CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2023
·
IF 3.9
Tiantian Tao,Beibei Li,Shengzhe Jia,Mingyang Chen,Zhenguo Gao,Junbo Gong
The utilisation of nucleation seeds, coupling nucleation and growth during crystallization and realistic continuum are currently relatively robust, reliable and scalable methods for protein crystallization optimization. Here, we design a segmented crystallization method, in which combines the characteristics of the Taylor vortex enhanced lysozyme nucleation stage. Different crystallizer are used for the growth segment so that the nucleation segment and the growth segment are organically combined during lysozyme crystallization. During the crystal growth stage, the crystallization properties of the Couette-Taylor (CT) crystallizer, mixing Tank (MT) crystallizer and tubular crystallizer were evaluated. The experimental results emphasize the importance of shear rate, temperature, and fluid velocity in the design of segment crystallization methods to obtain products with complete crystal morphology and uniform size distribution. This strategy successfully expanded the lysozyme crystallization from the original 10 mL scale to 200 mL, realized the continuous process, and providing a promising strategy for the design and operation of continuous SFC to produce high value protein crystals in the future.
研究领域:
化学工程
·
生物技术
·
晶体学
·
蛋白质科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Qingqiang Wang,Quan Li,Xuancheng Liu,Xunli Zhang,Caipeng Wang,Ying Chen,Nan Jin,Yuchao Zhao
The wettability of the channel walls plays a crucial role in determining the flow hydrodynamics and mass transfer characteristics of gas–liquid two-phase flows in microchannel reactors. However, the dynamics of mass transfer coupled with chemical absorption remain to be fully understood. In this study, PMMA-made microchannels were modified to achieve contact angles of 10°, 40° and 70° under optimized conditions, and their effects on gas bubble formation, length, and flow velocity were characterized. This was followed by a quantitative analysis of mass transfer in both CO 2 -MEA and CO 2 -H 2 O systems. It was found that bubble length increased with greater channel wettability and lower liquid-phase flow rate, while also becoming longer with higher gas-phase flow rates. Bubble velocity decreased with increasing channel wettability at any given position along the channel, and in the CO 2 -H 2 O system it remained significantly greater than in the CO 2 -MEA system downstream of the main channel. Based on experimental observations and Higbie’s unsteady state diffusion theory, a novel semi-empirical model was developed to quantitatively characterize the dynamics of gas–liquid mass transfer in slug flow coupled with chemical absorption along microchannels. The calculated values of the overall volumetric liquid phase mass transfer coefficient showed good agreement with the experimental results. This work provides valuable insights for the design and optimization of compact, high-efficiency gas–liquid contactors, which are critical for advancing next-generation CO₂ capture technologies.
研究领域:
化学工程
·
微流体学
·
反应工程
·
分离过程
·
流体动力学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Yanyan Liu,Qiankun Zhao,Jun Yue,Chaoqun Yao,Guangwen Chen
The mass transfer of slug flow, a widely applied flow pattern in microreactors, is still difficult to predict mainly due to the competing nature between convection and diffusion. This work focused on the influence of the mixing mechanism on the mass transfer performance under gas–liquid and liquid–liquid slug flow, in both continuous slugs and dispersed droplets. Colorimetric study with the resazurin oxidation system was implemented, where the mass transfer resistance was constantly located in the aqueous phase. In the hydrophilic glass microreactor, the convection featured by intensive internal circulation and/or inter-slug leakage flow dominated diffusion, leading to nearly-constant k L a along the channel under given flow rates. However, in the hydrophobic PTFE capillary, the stagnant region constituted a significant share in the aqueous droplet, indicating the prominent role of diffusion against convection therein. As a result, k L a values decreased along the main channel length in fixed operating conditions. Accordingly, prediction models were respectively correlated depending on mixing mechanisms. Moreover, mass transfer contributions from the bubble and droplet formation stages were also investigated. This work is expected to shed light on judicious process design and reliable predictions in microreactor operations.
研究领域:
化学工程
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Zhichuang Zuo,Zhicheng He,Yan Ma,Chen Xu,Xuelin Tao,Zimo Ning,Li Gao,Ailainuer Rehemiding,Na Gao,Jinhua Zhao
The slug Vaginulus alte is used as folk medicine in China, but the structure and activities of its galactan components remain to be clarified. Here, the galactan from V. alte (VAG) was purified. The Mw of VAG was determined as ~28.8 kDa. Chemical composition analysis showed that VAG was composed of d -galactose (75 %) and l -galactose (25 %). To elucidate its precise structure, a series of disaccharides and trisaccharides were purified from mild acid hydrolyzed VAG and their structures were characterized by 1D/2D NMR spectroscopy. Based on methylation analysis and structural analysis of oligosaccharides , VAG was elucidated as a highly branched polysaccharide and mainly composed of (1 → 6)- or (1 → 3)-linked β- d -galactose, and distinct (1 → 2)-linked α- l -galactose. The investigation of probiotic effects in vitro revealed that VAG could promote the growth of B. thetaiotaomicron and B. ovatus , while had no effect on the growth of L. acidophilus , L. rhamnosus , B. longum subsp. infantis and B. animalis subsp. lactis , but dVAG-3 with Mw ~1.0 kDa could promote the growth of L. acidophilus . These results will provide insights into specific structures and functions of polysaccharides from the V. alte .
