Analytical Methods
·
2016
·Vol. 8
·期 20
·4119-4126
·
IF 1.915
Lin Lin,Dongmin Shi,Quanfa Li,Guangfeng Wang,Xiaojun Zhang
Determination of nucleotide kinase activity is valuable due to its importance in regulating nucleic acid metabolism. In this work, a novel and simple colorimetric method for discriminative detection of T4 polynucleotide kinase (PNK) through a MnO2 nanosheet-3,3′,5,5′-tetramethylbenzidine (TMB) colorimetric system is developed. In aqueous solution at pH 7.4, the MnO2 nanosheet, a mimic enzyme, rapidly converts colourless TMB to a blue oxidized form. In the presence of T4PNK, a hairpin DNA could be phosphorylated and further cleaved by λ exonuclease (λ exo) and the ssDNA fragments would adsorb on the surface of the MnO2 nanosheet, resulting in the inhibition of the catalytic effect of the MnO2 nanosheet to the oxidation of TMB and weakening of the blue color of oxidized TMB with the absorption peak centred at 652 nm. The presence of T4PNK can in this way easily be detected with the naked eye, based on the obvious colour change. The experimental results revealed that the advanced strategy was sensitive for detecting T4PNK in the concentration range from 0.005 to 10 U mL−1 with a detection limit of 0.005 U mL−1. In addition, the relative standard deviation was 3.92% in 3 repetitive assays of 10 U mL−1 T4PNK, indicating that the reproducibility of this strategy was acceptable. By comparison with other relevant fluorogenic assays, the sensitivity of the developed chromogenic assays was satisfactory. The present approach opens the possibility for the design of simple and sensitive colorimetric assays for polynucleotide kinase using inexpensive and available TMB as a universal chromogenic compound. Therefore, besides desirable sensitivity and selectivity, due to the colorimetric system, the developed method for T4PNK detection also showed cost-effective, reliable and simplified operations and strategy, which has considerable potential for biological process research.
研究领域:
生物化学
·
分子生物学
·
分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-10
·
IF 5.705
Ding Yongqi,Zhao Junfeng,Li Bo,Zhao Xu,Wang Chao,Guo Minghui,Lin Yuqing
The authors report that cobalt oxyhydroxide (CoOOH) nanoflakes possess intrinsic oxidizing ability to directly oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue colored product (oxTMB) even in the absence of H 2 O 2 and oxygen. In the presence of ascorbic acid (AA), less of the blue product is formed because AA reduces oxTMB. These findings constitute a new scheme for colorimetric detection of AA. Absorbance, best measured at 652 nm, linearly drops in the 10 nM to 1 μM AA concentration range, and the limit of detection is 5 nM (at an S/N ratio of 3). The reaction is complete within <5 s and highly selective. A strip test has been designed for fast and on-spot visual detection of AA. The method was applied to the direct estimation of AA in the microdialysate of brain, and also in soft drink samples. The strip test is considered to be a promising tool for the rapid screening of AA in brain and commercial samples.
Schematic of the CoOOH-TMB colorimetric system that exhibits a high selectivity for ascorbic acid (AA). A strip test has been designed for fast and on-spot visual detection of AA.
研究领域:
分析化学
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生物医学工程
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食品科学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Pengjuan Ni,Yujing Sun,Haichao Dai,Jingting Hu,Shu Jiang,Yilin Wang,Zhuang Li
Glutathione (GSH) plays an important role in the biological system and serves many cellular functions. Since all of the biothiols possess similar functional groups, it is still challenging to selectively detect GSH over cysteine (Cys) and homocysteine (Hcy). In this work, a novel and simple colorimetric method for discriminative detection of glutathione (GSH) over Cys and Hcy is developed. The proposed method is based on the fact that Ag [I] ion could oxidize 3,3′,5,5′,-tetramethylbenzidine (TMB) to the oxidized TMB to induce a blue color and an absorption peak centered at 652 nm. However, the introduction of GSH could cause the reduction of oxidized TMB and it could also combine with Ag + , both of which result in a blue color fading and a decrease of the absorbance at 652 nm. Based on this finding, we propose a method to qualitatively and quantitatively detect GSH by naked eyes and UV–vis spectroscopy, respectively. The proposed method shows a low detection limit of 0.1 µM by naked eyes and 0.05 µM with the help of UV–vis spectroscopy. In addition, this method has great potential in discriminatively detecting GSH over other amino acid and biothiols. More importantly, this method is simple and fast without the preparation of nanomaterials and has also been successfully applied to the detection of GSH in biological fluids.
