Marine Drugs
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2021
·Vol. 19
·期 9
·485
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IF 5.118
Ying Yan,Zhoumin Niu,Boyang Wang,Shangge Zhao,Chao Sun,Yuting Wu,Yuying Li,Hao Ying,Hongbing Liu
Dysregulation of cholesterol homeostasis is a major risk factor of atherosclerosis, which can lead to serious health problems, including heart attack and stroke. Liver X receptor (LXR) α and β are transcription factors belonging to the nuclear receptor superfamily, which play important roles in cholesterol homeostasis. Selectively activating LXRβ provides a promising strategy for the treatment of atherosclerosis. Here, we employed atherosclerotic apoE-knockout mice to evaluate the effects of saringosterol, a phytosterol with potent and selective action for LXRβ, which we identified previously in edible marine seaweedSargassum fusiforme. We found that saringosterol treatment reduced the atherosclerotic plaque burden without having undesirable adverse hepatic effects in apoE-deficient mice fed an atherogenic diet. Meanwhile, reduced serum levels of cholesterol, accompanied by altered expression of LXR-regulated genes involved in cholesterol absorption, transport, efflux, excretion, and elimination, were observed in apoE-knockout mice after saringosterol treatment. Together, our study not only establishes saringosterol as an effective cholesterol-lowering and anti-atherogenic phytosterol but also provides insights into the underlying mechanism.Keywords:saringosterol;liver X receptor;cholesterol metabolism;atherosclerosis;Sargassum fusiforme
研究领域:
药理学
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心血管疾病
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海洋生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Yang Cheng,Luthuli Sibusiso,Lingfeng Hou,Huijing Jiang,Peichao Chen,Xu Zhang,Mingjiang Wu,Haibin Tong
Diabetes is a complicated endocrine and metabolic disorder, which has become an epidemic health issue worldwide. Fucoidan is extensively distributed in the brown algae and several marine invertebrates exhibiting diverse biological activities. In the present study, the physicochemical property of Sargassum fusiforme fucoidan (SFF) and its effects on streptozotocin (STZ)-induced diabetic mice and gut microbiota were investigated. Diabetes mice not only showed abnormal blood glucose, but also accompanied by multiple symptoms, such as gradual emaciation, decreased body weight, increased food and water intake. Compared with diabetic mice after 6-week treatment, administration of SFF significantly decreased the fasting blood glucose, diet and water intake. Furthermore, SFF attenuated the pathological change in the heart and liver, improved the liver function, and suppressed oxidative stress in STZ-induced diabetic mice. Simultaneously, SFF significantly altered the gut microbiota in the faeces of diabetic mice, decreased the relative abundances of the diabetes-related intestinal bacteria, which is a potential mechanism for relieving the symptoms of diabetes. Therefore, SFF might be considered as one of the promising complementary and alternative medicines for the management of diabetes mellitus in future.
研究领域:
海洋生物学
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药理学
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微生物学
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内分泌学
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PubMed
高影响因子
Chinese Herbal Medicines
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2025
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IF 8.9
Yue Zhu,Yuting Ai,Jianfu Zhou,Xiaozhuan Jia,Shuying Liu,Bo Zhu,Yimin Qin,Jianping Jiang,Zhenzhong Yang
Objective Sargassum fusiforme (SF), a brown algae, is a marine traditional Chinese medicine with nutritional value. However, studies on the bioactive components in SF are limited. The aim of this study is to investigate phytochemical and biological properties of SF from different geographical origins and botanical parts, as well as to explore bioactive components within SF. Methods SF from different origins and botanical parts were collected for integrated phytochemical analyses using ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF MS), mass spectral molecular networking and liquid chromatography-single quadrupole mass spectrometry (LC-MSD), etc., followed by the systematic chemical profile evaluation. Subsequently, the glycolipid metabolism regulation and anticoagulation of SF were examined. Furthermore, the overall information acquired was assembled using multivariate analysis and machine learning to discover bioactive components. Results A total of 123 compounds in SF were identified with the assistance of mass spectral molecular networking. The variations in the content of amino acids, fucoidan, alginate, phlorotannin, alkaloids, terpenoids, etc. were observed in SF from different origins and botanical parts. SF possessed the hypolipidemic, hypoglycemic and anticoagulant effects, and SF from Dongtou, Wenzhou, Zhejiang, was outstanding among origins in both phytochemical and biological evaluations. A heterogeneous information ensemble approach was adopted to discover the bioactive components in SF, namely 3-indoleacrylic acid, phlorotannin and fucoidan. Conclusion This study evaluated the phytochemical and biological properties of SF, revealed the bioactive components using heterogeneous information and provided a reference for the rational consumption and further development of SF.
