxide (DPO) derivative (DPO-SS) was designed and synthesized via a two-step reaction of 4,4′-diaminodiphenylsulfone, 2-hydroxy-benzaldehyde, and DPO. The structure of DPO-SS was confirmed by Fourier transform infrared spectroscopy (FTIR), 1H and 31P nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry (MS). DPO-SS was used as a flame retardant and curing agent for copolymerizing with diglycidyl ether of bisphenol-A. Thermal and flame-retardant properties of the obtained flame-retardant epoxy resin (F-EP) were investigated by thermogravimetric analysis, dynamic thermomechanical analysis, limited oxygen index (LOI) measurement, vertical burning test (UL-94), and cone calorimeter test. Results indicated that all F-EP samples exhibited excellent thermal stability and flame-retardant property. Especially for F-EP with P content of 0.7 wt% (denoted as EP/P-0.7), it achieved high LOI values (32.4%) and UL-94 V-0 rating. Compared with pure EP, all F-EP samples showed lower heat release rate, total heat release, total smoke produce, and little Tg fluctuation. In order to study the flame-retardant mechanism, the char residues were investigated by FTIR, scanning electron microscopy, and energy-dispersive spectrometer analysis. The results manifested that DPO-SS acted as flame retardant in both gas phase and condensed phase. Water absorption properties of pure EP and F-EP were also compared through immersion experiments. Results showed that EP/P-0.7 sample had apparently lower water absorptivity than pure EP.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2016
·
IF 3.485
Changyu He,Qinglai Yang,Lianjiang Tan,Bingya Liu,Zhenggang Zhu,Bing Gong,Yu-Mei Shen,Zhifeng Shao
The need for smart materials in the area of biotechnology has accelerated the development of stimuli-responsive copolymer micelles. Here, we reported a novel dual-stimuli-responsive block copolymer PEG-DMTK-SS-PLA with both dimethyl thioketal (DMTK) and disulfide linkage incorporated into the backbone, capable of triggering fast drug release properties upon both oxidative (H 2 O 2 ) and reductive (GSH) environment. The CMC values of these copolymer micelles ranging from 0.051 to 0.087 mg mL −1 , the average diameters are from 34 nm to 55 nm and 65 nm to 198 nm for blank and DOX-loaded micelles respectively. MTT assay conducted in NIH 3T3 cells showed the low cytotoxicity of these micelles even when the concentration reached up to 500 μg/mL. Considering tumor microenvironment’s diverse in kinds of tumor cells, fluorescence microscopy, flow cytometry and MTT activity analysis were conducted in several cancer cells (e.g., cervix, lung, gastric, and colon cancer cells), and further confirmed that the dual responsive PEG-DMTK-SS-PLA micelles were degraded much faster than that of non-responsive PEG-PLA and single responsive PEG-SS-PLA and PEG-DMTK-PLA micelles. These results indicate that the dual-responsive micelles are promising for efficient anticancer drug delivery.
研究领域:
生物技术
·
药剂学
·
肿瘤学
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DOI
高影响因子
NANOTECHNOLOGY
·
2013
·Vol. 24
·期 34
·345502
·
IF 3.842
Guiying Liu,Yong Shao,Jian Peng,Wei Dai,Lingling Liu,Shujuan Xu,Fei Wu,Xiaohua Wu
Double-stranded DNAs (ds-DNAs) have been identified as efficient templates favoring the formation of fluorescent copper nanoparticles (Cu NPs). Herein, we have tried to synthesize fluorescent Cu NPs using single-stranded DNAs (ss-DNAs) as templates and to identify the critical DNA sequences. By comparing the results using homopolymer DNAs, hairpin DNAs, and pristine ss-DNAs as templates, we found that DNA thymine base plays a dominant role in producing red-emissive fluorescent Cu NPs on ss-DNA templates. The thymine-dependent growth of the fluorescent Cu NPs is confirmed by Hg2+ mediated T–T base pair in comparison with the other non-specific metal ions, which could be developed into a practical sensor for turn-on fluorescence detection of Hg2+ with a high selectivity. The mechanism is briefly discussed according the DNA sequence-dependent formation of fluorescent Cu NPs. This work demonstrates the sequence role in producing fluorescent Cu NPs that could serve as promising fluorescent nanoprobes in biosensing and DNA-hosted Cu nanomaterials.
研究领域:
纳米技术
·
生物化学
·
分析化学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Jiaojiao Chen,Yarong Song,Wenqian Yang,Jianhui Guo,Saihui Zhang,Dong Wan,Yonghui Liu,Jie Pan
Chemotherapy remains the primary treatment for cancer; however, conventional chemotherapeutic drugs have several shortcomings, including poor solubility, lack of targeting, low bioavailability, and high toxicity to healthy tissues. In order to overcome these limitations, drug delivery systems (DDS) have emerged as a promising alternative. In this study, we synthesized TPGS3350-PVGLIG-SS-DOX polymeric micelles that incorporate both extracellular MMP2/9 enzyme response and intracellular reduction response at a nanoscale level. The micelles remained stable throughout the blood circulation and only aggregated at the tumor sites, thanks to the enhanced permeability and retention (EPR) effect. Moreover, the tumor tissue showed a high expression of MMP2/9 enzymes, which resulted in the cleavage of the PVGLIG peptide sequence, ultimately leading to an improved phagocytosis efficiency. Under the action of high intracellular GSH concentration, the disulfide bond was broken and DOX was released to induce tumor cell death. The TPSD&D micelles exhibited good tumor inhibitory activity in vivo and significantly reduced toxic side effects on normal tissues. Our work provides a simple method to assemble tumor-targeted prodrug micelles to improve cancer therapy, which holds potential for clinical applications in antitumor therapy.
研究领域:
药剂学
·
肿瘤学
·
生物材料学
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产品关联
DOI
高影响因子
Soft Science
·
2026
·Vol. 6
·期 3
·N
·
IF 15.9
Binglu Zhong,JieLu1,Xingyu Jiang,Jie Wu1,Dianjue Liu1,Shaobo Ji1,Zi Wang,Rui Zhang1,Lizhen Huang,Lifeng Chi
Organic electrochemical transistors (OECTs) have emerged as promising candidates for bioelectronics because of their efficient ionic–electronic coupling. However, the realization of balanced ionic–electronic transport with satisfactory stability is challenging. Herein, we report a dual-solvent-assisted blade-coating strategy to modulate the morphology of hydrophilic ethylene glycol-grafted polythiophene films. The effects of the film thickness and crystallinity on OECT performance are systematically investigated. The well-ordered and uniform edge-on morphology achieved with the dual-solvent system facilitates efficient charge transport without impeding ion penetration, leading to efficient mixed ionic–electronic transport. More importantly, the operational stability is substantially enhanced compared with films processed using a single solvent, such that even thin films with thicknesses below 20 nm maintain more than 90% of their on-current after thousands of operational cycles. The optimized OECTs are successfully demonstrated for electrocardiogram (ECG) monitoring. This study provides an effective morphology engineering strategy for high-performance OECTs in wearable bioelectronics.
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产品关联
DOI
高影响因子
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