MICROBIOLOGICAL RESEARCH
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2024
·
IF 6.1
Yue-Ying Qin,Yuan Gong,Su-Yun Kong,Zhi-Yuan Wan,Jia-Qi Liu,Ke Xing,Sheng Qin
Plant-associated streptomycetes play important roles in plant growth and development. However, knowledge of volatile-mediated crosstalk between Streptomyces spp . and plants remains limited. In this study, we investigated the impact of volatiles from nine endophytic Streptomyces strains on the growth and development of plants. One versatile strain, Streptomyces setonii WY228, was found to significantly promote the growth of Arabidopsis thaliana and tomato seedlings, confer salt tolerance, and induce early flowering and increased fruit yield following volatile treatment. Analysis of plant growth-promoting traits revealed that S. setonii WY228 could produce indole-3-acetic acid, siderophores, ACC deaminase, fix nitrogen, and solubilize inorganic phosphate. These capabilities were further confirmed through genome sequencing and analysis. Volatilome analysis indicated that the volatile organic compounds emitted from ISP-2 medium predominantly comprised sesquiterpenes and 2-ethyl-5-methylpyrazine. Further investigations showed that 2-ethyl-5-methylpyrazine and sesquiterpenoid volatiles were the primary regulators promoting growth, as confirmed by experiments using the terpene synthesis inhibitor phosphomycin, pure compounds, and comparisons of volatile components. Transcriptome analysis, combined with mutant and inhibitor studies, demonstrated that WY228 volatiles promoted root growth by activating Arabidopsis auxin signaling and polar transport, and enhanced root hair development through ethylene signaling activation. Additionally, it was confirmed that volatiles can stimulate plant abscisic acid signaling and activate the MYB75 transcription factor, thereby promoting anthocyanin synthesis and enhancing plant salt stress tolerance. Our findings suggest that aerial signaling-mediated plant growth promotion and abiotic stress tolerance represent potentially overlooked mechanisms of Streptomyces -plant interactions. This study also provides an exciting strategy for the regulation of plant growth and the improvement of horticultural crop yields within sustainable agricultural practices.
研究领域:
植物学
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微生物学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Ming Zhao,Xiu-Shan Zhang,Liang-Bin Xiong,Kun Liu,Xiang-Fei Li,Yan Liu,Feng-Qing Wang
Indigoidine, as a kind of natural blue pigment, is widely used in textiles, food, and pharmaceuticals and is mainly synthesized from l-glutamine via a condensation reaction by indigoidine synthetases, most of which originates from Streptomyces species. However, due to the complex metabolic switches of Streptomyces, most of the researchers choose to overexpress indigoidine synthetases in the heterologous host to achieve high-level production of indigoidine. Considering the advantages of low-cost culture medium and simple culture conditions during the large-scale culture of Streptomyces, here, an updated regulation system derived from the Streptomyces self-sustaining system, constructed in our previous study, was established for the highly efficient production of indigoidine in Streptomyces lividans TK24. The updated system was constructed via promoter mining and σhrdB expression optimization, and this system was applied to precisely and continuously regulate the expression of indigoidine synthetase IndC derived from Streptomyces albus J1704. Finally, the engineered strain was cultured with cheap industrial glycerol as a supplementary carbon source, and 14.3 and 46.27 g/L indigoidine could be achieved in a flask and a 4 L fermentor, respectively, reaching the highest level of microbial synthesis of indigoidine. This study will lay a foundation for the industrial application of Streptomyces cell factories to produce indigoidine.
研究领域:
微生物学
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生物工程
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合成生物学
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DOI
PubMed
高影响因子
Methods in Enzymology
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2024
·
IF 1.682
Xingming Pan,Liao-Bin Dong
Magnesium ions (Mg 2+ ) are crucial in class II terpene cyclases that utilize substrates with diphosphate groups. Interestingly, these enzymes catalyze reactions without cleaving the diphosphate group, instead initiating the reaction through protonation . In our recent research, we discovered a novel class II sesquiterpene cyclase in Streptomyces showdoensis . Notably, we determined its crystal structure and identified Mg 2+ within its active site. This finding has shed light on the previously elusive question of Mg 2+ binding in class II terpene cyclases. In this chapter, we outline our methods for discovering this novel enzyme , including steps for its purification , crystallization , and kinetic analysis.