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Pengjuan Ni,Yujing Sun,Haichao Dai,Shu Jiang,Wangdong Lu,Yilin Wang,Zhen Li,Zhuang Li
A novel colorimetric method for the detection of acetylcholinesterase (AChE) and its inhibitor by taking utilization of Ag [I] ion–3,3′,5,5′-tetramethylbenzidine (TMB) detection system has been proposed. Ag [I] ion could oxidize TMB to induce a blue color and an absorption peak centered at 652 nm. In the presence of AChE, acetylthiocholine (ACh) was hydrolyzed to thiocholine. Ag [I] ion could interact with thiocholine and thiocholine can reduce the oxidized TMB (oxTMB) to TMB, both of which suppressed the oxidation capacity of Ag [I] ion, resulting in a blue color fading and a decrease of the absorbance at 652 nm. The developed assay is highly sensitive with a low detection limit of 4.3 μU/mL. Moreover, the method was also applied in AChE inhibitor sensing. The obtained assay is fairly simple, inexpensive and sensitive without the utilization of nanomaterials, which may be not only used for the sensing of other hydrolytic enzyme activities with properly selected substrates, but also used for the screening of potential inhibitor drugs.
研究领域:
生物化学
·
医学检测
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Mohammad Imran,Muhammad Ashfaq Ahmad,Akhtar Hayat,Essam R.I. Mahmoud,Cong Kong,Mian Hasnain Nawaz
This study reports the development of β-CD/GO and TMB based fluorescent sensing system synthesized via a facile hydrothermal approach for sensitive detection of ascorbic acid (AA) in food matrices. The structural and surface characteristics of such nanohybrid were confirmed by XRD, FTIR, Raman, zeta potential, and particle size analysis, while microscopic investigations revealed uniform surface modification of the nanosheets. In analytical studies, a fluorescence quenching based sensing mechanistic system was demonstrated through host guest molecular recognition and photoinduced electron transfer (PET) upon the introduction of AA. The synergistic integration of GO, and host guest recognition capability of β-CD, along with its strong quenching efficiency, enabled precise detection of AA. Under the optimized conditions, the sensor exhibited a linear response over the concentration range of 2.5–40 μM, with a detection limit of 1.21 μM. Furthermore, the developed probe was successfully applied to real food samples, including lemon, mango, and orange juices with recoveries of 92.3–106.4%. Overall, this work presents a rapid, cost-effective, and portable fluorescence-based strategy for practical food safety analysis.
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DOI
高影响因子
Food Analytical Methods
·
2023
·Vol. 16
·期 9
·1477-1486
·
IF 2.9
Liu Lei,Zhao Binyu,Luo Xiaogang,Meng Lingkui,Wu Fengshou
Porous organic polymers (POPs) are a class of materials that combine the properties of both polymers and porosity. They are characterized by their network structure composed of repeating organic units, which form pores or cavities throughout the material. Owing to their unique features including tunable porosity, abundant accessible active sites and synthetically control ability, POPs have been widely employed in the fields of catalysis, adsorption, and biomedicine. The incorporation of porphyrin building units into POPs endows POPs with unique structural characteristics and excellent optical properties. In this work, the aldehyde porphyrin with polyethylene glycol chain was successfully synthesized with good hydrophilicity, which was then reacted with tetrakis(4-aminophenyl) methane to construct the porphyrin-based porous organic polymers. The porphyrin-based POPs was then metallized to yield iron-containing porous organic polymers (Fe-POPs), which could catalyze the transformation of colorless substrate (3,3′,5,5′-tetramethylbenzidine (TMB)) to a blue product (ox-TMB) in the presence of hydrogen peroxide (H 2 O 2 ). Upon the addition of the ascorbic acid (AA) to the aforementioned system, the blue solution faded gradually to colorless. Based on this phenomenon, a rapid and sensitive colorimetric assay for the measurement of H 2 O 2 and AA was developed using Fe-POPs as mimetic enzyme. Fe-POPs was then successfully applied in real samples to detect AA with high accuracy. As a potential peroxidase mimic, Fe-POPs demonstrated the benefits of excellent stability and catalytic efficiency, which presented the promising application in the area of biocatalysis and biosensing. Graphical
研究领域:
材料科学
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化学生物学
·
分析化学
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生物化学
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纳米技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Wenwu Zhou,Yasong Zhou,Qiang Wei,Sijia Ding,Shujiao Jiang,Qing Zhang,Meifang Liu