研究领域:
植物化学
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海洋药理学
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分析化学
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生物信息学
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营养科学
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Chuanhai Tu,Tingfu Yu,Shaobiao Feng,Ning Xu,Aneth Massawe,Shanshan Shui,Bin Zhang
In this study, a novel kombucha-fermented Sargassum fusiforme beverage was prepared, and physicochemical properties, volatile flavor compounds, and microbial communities during the beverage fermentation were investigated. The results indicated that after fermentation, the pH decreased to 2.77 ± 0.05, titratable acid content increased to 4.42 ± 0.20 mg/mL, organic acids content increased and acetic acid became the main organic acid, values for total sugar content decreased and the reducing sugar content increased during fermentation. The clearance rates of DPPH radicals, hydroxyl radicals, superoxide anion radicals, and ABTS radicals increased after fermentation. In addition, the results of high-throughput sequencing showed that Zygosaccharomyces was the main fungi, and Komagataeibacter and Acetobacter were the main genera of bacteria in kombucha-fermented S. fusiforme beverage. Gas chromatography-ion migration spectrometry results showed that ethanol, acetic acid, acetone, and ethyl acetate were the main volatile flavor compounds. The correlation results between the dominant microbial community and the major volatile flavor compounds indicated that Pichia , Aspergillus , Zygosaccharomyces , Acetobacter, and Ralstonia had a positive effect on the flavor formation of beverages, which provided the direction for the later development and accurate screening of flavorous functional microorganisms.
研究领域:
食品科学
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微生物学
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化学
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高影响因子
Journal of Ocean University of China
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2016
·Vol. 15
·期 3
·533-540
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IF 0.509
Fu Zhifei,Li Haihua,Liu Hongbing,Hu Shuman,Li Yueying,Wang Mengxue,Guan Huashi
The response surface methodology (RSM) combined with bioassays was employed to optimize the extraction process of crude fucose-containing sulphated polysaccharides (cFCSP) from Sargassum fusiforme . The central composite design (CCD) was used with four variables, five levels, and four responses. The four variables were pH value of hydrochloric acid solution, extraction temperature (°C), ratio of liquid to raw material (mL g −1 ), and extraction time (h), respectively. Chemical and bioassay indices were used in combination as the response parameters, which included the yield of cFCSP, fucose content, proliferation rate of spleen cells, and lipopolysaccharide-induced proliferation of splenocytes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, and examined using the appropriate statistical methods. The best extraction conditions were as follows: the pH value of hydrochloric acid solution was 3.50; the extraction temperature was 100°C; the ratio of liquid to raw material was 15.00 mL g −1 and the extraction time was 2.50 h. The experimental yield was close to the predicted from the model. The extract could promote spleen lymphocyte proliferation, especially the lipopolysaccharide-induced lymphocyte proliferation in vitro, which suggested that its immunomodulatory effect on B lymphocytes. Therefore, cFCSP extracted from S. fusiforme could be utilized as an immunostimulant in functional foods and pharmaceutical industry in future.