研究领域:
生物化学
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结构生物学
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DOI
高影响因子
NATURAL PRODUCT RESEARCH
·
2024
·
IF 1.9
Di Zhang,Yao Feng,Mingyi Chu,Yujiang Dai,Lu Jiang,Huifang Li
The infection of vibrio is an important cause of huge economic losses in aquaculture industry. At present, antibiotics are mainly used to prevent and reduce the infection of the vibrio, which has accelerated the emergence of multi-drug-resistant strains. New generation alternative anti-vibrio drugs were in urgent to solve this problem. In this study, six compounds (1–6) were isolated from the Streptomyces sp. ZZ741A, a marine-derived Streptomyces variant, including one new compound, 2-carbamoylphenyl isobutyrate (1), five known ones, nocardamine (2), dehydroxynocardamine (3), phenylacetic acid (4), thiophenol (5) and 2,3-dihydroxybenzoic acid (6). The anti-vibriosis assay showed that compounds 2 and 3 had specific inhibition activity against Vibrio vulnificus, Vibrio alginolyticus, and Vibrio parahaemolyticus with the MIC values ranging from 8 to 128 μg/mL. The molecular docking study of their possible mechanism of anti-vibriosis activity showed that the activity might come from the inhibition of Outer membrane protein U (OmpU).
研究领域:
微生物学
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水产养殖学
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药理学
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DOI
PubMed
高影响因子
TETRAHEDRON
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2018
·
IF 2.377
Mengxuan Chen,Weiyun Chai,Rongyao Zhu,Tengfei Song,Zhizhen Zhang,Xiao-Yuan Lian
Secondary metabolites from marine-associated actinomycetes are important source for the discovery of novel bioactive compounds. In this study, an actinomycete Streptomyces sp. ZZ446 was isolated from coastal soils and different media were used to culture this isolated marine actinomycete. It has been found that this actinomycete in the liquid medium of 2216 E with sea salt produced five new compounds of streptopyrazinones A−D ( 1 – 4 ) and N -acetyl- l -isoleucine- l -leucinamide ( 5 ) as well as six known diketopiperazines ( 6 – 11 ) and one alkaloid ( 12 ). Structures of the new compounds were determined by extensive NMR analyses, HRESIMS data, electronic circular dichroism (ECD) calculation, chemical degradation , Marfey's method, and X-ray diffraction analysis. This type of streptopyrazinones A−D ( 1 – 4 ) is rarely found in the natural resources. New compounds 1 – 5 showed activity in inhibiting the growth of Candida albicans and methicillin-resistant Staphylococcus aureus .
