JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Ran Ao,Jinyan Liu,Xiaohui Ren,Yuelong Liu,Yan Jia
In this paper, corrosion inhibition performance of Helianthus tuberosus L extracts(HTLE) and Poria cocos extracts(PCSE) alone and compounded with Na 2 SiO 3 in 0.01 mol L −1 NaOH solution on AA2024-T3 aluminum alloy was investigated by weight loss, electrochemical impedance spectroscopy, potential polarization, atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS), quantum chemical calculations and molecular dynamics simulations. Infrared spectroscopy was used to study the main active components in both extracts. The results revealed that when the two extracts were utilized separately, the corrosion inhibition impact was insignificant. However, when compounded with Na 2 SiO 3 , the corrosion inhibition efficiency was greatly enhanced. Fixing the concentration of Na 2 SiO 3 at 610 mg L −1 , the inhibition effectiveness rose with the increase of the concentration of the extracts, and when the concentration of HTLE, PCSE were 800 mg L −1 and 1000 mg L −1 , respectively, the inhibition efficiencies could reached approximated 98 %. The two compounds were anodic-dominant hybrid corrosion inhibitors. AFM and XPS analysis revealed that the two compounds could successfully adsorb on the aluminum alloy surface, protecting the metal from corrosion. Theoretical simulations were conducted by selecting the three major components from each of the two extracts. Quantitative chemistry calculations indicated the accepted and donated electron groups of the major components. Molecular dynamics simulations show that the primary components compounded with Na 2 SiO 3 have a higher negative adsorption energy than their action alone.
研究领域:
材料科学
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电化学
·
量子化学
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DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2022
·
IF 6.371
H.H. Zhang,Y.W. Liu,H. Bian,Y. Zhang,Z.N. Yang,Z. Zhang,Y. Chen
Hydrophobic silane/reduced graphene oxide (rGO) nanocomposites were successfully constructed on an AA2024-T3 aluminum alloy surface using a facile co-electrodeposition process. The composition and morphology were characterized by ATR-FTIR, XRD , Raman spectroscopy , EDX , XPS and SEM. The results revealed that the implanted GO nanosheets were partially reduced and successfully covalently bonded with silanol groups to fabricate a more compact and denser coating. Electrochemical measurements demonstrated that the electrodeposited silane/rGO nanocomposite markedly decreased the corrosion rate of the AA2024-T3 alloy in 3.5 wt% NaCl solution. The protection efficiency was maintained over 96% after 120 h of exposure, presenting excellent chemical stability. Meanwhile, a sand paper abrasion test was performed to evaluate the mechanical robustness and the contact angle was reduced by only 4.5° after 150 abrasion cycles. The excellent anti-corrosion property and mechanical robustness, along with the feasible electrodeposition procedure, show its great potential for industrial applications.
研究领域:
材料科学
·
腐蚀与防护
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DOI
高影响因子
ACS Omega
·
2020
·
IF 2.87
Pakapa Thiangpak,Aphichart Rodchanarowan
The shape-memory epoxy (SME) mixed with 10 wt % polycaprolactone (PCL) microspheres containing 5% cerium(III) nitrate (Ce(NO3)3) (PCL5Ce) was coated on an aluminum plate 2024-T3 to investigate the self-healing property. The coating was scratched and heated at 80 °C for 30 min to activate the self-healing mechanism and compare with a nonscratched coating. Surface morphology was investigated by scanning electron microscopy. The scratch was completely healed by the PCL5Ce via a thermally assisted self-healing process. Based on electrochemical impedance spectroscopy, the postheated scratched coating had shown impedance values close to the nonscratched coating, which indicated that corrosion resistivity was restored. Ce(NO3)3 content at the scratched area was analyzed by focused ion beam–scanning electron microscopy. The scratch width was healed and filled with Ce(NO3)3. Therefore, PCL5Ce is capable of being used as an enhancing additive for the self-healing performance in SME coating.