Mesoporous Y composites which exhibit both crystallized micropores and large mesopores with narrow mesopore diameter distributions and controlled pore diameters were successfully synthesized. The composite is synthesized through an elaborate design of the SiO 2 @cationic surfactant cetyltrimethylammonium bromide (SiO 2 @CTAB) micelle swelled by an appropriate amount of 1,3,5-trimenthylbenzene (TMB); it is subsequently fabricated from the condensing of the Y zeolite precursors, which contain Y zeolite seeds, on the mesoscale micelle followed by a hydrothermal crystallization process. The effect of synthesis conditions such as the amounts of CTAB, TMB and TEOS added to the synthesis system on the physicochemical properties of the obtained products was examined. The as-synthesized materials were carefully characterized by employing XRD, N 2 adsorption–desorption, SEM, TEM and pyridine-adsorbed FTIR techniques. The mesoporous Y zeolite exhibited both the typical diffraction characteristics of Y zeolites in the wide-angle XRD pattern, and the typical diffraction characteristics of a narrowly dispersed mesopore structure in the small-angle XRD pattern is successfully synthesized for the first time. Finally, the adsorption capacity for DBT and 4,6-DMDBT by samples with different mesopore diameters were assessed by a batch method. Sample MY-0.5C-0.35T-0.9B showed the highest capacity for adsorbing DBT in model diesel, and sample MY-0.5C-0.35T-1.5B showed the highest capacity for adsorbing 4,6-DMDBT in model diesel. Most importantly, sample MY-0.5C-0.35T-1.5B showed the highest adsorptive rate for both DBT and 4,6-DMDBT, which is at least 10 times faster than that of sample MY-0C-0.35T-0B because of the elimination of the diffusion limitation.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 55
·36012-36017
·
IF 4.036
Ya Di,Jiyao Zheng,Yunwang Zhao,Zikai Yang,Changshun Xie,Jiahan Yu,Yue Zheng,Liming Gao
In this work, a novel strategy of colorimetric and photothermal dual-mode sensing determination of ascorbic acid (AA) based on a Ag+/3,3′,5,5′-tetramethylbenzidine (TMB) system was developed. In this sensing system, Ag+ could oxidize TMB with a distinct color change from colorless to blue color, strong absorbance at 652 nm and a photothermal effect under 808 nm laser irradiation due to the formation of oxidized TMB (oxTMB). When AA was present, oxTMB was reduced accompanied by a change from blue to colorless, and a decrease in absorption peak intensity and the photothermal effect. AA concentration showed a negative linear correlation with the value of both the absorbance intensity at 652 nm and temperature in the range of 0.2–10 μM (A = −0.03C + 0.343 (R2, 0.9887; LOD, 50 nM); ΔT = −0.57C + 8.453 (R2, 0.997; LOD, 7.8 nM)). Based on this, a sensing approach for detection of AA was proposed with dual-mode and without the complicated synthesis of nanomaterials. The photothermal effect and colorimetric signal provided a dual-mode detection strategy for AA, overcoming the limitations of any single mode. This colorimetric and photothermal dual-mode detection has great potential in the detection of AA in clinical pharmaceuticals and the construction of portable and highly sensitive sensors.
研究领域:
分析化学
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医学检测
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生物传感
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Miaomiao Song,Weihua Ma
Increasing attention has been directed towards oxidase mimics due to their catalytic ability toward enzyme substrates in the absence of H 2 O 2 , offering great colorimetric sensing potential in assays and diagnostics. However, slow electron-transfer and unstable absorbance over time often lead to unsatisfactory enzymatic reaction rates and unreliable readouts, significantly reducing detection efficiency and accuracy. Herein, we report on an Ag 6 Si 2 O 7 nanozyme utilizing 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogenic substrate, demonstrating excellent oxidase-like activity and long-term stable colorimetric signals. Experimental results reveal that the excellent catalytic kinetics of Ag 6 Si 2 O 7 (K m = 0.197 mM, Vmax = 5.1 × 10 −7 M/s) are attributed to ultrafast electron transfer between Ag + and TMB, mediated by Ag 2 O intermediates formed from self-released alkaline ions. Additionally, self-released H 2 SiO 4 2− improves the stability of the oxidized substrate, resulting in a long-term stable colorimetric signal (∼20 min), which exhibits reliable accuracy regardless of the time point at which analyte is added within 20 min, thus eliminating the interference of individual operation time errors. Ascorbic acid (AA) is selected as the target molecule to assess the performance of the Ag 6 Si 2 O 7 -TMB colorimetric sensing system. This approach demonstrates a wide detection range from 5 to 300 μM and a detection limit of 0.452 μM. The developed colorimetric sensing strategy exhibits good stability, specificity, rapidity, and potential application in real sample detection.
