APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2022
·
IF 5.005
Hao Ren,Haixia Wang,Yang Wang,Yiyang Chen,Zhenmei Lu
Tetrahydrofuran (THF) has been recognized as a water contaminant because of its human carcinogenicity, extensive use, and widespread distribution. Previously reported multicomponent monooxygenases (MOs) involved in THF degradation were highly conserved, and all of them were from Gram-positive bacteria. In this study, a novel THF-degrading gene cluster (dmpKLMNOP) encoding THF hydroxylase was identified on the chromosome of a newly isolated Gram-negative THF-degrading bacterium, Cupriavidus metallidurans ZM02, and functionally characterized. Transcriptome sequencing and RT-qPCR demonstrated that the expression of dmpKLMNOP was upregulated during the growth of strain ZM02 on THF or phenol. The deletion of oxygenase alpha or beta subunit or the reductase component disrupted the degradation of THF but did not affect the utilization of its hydroxylated product 2-hydroxytetrahydrofuran. Cupriavidus pinatubonensis JMP134 heterologously expressing dmpKLMNOP from strain ZM02 could grow on THF, indicating that the THF hydroxylase DmpZM02KLMNOP is responsible for the initial degradation of THF. Furthermore, the THF and phenol oxidation activities of crude enzyme extracts were detected, and the highest THF and phenol catalytic activities were 1.38 ± 0.24 μmol min−1 mg−1 and 1.77 ± 0.37 μmol min−1 mg−1, respectively, with the addition of NADPH and Fe2+. The characterization of THF hydroxylase associated with THF degradation enriches our understanding of THF-degrading gene diversity and provides a novel potential enzyme for the bioremediation of THF-containing pollutants.IMPORTANCE Multicomponent MOs catalyzing the initial hydroxylation of THF are vital rate-limiting enzymes in the THF degradation pathway. Previous studies of THF degradation gene clusters have focused on Gram-positive bacteria, and the molecular mechanism of THF degradation in Gram-negative bacteria has rarely been reported. In this study, a novel THF hydroxylase encoded by dmpKLMNOP in strain ZM02 was identified to be involved in both THF and phenol degradation. Our findings provide new insights into the THF-degrading gene cluster and enzymes in Gram-negative bacteria.
研究领域:
环境微生物学
·
生物技术
·
酶学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
IONICS
·
2024
·
IF 2.4
Chen Zhenyu,Du Zhenyu,Wang Tianxiang,Chen Lei
Lithium-sulfur (Li/S) batteries using tetrahydrofuran (THF) as an electrolyte solvent have high discharge capacity. In this study, we investigate the effect of THF as an electrolyte solvent on the performance of Li/S batteries. Charge/discharge tests show that Li/S batteries employing THF as an electrolyte solvent have higher discharge specific capacity than those employing 1,3-dioxolane (DOL) and 1,2-dimethyl ether (DME) at low current densities. The reaction rate of S with Li metal and the shuttle effect of polysulfide ions is lower in THF than in DME. Compared to DME and DOL, THF has a higher dielectric constant, and its solvation ability is weaker than that of DME, which is more conducive to the deposition of lithium ions. Electrochemical impedance spectroscopy shows that the charge transfer resistance of symmetric Li metal batteries in THF is lower than that in DOL/DME. THF forms a gel layer that restrains the shuttle effect during battery discharge and increases battery impedance. The addition of DOL to THF can effectively inhibit the formation of a gel layer and improve the coulombic efficiency of the system. Finally, ternary mixed solvents are prepared, and batteries with the new electrolyte solvent reveal better discharge capacity and higher coulombic efficiency than those with the original solvents. Taking the results together, THF appears to be a promising solvent for Li/S batteries.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
PROPELLANTS EXPLOSIVES PYROTECHNICS
·
2025
·
IF 2
Mengjing An,Yunjun Luo
Energetic thermoplastic elastomers (ETPEs) play important roles in the study of energetic adhesives. However, the contradiction between good mechanical properties and high energy constrains its further application. Using isocyanates with different structures, a poly(BAMO-THF) (PBT) energetic thermoplastic elastomer with a disordered hard-phase structure was synthesized. The special structure imparts a high mobility and a better network relaxation of molecular chains, which results in higher toughness, lower glass transition temperature, and greater self-healing ability than commonly used ETPEs. Additionally, the large but disordered hard domains enable the prominent crystallization potential of 3,3-bis(azidomethyl) oxetane (BAMO) segments to be released. Therefore, it results in better mechanical properties at a lower content of hard segments by strain-induced crystallization, achieving a balance between mechanical and energetic properties. At 20% content of hard segments, the enthalpy of formation is 1.54 kJ/g, which makes PBT elastomers have a broader application prospect in the field of explosives and propellants. Therefore, this work is of great significance in guiding the design of multifunctional materials with high energy and high mechanical properties.