研究领域:
材料科学
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腐蚀与防护
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表面工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FISH BIOLOGY
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2016
·Vol. 88
·期 5
·1693-1707
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IF 1.246
W. T. Zhang,K. Liu,J. S. Xiang,L. Y. Zhang,W. J. Liu,Z. D. Dong,Y. Z. Li,H. L. Li,S. L. Chen,N. Wang
To elucidate the effect of thyroid hormone receptor α A ( thraa ) on metamorphosis, the full length cDNA of half-smooth tongue sole Cynoglossus semilaevis was cloned. The relative gene transcript level of thraa at different development stages was quantified using real-time PCR. Transcription of thraa increased and declined rapidly during metamorphosis. Hyperthyroidism was induced in juveniles and larvae with exposure to T3 and T4, and hypothyroidism with thiourea (TU), 2-mercapto-1-methylimidazole (MMI). thraa mRNA was higher in fish treated for 6 days with MMI than in untreated controls, although inhibited larvae did not complete metamorphosis. The addition of exogenous T4 reversed this effect in the MMI-treated group, but not in the TU-treated group. In situ hybridization revealed progressive tail end of body growth and change during developmental stages, with corresponding changes in thraa expression. This process may be induced by thyroid hormones with thraa as a major mediator. The morphological changes of tip of the tail may be associated with the development of lateral swimming.
研究领域:
分子生物学
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内分泌学
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发育生物学
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水产养殖
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DOI
PubMed
高影响因子
Food Science & Nutrition
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2022
·Vol. 10
·期 6
·1841-1853
·
IF 3.553
Jun Yang,Shulin Hu,Anqi Liao,Yetian Weng,Shuli Liang,Ying Lin
Current chemical analysis approaches for contaminants have failed to reveal their biotoxicity. Moreover, conventional bioassays are time consuming and exhibit poor repeatability. In this study, we performed the acute toxicity detection of various contaminants (chromium (Cr), cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg), tin (Sn), nickel (Ni), and bisphenol A (BPA)) with four bioluminescent bacteria ( Vibrio qinghaiensis Q67, V. fischeri , Photobacterium phosphoreum T3, and P. phosphoreum 502) using a rapid, flexible, and low-cost bioassay. We found that the temperature affected the bacterial luminescence, and freeze-dried cells exhibited sensitive toxic responses to contaminants. Indeed, the optimized protectants containing 12% (w/v) trehalose, 4% sucrose, and 2% sorbitol displayed better luminescence and toxic sensitivity. Furthermore, freeze-dried powders of these strains were prepared and subjected to acute toxicity detection. The results showed that all contaminants exhibited acute toxicity toward Q67, but the other strains did not show obvious response to nickel and tin. The relative half-maximal effective concentration (EC 50 ) values of BPA, Cr, Cd, Pb, As, Hg, Ni, and Sn to Q67 were 0.674, 1.313, 11.137, 5.921, 4.674, 0.911, 5.941, and 54.077 mg/L, respectively. In addition, the EC 50 values of contaminants toward different strains were suggested to be statistically significant. Freeze-dried Q67 exhibited toxic responses to more contaminants than the other bioluminescent strains; therefore, Q67 was selected to be more suitable than the other strains for single and mixture toxicity detection tests. Compared with other strains, Q67 was more appropriate for the rapid screening of the mixture toxicity of contaminants in samples as a nonspecific screening sensor before the use of standard analysis approaches.
研究领域:
环境科学
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毒理学
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生物技术
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化学分析
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Jia Gao,Jing Ren,Hanjie Ye,Wenhui Chu,Xuankai Ding,Lingzhi Ding,Yongqian Fu
Angiogenesis is pivotal for osteogenesis during bone regeneration. A hydrogel that promotes both angiogenesis and osteogenesis is essential in bone tissue engineering. However, creating scaffolds with the ideal balance of biodegradability, osteogenic, and angiogenic properties poses a challenge. Thymosin beta 10 (TMSB10), known for its dual role in angiogenesis and osteogenesis differentiation, faces limitations due to protein activity preservation. To tackle this issue, ZIF-8 was engineered as a carrier for TMSB10 (TMSB10@ZIF-8), and subsequently integrated into the self-assembled sericin hydrogel. The efficacy of the composite hydrogel in bone repair was assessed using a rat cranial defect model. Characterization of the nanocomposites confirmed the successful synthesis of TMSB10@ZIF-8, with a TMSB10 encapsulation efficiency of 88.21 %. The sustained release of TMSB10 from TMSB10@ZIF-8 has significantly enhanced tube formation in human umbilical vein endothelial cells (HUVECs) in vitro and promoted angiogenesis in the chicken chorioallantoic membrane (CAM) model in vivo . It has markedly improved the osteogenic differentiation ability of MC 3 T3-E1 cells in vitro . 8 weeks post-implantation, the TMSB10@ZIF-8/ Sericin hydrogel group exhibited significant bone healing (86.77 ± 8.91 %), outperforming controls. Thus, the TMSB10@ZIF-8/Sericin hydrogel, leveraging ZIF-8 for TMSB10 delivery, emerges as a promising bone regeneration scaffold with substantial clinical application potential.