研究领域:
生物化学
·
分析化学
·
医学诊断
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产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Tang Shurong,Liu Qiao,Hu Jie,Chen Wei,An Fengping,Xu Hui,Song Hongbo,Wang Yi-Wei
In this paper, we developed a quick, economical and sensitive colorimetric strategy for copper ions (Cu2+) quantification via the redox response of MnO2 nanosheets with glutathione (GSH). This reaction consumed MnO2 nanosheets, which acted as a catalyst for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue product (oxTMB). In the presence of Cu2+, the GSH was catalyzed to GSSG (oxidized glutathione), and the solution changed from colorless to deep blue. Under the optimum conditions, the absorption signal of the oxidized product (oxTMB) became proportional to Cu2+ concentration in the range from 10 to 300 nM with a detection limit of 6.9 nM. This detection system showed high specificity for Cu2+. Moreover, the system has been efficaciously implemented for Cu2+ detection in actual tap water samples. The layered-nanostructures of MnO2 nanosheets make it possess high chemical and thermal stability. TMB can be quickly oxidized within 10 min by the catalyzing of MnO2 nanosheets with high oxidase-like activity. There is no need of expensive reagents, additional H2O2 and complicated modification processes during the colorimetric assay. Therefore, the strategy primarily based on MnO2 nanosheets is promising for real-time, rapid and highly sensitive detection of Cu2+ under practical conditions.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Shengmei Tai,Zhijuan Qian,Hongxin Ren,Alberta Osei Barimah,Chifang Peng,Xinlin Wei
A colorimetric assay based on an enzyme-inhibition strategy is promising for the on-site detection of pesticide residues . However, very few works of pesticide detection were reported based on the inhibition toward nanozymes although nanozymes have demonstrated many advantages in sensing various targets. Herein, a facile colorimetric detection for Glyp was developed based on β [email protected] enzyme mimics. The β [email protected] with high peroxidase-like activity was synthesized using random DNA double strands as template and β -CD as surface ligand. β [email protected] could catalyze the H 2 O 2 -3,3′,5,5′-tetramethylbenzidine (TMB) system. The oxidation product OxTMB with a blue color and presented a large absorption signal at 652 nm. However, Glyp could destroy the synergic effect between redox doublet (Cu 2+ /Cu + ) on the β [email protected] A-CuNCs surface, resulting in the inhibition of the peroxidase-like activity. Colorimetric detection for Glyp could be established by detecting the changes of absorption signal at 652 nm. The linear range was 0.02–2 μ g/mL and the detection limit was 0.85 ng/mL (3δ/s). The method was applied in measuring Glyp spiked in lake water and various food samples. This method had rapidness, high sensitivity, and selectivity advantages, indicating the high application potential in monitoring Glyp residue in food.
研究领域:
分析化学
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环境科学
·
食品安全
·
农业化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 21
·6494
·
IF 4.412
Lianlian Fu,Deshuai Yu,Dijuan Zou,Hao Qian,Youhui Lin
Recently, the design and development of nanozyme-based logic gates have received much attention. In this work, by engineering the stability of the nanozyme-catalyzed product, we demonstrated that the chromogenic system of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) can act as a visual output signal for constructing various Boolean logic operations. Specifically, cerium oxide or ferroferric oxide-based nanozymes can catalyze the oxidation of colorless TMB to a blue color product (oxTMB). The blue-colored solution of oxTMB could become colorless by some reductants, including the reduced transition state of glucose oxidase and xanthine oxidase. As a result, by combining biocatalytic reactions, the color change of oxTMB could be controlled logically. In our logic systems, glucose oxidase, β-galactosidase, and xanthine oxidase acted as inputs, and the state of oxTMB solution was used as an output. The logic operation produced a colored solution as the readout signal, which was easily distinguished with the naked eye. More importantly, the study of such a decolorization process allows the transformation of previously designed AND and OR logic gates into NAND and NOR gates. We propose that this work may push forward the design of novel nanozyme-based biological gates and help us further understand complex physiological pathways in living systems.
研究领域:
生物化学
·
纳米生物学
·
分子生物学
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DOI
高影响因子
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