研究领域:
生物化学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2019
·
IF 3.585
Guangxu Cao,Shuang Li,Hezhan Shi,Peidi Yin,Jialing Chen,Huifeng Li,Ying Zhong,Li-Ting Diao,Bin Du
Tubulointerstitial fibrosis (TIF) is the main pathologic feature of end-stage renal disease. Epithelial-mesenchymal transition (EMT) of proximal tubular cells (PTCs) is one of the most significant features of TIF. MicroRNAs play critical roles during EMT in TIF. However, whether miRNAs can be used as therapeutic targets in TIF therapy remains undetermined. We found that miR-30e, a member of the miR-30 family, is deregulated in TGF-β1-induced PTCs, TIF mice and human fibrotic kidney tissues. Moreover, transcription factors that induce EMT, such as snail, slug, and Zeb2, were direct targets of miR-30e. Using a cell-based miR-30e promoter luciferase reporter system, Schisandrin B (Sch B) was selected for the enhancement of miR-30e transcriptional activity. Our results indicate that Sch B can decrease the expression of snail, slug, and Zeb2, thereby attenuating the EMT of PTCs during TIF by upregulating miR-30e, both in vivo and in vitro. This study shows that miR-30e can serve as a therapeutic target in the treatment of patients with TIF and that Sch B may potentially be used in therapy against renal fibrosis
研究领域:
肾病学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Chunfan Huang,Xiaoqi Jing,Qianhu Wu,Kan Ding
Renal fibrosis is the final common result of a variety of progressive injuries leading to chronic renal failure. However, there are no effective clinical available drugs for the treatment. Notoginsenoside from Panax notoginseng could ameliorate renal fibrosis. We hypothesized that polysaccharide from this herb might have similar bioactivity. Here, we elucidated structure of a novel pectin-like polysaccharide designed SQD4S2 with a netty antenna backbone of glucogalacturonan substituted by glucoarabinan, glucurogalactan and galactose residues from this herb. Interestingly, SQD4S2 could reverse the morphological changes of human renal tubular HK-2 cells induced by TGF-β. Mechanism study suggested that this bioactivity might associate with N-cadherin (CDH2), Snail (SNAI1), Slug (SNAI2) depression and E-cadherin (CDH1) enhancement. In addition, SQD4S2 could impede critical fibrogenesis associated molecules such as α-SMA, fibronectin , vimentin , COL1A1, COL3A1, FN1 and ACTA2 expression induced by TGF-β in HK-2 cells. Current findings outline a novel leading polysaccharide for against renal fibrosis new drug development.
研究领域:
药理学
·
肾病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2020
·Vol. 24
·期 8
·4480-4493
·
IF 4.486
Linhao Xu,Yanli Bi,Yizhou Xu,Zhuocheng Zhang,Wenjie Xu,Sisi Zhang,Jian Chen
Small cell lung cancer (SCLC) is a severe malignant with high morbidity; however, few effective and secure therapeutic strategy is used in current clinical practice. Oridonin is a small molecule from the traditional Chinese herb Rabdosia rubescens. This study mainly aimed to investigate the role of oridonin on inhibiting the process of H1688, a kind of small cell lung cancer cells from human. Oridonin could suppress H1688 cell proliferation and induce their apoptosis in a high dosage treatment (20 μmol/L). Meanwhile, cell migration was suppressed by oridonin (5 and 10 μmol/L) that did not affect cell proliferation and apoptosis. The expression level of E-cadherin was significantly increased, and the expression of vimentin, snail and slug was reduced after administration of oridonin. These expression changes were associated with the suppressed integrin β1, phosphorylation of focal adhesion kinase (FAK) and ERK1/2. In addition, oridonin (5 and 10 mg/kg) inhibited tumour growth in a nude mouse model; however, HE staining revealed a certain degree of cytotoxicity in hepatic tissue after treatment oridonin (10 mg/kg). Furthermore, the concentration of alanine aminotransferase (ALP) was significantly increased and lactate dehydrogenase (LDH) was reduced after oridonin treatment (10 mg/kg). Immunohistochemical analysis further revealed that oridonin increased E-cadherin expression and reduced vimentin and phospho-FAK levels in vivo. These findings indicated that oridonin can inhibit the migration and epithelial-to-mesenchymal transition (EMT) of SCLC cells by suppressing the FAK-ERK1/2 signalling pathway. Thus, oridonin may be a new drug candidate to offer an effect of anti-SCLC with relative safety.
研究领域:
肿瘤学
·
药理学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2023
·
IF 5.1
Shuai Wang,Jian-Tao Shi,Xing-Rong Wang,Hong-Xia Mu,Xue-Ting Wang,Kai-Yan Xu,Qing-Shan Wang,Shi-Wu Chen
The PI3K/AKT/mTOR signaling pathway is one of the most common abnormal activation pathways in tumor cells, and has associated with multiple functions such as tumor cell growth, proliferation, migration, invasion, and tumor angiogenesis. Here, a series of 3-amino-1 H -indazole derivatives were synthesized, and their antiproliferative activities against HT-29, MCF-7, A-549, HepG2 and HGC-27 cells were evaluated. Among them, W24 exhibited the broad-spectrum antiproliferative activity against four cancer cells with IC 50 values of 0.43–3.88 μM. Mechanism studies revealed that W24 inhibited proliferation by affecting the DNA synthesis, induced G 2 /M cell cycle arrest and apoptosis by regulating Cyclin B1, BAD and Bcl-xL, meanwhile induced the change of intracellular ROS and mitochondrial membrane potential in HGC-27 cells. Moreover, W24 inhibited the migration and invasion of HGC-27 cells by decreasing EMT pathway related proteins and reducing the mRNA expression levels of Snail, Slug and HIF-1α. Furthermore, W24 displayed low tissue toxicity profile and good pharmacokinetic properties in vivo . Therefore, 3-amino-1 H -indazole derivatives might serve as a new scaffold for the development of PI3K/AKT/mTOR inhibitor and anti-gastric cancer reagent.
研究领域:
肿瘤学
·
药理学
·
化学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）