研究领域:
生物医学工程
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组织工程
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材料科学
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再生医学
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DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2015
·Vol. 31
·期 12
·2068-2080
·
IF 3.197
Farooq Ahmad,Xiaoyi Liu,Ying Zhou,Hongzhou Yao,Fangfang Zhao,Zhaoxing Ling,Chao Xu
Fascinating super paramagnetic uniqueness of iron oxide particles at nano-scale level make them extremely useful in the state of the art therapies, equipments, and techniques. Cobalt ferrite (CoFe 2 O 4 ) magnetic nanoparticles (MNPs) are extensively used in nano-based medicine and electronics, results in extensive discharge and accumulation into the environment. However, very limited information is available for their endocrine disrupting potential in aquatic organisms. In this study, the thyroid endocrine disrupting ability of CoFe 2 O 4 NPs in Zebrafish larvae for 168-h post fertilization ( hpf ) was evaluated. The results showed the elevated amounts of T4 and T3 hormones by malformation of hypothalamus pituitary axis in zebrafish larvae. These elevated levels of whole body THs leads to delayed hatching, head and eye malformation, arrested development, and alterations in metabolism. The influence of THs disruption on ROS production and change in activities of catalase (CAT), mu -glutathione s-transferase ( mu -GST), and acid phosphatase (AP) were also studied. The production of significantly higher amounts of in vivo generation of ROS leads to membrane damage and oxidative stress. Presences of NPs and NPs agglomerates/aggregates were also the contributing factors in mechanical damaging the membranes and physiological structure of thyroid axis. The increased activities of CAT, mu -GST, and AP confirmed the increased oxidative stress, possible DNA, and metabolic alterations, respectively. The excessive production of in vivo ROS leads to severe apoptosis in head, eye, and heart region confirming that malformation leads to malfunctioning of hypothalamus pituitary axis. ROS-induced oxidative DNA damage by formation of 8-OHdG DNA adducts elaborates the genotoxicity potential of CoFe 2 O 4 NPs. This study will help us to better understand the risk and assessment of endocrine disrupting potential of nanoparticles. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 2068–2080, 2016.
研究领域:
环境毒理学
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纳米技术
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水生生物学
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内分泌学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Zhihui Liu,Yingying Shangguan,Penglin Zhu,Yousef Sultan,Yiyi Feng,Xiaoyu Li,Junguo Ma
Glyphosate (GLY) induces developmental toxicity in fish, but research on the toxicity mechanism is limited. In this study, zebrafish embryos were exposed for 120 hpf to 0.7, 7, and 35 mg L -1 GLY. The results show that GLY treatment induced developmental toxicity in the fish, including premature hatching, reduced heartbeats, pericardial and yolk sac oedema, swim bladder deficiency, and shortened body length, which was possibly due to a significantly decreased triiodothyronine (T3)/thyroxine (T4) ratio and the abnormal expression patterns of hypothalamic-pituitary-thyroid (HPT) ( crh , tshβ , tr α , tr β , and t tr ) and growth hormone/insulin-like growth factor (GH/IGF) axis-related genes ( gh , ghrα , ghrβ , igf1 , igf1rα , and igf1rβ ) in larvae exposed to GLY. In addition, GLY exposure altered the levels of SOD and CAT, increased ROS, promoted malondialdehyde (MDA) content, and significantly altered the levels of endoplasmic reticulum (ER) stress signalling pathway factors ( perk , eif2α , gadd34 , atf4 , ire1α , xbp1 , atf6 , hspa5 , and chop ), suggesting that GLY treatment induced oxidative injury and ER stress in the larvae. Further research showed that treatment with a higher concentration of GLY upregulated the levels of iNOS, IL-1β, and TNF-α while inhibiting the expression of IL-10 and TGF-β, suggesting that GLY causes an inflammatory reaction in the larvae. In addition, we also found that apoptosis was induced in the larvae, which was determined by acridine orange staining and abnormal expression of p53 , caspase-3 , -8 , and -9 . Taken together, our results demonstrate that GLY exposure altered the T3/T4 ratio, disturbed the expression patterns of HPT and GH/IGF axis-related genes, and induced oxidative and ER stress, inflammatory reactions, and apoptosis in the zebrafish larvae. This investigation contributes to improved understanding of the developmental toxicity mechanism of GLY in fish.
