HARMFUL ALGAE
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2018
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IF 4.138
Miaomiao Zhao,Xinyi Chen,Ning Ma,Qiuyue Zhang,Dong Qu,Ming Li
To form Microcystis blooms, Microcystis must be competitively dominant to other phytoplankton species to produce enough biomass. The aim of this study was to determine the competition mechanisms between Microcystis aeruginosa and Scenedesmus obliquus . M. aeruginosa and S. obliquus were separately cultured in the filtrate of mono and mixed cultures of M. aeruginosa and S. obliquus with varying treatments concerning N, P and iron availability. The inhibition rate for M. aeruginosa was 20–31% when cultured in mono and mixed culture filtrates enriched with N and P, but this rate was reduced to −15 to 19% when cultured in filtrates enriched with N, P and iron. The inhibition rate for M. aeruginosa decreased from 80 to 100% to 11–39% in dialytic filtrates. However, there were no differences in inhibition rate for S. obliquus, regardless of filtrate or dialytic filtrate treatments. The potential allelochemical, 2-butyl-octanol (0.144 mg L −1 ), was found to have little inhibitory effect to M. aeruginosa or S. obliquus . Thus, previously reported allelopathy to Microcystis would be overestimated. We also report a new mechanism in phytoplankton competition in this study. The phytoplankton secreted humic acid-like substances that can reduce the bioavailability of iron, resulting in the inhibition of other phytoplankton.
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Journal of Cleaner Production
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2026
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IF 10.7
An'an Xiao,Lu Xiong,Shanshan Shen,Xinran Li,Shulei Yang,Fangyan Shen,Qian Hu,Fangni Lin,Lian Huang,Jinquan Liu,Zhaohui Zhang,Hui Wang
Physiological & transcriptomic responses of Scenedesmus obliquus to 0.01–20.0 mg/L BeSO 4 were studied. • S. obliquus accumulated 3.53 mg of BeSO 4 with 88.1% removal efficiency at 20.0 mg/L. • Genes related to photosynthesis (44), stress response (93), and nitrogen metabolism (46) were upregulated. • Eighty-six upregulated ion-binding and transport genes promoted Be 2+ bioaccumulation. • S.obliquus shows promise for bioremediation of beryllium-contaminated aquatic environments. Beryllium (Be) is a widely utilized industrial metal with favorable physicochemical properties but poses severe chronic toxicity to aquatic organisms and humans, mandating safe and efficient removal from aqueous solutions. Current algal biosorption research remains limited for Be 2+ separation and underlying molecular mechanisms. In this study, the green microalgae Scenedesmus obliquus was exposed to varying concentrations of BeSO 4 (0.01, 0.1, 1.0, 10.0, and 20.0 mg/L), and its growth, photosynthesis, oxidative stress response, Be 2+ removal performance, and transcriptomic responses were investigated. Short-term exposure to BeSO 4 had negligible effects on microalgal cell growth, chlorophyll a content and antioxidant system, whereas high dose (20.0 mg/L) or prolonged exposure (4 days) significantly inhibited these processes. The total bioaccumulation amount of beryllium increased continuously with elevating BeSO 4 concentrations, ranging from 0.002 mg to 3.53 mg. Although the beryllium removal efficiency was relatively lower under 1 mg/L BeSO 4 treatment (40.02%), it exhibited a steady increase with rising BeSO 4 concentration and ultimately reached 88.14% at 20.0 mg/L BeSO 4 on day 1. Transcriptomic analysis revealed that most genes associated with photosynthesis (44 transcripts), antioxidant system (33), stress response (60), nitrogen metabolism (46), endocytosis (38), and ion binding and transport (86) were upregulated, contributing to the high tolerance and total bioaccumulation of S. obliquus to beryllium ions. These findings revealed physiological and molecular mechanisms of beryllium tolerance and bioaccumulation in S. obliquus , providing a scientific foundation for the treatment of beryllium-containing wastewater and aquatic bioremediation. Download: Download high-res image (300KB) Download: Download full-size image
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SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Xuanhe Jia,Yueqiang Pan,Xuexia Zhu
Driven by anthropogenic activities, freshwater salinization has become an emerging global environmental issue . Recent studies indicate that salinization increases the mobility of heavy metals in soil and causes higher flux into surface waterbodies. The present study assessed the combined effects of salinization (0, 3, 6 PSU) and the heavy metal Cd 2+ (0, 0.2, 0.4 mg L −1 ) on the anti-grazing colony formation and population growth of Scenedesmus obliquus , a common freshwater alga. The results showed that the increase in salinity promoted colony formation of S. obliquus with or without the presence of grazing cues and, in contrast, Cd 2+ contamination depressed the defensive colony formation of S. obliquus to Daphnia filtrate. The increase in both salinity and Cd 2+ concentration depressed the population growth of S. obliquus , including impaired photosynthesis and a decreased population growth rate. Salinization moderated the negative effects of Cd 2+ on defensive colony formation of S. obliquus , suggesting increased absorption of Cd 2+ ions by a thicker outer layer of the algal cell wall under saltier conditions. As a result, larger defensive colonies of S. obliquus under freshwater salinization may cause higher bioaccumulation of heavy metals by algal cells and heavier influence on zooplankton. This study provides evidence that freshwater salinization could interfere with plankton interactions by affecting algal defense and growth, which may lead to bottom-up cascading effects on freshwater food webs.