研究领域:
海洋生物学
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药理学
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食品科学
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DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
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2021
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IF 6.025
Qiuhong Kong,Ruifen Zhang,Lijun You,Yongxuan Ma,Lan Liao,Sandra Pedisić
In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction. The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated. Results showed that the contents of total carbohydrate , protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively. It mainly consisted of fucose glucose and galactose , with molecular weight of 255.83 kDa. UV spectrum, FTIR , SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape. After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly ( p < 0.05), utilization of carbohydrate was 53.17%. The contents of total SCFAs increased by 10.77 times. Moreover, SFP fermentation could change obviously the microbiota composition. It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group) , Phascolarctobacterium (increased by 88.06%) , Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%) , and Lactobacillus (increased by 400.00%) , decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level. These results showed that SFP could be utilized by microbiota in human feces, and may have the potential to improve intestinal health.
研究领域:
食品科学
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微生物学
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PubMed
高影响因子
FOOD CHEMISTRY
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2025
·
IF 9.8
Jiwei Zhu,Yi Guan,Cuirong Luan,Xiaohan Lin,Zhiyu Li,Shuji Liu,Yijing Wu,Jie Yang
Frozen aquatic products quality degrades primarily due to ice crystal formation. This study developed a composite cryoprotectant (CC) consisting of marine Sargassum fusiforme polysaccharide (SFP) for frozen large yellow croaker fillets. The optimal formulation of CC was 1 % SFP, 0.125 % tea polyphenols, and 2.25 % food-grade phosphate. The fillets treated with CC and stored at −18 °C for 80 days showed higher salt-soluble protein content, Ca 2+ -ATPase activity, total sulfhydryl content, apparent viscosity, and a more intact microstructure compared to control group. TCA-soluble peptides and thiobarbituric acid value were lower, indicating improved myofibrillar protein stability and overall fillet quality . The treatment enhanced water retention and distribution characteristics, demonstrating the high cryoprotection efficiency of the SFP-based CC. This novel cryoprotectant shows effects comparable to, or even exceeding, commercial cryoprotectant in mitigating ice crystal-induced damage, making it a promising solution for frozen aquatic products storage.
研究领域:
食品科学
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水产加工
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冷冻生物学
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蛋白质稳定性
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食品质量
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DOI
PubMed
高影响因子
Frontiers in Nutrition
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2024
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IF 4
Jiang Long,Song Chen,Ai Chunqing,Wen Chengrong,Song Shuang
The present study demonstrated the digestion behavior and fermentation characteristics of a sulfated polysaccharide from Sargassum fusiforme (SFSP) in the simulated digestion tract environment. The results showed that the molecular weight of two components in SFSP could not be changed by simulated digestion, and no free monosaccharide was produced. This indicates that most of SFSP can reach the colon as prototypes. During the fermentation with human intestinal flora in vitro, the higher-molecular-weight component of SFSP was utilized, the total sugar content decreased by 16%, the reducing sugar content increased, and the galactose content in monosaccharide composition decreased relatively. This indicates that SFSP can be selectively utilized by human intestinal flora. At the same time, SFSP also changed the structure of intestinal flora. Compared with the blank group, SFSP significantly increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes. At the genus level, the abundances of Bacteroides and Megamonas increased, while the abundances of Shigella, Klebsiella, and Collinsella decreased. Moreover, the concentrations of total short-chain fatty acids (SCFAs), acetic, propionic and n-butyric acids significantly increased compared to the blank group. SFSP could down-regulate the contents of trimethylamine, piperidone and secondary bile acid in fermentation broth. The contents of nicotinic acid, pantothenic acid and other organic acids were increased. Therefore, SFSP shows significant potential to regulate gut microbiota and promote human health.