研究领域:
毒理学
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发育生物学
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环境科学
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鱼类生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Jianmei Lv,Qiping Wu,Sanwang Li,Hanxi Yi,Feifan Xie
Bacterial infection is a leading cause of neonatal death . Ceftazidime , commonly used for neonatal infections , is often used off-label. Blood sampling limits pharmacokinetic (PK) studies in neonatal patients. The dried blood spots (DBS) are a potential matrix for microsampling. Herein, we describe an ultra-performance liquid chromatography with a photodiode array (UPLC-PDA) to determine ceftazidime in DBS from neonatal patients in support of pharmacokinetic studies. The Capitainer® device-based DBS samples containing 10 µL blood were extracted in 70% methanol/water ( v/v ) with acetaminophen as the internal standard (IS). The extraction process was carried out at 20 °C using a block bath shaker at 1000 rpm for 30 min. The extracted ceftazidime was subsequently eluted through an Acquity UPLC HSS T3 column (2.1 × 50 mm, 1.8 µm). Elution was achieved using a water (containing 0.1% trifluoroacetic acid)/acetonitrile linear gradient at a flow rate of 0.5 mL/min, and the analytical time was 3.2 min. The PDA detection wavelength was set at 259 nm. The method underwent thorough validation following the recommendation of the European Bioanalysis Forum (EBF) and the bioanalytical guideline established by the European Medicines Agency (EMA). No interfering peaks were detected at the retention times of ceftazidime and IS. The ceftazidime exhibited a quantification range spanning from 0.5 to 200 µg/mL, and the assay demonstrated good accuracy (intra/inter-assay ranging from 90.1% to 104.8%) and precision (intra/inter-assay coefficient of variations ranging from 4.8% to 11.7%). The method's applicability was demonstrated by analyzing clinical DBS samples collected from neonatal patients.
研究领域:
药理学
·
分析化学
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新生儿学
·
临床研究
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2019
·
IF 4.527
Shengxian Liang,Shaojun Liang,Nuoya Yin,Francesco Faiola
The liver is one of the major targets of hormones, including thyroid hormones (THs), and many industrial chemicals, such as endocrine-disrupting chemicals. Those compounds may permeate the placenta barrier and pose a risk for embryonic development . Therefore, it is necessary to assess the toxic effects of those kind of industrial chemicals during liver development. In this study, to mimic liver specification in vitro, we differentiated human embryonic stem cells (ESCs) into functional hepatocyte-like cells. We performed this differentiation process in presence of two THs, triiodothyronine (T3) and thyroxine (T4), with the purpose of identifying biomarkers for toxicity screening. TH exposure (3, 30 and 300 nM) yielded to hepatocytes with impaired glycogen storage ability and abnormal lipid droplets’ accumulation. Global gene expression analysis by RNA-seq identified a number of genes responsible for hepatic differentiation and function which were affected by 30 nM T3 and T4. Those differentially expressed genes were used to assess the potential developmental liver toxicity of two famous environmental pollutants, 2, 2, 4, 4-tetrabromodiphenyl ether (BDE-47) and decabromodiphenyl ether (BDE-209), at 10 nM to 1 μM treatments. Our findings demonstrate that BDE-47 and BDE-209, dysregulated pathways such as “chemical carcinogenesis”, “steroid hormone biosynthesis” and “drug metabolism-cytochrome P450”. Moreover, we were able to identify a set of 17 biomarkers, very useful to predict the potential developmental hepatotoxicity of industrial chemicals.
研究领域:
毒理学
·
干细胞生物学
·
发育生物学
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环境科学
·
药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）