JOURNAL OF FOOD PROTECTION
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2014
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IF 1.797
Hongmei Zhang,Wenyuan Zhou,Wenyan Zhang,Anlin Yang,Yanlan Liu,Yan Jiang,Shaosong Huang,Jianyu Su
Biofilms are significant hazards in the food industry. In this study, we investigated the effects of food additive such as citral, cinnamaldehyde, and tea polyphenols on mixed biofilm formation by foodborne Staphylococcus aureus and Salmonella serotype Enteritidis. The adhesion rates of mixed strains in sub-MIC of additives were determined by a microtiter plate assay and bacterial communication signal autoinducer 2 (AI-2) production via a bioluminescence reporter Vibrio harveyi BB170. The structure of mixed biofilm was analyzed using scanning electron microscopy. The effect of the disinfectants hydrogen peroxide, sodium hypochlorite, and peracetic acid was tested on the mixed biofilm. Our results demonstrated that citral, cinnamaldehyde, and tea polyphenols were able to significantly inhibit mixed biofilm formation, while citral could reduce the synthesis of AI-2. Conversely, we observed a significant increase in AI-2 mediated by cinnamaldehyde. Tea polyphenols at lower concentrations induced AI-2 synthesis; however, AI-2 synthesis was significantly inhibited at higher concentrations (300 μg/ml). Food additives inhibited the adhesion of mixed bacteria on stainless steel chips and increased the sensitivity of the mixed biofilm to disinfectants. In conclusion, citral, cinnamaldehyde, and tea polyphenols had strong inhibitory effects on mixed biofilm formation and also enhanced the effect of disinfectant on mixed biofilm formation. This study provides a scientific basis for the application of natural food additives to control biofilm formation of foodborne bacteria.
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RSC Advances
·
2017
·Vol. 7
·期 87
·55141-55147
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IF 3.108
Miao-Lin Duan,Yan-Mei Huang,Song-Song Wu,Guo-Qiang Li,Shu-Ying Wang,Ming-Hui Chen,Chun Wang,Dao-Feng Liu,Cheng-Wei Liu,Wei-Hua Lai
A pre-concentrated immunochromatographic assay for Salmonella enteritidis (S. enteritidis) detection was developed based on the unique optical and magnetic properties of magnetic nanoparticles (MNPs). The target was easily concentrated from a large volume of sample by using immune MNPs (IMNPs) with superparamagnetism. The results could be directly distinguished using colored IMNPs on test strip. A target elution step was not required in the proposed method. The key parameters, including coupling pH, blocking agents, and amount of IMNPs on the test strip, were optimized. Colloidal gold-based immunochromatographic assay (CG-ICA), MNP-based immunochromatographic assay (MNP-ICA), and MNP-ICA for a large-volume sample (large-volume MNP-ICA) were compared. Under optimal conditions, the sensitivity of large-volume MNP-ICA was 1.95 × 105 CFU mL−1, which was 10 times higher than that of MNP-ICA and 1000 times higher than that of CG-ICA, owing to the pre-concentration from a large sample volume. Moreover, the antibody consumption of large-volume MNP-ICA was lower than that of conventional CG-ICA. The entire detection time of the proposed method was only 30 min. In general, the proposed method may be considered as a rapid, sensitive, and simple screening tool for on-site detection of S. enteritidis as well as other pathogens.
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Foods
·
2026
·Vol. 15
·期 3
·479
·
IF 5.1
Baizhi Cen,Juge Liu,Mengyuan Tan,Bo Wang,Lu Gao,Zhenquan Yang,Genxi Zhang,Tao Zhang,Xuechao Xu
In this study, a silver-based metal–organic framework (Ag-MOF) nanozyme was synthesized for the synergistic eradication of drug-resistantSalmonellaenteritidisin food matrices. Ag-MOF exhibits multiple enzyme-like activities, namely oxidase (OXD)-, peroxidase (POD)-, and superoxide dismutase (SOD)-like activities. It demonstrated excellent antibacterial and antibiofilm activities against erythromycin- and chloramphenicol-resistantS. enteritidisstrains (N29 and P23). Specifically, treatment with 20 mg mL−1Ag-MOF resulted in nearly complete eradication ofS. enteritidisin in vitro suspension assays, including 1 × 107CFU mL−1N29 strain and 6 × 106CFU mL−1P23 strain. Moreover, treatment with 1 mg mL−1Ag-MOF led to 80~90% biofilm inhibition ofS. enteritidis. Mechanistic investigations revealed that Ag-MOF effectively interacted with amino-rich structures on the bacterial surface (such as membrane proteins and peptidoglycan components), generated abundant reactive oxygen species (ROS), released Ag+ions, and depleted intracellular glutathione, which collectively disrupted cell membrane integrity and induced severe leakage of intracellular proteins and nucleic acids. Importantly, Ag-MOF maintained high antibacterial efficacy in complex simulated food matrices (pork, milk, and egg shell). Overall, this study offers key insights into enzyme-mimicking antibacterial materials and a promising strategy to combat multidrug resistant foodborne pathogens.
