SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Jie Cheng,Xiao-Ou Su,Caiqin Han,Shi Wang,Peilong Wang,Su Zhang,Jianchun Xie
Salbutamol (SAL) is a β 2 -adrenergic receptor, which could produce leaner meat with a higher muscle to fat ratio for livestock, has been prohibited for use in animal farming in many countries because of its harmful effect to the health of consumers. In this study, we present a novel method based on surface-enhanced Raman spectroscopy (SERS) for the sensitive and quantitative detection of trace amount of SAL in animal urine samples by reoxidized graphene oxide/gold nanoparticle hybrids (rGO/AuNPs) substrates and the immunomagnetic bead sample treatment. Compared with the regular graphene/gold nanoparticles (GO/AuNPs) substrates, the rGO/AuNPs substrates by the second oxidization pretreatment exhibited an excellent SERS activity with an enhancement factor of ∼10 8 . Under the optimal detection conditions, the limit of detection of SAL in urine sample is 0.5 ng mL −1 with a linear response of the matrix-matched calibration standard curves range from 1.0–20 ng mL −1 , and the recovery of SAL from spiked urine samples are in the range of 85.2–92.5% with the relative standard deviations (RSD) values <3.18%. The inter- and intra- day detection precisions is less than 6.28%, and the storage time of the substrates is no less than 4 weeks under 4 °C temperature. The results of the proposed SERS detection method agree well with those determined by LC–MS/MS, while the detection time is significantly reduced to 15 min/sample. All these are demonstrated to be a reliable, rapid, simple and ultrasensitive analytical method for detecting SAL in urine samples, and could also be applied for the routine monitoring of the illicit use of SAL in animal farming.
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高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Shuang Zhao,Tong Bu,Kairong Yang,Zhihao Xu,Feier Bai,Kunyi He,Lihua Li,Li Wang
Salbutamol (SAL), a β-2 adrenoreceptor agonist, is an unpopular addition to livestock and poultry, causing several side effects to human health. Thus, it is very important to develop a simple and rapid analytical method to screen SAL in the field of food safety. Here, we present an immunochromatographic assay (ICA) method for sensitively detecting SAL with polydopamine-decorated iridium oxide nanoparticles (IrO2@PDA NPs) as a signal tag. The IrO2@PDA with excellent hydrophilicity, biocompatibility, and stability was synthesized by oxidating self-polymerization of dopamine hydrochloride (DAH) on the surface of IrO2 NPs and used to label monoclonal antibodies (mAbs) through simple physical adsorption. Compared with IrO2 NPs, the IrO2@PDA also possessed superior optical properties and higher affinity with mAbs. With the proposed method, the limit of detection for SAL was 0.002 ng/mL, which was improved at least 24-fold and 180-fold compared with the IrO2 NPs-based ICA and conventional gold nanoparticles-based ICA, respectively. Furthermore, the SAL residuals in pork, pork liver, and beef were successfully detected by the developed biosensor and the recoveries ranged from 85.56% to 115.56%. Briefly, this work indicated that the powerful IrO2@PDA-based ICA can significantly improve detection sensitivity and has huge potential for accurate and sensitive detection of harmful small molecules analytes in food safety fields.
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 3
·961-968
·
IF 4.58
Dong Tiantian,Tang Qinghui,Zhao Kang,Deng Anping,Li Jianguo
The authors describe a dual signal amplification electrochemiluminescent (ECL) immunoassay for ultrasensitive determination of the β-adrenergic agonist salbutamol (SAL). It is based on the use of gold nanoparticles (AuNPs) and silica-coated quantum dots of the type CdSe@SiO 2 . The CdSe@SiO 2 nanoparticles (NPs) were synthesized by water-in-oil reversed-phase microemulsion. Amino-modified NPs were then prepared and used as an ECL signal probe for detection of SAL in the presence of K 2 S 2 O 8 . The gold NPs with their large specific surface carry the antigen, facilitate electron transfer and improve the electrochemical reaction efficiency of QDs and K 2 S 2 O 8 . The ECL immunosensor was fabricated by coating the AuNPs with antigen, and then SAL competed with SAL antigen for the binding sites of the antibody on the CdSe@SiO 2 NPs. Under optimized conditions, the ECL intensity is linearly related to the logarithm of the SAL concentration in the range from 0.001 to 1000 ng mL −1 , with a lower detection limit of 0.17 pg mL −1 (at an S/N ratio of 3). The assay has good operational stability, is highly sensitive and selective. It is judged to possess a wider scope in that it may be applied to many other small molecule analytes for which adequate antibodies are available.