研究领域:
材料科学
·
含能材料
·
高分子化学
·
热塑性弹性体
·
推进剂与炸药
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2014
·
IF 3.766
Fangxia Zhu,Qinglai Yang,Yuan Zhuang,Yuanqing Zhang,Zhifeng Shao,Bing Gong,Yu-Mei Shen
Developing smart nanocarriers for drug delivery system is advantageous for many kinds of successful biomedicinal therapy. In this study, we designed an amphiphilic block copolymers containing pH-responsive tetrahydropyran (THP) and tetrahydrofuran (THF) linkage. Their structures were confirmed by 1 H NMR and gel permeation chromatography (GPC). The release rate of encapsulated drugs depends upon the pH value and pH sensitive linkage in the backbone of copolymers . For PLA–THP–PEG micelles the cumulative release amount of doxorubicin (DOX) was 62% at pH 5.0, which is about four times higher than that at pH 7.4. Under the same conditions the release rate for PLA–THF–PEG micelles is a little faster than that of the PLA–THP–PEG micelles. Cellular uptake study demonstrates that DOX-loaded micelles can easily enter the cells and produce the desired pharmacological action and minimizing the side effect of free DOX. These findings indicate that THP and THF linked diblock copolymer micelles is a promising candidate for drug carrier.
研究领域:
生物医学
·
药剂学
·
高分子化学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Binglin Chen,Guizhuan Xu,Zhangbin Zheng,Dongxiang Wang,Caihong Zou,Chun Chang
An efficient and green conversion process of producing 5-ethoxymethylfurfural (EMF) from corn stover was developed. The solid zeolite USY provided the good catalytic performance in ethanol medium with tetrahydrofuran (THF) as co-solvent, which can promote the formation of EMF, reducing the formation of ethyl levulinate and 5-hydroxymethylfurfural. The reaction conditions were optimized via response surface methodology leading to a maximum EMF yield of 21.8% in ethanol/THF (volume ratio 1:1) under the conditions of 168 °C, 1 wt% USY loading and 2.9 h. Meanwhile, USY can be reused in five consecutive reaction runs with high activity, upholding the same initial activity and structural integrity. A plausible reaction pathway for the conversion of corn stover to EMF in ethanol/THF medium was further proposed. This study showed that the improved catalytic system presents an efficient method for direct production of EMF from lignocellulosic biomass.
研究领域:
生物质化学
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
SOLID STATE SCIENCES
·
2023
·
IF 3.5
Xiaoqin Feng,Huijun Li,Weina Wu,Yuan Wang,Zhouqing Xu,Jia Fu
Herein, a multifunctional mechanochromic Metal-Organic Framework ( HPU-27 ) was designed for detecting water in THF and adsorbing Cr(VI) from contaminated water. The probe displays a reasonable quenching response for H 2 O over a concentration range of 0–80% with a detection limit of 0.039%, accompanied by an obvious fluorescent color transformation from yellow to colorless. The detection mechanism of HPU-27 toward H 2 O was proved as the alternation of the physical states between aggregation and dispersion in THF and H 2 O. Meanwhile, the HPU-27 material showed a good ability to adsorb Cr(VI) from contaminated water. The maximum adsorption capacity is 294.94 mg/g. In addition, the adsorption mechanism was discussed in detail. Thus, we believe that our strategy is promising extensively for the development of multifunctional MOF materials.