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Foodborne Pathogens and Disease
·
2024
·
IF 1.9
Haiyang Yan,Shuanglin Liu,Youhua Xian,Qingyan Liu,Yan Zhang,Yuan Yuan
The present study assesses the antibacterial effect of a high-voltage prick electric field (HVPEF) on Salmonella enteritidis on gelatin plates and selected food systems. The results showed that S. enteritidis were susceptible to HVPEF treatment, with a mortality rate of 98% on the gelatin plate at 13 kV for 15 min. The survival rate of S. enteritidis decreased linearly with time and then stabilized after a sharp decline. The morphological change and the loss of internal nucleic acids and proteins suggest that the cell membrane was damaged by HVPEF, appearing more permeable and less fluid. Compared with the untreated group, both reactive oxygen species (ROS) and malondialdehyde increased significantly in the treated groups. Flow cytometry results suggest that DNA was severely damaged, which led to cell death, as shown by the elevated levels of intracellular ROS and lipid peroxidation. The death rate of S. enteritidis on the food surface significantly rose with the increase of HVPEF intensity, reaching 91 ± 1.6% (salmon), 88.2 ± 2.2% (chicken breast), 72.9 ± 2.7% (sausage), and 66.9 ± 1.6% (steak), respectively, at 14 kV for 30 min. HVPEF resulted in no significant change in color or texture; the changes in total viable count and pH were less significant than in the untreated group. Finally, HVPEF treatment led to lower levels of total volatile base nitrogen and thiobarbituric acid reactive substances in chicken breasts, which potentially contributes to longer shelf life.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2024
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IF 4.1
Shuting Jiang,Yifan Wang,Haoran Cheng,Gongliang Zhang,Hongman Hou,Jingran Bi,Shuang Yan,Hongshun Hao
A unique multifunctional photoelectrochemical(PEC) platform for the simultaneous detection and inactivation of Salmonella enteritidis (SE) was proposed. The photoactive material in the platform was a Bi 2 MoO 6 /V 2 CTx composite, and gold nanoparticles were modified to link the aptamer via Au-S bonds. The aptamer was to link the complementary DNA (cDNA) to the electrode surface via base complementarity. In the presence of SE, the aptamer attached to Salmonella enteritidis selectively and then the cDNA was released from the aptamer electrode, the photocurrent signal was amplified, allowing for quantitative detection of Salmonella enteritidis . The linear range of the method was 1.82 × 10 2 CFU/mL to 1.82 × 10 8 CFU/mL based on the specific recognition of Salmonella enteritidis by aptamer. The detection limit was 26 CFU/mL. Furthermore, due to its photoelectrocatalytic action, the PEC platform demonstrated strong antibacterial activity (99.99 %). The new technique was created to detect and inactivate Salmonella enteritidis in a timely and sensitive manner.
