APPLIED CATALYSIS B-ENVIRONMENTAL
·
2026
·
IF 21.1
Penghao Li,Mengmeng Xu,Jinzeng Guo,Diandian Lv,Mingwang Shao,Kui Yin
Strain engineering has emerged as an effective strategy to modulate the electronic structure and adsorption energetics of oxygen evolution reaction (OER) catalysts. Among various approaches, phase-transition-induced strain offers distinct advantages due to its intrinsic origin, controllable magnitude, and structural stability, providing a self-adaptive route for dynamic catalytic regulation. Here, a lattice-matching strategy is employed to construct a tetragonal-phase ZrO 2 and tetragonal-phase RuO 2 (Tet-ZrO 2 /RuO 2 ) heterojunction catalyst. During synthesis, the phase transition of intermediate ZrO 2 (Int-ZrO 2 ) induces partial compressive strain on RuO 2 , leading to shortened Ru-O and Ru-Ru bond distances. The resulting Tet-ZrO 2 /RuO 2 catalyst exhibits outstanding activity in 0.5 M H 2 SO 4 , requiring only 193 mV overpotential to achieve 10 mA cm −2 and demonstrates remarkable stability, maintaining performance for 1000 h at 10 mA cm −2 with negligible degradation. Combining in situ attenuated total reflectance surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) with density functional theory calculation, it is revealed that the Tet-ZrO 2 /RuO 2 heterojunction enables the coexistence of both the adsorbate evolution mechanism (AEM) and the oxide path mechanism (OPM), jointly promoting O 2 generation. This work elucidates the regulatory role of lattice strain in tuning OER mechanisms through novel support design, providing a novel paradigm for developing highly active and stable electrocatalysts.
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高影响因子
Communications Biology
·
2020
·Vol. 3
·期 1
·1-10
·
IF 4.165
Liu Yuan,Jia Yuqian,Yang Kangni,Li Ruichao,Xiao Xia,Wang Zhiqiang
Recent emergence of high-level tigecycline resistance mediated by Tet(X3/X4) in Enterobacteriaceae undoubtably constitutes a serious threat for public health worldwide. Antibiotic adjuvant strategy makes antibiotic more effective against these resistant pathogens through interfering intrinsic resistance mechanisms or enhancing antibiotic actions. Herein, we screened a collection of drugs to identify compounds that are able to restore tigecycline activity against resistant pathogens. Encouragingly, we discovered that anti-HIV agent azidothymidine dramatically potentiates tigecycline activity against clinically resistant bacteria. Meanwhile, addition of azidothymidine prevents the evolution of tigecycline resistance in E. coli and the naturally occurring horizontal transfer of tet (X4). Evidence demonstrated that azidothymidine specifically inhibits DNA synthesis and suppresses resistance enzyme activity. Moreover, in in vivo infection models by Tet(X4)-expression E. coli , the combination of azidothymidine and tigecycline achieved remarkable treatment benefits including increased survival and decreased bacterial burden. These findings provide an effective regimen to treat infections caused by tigecycline-resistant Escherichia coli .
研究领域:
微生物学
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感染病学
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药理学
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PubMed
高影响因子
ACS Applied Energy Materials
·
2024
·
IF 5.4
Jian Lv,Zhuyu Wang,Yihuan Zhou,Yiwen Wang,Danni Shen,Qinggong Jia
Cathodes containing Ni, Co, and Mn frequently undergo side reactions with the electrolyte during the long cycles of the battery in traditional electrolytes under high voltage. In this work, an efficient composite cathode–electrolyte interface (CEI) film is constructed in situ with two additives; that is, 2-[(trimethylsilyl)ethynyl]thiophene (TET) is oxidized and polymerized in advance to form a film attached to the cathode surface, while oxidation products of bis(pinacolato)diboron (BDB) as a high ion conductor are doped into the TET polymer film. The combination of the oxidation products of the two additives not only effectively isolates solvent molecules and hydrofluoric acid erosion, preventing the occurrence of side reactions, but also does not affect the transfer of lithium ions. The crystal structure stability of LiNi0.8Mn0.1Co0.1O2 (NCM811) could be effectively regulated by the composite CEI film through in situ X-ray diffraction characterization during the charge–discharge process. The dynamic performance of the battery has also been improved by comparing the discrepancy of pseudocapacitance across the battery with different electrolytes. Furthermore, the solid–electrolyte interface film formed by TET and BDB could provide effective protection for lithium metal from electrolyte corrosion and inhibit the formation of lithium dendrites. Therefore, the battery capacity retention rate could reach 91.12% after 300 cycles. In addition, the battery with a high-load cathode (2.52 mAh cm–2) has a capacity retention rate of up to 90.19% after 50 cycles, which may make the future application of pouch batteries more feasible.