研究领域:
材料科学
·
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
FirePhysChem
·
2026
·
IF 5.1
Xin Ruan,Yousheng Qiu,Wenwen Deng,Ligang Zhang,Jiaoyang Liu,Zeyu Wang,Hongxia Zhang,Chongwei An,Baoyun Ye
During the storage, transportation and loading processes of composite solid propellants, micro-cracks and defects will be generated, which will shorten their service life. By developing composite solid propellants with self-repairing functions, it is expected to solve these problems. Therefore, this study proposes a novel energetic self-healing binder based on dynamic disulfide bond exchange reactions and hydrogen-bond reorganization. BAMO/THF is copolymerized with GAP matrix as the soft segment; isophorone diisocyanate (IPDI) and 2,2′-diaminodiphenyl disulfide (2-AFD) are selected as the hard segments to synthesize a series of self-repairing adhesives (PGPU). After 96 hours of high and low temperature impact, the mass change rate of PGPU samples is less than 0.01%, the tensile strength change rate is less than 6%, and the fracture elongation rate change rate is less than 4%. It has excellent mechanical stability under high and low temperature environmental impact. Among them, the toughness of PGPU-3 reaches 38.93 MJ·m -3 , and the self-repair efficiency reaches 84.47% after 16 hours of fracture at 75 °C. Based on this, self-repairing composite solid propellants are prepared, and the self-repairing efficiency of the tensile strength can reach 84.28%, and the burning rates at 1 MPa, 3 MPa, and 5 MPa pressures are 10.07 mm/s, 12.19 mm/s, and 13.82 mm/s, respectively, with excellent combustion performance. This study provides new theoretical basis and feasibility schemes for designing high-energy-density self-repairing adhesives.
✓ 已验证
产品关联
DOI
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2023
·Vol. 18
·期 3
·e2901
·
IF 1.8
Yu Jia,Haoran Zhao,Yihang Chen,Xuanyu Liang,Hongge Tao,Guizhuan Xu,Chun Chang
The reaction characteristics and mechanism of carbohydrates is important in exploring and developing suitable technology for biofuels and biochemical production from lignocellulosic biomass. In this study, experiments combined with density functional theory (DFT) calculation and molecular dynamics (MD) simulation were used to investigate the glucose alcoholysis reaction mechanism catalyzed by extremely low concentrated sulfuric acid. The effects of temperature and tetrahydrofuran (THF) on the distribution of the product in the alcoholysis process of high-concentration glucose were investigated. DFT calculation results showed that the addition of THF in ethanol can decrease the energy barrier of glucose degradation. Meanwhile, the MD simulation indicated that the catalyst tends to localize more around the glucose molecular, which was beneficial to the glucose degradation in ethanol. Combining the experimental and theoretical results, plausible glucose alcoholysis reaction pathways were proposed in this work. It was found that when temperature was lower than 190°C, the three main pathways were glucose conversion into ethyl glucoside (EDGP); glucose conversion into 1,6-anhydro-β-d-glucopyranose (LG), then further to levoglucosone (LGO), furans, or 1,4:3,6-dianhydro-α-d-glucopyranose (DGP); and glucose conversion to fructose. However, at higher temperatures (190 and 220°C), the main reaction pathways were, glucose conversion into LG, LGO, and ethyl levulinate (EL); glucose conversion into fructose, 5-hydroxymethylfurfural (HMF), 5-ethoxymethylfurfural (EMF), and EL; and glucose conversion into humins. DFT, MD simulation, and experimental results showed that the addition of THF is beneficial for the EDGP formation from high concentration glucose catalyzed by Brønsted acid.
研究领域:
生物化学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Central South University
·
2020
·Vol. 26
·期 11
·2974-2986
·
IF 1.249
Xu Si-quan,Pan Dong-hui,Xiao Guo-min
With the aim of achieving a high 5-hydroxymethylfurfural (HMF) yield from glucose with H-ZSM-5 catalyst at low cost, three inexpensive biphasic reaction systems, H 2 O—tetrahydrofuran (THF), H 2 O—2-methyltetrahydrofuran (MeTHF) and H 2 O’2-butanol, were discovered and proved to be particularly effective in promoting the formation of HMF from glucose over H-ZSM-5 zeolite. In order to determine the optimal process conditions, the effects of various experimental variables, such as reaction temperature, reaction time, catalyst dosage, volume of organic solvent, as well as inorganic salt type on glucose conversion to HMF in three systems were investigated in detail. It was found that under optimal reaction conditions, H 2 O—THF, H 2 O—2-butanol and H 2 O—MeTHF allowed the glucose dehydration process to achieve HMF yields of up to 61%, 59%, and 50%, respectively. Moreover, in the three biphasic systems, the H-ZSM-5 catalyst was also demonstrated to maintain excellent stability. Thus, the catalytic approach proposed in this paper can be believed to have potential prospects for industrially efficient and low-cost production of HMF.