研究领域:
微生物学
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药物化学
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天然产物研究
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DOI
高影响因子
MOLECULES
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2022
·Vol. 27
·期 15
·5041
·
IF 4.927
Weiguo Wang,Minkang Feng,Xiaomeng Li,Feiyu Chen,Zhihao Zhang,Wenlong Yang,Chen Shao,Liming Tao,Yang Zhang
Actinomycetes play a vital role as one of the most important natural resources for both pharmaceutical and agricultural applications. The actinomycete strain SPRI-371, isolated from soil collected in Jiangsu province, China, was classified asStreptomyces aureusbased on its morphological, physiological, biochemical and molecular biological characteristics. Its bacterial activity metabolites were identified as aureonuclemycin (ANM), belonging to adenosine derivatives with the molecular formula C16H19N5O9for ANM A and C10H13N5O3for ANM B. Simultaneously, the industrial fermentation process of a mutatedS. aureusstrain SPRI-371 was optimized in a 20 m3fermentation tank, featuring a rotation speed of 170 rpm, a pressure of 0.05 MPa, an inoculum age of 36–40 h and a dissolved oxygen level maintained at 1–30% within 40–80 h and at >60% in the later period, resulting in an ANM yield of >3700 mg/L. In the industrial separation of fermentation broth, the sulfuric acid solution was selected to adjust pH and 4# resin was used for adsorption. Then, it was resolved with 20% ethanol solution and concentrated in a vacuum (60–65 °C), with excellent results. Antibacterial experiments showed that ANM was less active or inactive againstXanthomonas oryzaepv.oryzae,Xanthomonas citrisubsp.citriandXanthomonas oryzaepv.oryzicolaand most bacteria, yeast and fungi in vitro. However, in vivo experiments showed that ANM exhibited extremely significant protective and therapeutic activity against diseases caused byX. oryzaepv.oryzaeandX. oryzaepv.oryzicolain rice andX. citriin oranges and lemons. In field trials, ANM A 150 gai/ha + ANM B 75 gai/ha exhibited excellent therapeutic activity against rice bacterial leaf blight, citrus canker and rice bacterial leaf streak. Furthermore, as the dosage and production cost of ANM are lower than those of commercial drugs, it has good application prospects.Keywords:aureonuclemycin;Xanthomonas oryzaepv.oryzae;Xanthomonas citrisubsp.citri;Xanthomonas oryzaepv.oryzicola;antibacterial activity
研究领域:
药学
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农学
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微生物学
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DOI
PubMed
高影响因子
Genes
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2017
·Vol. 8
·期 12
·346
·
IF 3.6
Guoqiang Zhang,Dailin Yu,Bu Sang,Juntao Feng,Lirong Han,Xing Zhang
Streptomyces kanasensisZX01 produces some antibiotics and a glycoprotein with antiviral activity. To further evaluate its biosynthetic potential, here we sequenced the 7,026,279 bp draft genome ofS. kanasensisZX01 and analyzed all identifiable secondary gene clusters for controlling natural products. More than 60 putative clusters were found inS. kanasensisZX01, the majority of these biosynthetic loci are novel. In addition, the regulators for secondary metabolism inS. kanasensisZX01 were abundant. The global regulatornsdAnot only controls biosynthesis of some antibiotics, but also enhances production of glycoprotein GP-1 with antiviral activity. This study importantly reveals the powerful interplay between genomic analysis and studies of traditional natural product purification/production increasing.Keywords:genome mining;secondary metabolism;nsdA;regulator;glycoprotein GP-1
研究领域:
微生物学
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基因组学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF ANTIBIOTICS
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2016
·Vol. 70
·期 2
·216-218
·
IF 2.173
Gao Mei-yue,Qi Huan,Li Jian-song,Zhang Hui,Zhang Ji,Wang Ji-dong,Xiang Wen-sheng
Accessions GenBank/EMBL/DDBJ AF389344 KX118440
研究领域:
微生物学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR MICROBIOLOGY
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2016
·Vol. 103
·期 4
·713-728
·
IF 3.761
Tan-Jun Wang,Yi-Ming Shan,Han Li,Wei-Wang Dou,Xin-Hang Jiang,Xu-Ming Mao,Shui-Ping Liu,Wen-Jun Guan,Yong-Quan Li
Summary Antibiotic-producing microorganisms have evolved several self-resistance mechanisms to prevent auto-toxicity. Overexpression of specific transporters to improve the efflux of toxic antibiotics has been found one of the most important and intrinsic resistance strategies used by many Streptomyces strains. In this work, two ATP-binding cassette (ABC) transporter-encoding genes located in the natamycin biosynthetic gene cluster, scnA and scnB , were identified as the primary exporter genes for natamycin efflux in Streptomyces chattanoogensis L10. Two other transporters located outside the cluster, a major facilitator superfamily transporter Mfs1 and an ABC transporter NepI/II were found to play a complementary role in natamycin efflux. ScnA/ScnB and Mfs1 also participate in exporting the immediate precursor of natamycin, 4,5-de-epoxynatamycin, which is more toxic to S. chattanoogensis L10 than natamycin. As the major complementary exporter for natamycin efflux, Mfs1 is up-regulated in response to intracellular accumulation of natamycin and 4,5-de-epoxynatamycin, suggesting a key role in the stress response for self-resistance. This article discusses a novel antibiotic-related efflux and response system in Streptomyces , as well as a self-resistance mechanism in antibiotic-producing strains.