研究领域:
材料科学
·
电化学
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高影响因子
ANALYST
·
2025
·
IF 3.3
Jiahui Lai,Danni Wang,Xiuqin Sheng,Chuan-Fan Ding,Yinghua Yan
In large-scale glycoproteomics studies, reducing the experimental duration and enhancing digestion efficiency are crucial. In this work, a magnetic nanomaterial featuring a surface rich in hydrophilic groups and uniformly distributed trypsin (Fe3O4@DOPA/PEI@Au@DNA TET-trypsin) was developed for efficient digestion of proteins and specific enrichment of glycopeptides. The material surface exhibits a regular distribution of trypsin coupled with abundant hydrophilic groups. Thus, the material can demonstrate excellent performance in experimental studies related to glycoproteomics. Fe3O4@DOPA/PEI@Au@DNA TET-trypsin showed an excellent limit of detection (0.001 amol·μL−1) and high selectivity for glycopeptides (BSA : HRP = 5000 : 1) in a short reaction time, and it showed good performance in the processing of practical samples. Gene Ontology (GO) analysis suggests that complement activation, extracellular space, antioxidant activity and complement binding may be associated with tuberculosis. These analytical results provide new insights for determining and clarifying the pathogenesis and pathology related to tuberculosis.
研究领域:
蛋白质组学
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生物化学
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医学
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PubMed
高影响因子
ACS Chemical Biology
·
2017
·
IF 4.995
Jun Xiong,Xiaona Liu,Qing-Yun Cheng,Shan Xiao,Lai-Xin Xia,Bi-Feng Yuan,Yu-Qi Feng
Toxic heavy metals have been considered to be harmful environmental contaminations. The molecular mechanisms of heavy-metals-induced cytotoxicity and carcinogenicity are still not well elucidated. Previous reports showed exposures to toxic heavy metals can cause a change of DNA cytosine methylation (5-methylcytosine, 5-mC). However, it is still not clear whether heavy metals have effects on the recently identified new epigenetic marks in both DNA and RNA, i.e., 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-foC), and 5-carboxylcytosine (5-caC). Here, we established a chemical labeling strategy in combination with liquid chromatography–electrospray ionization–mass spectrometry (LC-ESI-MS/MS) analysis for highly sensitive detection of eight modified cytidines in DNA and RNA. The developed method allowed simultaneous detection of all eight modified cytidines with improved detection sensitivities of 128–443-fold. Using this method, we demonstrated that the levels of 5-hmC, 5-foC, and 5-caC significantly decreased in both the DNA and RNA of mouse embryonic stem (ES) cells while exposed to arsenic (As), cadmium (Cd), chromium (Cr), and antimony (Sb). In addition, we found that treatments by heavy metals induced a decrease of the activities of 10–11 translocation (Tet) proteins. Furthermore, we revealed that a content change of metabolites occurring in the tricarboxylic acid cycle may be responsible for the decline of the derivatives of 5-mC. Our study shed light on the epigenetic effects of heavy metals, especially for the induced decline of the derivatives of 5-mC in both DNA and RNA.
研究领域:
毒理学
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表观遗传学
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干细胞生物学
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分析化学
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高影响因子
JOURNAL OF ANTIBIOTICS
·
2021
·Vol. 74
·期 5
·330-336
·
IF 2.649
Lan Sugui,Li Zhirong,Su Aiqiu,Peng Yanhong,Liao Yanke,Liu Xuemei,Tan Qiang
The purpose of this study was focused on the mechanisms of the cross-resistance to tetracycline (TET), piperacillin Sodium (PIP), and gentamicin (GEN) in Staphylococcus aureus (SA) mediated by Rhizoma Coptidis extracts (RCE). The selected strains were exposed continuously to RCE at the sublethal concentrations for 12 days, respectively. The susceptibility change of the drug-exposed strains was determined by analysis of the minimum inhibitory concentration. The 16S rDNA sequencing method was used to identify the RCE-exposed strain. Then the expression of resistant genes in the selected isolates was analyzed by transcriptome sequencing. The results indicated that RCE could trigger the preferential cross-resistance to TET, PIP, and GEN in SA. The correlative resistant genes to the three kinds of antibiotics were upregulated in the RCE-exposed strain, and the mRNA levels of the resistant genes determined by RT-qPCR were consistent with those from the transcriptome analysis. It was suggested from these results that the antibacterial Traditional Chinese Medicines might be a significant factor of causing the bacterial antibiotic-resistance.