Schematic of a dual signal amplification electrochemiluminescent immunoassay for ultrasensitive detection of the β-adrenergic agonist salbutamol based on the use of gold nanoparticles and CdSe@SiO 2 .
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BIOMEDICAL CHROMATOGRAPHY
·
2015
·Vol. 29
·期 12
·1834-1841
·
IF 1.723
Hua Fan,Chaozhan Wang,Yinmao Wei
Salbutamol (SAL) is the most widely used β 2 -agonist drug for asthma and chronic obstructive pulmonary patients, but it is also often abused as feed additive. In recent years, the abuse of SAL has led to a large number of food safety incidents. Therefore, the monitoring of SAL residues in animal products is very important. A highly selective boronate affinity magnetic adsorbent was synthesized and developed for detection of trace levels of SAL residues in pig tissue samples. The obtained Fe 3 O 4 @SiO 2 @FPBA(4-formylphenylboronic acid) magnetic adsorbent showed good adsorption ability to catechol and SAL, and then it was successfully applied as special magnetic solid-phase phase extraction adsorbent coupled with high-performance liquid chromatography (HPLC) for simultaneous isolation and determination of cis -diol compounds. The binding capacity of catechol and SAL reached 96 and 50 µmol/g, respectively. The method was successfully established for the detection of trace levels of SAL in pig tissue samples. The linear range extended from 0.32 to 800 µg/kg ( R 2 = 0.9994). The limit of detection of SAL was 0.19 µg/kg. The recoveries were satisfactory (89.5–108.0%) at three spiked levels with RSD between 2.1 and 11.3%. These results indicated that the method has potential for enrichment and detection of trace levels of SAL residual in animal food products. Copyright © 2015 John Wiley & Sons, Ltd.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ying Zhou,Donghui Wang,Dingnan Wang,Yang Wang,Yang Li,Jie Li,Yiming Zhang
Mimetic recognition materials for rapid and selective analysis of foodborne contamination from complex foodstuff becomes a hot research topic in the field of food safety. In this work, borate-affinity-functionalized MOF/molecularly imprinted polymer paper-based microfluidic chips (FSU-BA@MIP) were prepared by growing functionalized MOFs on paper surface with 3-carboxyphenylboronic acid (3-CPBA) as the ligands, followed by coating of a MIP layer of salbutamol (SAL) through a borate-affinity surface imprinting strategy. Using the obtained paper-based chip as a mimetic recognition and specific enrichment module, the fluorescence sensing platform achieved visual rapid quantitative analysis of SAL with a linear range of 0.1 × 10 −2 –5.0 mg L −1 . The limits of detection and quantification were calculated to be 0.095 and 0.290 μg kg −1 , respectively, and the sample loading via lateral flow aid could be completed within 30 min. The obtained MOFs/MIPs paper chips provide an effective way for the rapid analysis of harmful contaminants from different animal-derived foods.
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高影响因子
FOOD CONTROL
·
2016
·
IF 3.388
Ming Li,Yuanyuan Zhang,Yonglai Xue,Xia Hong,Yin Cui,Zhenjiang Liu,Daolin Du
Lanthanide ions, the excellent fluorescence markers, have the potential to simplify the performance of multi-analytes analysis. In this work, a powerful time-resolved fluoroimmunoassay (TRFIA) based on dual-labeled format were performed for detecting clenbuterol and salbutamol simultaneously in water and swine feed samples. Europium (Eu 3+ )-labeled goat anti-mouse IgG and Samarium (Sm 3+ )-labeled goat anti-rabbit IgG were prepared and used as fluorescent probes. Under optimal conditions, the half-maximal inhibition concentration (IC 50 ) and the limit of detection (LOD, IC 10 ) of the TRFIA were 0.263 and 0.007 ng/mL for clenbuterol, and 0.179 and 0.014 ng/mL for salbutamol, respectively. The cross-reactivities of the TRFIA between these two β 2 -agonists and with their analogues were negligible, thus indicated high specificity and promoted the simultaneous determination. The spiked recoveries were 72.2%–102.2% with relative standard deviations (RSDs) of 3.4–13.8% for clenbuterol and salbutamol in water and swine feed samples. Furthermore, the results of TRFIA correlated well with those obtained by HPLC. The proposed dual-labeled TRFIA was a satisfactory tool for simultaneous, sensitive, efficient and labour-saving detection of clenbuterol and salbutamol in water and swine feed samples.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Dan Lin,Anqi Liu,Lei Pan,Fan Yang,Liang Yang,Changlong Jiang
Sensors based on multiple fluorescence materials have been widely applied in detection because of reliability and reproducibility. High-performance on-site sensing strategies are vital to public safety and human health. Here, by modifying the carbon dots (CDs) with melamine (MA) molecules, we developed a novel dual-recognition fluorescence sensor for efficient and sensitive detection of salbutamol. Upon increasing salbutamol concentration, the fluorescence intensity of blue emission under 365 nm excitation decreased, while the yellow emission under 460 nm kept enhancing. The quench of blue emission leads to the yellow fluorescence enhancement by Fluorescence resonance energy transfer (FRET) from monodisperse CDs to clustered CDs. Both the two emission-intensity showed linear relationship with salbutamol concentration within a certain concentration range. Dual detection of fluorescence signals under 365 nm and 460 nm, with detection limits (3δ/K) as low as 7.12 and 16.67 nM, is possible. Machine learning is then employed to analyze its detection performance. Therefore, the quantitative detection of salbutamol can be achieved. Moreover, the dual-recognition assay endows salbutamol detection built-in self-calibration to ensure high assay sensitivity and precision. We demonstrated the application in the fluorescence tracking of salbutamol in real samples with satisfactory results. The study would anticipate this dual-recognition fluorescence sensing system would inspire fast high-precision sensor design for environmental, safety and health monitoring.
