TRANSPLANTATION
·
2005
·Vol. 80
·期 8
·1003
·
IF 3.568
Sweet Ian R.,Gilbert Merle,Jensen Rich,Sabek Omaima,Fraga Daniel W.,Gaber Ahmed O.,Reems JoAnna
Background. An in vitro method to assess human islets could prevent transplantation of nonviable islets and facilitate the optimization of islet preparation. We hypothesize that glucose-stimulated cytochrome c reduction and oxygen consumption by human islets can be used as predictors of transplant success. Methods. Isolated human islets were obtained from research-grade pancreata. Using a previously developed islet flow culture system, the response of cytochrome c reduction and oxygen consumption to glucose was compared to the ability of islets transplanted into nondiabetic NOD-SCID mice to secrete C-peptide in response to a glucose tolerance test conducted 7 days following transplant (n=10). Results. In vitro responses by human islets were qualitatively similar to those seen in rat islets: glucose increased both oxygen consumption and cytochrome c reduction. However, the responses were smaller in magnitude and quite variable. Scatter plots of C-peptide and quantiles for ln(C-peptide) indicated that 12 ng/ml could be used as threshold of transplant success with which to evaluate the diagnostic potential of cytochrome c and oxygen consumption. Data was analyzed by generating receiver operating curves and the area under the curve was 0.889 (95% CI: 0.645–1.000) and 0.738 (95% CI: 0.413–1.000) for cytochrome c reuction and oxygen consumption respectively (1 indicates absolute predictive capability and 0.5 indicates no predictive capability). Conclusions. The detection of glucose-stimulated cytochrome c reduction and oxygen consumption may have utility as criteria for the assessment of human islet quality.
研究领域:
生物医学工程
·
内分泌学
✓ 已验证
DOI
PubMed
高影响因子
Translational Pediatrics
·
2025
·Vol. 14
·期 8
·2023
·
IF 1.7
He Mingxin,Liu Lei,Li Yue,Guo Zhi
The initial bacteria in a newborn’s gastrointestinal tract come from the mother’s birth canal, the environment, and breast milk. Gut microbiota is established earlier in infants delivered naturally than by cesarean section (C-section). Gut microbial diversity increases further as infants reach weaning, with the addition of complementary foods and increased food diversity. Upon reaching adulthood, the composition of the microbiota is relatively stable, with Firmicutes , Bacteroides and Actinomyces as the main microbiota. The development of gut microbiota in children, as well as the potential disruption of newborn gut microbiota development by antibiotics, necessitates the development of new nutritional foods to address the challenges of gut microbiota and nutrition in children. Specific probiotic strains may prevent acute gastroenteritis, antibiotic diarrhea, infantile colic, and necrotising enterocolitis (NEC). Microecological preparations such as probiotics are a group of live microorganisms that provide health benefits to the host when given in sufficient quantities, and are mainly categorized into live bacteria in bulk, tablets and capsules. Probiotics are currently widely used in the treatment of gastrointestinal related diseases, and have achieved certain results in clinical practice as a supplement to conventional therapies. However, there are still not many types of microecological preparations that have isolated strains. Finding probiotics that target a particular disease and isolating them is a difficult part of the development of microecological preparations for clinical applications. At present, in the clinical cohort study of children using probiotic preparations, microecological preparations either show their ability to prevent diseases or are mostly used as auxiliary means of traditional therapy. Although there are fewer clinical cohort studies with microecological preparations as the primary treatment, their clinical application will be increasingly promising. This review aims to systematically summarize the formation patterns of gut microbiota in children, analyze the mechanisms of action of microecological regulators, and evaluate their clinical applications in pediatric diseases, thereby providing insights for addressing gut microbiota-related challenges.