研究领域:
食品科学
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微生物学
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营养学
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PubMed
高影响因子
Marine Drugs
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2020
·Vol. 18
·期 9
·444
·
IF 4.073
Bin Wei,Qi-Wu Zhong,Song-Ze Ke,Tao-Shun Zhou,Qiao-Li Xu,Si-Jia Wang,Jian-Wei Chen,Hua-Wei Zhang,Wei-Hua Jin,Hong Wang
A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention. In this study, five polysaccharides were prepared fromSargassumfusiformeand their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated. The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and fourSargassumfusiformepolysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia. Sf-1 and Sf-A could also significantly prevent HFD-induced weight gain. Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance ofOscillospira,Mucispirillum, andClostridiales. Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance ofMucispinllumandOscillospira. Receiver operating characteristic analysis indicated thatMucispinllumandOscillospiraexhibited good discriminatory power (AUC = 0.745–0.833) in the prediction of fasting hypoglycemia. Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles ofMucispinllumandOscillospirain the biological activity of SFPs.Keywords:Sargassum fusiformepolysaccharides;high-fat diet;gut microbiota;early fasting hypoglycemia
研究领域:
营养学
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微生物学
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代谢病研究
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Siya Wu,Xu Zhang,Jian Liu,Jianxi Song,Ping Yu,Peichao Chen,Zhiyong Liao,Mingjiang Wu,Haibin Tong
P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases. It is reported that brown algal fucoidans can antagonize P-selectin function. However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated. In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions. Their physicochemical characterization was determined by chemical methods, HPLC and FT-IR. The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber. Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions. Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion.
研究领域:
海洋生物学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Xiang Li,Shijun Xin,Xiaoqun Zheng,Liqin Lou,Shiqing Ye,Shengkai Li,Qilong Wu,Qingyong Ding,Ling Ji,Chunrong Nan,Yongliang Lou
Fucoidan has many biological activities, including the inhibitory effect on the development of various cancer types. This study showed that lipopolysaccharide-induced inflammation in FHC cells (normal human colonic epithelial cells) could be reversed using fucoidan at different concentrations. The fucoidan-induced anti-inflammatory effect was also confirmed through in vivo experiments in mice. Compared to the mice of the model group, the ratio of Firmicutes/Bacteroidetes in feces increased and the diversity of gut microbial composition was restored in mice after fucoidan intervention. In colorectal cancer (CRC) cells DLD-1 and SW480, fucoidan inhibited cell proliferation and promoted cell apoptosis. It also blocked the cell cycle of DLD-1 and SW480 at the G0/G1 phase. The animal model of inflammation-related CRC showed that the incidence of tumors in mice was significantly reduced by fucoidan intervention. Furthermore, the administration of fucoidan decreased the expression levels of inflammatory factors such as TNF-α IL-6 and IL-1β in the colonic tissues. Therefore, fucoidan can effectively prevent the development of colitis-associated CRC.
研究领域:
癌症生物学
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免疫学
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胃肠病学
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微生物学
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药理学
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PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 11
·3265
·
IF 4.412
Yuhao Sun,Xiaolin Chen,Hong Liu,Song Liu,Huahua Yu,Xueqin Wang,Yukun Qin,Pengcheng Li
Avian leukosis virus subgroup J (ALV-J) is an immunosuppressive virus which has caused heavy losses to the poultry breeding industry. Currently, there is no effective medicine to treat this virus. In our previous experiments, the low-molecular-weightSargassum fusiformepolysaccharide (SFP) was proven to possess antiviral activity against ALV-J, but its function was limited to the virus adsorption stage. In order to improve the antiviral activity of the SFP, in this study, three new SFP long-chain alkyl group nanomicelles (SFP-C12M, SFP-C14M and SFP-C16M) were prepared. The nanomicelles were characterized according to their physical and chemical properties. The nanomicelles were characterized by particle size, zeta potential, polydispersity index, critical micelle concentration and morphology. The results showed the particle sizes of the three nanomicelles were all approximately 200 nm and SFP-C14M and SFP-C16M were more stable than SFP-C12M. The newly prepared nanomicelles exhibited a better anti-ALV-J activity than the SFP, with SFP-C16M exhibiting the best antiviral effects in both the virus adsorption stage and the replication stage. The results of the giant unilamellar vesicle exposure experiment demonstrated that the new virucidal effect of the nanomicelles might be caused by damage to the phospholipid membrane of ALV-J. This study provides a potential idea for ALV-J prevention and development of other antiviral drugs.Keywords:Sargassum fusiforme;polysaccharide;long-chain alkyl group;antiviral activity;ALV-J
研究领域:
兽医学
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药理学
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分子生物学
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纳米技术
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DOI
PubMed
高影响因子
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