研究领域:
微生物学
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药理学
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分子生物学
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PubMed
高影响因子
Journal of Materials Chemistry B
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2022
·Vol. 10
·期 25
·4810-4822
·
IF 7.571
Boyou Zhang,Fei Sun,Yi Lu,Zhihao Wang,Zhiming Shen,Lei Yuan,Qiang Wu,Cong Wu,Hongcan Shi
Long segment trachea defects are repaired by tracheal substitution, while decellularized technology has been effectively employed to prepare tissue engineering trachea (TET). However, its clinical application is restricted by the long preparation cycle, while poor vascularization is associated with the transplantation failure. In the present study, we used sodium lauryl ether sulfate (SLES) to develop a novel rapid decellularized tracheal preparation method, then constructed a TET with revascularization functions. Summarily, we decellularized rabbit trachea using various SLES concentrations. Results from histological analysis, immunohistochemical and DAPI staining, as well as DNA quantitative assay, revealed that 1–0.1% (v/v) SLES treatment not only entirely removed cellular components to reduce its immunogenicity, but also retained the tracheal matrix's gross structure. SEM images, safranine O-fast green staining, total collagen content assay and collagen II immunofluorescence revealed that low SLES concentrations preserved the bioactive components of the decellularized tracheal matrix. Next, we performed cytobiocompatible and cytotoxin assays to verify biocompatibility of the decellularized tracheal matrix, and is confirmed by the omentum transplantation of rats. Results from omentum transplantation revealed that the decellularized tracheal matrix had low immunogenicity and excellent biocompatibility. Its revascularization capacity was confirmed by histologic appearance and CD31 immunofluorescence. Based on these findings, we selected 0.1% (v/v) as the optimal SLES concentration for preparing a decellularized tracheal matrix. Next, we seeded allogeneic bone marrow stem cells (BMSC) onto the matrix to construct TET patches. In vivo tracheal defect reconstruction confirmed the biocompatibility and revascularization capacity of this novel TET, and the formation of a vascular network around the patch promoted submucosa and mucosa regeneration without significant stenosis, 4 weeks post-surgery. In conclusion, we used SLES to successfully develop a novel decellularized approach for the preparation of TET, which has low immunogenic and inflammatory responses, as well as excellent biocompatibility, and revascularization ability in vivo without additional exogenous cytokines.
研究领域:
组织工程
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再生医学
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生物医学工程
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移植学
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高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Langsha Yi,Wei Zhang,Zeyou Chen,Hui Li,Yahai Lu,Shu Tao,Dongqiang Zhu
Phototransformation is a key process affecting the fate of many antibiotics in the environment, but little is known about whether their photoproducts exert selective pressure on bacteria by inducing antibiotic resistance genes (ARGs). Here, we examined the expression of tetracycline resistance gene tet(M) of a fluorescent Escherichia coli whole-cell bioreporter influenced by the phototransformation of tetracycline. The presence of suspended smectite clay (montmorillonite or hectorite, 1.75 g/L) or dissolved humic substance (Pahokee Peat humic acid or Pahokee peat fulvic acid, 10 mg C/L) in aqueous solutions markedly facilitated the transformation of tetracycline (initially at 400 μg/L) with half-life shortened by 1.4–2.6 times. Despite the similar phototransformation ratios (80–90%) of the total loaded tetracycline after 60 min irradiation, the decreased ratios of cell fluorescence intensity (which was proportional to the expression amount of ARG tet(M)) were much higher with the two clays (94 and 93%) than with the two humic substances (44 and 69%) when compared to the respective dark controls. As illustrated by mass spectroscopic and chemical analyses, tetracycline was proposed to be mainly transformed to amide (ineffective in inducing ARGs) with the presence of clays by reaction with self-photosensitized singlet oxygen (1O2), while the humic substances might catalyze the production of another two demethylated and/or deaminated compounds (still effective in inducing ARGs) in addition to the amide compound via reaction with triplet excited state dissolved organic matter (3DOM*). As clay minerals and humic substances are important soil constituents and ubiquitously present in surface environments, the observed clay and humic-dependent photooxidation pathways of tetracycline and the differing selective pressures of the associated products highlight the need for monitoring the transformation compounds of antibiotics and provide critical insight into the development of antibiotic treatment protocols.