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Pharmaceutics
·
2020
·Vol. 12
·期 1
·78
·
IF 4.421
Rui Zhang,Junhua Hu,Liangjun Deng,Sha Li,Xi Chen,Fei Liu,Shanping Wang,Khaja Shameem Mohammed Abdul,Huimin Beng,Wen Tan
Inhalation therapy with a nebulizer is widely used in chronic respiratory disease. Mixing inhalation solutions/suspensions for simultaneous inhalation is more convenient and might simplify the administration procedure. However, there are no data available to address the in vitro aerosol characteristics and physico-chemical compatibility of Combivent®(containing Salbutamol and Ipratropium bromide) with other inhalation solutions/suspensions. In order to investigate the in vitro aerosol characteristics and physico-chemical compatibility of Combivent®with Budesonide, Beclomethasone, andN-acetylcysteine, the appearance, pH, osmotic pressure, chemical stability, mass median aerodynamic diameter (MMAD), fine particles fraction (FPF), particle size corresponding to X50 (particle size, which accounts for 50% of the total cumulative percentage of volume of all particles), delivery rate, and total delivery of the mixed inhalation solution/suspension were tested. There was no change in the appearance such as a change in color or precipitation formation at room temperature. The pH, osmolality, and chemicals of the mixtures were stable for 24 h after mixing. There were no significant differences between Combivent®, Budesonide, Beclomethasone,N-acetylcysteine, and the mixtures in MMAD, FPF, X50, the delivery rate, and the total delivery. This indicates that the mixtures were physically and chemically compatible. The mixing did not influence the particle size, distribution, or delivery compatibility of the mixtures.Keywords:inhalation;nebulizer;physico-chemical;particle size and distribution;delivery compatibility;Combivent®;Budesonide;Beclomethasone;N-acetylcysteine
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PubMed
高影响因子
Food Analytical Methods
·
2016
·Vol. 9
·期 12
·3396-3406
·
IF 2.167
Zhang Xiang,Chu Yanxin,Yang Hong,Zhao Kang,Li Jianguo,Du Haijing,She Pei,Deng Anping
Salbutamol (SAL), a kind of β-agonist which can enhance the lean meat-to-fat ratio, has been inhibited as an additive used in animal feeds for livestock production in many countries due to its harmful effect to the consumers. In this study, an ultrasensitive and specific competitive immunochromatographic test (ICT) integrated with surface-enhanced Raman scattering (SERS) for the detection of SAL was described. The immunoprobe was prepared by immobilizing polyclonal antibody against SAL on the surface of Au@Ag nanoparticles in which the Raman reporter (4-mercaptobenzoic acid, MBA) had been sandwiched. After ICT procedures, the specific SERS signals generated from MBA on the test line of the ICT strip were measured for the quantitative determination of SAL. The assay was completed in 15 min. The IC 50 and the limit of detection (LOD) values of the assay for SAL were 0.028 ng mL −1 and 3.0 pg mL −1 , respectively. There was no cross-reactivity (CR) of the assay with other three β-agonists (clenbuterol, phenylethanolamine A, and ractopamine), showing high specificity of the assay. Spiking experiments indicated that the average recoveries ( n = 3) of SAL from swine feed, meat, and urine samples were in ranges of 98.4–105.2 % with the relative standard deviations (RSDs) of 1.7–7.8 %. The results demonstrated that the proposed ICT was a feasible method for ultrasensitive and specific detection SAL in swine feed, meat and urine samples, and could be extended for the detection of other target analytes.
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危险品化学品经营许可证（带存储）