研究领域:
化学工程
·
生物质转化
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED ENERGY
·
2024
·
IF 10.1
Yaning Kong,Honglin Yu,Mengqi Liu,Guodong Zhang,Fei Wang
Hydrate was considered to be the next generation hydrogen storage material , and hydrate-based hydrogen storage (HBHS) would play an important role in future hydrogen economy, but sluggish hydrate formation kinetics presents huge technological challenge for its application. Herein, a nano promoter of -SO 3 − @PSNS was prepared by soap-free emulsion polymerization , and a dual kinetic promoter system (L-methionine/-SO 3 − @PSNS) was applied to enhance the formation kinetics of mixed H 2 /DIOX hydrate. At a moderate condition (12 MPa and 274.15 K), hydration efficiency under a driving force of 2.1 MPa is significantly enhanced by the synergy of the dual kinetic promoters, hydrate induction time reduces from 443.2 ± 209.73 min to 24.7 ± 17.16 min, while hydrate growth rate increases by 43.75%, and hydrogen storage capacity is up to 0.473 ± 0.041 wt% that increases by 62.8%. At a more moderate condition (10 MPa and 274.15 K) with an extremely low driving force of 0.1 MPa, enhanced hydrate formation was obtained in a composite promotion system of the dual kinetic promoters and dual thermodynamic promoters (THF/DIOX), which also performs exceptionally in term of recycling. The presence of a small amount (0.35 mol%) of THF reduces the induction time by 93.5%, and hydrate growth rate increases by 130.18%, while satisfactory hydrogen storage capacity (0.346 ± 0.017 g/g) is still remained in mixed H 2 /DIOX/THF hydrate, the structure of which was confirmed by Raman spectrum . This work presents an effect approach on enhancing the formation kinetics of H 2 -contained hydrate under low driving force, and this finding provides important insight for HBHS.
研究领域:
物理化学
·
能源材料
·
气体储存
·
化学工程
·
纳米材料科学
·
分子模拟
·
可持续能源
✓ 已验证
DOI
高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
·
2014
·Vol. 51
·期 4
·1094-1099
·
IF 0.873
Guangfan Han,Jiawei Du,Lizhuang Chen,Lijun Zhao
A series of 2-amino-7-methoxy-4-aryl-4 H -chromene-3-carbonitrile compounds 2 were obtained by condensation of 3-methoxyphenol with β-dicyanostyrenes 1 in absolute ethanol containing piperidine. The intermediate enamines 3 were prepared by compounds 2 with 5-substituted-1,3-cyclohexanedione using p -toluenesuflonic acid (TsOH) as catalyst. The title compounds 11-amino-3-methoxy-8-substituted-12-aryl-8,9-dihydro-7 H -chromeno[2,3- b ]quinolin-10(12 H )-one 4 were synthesized by cyclization of the intermediate enamines 3 in THF with K 2 CO 3 /Cu 2 Cl 2 as catalyst. The structures of all compounds were characterized by elemental analysis, IR, MS, and 1 H NMR spectra. The crystal structure of compound 4i was determined by single-crystal X-ray diffraction analysis.
研究领域:
有机化学
·
药物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2023
·
IF 5.3
Xu Zhang,Haowen Cui,Qinfang Li,Haian Xia
It is of great significance to develop an affordable and environmentally friendly method for producing 5-hydroxymethylfurfural (HMF) and furfural from inexpensive and abundant agricultural waste. Herein, FePO4 combined with organic acids such as mandelic acid were used as catalysts in a one-pot catalytic conversion of untreated wheat straw into HMF and furfural in a two-phase system of THF/H2O saturated by NaCl. H+, Fe(OH)2+, and Fe(OH)2+ originated from partially dissolved FePO4 in hot water together with organic acids provided Brønsted and Lewis acid sites for the cascade conversion of wheat straw. Whereas organic acid could regulate the concentrations of Lewis and Brønsted acid sites to promote the dehydration process of fructose and hinder the production of by-products and humins, boosting the yield of HMF and furfural. The response surface methodology (RSM) was used to optimize the reaction conditions. The highest yield of HMF was 62.9% at 200 °C and 120 min, which is higher than the reported yield of HMF using lignocellulose as raw material in the current literature. Furfural has also achieved a higher yield (93.9%) at 180 °C and 90 min. This study demonstrates the rationality of the deployment of lignocellulose to produce furan compounds by organic acids and FePO4, providing the possibility for efficient production of HMF and furfural from recalcitrant agricultural waste.
研究领域:
化学工程
·
绿色化学
·
生物炼制
·
可持续化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）