研究领域:
环境科学
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微生物学
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化学
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高影响因子
Frontiers in Cell and Developmental Biology
·
2022
·
IF 6.081
Deng Changping,Hu Fabiao,Zhao Zhangting,Zhou Yiwen,Liu Yuping,Zhang Tong,Li Shihui,Zheng Wenyun,Zhang Wenliang,Wang Tianwen,Ma Xingyuan
Quantitative analysis and regulating gene expression in cancer cells is an innovative method to study key genes in tumors, which conduces to analyze the biological function of the specific gene. In this study, we found the expression levels of Survivin protein (BIRC5) and P-glycoprotein (MDR1) in MCF-7/doxorubicin (DOX) cells (drug-resistant cells) were significantly higher than MCF-7 cells (wild-type cells). In order to explore the specific functions of BIRC5 gene in multi-drug resistance (MDR), a CRISPR/Cas9-mediated knocking-in tetracycline (Tet)-off regulatory system cell line was established, which enabled us to regulate the expression levels of Survivin quantitatively (clone 8 named MCF-7/Survivin was selected for further studies). Subsequently, the determination results of doxycycline-induced DOX efflux in MCF-7/Survivin cells implied that Survivin expression level was opposite to DOX accumulation in the cells. For example, when Survivin expression was down-regulated, DOX accumulation inside the MCF-7/Survivin cells was up-regulated, inducing strong apoptosis of cells (reversal index 118.07) by weakening the release of intracellular drug from MCF-7/Survivin cells. Also, down-regulation of Survivin resulted in reduced phosphorylation of PI3K, Akt, and mTOR in MCF-7/Survivin cells and significantly decreased P-gp expression. Previous studies had shown that PI3K/Akt/mTOR could regulate P-gp expression. Therefore, we speculated that Survivin might affect the expression of P-gp through PI3K/Akt/mTOR pathway. In summary, this quantitative method is not only valuable for studying the gene itself, but also can better analyze the biological phenomena related to it.
研究领域:
癌症生物学
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分子生物学
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基因工程
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药理学
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PubMed
高影响因子
Animals
·
2025
·Vol. 15
·期 24
·3564
·
IF 2.7
Kaiwei Yang,Xi Wu,Bingcun Ma,Jianguo Cheng,Zengting Li,Yin Wang,Zexiao Yang,Xueping Yao,Yan Luo
Simple SummaryEscherichia coli(E. coli) is an opportunistic pathogen and an important reservoir of antibiotic resistance genes. Thetet(X4) gene mediates tigecycline resistance by encoding a flavin-dependent monooxygenase. However, the mechanism by which this gene influences bacterial antibiotic resistance through regulating the expression of other genes is complex and not yet fully understood. In this study,E. coli tet(X4) deletion and complemented strains were constructed to explore the effects of this gene on bacterial growth and antibiotic resistance. We measured the minimum inhibitory concentrations (MICs) of 24 antibiotics and the degradation capability for tetracycline/tigecycline in the wild type, deletion, and complemented strains, and conducted a 4-week starvation stress experiment (with/without subinhibitory concentrations of tigecycline) to analyze growth curves, survival rates, and changes in MICs. Transcriptome sequencing identified 531 differentially expressed genes, which are associated with ABC transporter activity, drug metabolism, and two-component systems. These findings provide reliable insights into the mechanism by which thetet(X4) gene affectsE. coliantibiotic resistance and offer a reference for the prevention and control of tigecycline-resistantE. coliinfections.Escherichia coli(E. coli) is a ubiquitous opportunistic pathogen in nature and serves as an important reservoir for antibiotic resistance genes. Thetet(X4) gene is a key determinant mediating tigecycline resistance. Although its core resistance mechanism, encoding a flavin-dependent monooxygenase, has been elucidated, the broader impact of thetet(X4) gene on the secondary regulatory networks ofE. coliremains not fully understood. In recent years, multiple studies have indicated that thetet(X4) gene participates in pathways contributing to resistance to other antibiotics by regulating the expression of various genes. In this study,E. coli tet(X4) gene deletion and complementation strains were constructed to investigate the mechanisms by which thetet(X4) gene influences the growth characteristics and antibiotic resistance ofE. coli. The minimum inhibitory concentrations (MICs) of 24 different antibiotics, as well as the degradation capacities of tetracycline and tigecycline, were determined for the wild-type, deletion, and complementation strains. In addition, a four-week starvation stress experiment was performed under both the presence and absence of sub-inhibitory concentrations of tigecycline, during which the bacterial growth curves, survival rates, and MIC variations were analyzed. Transcriptomic sequencing of the wild-type, deletion, and complementation strains identified 531 differentially expressed genes associated with ABC transporter activity, drug metabolism, and bacterial two-component systems. These findings provide reliable evidence for elucidating the mechanism by which thetet(X4) gene affectsE. coliresistance, offering valuable insights into the prevention and control of tigecycline-resistantE. coliinfections.