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Foods
·
2024
·Vol. 13
·期 22
·3709
·
IF 4.7
Mengjun Hu,Zhuoan Zhou,Chenqi Liu,Zeqiang Zhan,Yan Cui,Shoukui He,Xianming Shi
Two-component systems (TCS) of Salmonella enterica serovar Enteritidis are composed of a histidine kinase and a response regulator (RR) and represent a critical mechanism by which bacteria develop resistance to environmental stress. Here, we characterized the functions of RRs in TCS in the formation of stress tolerance, motility and biofilm using twenty-six S . Enteritidis RR-encoding gene deletion mutants. The viability results unraveled their essential roles in resistance to elevated temperature (GlrR), pH alterations (GlrR, TctD, YedW, ArcA and YehT), high salt (PhoB, BaeR, CpxR, PhoP, UvrY and TctD), oxidative stress (PhoB, YedW, BaeR, ArcA, PhoP, UvrY, PgtA and QseB) and motility (ArcA, GlnG, PgtA, PhoB, UhpA, OmpR, UvrY and QseB) of S . Enteritidis. The results of the crystal violet staining, microscopy observation and Congo red binding assays demonstrated that the absence of ArcA, GlnG, PhoP, OmpR, ZraR or SsrB in S . Enteritidis led to a reduction in biofilms and an impairment in red/dry/rough macrocolony formation, whereas the absence of UvrY exhibited an increase in biofilms and formed a brown/smooth/sticky macrocolony. The results indicated the regulatory effects of these RRs on the production of biofilm matrix, curli fimbriae and cellulose. Our findings yielded insights into the role of TCSs, making them a promising target for combating S . Enteritidis.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2023
·
IF 5.4
Zhuolin Ma,Weiying Xu,Shaoting Li,Siyi Chen,Yuheng Yang,Zefeng Li,Tong Xing,Zepeng Zhao,Dongping Hou,Qingqing Li,Ziying Lu,Hongmei Zhang
Involvement of the transcriptional regulator RpoS in the persistence of viable but non-culturable (VBNC) state has been demonstrated in several species of bacteria. This study investigated the role of the RpoS in the formation and resuscitation of VBNC state in Salmonella enterica serovar Enteritidis CICC 21482 by measuring bacterial survival , morphology, physiological characteristics, and gene expression in wild-type (WT) and rpoS -deletion ( ΔrpoS ) strains during long-term storage in powdered infant formula (PIF). The ΔrpoS strain was produced by allelic exchange using a suicide plasmid. Bacteria were inoculated into PIF for 635-day storage. Survival, morphology, intracellular reactive oxygen species (ROS) levels and intercellular quorum sensing autoinducer-2 (AI-2) contents were regularly measured. Resuscitation assays were conducted after obtaining VBNC cells. Gene expression was measured using real-time quantitative polymerase chain reaction (qPCR). The results showed that RpoS and low temperature conditions were associated with enhanced culturability and recoverability of Salmonella Enteritidis after desiccation storage in low water activity (a w ) PIF. In addition, the synthesis of intracellular ROS and intercellular quorum sensing AI-2 was regulated by RpoS, inducing the formation and resuscitation of VBNC cells. Gene expression of soxS , katG and relA was found strongly associated with RpoS. Due to the lack of RpoS factor, the ΔrpoS strain could not normally synthesize SoxS, catalase and (p)ppGpp, resulting in its early shift to the VBNC state. This study elucidates the role of rpoS in desiccation stress and the formation and resuscitation mechanism of VBNC cells under desiccation stress. It serves as the basis for preventing and controlling the recovery of pathogenic bacteria in VBNC state in low a w foods.
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TALANTA
·
2024
·
IF 5.6
Haoran Cheng,Yifan Wang,Yirui Zhao,Hongman Hou,Gongliang Zhang,Jingran Bi,Shuang Yan,Hongshun Hao
Salmonella enteritidis ( SE ) is a food-borne pathogens that can cause acute gastroenteritis. With the increasing social attention to food safety, the detection method of SE has attracted wide attention. In response to the demand for efficient detection methods of SE , this study constructed a novel dual-mode photoelectrochemical-electrochemical (PEC-EC) aptamer-based biosensor. The sensor was constructed using Bi 4 NbO 8 Cl/In 2 S 3 heterojunction as the electrode substrate material, the hybridization chain reaction (HCR) and dye sensitization were used as the signal amplification strategies. Bi 4 NbO 8 Cl/In 2 S 3 heterojunction could provide an excellent initial photocurrent response for the sensing platform, and the HCR was opened by the end of complementary DNA (cDNA) and generated an ultra-long DNA double-stranded (dsDNA) “super structure” on the surface of the electrode, which could be embedded with a large number of methylene blue (MB) as the bifunctional probes. Thus, dual-mode output was achieved via the PEC and EC activity of MB. Under the optimized conditions, the PEC and EC signal responses of the system were linear to the logarithm of SE concentration in a range from 1.5 × 10 2 CFU/mL to 1.5 × 10 7 CFU/mL. The detection limits were found to be 12.9 CFU/mL and 12.3 CFU/mL using the PEC and EC methods, respectively. The constructed dual-mode biosensor exhibited good performance for real sample analysis, and demonstrated great application potential in the field of SE rapid detection. Moreover, this dual-mode detection strategy provided more accurate and reliable results than the single-mode output.
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