✓ 已验证
DOI
PubMed
高影响因子
Translational Pediatrics
·
2021
·Vol. 10
·期 4
·860
·
IF 2.488
Yang Shen,Jing Zhao,Ling Sun,Li Zeng,Zhi Chen,Mi Tian,Xingtao Zhou
Background The syndrome of monocular myelinated retinal nerve fibers (MRNF), myopia and amblyopia threatens visual development of infants and young children. The efficacy of part-time occlusion therapy remains controversial, and the long-term prognosis of the syndrome remains unclear. Methods Ten children (4.40±2.22 years, 4 boys and 6 girls) with monocular MRNF, myopia and amblyopia were recruited. Both the affected eyes (treatment group) and the fellow eyes (control group) underwent routine ophthalmic examinations, including AL, cycloplegic refraction, best-spectacles-corrected distance visual acuity (BSCDVA), cover test, simultaneous perception, corneal curvature, anterior segment and fundus examinations. Refractive error was corrected by either spectacles or rigid gas permeable (RGP) contact lenses. Part-time occlusion therapy was employed for amblyopia treatment. The retinal characteristics were examined only on the final visit with a Fourier-domain optical coherence tomography (FD-OCT). The speed of myopia progression and axial elongation were calculated. Statistical analysis was performed using SPSS 20 software. Cut-off P values were 0.05. Results On the final visit, six children had normal simultaneous perception but three had strabismus. The mean AL and spherical equivalent (SE) values of the affected eyes were 28.05±1.59 mm and −12.60±4.20 D, respectively, while those of the fellow eyes were 23.67±1.13 mm and −0.66±1.99 D, respectively. AL and SE were significantly different over time and between groups (P<0.0001), but no time*group interaction effect (P>0.05) was observed. The differences in the corneal curvature, mean speed of myopia progression, axial elongation, foveal thickness and parafoveal thickness between the two groups were not significant (P>0.05). However, the perifoveal thickness in the affected eyes was significantly higher than that of the control eyes (P=0.047). In the treatment group, the occlusion dosage positively correlated with the final BSCDVA (R=0.764, P=0.016) but did not correlate with the speed of myopia progression or axial elongation (both P values >0.05). Conclusions The affected eyes have longer AL, severer myopia and thicker perifoveal retina when compared with the fellow eyes. Both the affected and the fellow eyes have similar speed of myopia progression as well as that of axial elongation.
研究领域:
眼科
·
小儿眼科学
·
屈光学
·
视觉发育
·
影像学
·
临床研究
✓ 已验证
DOI
PubMed
高影响因子
Translational Pediatrics
·
2021
·Vol. 10
·期 9
·2313
·
IF 2.488
Wei Shen,Wen Qiu,Yuting Liu,Weihua Liao,Yiyi Ma,Yan He,Zhang Wang,Hongwei Zhou
Background The gut microbiome plays a potential role in clinical events in preterm infants and may affect their lateral development. Understanding the initial colonization of microbes in the gut, their early dynamic changes, and the major factors correlated with these changes would provide crucial information about the developmental process in early life. Methods The present study enrolled 151 preterm infants and examined the longitudinal dynamics of their fecal microbiome profiles during the period of hospitalization using 16S ribosomal RNA gene sequencing. Random forest modeling was used to predict postnatal age (Age), postmenstrual age (PMA), and gestational age (GA), using gut microbiome features. Results Principal coordinate analysis revealed that the gut microbiome of the preterm infants displayed an obvious time-dependent change pattern, which showed the strongest association with Age, followed by PMA, and a much weaker association with (GA). Random forest modeling further evidenced the time-dependent change pattern, with the Pearson’s correlation coefficients between the actual values and the gut microbiome-predicted values being 0.68, 0.53, and 0.38 for postnatal, postmenstrual, and gestational age, respectively. The microbiome dynamism could be further divided into four Age stages, each with its own characteristic microbial taxa. The first 1–4 days (T1 stage) represented the meconium microbiome, with colonization of a high diversity of microbes before or during delivery. During 5–15 days (T2 stage), the gut microbiome of the preterm infants underwent a rapid turnover, in which microbial diversity declined, and stabilized afterward. Enterobacteriaceae , Enterococcaceae , Streptococcaceae , Staphylococcaceae , and Clostridiaceae were the major classes in the gut microbiome in the lateral stages of development (T3–T4 stage). Conclusions Postnatal age, rather than the gestational age, is significantly correlated with the gut microbiome of preterm infants, suggesting that clinical interventions contribute more to the early dynamics of gut microbiome in preterm infants than the natural development of the gut.