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COMPUTERS AND ELECTRONICS IN AGRICULTURE
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2021
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IF 5.565
Bingquan Chu,Kai Chen,Xiaoxiao Pan,Qianying Wu,Shiwang Liu,Jinyan Gong,Xiaoli Li
Microalgae lipid and its degree of fatty acid unsaturation (DU) have the ability to undergo programmatic changes in response to different environmental and culture conditions. A real-time and reliable approach to monitor the change patterns of lipid-accumulation and DU is crucial for microalgal culture and bioprocess control. In this study, a fast visual and nondestructive method based on visible/short-wave near-infrared hyperspectral imaging (V/SNIR HSI) at 400–1000 nm region was developed to investigate the dynamic changes and content distribution of lipids and DU of Scenedesmus obliquus treated by nitrogen-low stress (0.3 g/L NaNO 3 , NL) and nitrogen-free stress (0 g/L NaNO 3 , NF). After collecting the average spectra from the region of interests (ROIs), the competitive adaptive reweighted sampling (CARS) algorithm and the successive projection algorithm (SPA) were applied to pick effective wavebands from the spectral data for the establishment of the lipid concentration and DU estimation models including partial least squares regression (PLSR) and multiple linear regression (MLR) . The prediction results show the CARS-PLSR (R p = 0.9762, RMSE p = 0.0143) and CARS-MLR (R p = 0.9477, RMSE p = 0.5718) performed the best for lipids and DU of S. obliquus , respectively. Based on the optimal models, the spatial distribution of lipid abundance and DU in liquid suspension were visualized, which could be helpful in exploring the dynamic process of lipid synthesis and DU of S. obliquus. The overall results show that V/SNIR HSI is a promising technique for fast and noninvasively monitoring the lipid-accumulation and its characteristics i.e. DU in situ, and is expected to be applied for efficient and high-quality lipid production in microalgae industries.
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ENVIRONMENTAL POLLUTION
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2020
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IF 6.793
Xuexia Zhu,Zeshuang Wang,Yunfei Sun,Lei Gu,Lu Zhang,Jun Wang,Yuan Huang,Zhou Yang
The ecotoxicology of surfactants is attracting wide attention due to the rapidly expanding global application. As interspecific relationships play one of the central roles in structuring biological communities, it is necessary to take it into risk assessments on surfactants. With this aim, our study investigated the interference of three common surfactants on the inducible defense of a freshwater phytoplankton Scenedesmus obliquus . Nonlethal environmentally relevant concentrations (10 and 100 μg L −1 ) of several surfactants were set up. Results showed that growth and photosynthetic efficiency of Scenedesmus were inhibited during first 96 h, but recovered in the later stage. Surfactants interfered inducible defense of Scenedesmus against Daphnia grazing, and the interference was related to chemical characteristics of surfactants. The anionic surfactant sodium dodecyl sulfate (SDS) enhanced the colony formation even without grazing cues, whereas fewer defensive colonies were formed under the effects of cationic surfactant benzalkonium bromide (BZK) and nonionic surfactant polyoxyethylene (40) nonylphenol ether (NPE). These findings highlighted the sensitivity of grazer-induced morphological defense of Scenedesmus to surfactants even at nonlethal concentrations, which potentially affects the energy and information flow between trophic levels . This study appeals for more attention to take interspecific relationships into consideration in assessing the potential ecological risk of pollutants.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Jie-Ru Jiang,Zhi-Feng Chen,Xiao-Liang Liao,Qian-Yi Liu,Jia-Ming Zhou,Shi-Ping Ou,Zongwei Cai
Tire wear particles (TWPs) are increasingly being found in the aquatic environment. However, there is limited information available on the environmental consequences of TWP constituents that may be release into water. In this study, TWP leachate samples were obtained by immersing TWPs in ultrapure water. Using high-resolution mass spectrometry and toxicity identification, we identified potentially toxic organic substances in the TWP leachates. Additionally, we investigated their toxicity and underlying mechanisms. Through our established workflow, we structurally identified 13 substances using reference standards. The median effective concentration (EC 50 ) of TWP leachates on Scenedesmus obliquus growth was comparable to that of simulated TWP leachates prepared with consistent concentrations of the 13 identified substances, indicating their dominance in the toxicity of TWP leachates. Among these substances, cyclic amines (EC 50 : 1.04–3.65 mg/L) were found to be toxic to S. obliquus . We observed significant differential metabolites in TWP leachate-exposed S. obliquus , primarily associated with linoleic acid metabolism and purine metabolism. Oxidative stress was identified as a crucial factor in algal growth inhibition. Our findings shed light on the risk posed by TWP leachable substances to aquatic organisms.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