研究领域:
微生物学
·
抗生素耐药
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分子遗传学
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转录组学
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传染病学
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高影响因子
Journal of Environmental Sciences
·
2022
·
IF 6.796
Jianbin Zhao,Xinyan Li,Yanbin Xu,Yuxin Li,Li Zheng,Tiangang Luan
Direct discharge of aquaculture wastewater may have toxic effects, due to the presence of heavy metals, antibiotics, and even resistant pathogens , but little attention has been given. Here, tanks simulating a wild ecosystem were built to study the effects of long-term exposure to duck wastewater containing oxytetracycline (OTC) and/or arsenic (As) on the growth, physiological function, and gut microbiota evolution of Xenopus tropicalis . The results showed that duck wastewater had no apparent impact on X. tropicalis , but the impact increased significantly ( P < 0.05) with exposure to OTC and/or As, especially the impact on body weight and growth rate. Biochemical indicators revealed varying degrees of oxidative stress damage, hepatotoxicity (inflammation, necrosis, and sinusoids), and collagen fibrosis of X. tropicalis in all treated groups after 72 days of exposure, which indirectly inhibited X. tropicalis growth. Moreover, 16S rDNA amplicon sequencing results showed that the gut microbiota structure and metabolic function were perturbed after chronic exposure, which might be the leading cause of growth inhibition. Interestingly, the abundance of intestinal resistance genes (RGs) increased with exposure time owing to the combined direct and indirect effects of stress factors in duck wastewater. Moreover, once the RGs were expressed, the resistance persisted for at least 24 days, especially that conferred by tet A. These results provide evidence of the toxic effects of DW containing OTC (0.1–4.0 mg/L) and/or As (0.3–3.5 µg/L) on amphibians and indicate that it is vital to limit the usage of heavy metals and antibiotics on farms to control the biotoxicity of wastewater.
研究领域:
环境科学
·
毒理学
·
微生物学
·
水生生物学
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Xiuli Chen,Yanchu Ke,Ying Zhu,Mingbang Xu,Chao Chen,Shuguang Xie
Tetracycline (TC) is an antibiotic that is recently found as an emerging pollutant with low biodegradability . Biodegradation shows great potential for TC dissipation. In this study, two TC-degrading microbial consortia (named SL and SI) were respectively enriched from activated sludge and soil. Bacterial diversity decreased in these finally enriched consortia compared with the original microbiota. Moreover, most ARGs quantified during the acclimation process became less abundant in the finally enriched microbial consortia. Microbial compositions of the two consortia as revealed by 16 S rRNA sequencing were similar to some extent, and the dominant genera Pseudomonas , Sphingobacterium, and Achromobacter were identified as the potential TC degraders. In addition, consortia SL and SI were capable of biodegrading TC (initial 50 mg/L) by 82.92% and 86.83% within 7 days, respectively. They could retain high degradation capabilities under a wide pH range (4−10) and at moderate/high temperatures (25–40 °C). Peptone with concentrations of 4–10 g/L could serve as a desirable primary growth substrate for consortia to remove TC through co-metabolism. A total of 16 possible intermediates including a novel biodegradation product TP245 were detected during TC degradation . Peroxidase genes, tet X-like genes and the enriched genes related to aromatic compound degradation as revealed by metagenomic sequencing were likely responsible for TC biodegradation.
研究领域:
环境微生物学
·
生物修复
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分子生态学
·
宏基因组学
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高影响因子
危险品化学品经营许可证（带存储）