研究领域:
微生物组学
·
新生儿科学
·
发育生物学
·
临床微生物学
·
生物信息学
✓ 已验证
DOI
PubMed
高影响因子
Translational Pediatrics
·
2025
·Vol. 14
·期 6
·1213
·
IF 1.7
Zihong Shan,Chenghui He,Qingyao Yang,Jingjing Xiong,Meng Li,Mingyao Meng,Yongkun Huang
Background Previous research has indicated that chenodeoxycholic acid (CDCA) plays a significant role in intestinal motility and is implicated in some gastrointestinal (GI) disorders. However, the impact of CDCA on defecation patterns in infants, particularly those who are breastfed, remains unclear. This study explored the relationship between CDCA and defecation frequency in breastfed infants, focusing on the underlying mechanisms. Methods We measured CDCA levels in stool samples of infants with varying defecation frequencies using metabolomics, along with studies to explore the impact of CDCA on murine colonic transit and with in vitro cellular studies to elucidate the underlying physiological mechanisms. Results In our murine model, CDCA treatment increased GI transit distance, enhanced fecal water content, and reduced transit time, without causing significant colonic damage. Additionally, CDCA treatment enhanced 5-hydroxytryptamine (5-HT) secretion and upregulated signaling molecules such as tryptophan hydroxylase 1 (Tph1), G protein-coupled bile acid receptor 1 (TGR5), and transient receptor potential ankyrin 1 (TRPA1). Selective inhibition of these pathways suppressed 5-HT secretion and normalized intestinal motility parameters, confirming their mechanistic role. Moreover, CDCA treatment of rat insulinoma-derived RIN-14B cells also resulted in increased 5-HT secretion. Conclusions These findings reveal that CDCA significantly correlates with defecation frequency in breastfed infants and likely influences intestinal motility through the TGR5/TRPA1-5-HT signaling axis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMBUSTION SCIENCE AND TECHNOLOGY
·
2025
·
IF 1.5
Chunyue Zhang,Feng Zhang,Jiaji Cheng,Yapeng Wang,Zhongan Jiang
In order to solve the problem of coal mine fires caused by spontaneous combustion of coal, which often occurs during coal production, a new type of tea polyphenol-melamine formaldehyde resin-soy protein isolate (TP-MF-SPI) flame-retardant microcapsule was prepared by in situ polymerization in this paper. This is a flame-retardant microcapsule with tea polyphenol (TP) and MgCl2 as the core material and soy protein isolate (SPI)-modified diamine resin as the wall material. When the coal samples were oxidized and decomposed rapidly due to the temperature increase and slowly entered into the combustion stage, the coal samples with added core and microencapsulated materials have increased several important temperature points compared with the original coal. From the simultaneous thermal analyzer experiments, it was concluded that the temperature point of the mass maxima by 1.3℃ and 4.7℃, the ignition temperatures by 1.6℃ and 4.1℃, and the temperature point of the maximal loss rate by 3.7℃ and 12.3℃. It can be seen that the use of microencapsulation technology for magnesium chloride-tea polyphenol coating can be more effective in prolonging the flame-retardant life of the core material in the high temperature stage of the coal samples, and to enhance its flame-retardant effects. The performance test of microencapsulated flame retardant using cone calorimeter experiment and thermal stability analysis experiment showed that the peak heat release rate of the original coal samples and the coal samples with 1:5, 1:4, 1:3 flame retardant additions were 72.54 kW/m2, 55.17 kW/m2, 35.99 kW/m2, 30.31 kW/m2. Through the different ratios of microcapsule material coal samples show the effect of flame retardant effect and the cost of a comprehensive comparison and analysis, to determine the 1:4 microcapsule material additive amount can play the best flame retardant effect on the coal sample.