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IF 4.527
Kun Yan,Yanhua Liu,Qiulian Yang,Weixia Liu,Ruixin Guo,Jinhong Sui,Zhengyu Yan,Jianqiu Chen
Quantum dots (QDs), as a kind of novel nanomaterial , have the extensive applications in various fields, inevitably leading to increasing risks for the ecological environment. The mobilization of cadmium including metal smelting and subsequent machining for multifarious applications has caused the release of cadmium element into the environment. In this study, we evaluated the potential toxicity of a novel nanoparticle material CdSe QDs, using two green algae Chlorella pyrenoidosa and Scenedesmus obliquus . The impact of CdSe QDs and cadmium ions on algae and the sensitivity of the two algae on target compounds were also considered and compared. Our results showed the algal growth rates and chlorophyll content decreased with increasing exposure concentrations and durations. Moreover, the glutathione levels were decreased while the activities of superoxide dismutase increased, exhibiting their pivotal functions in defeating toxic stress. The increment of malondialdehyde levels revealed that the stresses of CdSe QDs and cadmium ions were contributed to the occurrence of oxidative damage. Our study also indicated that the impact of CdSe QDs was stronger than that of cadmium nitrate and the algal response was also species-specific. In addition, the TEM photographs of the algal ultrastructure showed the presence of surface attachment and uptake of QDs.
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
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2019
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IF 3.061
Yinghan Liu,Zhuang Wang,Se Wang,Hao Fang,Nan Ye,Degao Wang
The importance of attention to unravel the interaction of nano-plastic particles (NPs) with natural acidic organic polymer (NAOP) in freshwater environment should not be neglected. However, toxicological data available for the interaction between NPs and NAOP remain limited. Here, we investigate the toxicological effects of three model polystyrene (PS) NPs with different functional groups (unmodified, amino- and carboxyl-modified PS NPs) on two freshwater organisms of different trophic levels ( Scenedesmus obliquus and Danio rerio ) in the absence and presence of two classes of NAOP, namely fulvic acid and humic acid. The NAOP interaction with the NPs is shown to alter oxidative stress and disturb membrane function in S. obliquus cells to a certain extent. Combined oxidative stress responses to the NPs and NAOP in D. rerio as a function of their mixture levels showed inhibition, alleviation, and reinforce. Changes in cellular oxidative stress and membrane function depended on the concentration and types of both NPs and NAOP. Furthermore, the characterization parameters of the NPs were important for the explanation of the ecotoxicological mechanism of the NPs in the presence of NAOP. Our findings emphasized the critical role of NAOP in the fate and toxicity of plastic particles in freshwater environment.
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BIORESOURCE TECHNOLOGY
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2019
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IF 6.669
Yuxi Liu,Wenbiao Jin,Xu Zhou,Song-Fang Han,Renjie Tu,Xiaochi Feng,Paul D. Jensen,Qilin Wang
In this work, a novel flocculation process by using nano-Fe 3 O 4 coated with polyethyleneimine (Fe 3 O 4 @PEI) as magnetic seeds was developed to harvest the microalgae cultivated in urban sewage. Experiment results indicated that the harvest efficiency of Chlorella pyrenoidosa (0.5 g/L) was 98.92 ± 0.41% under the optimal conditions of Fe 3 O 4 @PEI dose of 20 mL/L, flocculation time of 20 min, and stirring speed of 800 rpm (3 min), while that of Scenedesmus obliquus (0.4 g/L) was 98.45 ± 0.35% under a Fe 3 O 4 @PEI dose of 16 mL/L, flocculation time of 15 min, and stirring speed of 730 rpm (3 min). Moreover, the process did not reduce the lipid content of microalgae and quality of biodiesel. After microalgae harvest, Fe 3 O 4 @PEI could be recovered by ultrasonication, re-wrapped with polyethyleneimine and reused to reduce operational cost.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Junwei Huang,Zilin Zhu,Ruomu Chen,Dandan Pan,Qing X. Li,Xiangwei Wu