研究领域:
微生物学
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药理学
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遗传学
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DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2018
·
IF 2.533
Ming Zhao,Shui-Ling Wang,Xin-Yi Tao,Guo-Ling Zhao,Yu-Hong Ren,Feng-Qing Wang,Dong-Zhi Wei
Due to the lack of powerful gene regulation elements, the engineering development of Streptomyces is often limited. Here, we disclosed that the heterologous σ 70 -dependent promoters, which have been reported as inefficient tools for gene expression in Streptomyces , could be efficiently recognized by Streptomyces housekeeping factor σ hrdB . Therefore, an effective strategy was developed to engineer these promoters for robust gene expression in Streptomyces by fusing them with optimized 5′-untranslation regions (5′-UTRs). As a proof of concept, the widely used P tac in E. coli was engineered by fusing its core promoter region with the 5′-UTR R15 from a relatively powerful Streptomyces promoter P kasO * R15 and resulted in P tac* , the activity of which was 8.1-fold that of P tac and 1.7-fold that of P kasO * R15 in S. lividans TK24. Next, the 5′-UTR R15 was optimized by randomizing the ribosome binding site (RBS). Based on the base biases of those RBSs with higher activity, eight artificial RBSs were rationally designed, and the optimal resulting promoter P tac * RBS3 showed about 2.1, 3.6, and 17.6 times the activity of P tac *, P kasO * R15 , and P tac , respectively, demonstrating that the heterologous P tac was converted into a type of robust Streptomyces promoters. This study thus greatly expands promoter diversity for the engineering of Streptomyces .
研究领域:
合成生物学
·
分子遗传学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NATURAL PRODUCTS
·
2019
·
IF 4.257
Di Zhang,Wenwen Yi,Hengju Ge,Zhizhen Zhang,Bin Wu
The new streptoglutarimides A–J (1–10) and the known streptovitacin A (11) were isolated from a marine-derived actinomycete, Streptomyces sp. ZZ741. Structures of the isolated compounds were elucidated based on their HRESIMS data, extensive NMR spectroscopic analyses, ECD calculations, Mosher′s method, and a single-crystal X-ray diffraction experiment. Streptoglutarimide H (8) and streptovitacin A (11) showed potent antiproliferative activity against human glioma U87MG and U251 cells with IC50 values of 1.5–3.8 μM for 8 and 0.05–0.22 μM for 11. All isolated compounds exhibited antimicrobial activity with MIC values of 9–11 μg/mL against methicillin-resistant Staphylococcus aureus, 8–12 μg/mL against Escherichia coli, and 8–20 μg/mL against Candida albicans.
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DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Xuechen Si,Zuoyun Yuan,Huilin Li,Yunping Zhu,Yawen Zhou,Jia Liu,Zhichao Wu
Granaticins are natural pigments derived from microorganisms with promising bioactivity. However, their practical applications have been restricted due to inherent instability. To improve the stability of granaticins from the novel strain Streptomyces vilmorinianum YP1, microcapsules were prepared using gum Arabic (GA) by a freeze-drying method. The optimal parameters for microencapsulation were determined using response surface methodology. Under the optimal conditions (GA 9.2% ( v /v), a wall/−core ratio 4.8 ( w /w), encapsulating temperature 29 °C), the maximum encapsulation efficiency achieved was 93.64%. The microcapsules were irregular single crystals with an average particle size of 206.37 ± 2.51 nm. Stability testing indicated improved stability of the microencapsulated granaticins. Notably, granaticnic B retention increased by 17.0% and 6.6% after exposure to sunlight and storage at 4 °C, respectively. These finding suggest that GA as a well material significantly enhances the stability of granaticins from S. vilmorinianum YP1, facilitating their potential applications.
研究领域:
生物技术
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药学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
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