✓ 已验证
DOI
高影响因子
Water
·
2025
·Vol. 17
·期 2
·179
·
IF 3
Wei Xu,Xiaoping Zhang,Guichang Zhang,Xin Zhang
The treatment of wastewater using microalgae is regarded as a green and potential technology. However, its engineering application has been largely hindered because of the limitation of microalgae separation and harvesting. Therefore, immobilization technology has been widely used to embed microalgae for wastewater treatment. In this paper, sodium alginate (SA) and polyvinyl alcohol (PVA) as the common immobilized carriers were used to immobilizeankistrodesmus falcatusfor simulated slaughtering wastewater (SSW) treatment. The experimental results of the mass transfer and adsorption of immobilized carriers were found to show that the mass transfer of SA-SiO2gel balls (SS-GB) was better than PVA-SA gel balls (PS-GB) and that the adsorption of PS-GB was better than SS-GB. When immobilizing microalgae with the two kinds of carriers, it was found that SA-SiO2microalgal spheres (SS-MS) were better than PVA-SA microalgal spheres (PS-MS) for the maintenance of microalgal cell activity and that PS-MS were better than SS-MS for the resistance to biodegradation. This is because the carrier of PS-MS had a thick shell and dense structure, while the carrier of SS-MS had a thin shell and loose structure. The results of SSW treatment by PS-MS and SS-MS were found to show that the total phosphorus (TP) removal rates of PS-MS and SS-MS were 90.31% and 86.60%, respectively. This indicates that the TP removal effect of PS-MS was superior to that of SS-MS. The adsorption kinetics simulation showed that the adsorption of TP onto PS-GB was controlled by chemisorption and that the adsorption of TP onto SS-GB was controlled by physical adsorption. The chemical oxygen demand (COD) and ammonium nitrogen (NH4+-N) removal of PS-MS were 9.30% and 10.70%, respectively, and the COD and NH4+-N removal of SS-MS were 54.60% and 62.08%, respectively. This indicates that the COD and NH4+-N removal effect of SS-MS were superior to PS-MS. This is the result of the combined action of the degradation by microalgal cells and adsorption by the carrier.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2022
·Vol. 12
·期 10
·3158-3164
·
IF 6.177
Ping Xue,Xin Pan,Tian Tian,Mi Tang,Wei Guo,Junsheng Li,Zhengbang Wang,Haolin Tang
Covalent organic frameworks (COFs) as new photocatalysts for hydrogen evolution have aroused considerable interest over the past few years. However, most of COF-based photocatalysts require expensive and rare Pt co-catalysts to achieve a high hydrogen evolution rate. Here, a series of Tp-Pa-2-COF-based photocatalyst materials with porous 2D conductive metal–organic framework (MOF) Cu3(HHTP)2 as a noble metal-free co-catalyst are successfully prepared by an easy handling and green mechano-chemical method. Remarkably, after optimizing the ratio of the COF and MOF, the Tp-Pa-2/Cu3(HHTP)2 hybrid composite with a mass ratio of 3 : 1 exhibits the highest hydrogen evolution rate of 7.71 mmol h−1 g−1 under visible-light irradiation, while the hydrogen evolution rate of the pure Tp-Pa-2 is only 0.026 mmol h−1 g−1. Based on the detailed experiments and calculations, the improved performance can be mainly explained by the porous crystalline Cu3(HHTP)2 co-catalyst in the resulting composites efficiently promoting the utilization rate of the photogenerated charge-carriers, thus contributing to the high hydrogen generation performance.
研究领域:
材料科学
·
光催化
✓ 已验证
DOI
高影响因子
ARCHIVES OF MICROBIOLOGY
·
2025
·Vol. 208
·期 1
·1
·
IF 2.6
Zhong Huichang,Chen Liyi,Liu Weifeng,Tao Yong
Xanthophyllomyces dendrorhous (anamorph form Phaffia rhodozyma ) is a key microbial host for industrial astaxanthin production, where yield depends on both the efficiency of the biosynthetic pathway and overall metabolic robustness. To develop superior industrial strains, we established an efficient evolutionary-enrichment system and an accelerated evolutionary workflow that couples astaxanthin production with cell growth under optimized oxidative stress conditions. This strategy enabled the enrichment of mutants exhibiting both high astaxanthin production and strong industrial fitness. Using this approach, we employed a high astaxanthin-producing X. dendrorhous strain A06 as the parental strain for evolution. After several rounds of screening over 2,000 colonies, we successfully identified the top-performing mutant, TP-4. TP-4 demonstrated a stable 20% increase in astaxanthin production relative to the parental strain, along with faster growth and enhanced fermentation performance. The genome of TP-4 was sequenced, and comparative genomic analysis against the parent strain A06 and a low-astaxanthin-producing control strain, C01, revealed multiple mutations potentially underlying its superior phenotype. These findings provide new insights into the metabolic and genetic basis of astaxanthin overproduction. This work establishes an efficient evolutionary platform for strain improvement and provides a mechanistic understanding of astaxanthin overproduction in X. dendrorhous , laying the groundwork for further optimization and industrial-scale application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2022