Ibuprofen, a widely used nonsteroidal anti-inflammatory drug, contaminates agricultural products and potentially threatens human health due to its frequent detection and poor biodegradability. Microbial metabolism dominates the elimination of residual ibuprofen in the environment. In mineral salt medium at pH 6 with 5 mM glucose, Streptomyces sp. D218 transformed ibuprofen concentrations ranging from 0.05 to 0.40 mM in 24 h. The optimal temperature, pH, and initial OD600 nm for ibuprofen transformation by strain D218 were 25–37 °C, 5.0–6.0, and 1.0–1.5, respectively. Strain D218 could simultaneously transform ibuprofen into the intermediates 2-hydroxyibuprofen and ibuprofen amide (IBUA). The two intermediates were further metabolized to 2-hydroxyibuprofen amide (2HIBUA), thus relieving the growth inhibition of ibuprofen in Scenedesmus obliquus. This is the first complete pathway reported for the detoxification of ibuprofen transformation by a Gram-positive strain. These findings further our understanding of the microbial catabolism of the IBU.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2024
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IF 6.1
Xibo Lu,Qi Yu,Seyed Ali Johari,Zhuang Wang
Even though microplastics (MPs) and graphene nanomaterials (GNMs) have demonstrated individual toxicity towards aquatic organisms , the knowledge gap lies in the lack of understanding regarding their combined toxicity. The difference between the combined toxicity of MPs and GNMs, in contrast to their individual toxicities, and furthermore, the elucidation of the mechanism of this combined toxicity are scientific questions that remain to be addressed. In this study, we examined the individual and combined toxicity of three polystyrene microplastics (MPs) with different functional groups—unmodified, carboxyl-modified (COOH-), and amino-modified (NH 2 -) MPs—in combination with reduced graphene oxide (RGO) on the freshwater microalga Scenedesmus obliquus . More importantly, we explored the cellular and molecular mechanisms responsible for the observed toxicity. The results indicated that the growth inhibition toxicity of RGO, either alone or in combination with the three MPs, against S. obliquus increased gradually with higher particle concentrations. The mitigating effect of MPs-NH 2 on RGO-induced toxicity was most significant at a higher concentration, surpassing the effect of unmodified MPs. However, the MPs-COOH did not exhibit a substantial impact on the toxicity of RGO. Unmodified MPs and MPs-COOH aggravated the inhibition effects of RGO on the cell membrane integrity and oxidative stress-related biomarkers. Additionally, MPs-COOH exhibited a stronger inhibition effect on RGO-induced biomarkers compared to unmodified MPs. In contrast, the MPs-NH 2 alleviated the inhibition effect of RGO on the biomarkers. Furthermore, the presence of differently functionalized MPs did not significantly affect RGO-induced oxidative stress and photosynthesis-related gene expression in S. obliquus , indicating a limited ability to modulate RGO genotoxicity at the molecular level. These findings can offer a more accurate understanding of the combined risks posed by these micro- and nano-materials and assist in designing more effective mitigation strategies.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2020
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IF 6.551
Shang Li,Yi Tao,Guo-Hua Dao,Hong-Ying Hu
The risk of harmful algal blooms (HABs) in the water recharged with reclaimed water is a bottleneck for water reuse . The suppression effects and mechanisms of the combination of UV-C and berberine on Microcystis aeruginosa and Scenedesmus obliquus in reclaimed water were investigated. Mono UV-C irradiation at 75 mJ cm −2 could suppress the growth of M. aeruginosa for 7 d and that at 90 mJ cm −2 could suppress the growth of S. obliquus for 5 d. UV-C irradiation combined with 0.2–2 mg L −1 berberine lengthened the inhibition period of M. aeruginosa to 10- > 22 d and that of S. obliquus to 7- > 22 d and induced more rapid lethal effects on the harmful microalgal cells, in significant synergetic patterns. The combination of UV-C and berberine suppressed total, intracellular and extracellular microcystin-LR (MC-LR) more effectively and decreased the MC-LR quota significantly, which further reduced the risks of microcystin production and release. Furthermore, synergetic mechanisms of the combined treatments were systematically investigated from the aspects of photosynthetic system (photosynthetic activity and pigments), metabolic activity (ATP and membrane potential), oxidation stress (reactive oxygen species (ROS) and glutathione (GSH)), and apoptosis-like cell death (phosphatidylserine (PS) ectropion, caspase-3 activity and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive rate). The combination treatment provided a joint attack of UV-C and berberine on photosynthetic transport chain of photosynthetic system II (PS II), and a synergetic pathway to achieve more severe disruptions in energy metabolism as well as aggravated oxidative stress . The accumulated ROS enhanced increases in programmed cell death (PCD) indicators of both microalgal species, which contributed to the enhancement effects on growth suppression. The results showed that the combination treatment achieved lower dose requirements of both UV-C irradiation and berberine for inducing the same inhibition effects on microalgal cells, which was promising to be applied in the HABs control of reclaimed water.
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