SCIENCE OF THE TOTAL ENVIRONMENT
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2020
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IF 6.551
Ping Xiang,Kun Wang,Jue Bi,Mengying Li,Rui-Wen He,Daolei Cui,Lena Q. Ma
Indoor dust often contains organic contaminants, which adversely impacts human health. In this study, the organic contaminants in the indoor dust from commercial offices and residential houses in Nanjing, China were extracted and their effects on human breast cancer cells (MCF-7) were investigated. Both dust extracts promoted proliferation of MCF-7 cells at ≤24 μg/100 μL, with cell viability being decreased with increasing dust concentrations. Based on LC 50 , house dust was less toxic than office dust. At 8 μg/100 μL, both extracts caused more MCF-7 cells into active cycling (G2/M + S) and increased intracellular Ca 2+ influx, with house dust inducing stronger effects than office dust. Further, the expression of estrogen-responsive genes for TFF1 and EGR3 was enhanced by 3-9 and 4-9 folds, while the expression of cell cycle regulatory genes for cyclin D was enhanced by 2-5 folds. The results suggested that organic dust extract influenced cell viability, altered cell cycle, increased intracellular Ca 2+ levels, and activated cell cycle regulatory and estrogen-responsive gene expressions, with house dust showing lower cytotoxicity but higher estrogenic potential on MCF-7 cells. The results indicate the importance of reducing organic contaminants in indoor dust to mitigate their adverse impacts on human health.
研究领域:
毒理学
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环境科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 27
·70109-70120
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IF 5.8
Deng Dengxian,Wang Junxia,Xu Sijie,Sun Yueying,Shi Guangyu,Wang Huili,Wang Xuedong
This study investigated the physiological and biochemical impacts of organophosphate flame retardants (OPFRs) on wheat ( Triticum aestivum L.) germination and growth performance in the presence and absence of copper. The study evaluated seed germination, growth, OPFRs concentrations, chlorophyll fluorescence index (F v /F m and F v /F 0 ), and antioxidant enzyme activity. It also calculated the root accumulation of OPFRs and their root-stem translocation. At the germination stage, at a concentration of 20 μg·L −1 OPFR exposure, wheat germination vigor, root, and shoot lengths were significantly decreased compared to the control. However, the addition of a high concentration of copper (60 mg·L −1 ) decreased by 80%, 82%, and 87% in the seed germination vitality index and root and shoot elongation, respectively, compared to 20 μg·L −1 of OPFR treatment. At the seedling stage, a concentration of 50 μg·L −1 of OPFRs significantly decreased by 42% and 5.4% in wheat growth weight and the photochemical efficiency of photosystem II (Fv/Fm) compared to the control. However, the addition of a low concentration of copper (15 mg·L −1 ) slightly enhanced the growth weight compared to the other two co-exposure treatments, but the results were not significant ( p > 0.05). After 7 days of exposure, the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) (indicates lipid peroxidation) content in wheat roots significantly increased compared to the control and was higher than in leaves. MDA contents in wheat roots and shoots were decreased by 18% and 6.5% when OPFRs were combined with low Cu treatment compared with single OPFRs treatment, but SOD activity was slightly improved. These results suggest that the co-exposure of copper and OPFRs enhances reactive oxygen species (ROS) production and oxidative stress tolerance. Seven OPFRs were detected in wheat roots and stems, with root concentration factors (RCFs) and translocation factors (TFs) ranging from 67 to 337 and 0.05 to 0.33, respectively, for the seven OPFRs in a single OPFR treatment. The addition of copper significantly increased OPFR accumulation in the root and aerial parts. In general, the addition of a low concentration of copper promoted wheat seedling elongation and biomass and did not significantly inhibit the germination process. OPFRs could mitigate the toxicity of low-concentration copper on wheat but had a weak detoxification effect on high-concentration copper. These results indicated that the combined toxicity of OPFRs and Cu had antagonistic effects on the early development and growth of wheat.
研究领域:
环境科学
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植物生理学
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农业
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毒理学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Kun Chen,Guixin Wang,Xuedong Wang,Huili Wang
It is of great importance to develop the highly efficient fluorescence strategy for rapid/sensitive detection of metam-sodium (MES) in evaluating its residual safety, especially in fresh vegetables. Herein, we prepared an organic fluorophore (thiochrome, TC) and glutathione-capped copper nanoclusters (GSH-CuNCs), and their combination (TC/GSH-CuNCs) was sucessfully employed as a ratiometric fluoroprobe by means of the blue-red dual emission. The fluorescence intensities (FIs) of TC decreased upon the addition of GSH-CuNCs via the fluorescence resonance energy transfer (FRET) process. When fortified at the constant levels of GSH-CuNCs and TC, MES substantially reduced the FIs of GSH-CuNCs, while this was not the case in the FIs of TC except for the prominent red-shift of ∼30 nm. Compared to the previous fluoroprobes, the TC/GSH-CuNCs based fluoroprobe supplied wider linear range of 0.2–500 μM, lower detection limit (60 nM), and satisfactory fortification recoveries (80–107%) for MES in the cucumber samples. Based on the fluorescence quenching phenomenon, a smartphone application was used to output RGB values of the captured images for the colored solution. The smartphone-based ratiometric sensor could be utilized for the visual fluorescent quantitation of MES by virtue of the R/B values in cucumbers, which gave linear range (1–200 μM) and LOD (0.3 μM). By means of blue-red dual-emission fluorescence, the smartphone-based fluoroprobe provides a cost-effective, portable and reliable avenue for the on-site, rapid and sensitive assay of MES's residues in complex vegetable samples.
研究领域:
食品安全检测
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分析化学
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生物传感
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）