·Vol. 24
·期 19
·3630-3636
·
IF 3.756
Ding-Chong Han,Yu-Hui Tan,Jia-Hui Wen,Yun-Zhi Tang,Peng-Fei Wu,Yu-Kong Li,Ming-Yang Wan,Xiao-Wei Fan
Materials combining high-temperature phase transformation and fluorescence properties are not abundant, especially, stator–rotator-type inclusion compounds possessing excellent optical properties are extremely rare. In this study, a neoteric high-temperature crown ether inclusion luminophor: [(2,4,6-trifluoroanilinium)-(18-crown-6)][BF4] (1) was constructed, undergoing a reversible phase transition near Tp = 364 K. Differential thermal analysis (DTA), specific heat capacity (Cp) and differential scanning calorimetry (DSC) tests disclosed a reversible first-order phase transformation behavior of 1. Multiple-temperature PXRD analyses further demonstrated a structural transition from low- to high-symmetry. Permittivity tests for 1 exhibited a prominent step-like dielectric response near Tp, indicating that it is a promising dielectric switching material. Besides, 1 occupies a broad optical energy band of ca. 4.45 eV. The photoluminescence tests for 1 display a strong luminescence emission peak at 368 nm. Further research and computational analysis showed that the luminescence lifetime of 1 reaches up to 43.998 μs. The investigation opens up an alternative avenue to construct and synthesize phase-transition compounds possessing high-Tp and superior optical properties.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 16
·2878
·
IF 5.1
Bochen Jiang,Xiaojing Sui,Ziran Niu,Yanhua Lei,Jie Wang,Liu Hui,Bing Zhang,Jiangdong Cao,Yu Gu,Zhongzhou Cui
Solar-driven advanced oxidation processes and interfacial water evaporation represent promising strategies to simultaneously address water pollution and freshwater scarcity. However, conventional photo-Fenton catalysts are predominantly powder-based and suffer from difficult catalyst recovery, inefficient H2O2utilization, and limited practical applicability. Herein, a multifunctional bacterial-cellulose-based composite membrane (BC-MTP) was designed by immobilizing 1T-2H MoS2and a tea-polyphenol/polypyrrole (TP/PPy) copolymer through a blending–copolymerization strategy. The three-dimensional BC network anchors the catalytic components and supports their repeated use, while the BC-MTP membrane achieves 88.2% tetracycline removal using 20 μL H2O2, four times lower than the dosage used for the corresponding powder catalyst. Because dissolved Fe was not quantified by ICP-OES or ICP-MS, the present results are interpreted as evidence of improved H2O2utilization and component immobilization rather than quantitative proof of suppressed iron leaching. The incorporation of 1T-2H MoS2and TP/PPy also provides strong broadband light absorption and photothermal conversion, yielding a solar-driven evaporation rate of 1.34 kg m−2h−1and an energy efficiency of 80.1% under the tested laboratory conditions. This work demonstrates a membrane platform integrating photo-Fenton degradation and solar-driven water evaporation for water-purification applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Guiyun Deng,Yang Wu,Zhiyong Song,Shuojun Li,Moqing Du,Jiamin Deng,Quan Xu,Liu Deng,Hagar Shendy Bahlol,Heyou Han
Infection with Helicobacter pylori (Hp) is one of the leading causes of stomach cancer. The ability to treat Hp infection is hampered by a lack of stomach gastric acid environment. This work introduces a nanoliposome that can rapidly adjust the gastric acid environment to ensure a drug’s optimal efficacy. We introduce CaCO3@Fe–TP@EggPC nanoliposomes (CTE NLs) that are composed of Fe3+ and tea polyphenols (TPs) forming complexes on the surface of internal CaCO3 and then with lecithin producing a phospholipid bilayer on the polyphenols’ outer surface. Through the action of iron–TP chelate, the phospholipid layer can fuse with the bacterial membrane to eliminate Hp. Furthermore, CaCO3 can promptly consume the excessive gastric acid, ensuring an ideal operating environment for the chelate. TPs, on the other hand, can improve the inflammation and gut microbes in the body. The experimental results show that CTE NLs can quickly consume protons in the stomach and reduce the bacterial burden by 1.2 orders of magnitude while reducing the inflammatory factors in the body. The biosafety evaluation revealed that nanoliposomes have good biocompatibility and provide a new strategy for treating Hp infection.
研究领域:
药学
·
纳米技术
·
微生物学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）