MICROSCOPY RESEARCH AND TECHNIQUE
·
2021
·Vol. 85
·期 4
·1518-1526
·
IF 2.769
Leilei Hong,Lichan Yuan,Xiaoxu Xu,Yuhuan Ma,Li Meng,Junyi Wang,Na Zhao,Xiaoliang Wang,Junqing Ma
Titanium (Ti) alloys, particularly Ti 6 Al 4 V, are the most commonly used biomedical implant material. Ti alloys are biologically inert, so there have been continuous efforts to improve their osteogenic properties and clinical performance. Since TiO 2 nanotubes (NT) appear to be excellent drug platforms, and strontium reportedly enhances osteogenesis, we constructed a TiO 2 nanotube coating on the surface of Ti 6 Al 4 V and immersed it in Sr (OH) 2 solution in order to incorporate Sr into TiO 2 nanotubes (NT-Sr). The results of field emission scanning electron microscope and X-ray diffraction analysis verified the fabrication of NT-Sr. We next added polydopamine (PDA) and cyclo- (arginine–glycine–aspartic acid–phenylalanine–cysteine) [c(RGDfC)] peptides to further promote biocompatibility of the implant. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirmed the existence of PDA and c(RGDfC). Mesenchymal stem cells (MSCs) were planted on Ti, NT, NT-Sr, NT-Sr/PDA, and NT-Sr/PDA-RGD surfaces. The adhesion and differentiation of MSCs on different surfaces were evaluated. The mRNA expression of alkaline phosphatase, runt-related transcription factor 2 (Runx2) and type I collagen (Col I) of different groups were also tested. Finally, we observed that the NT-Sr/PDA-RGD group showed significantly better performance than other groups in terms of the differentiation and osteogenesis-related gene expression of MSCs. Thus, the NT-Sr/PDA-RGD complex may be an important modification strategy for Ti, as it shows excellent osteogenic potential.
研究领域:
生物材料
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组织工程
·
骨科
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Lingling Cui,Hang Li,Jiewen Huang,Dangsheng Xiong
To improve the biotribological properties of Ti6Al4V alloy for artificial joints, motivated by the composite structure of ‘natural articular cartilage - subchondral bone’ and the hydration lubrication mechanism of articular cartilage, a soft/hard bilayer coating composed of a porous TiO 2 layer and a zwitterionic hydrogel coating was designed and fabricated on Ti6Al4V alloy through micro-arc oxidation (MAO) combined with ultraviolet (UV) radiation technology. Characterization results revealed that the bio-inspired bilayer coating could be successfully constructed on Ti6Al4V alloy, and such a coating displayed good hydrophilicity (WCA: 32°). Notably, by ball - on - disk friction test, this biomimetic bilayer coating exhibited an ultra-low friction coefficient (∼0.038) in PBS solution, which was reduced by an order of magnitude compared with that of the Ti6Al4V alloy substrate. This was due to the synergistic effects of soft/hard composite structure and the hydration lubrication of zwitterionic MPC polymer. This work provides a novel and effective strategy for surface modification of Ti6Al4V alloy for artificial joints.
研究领域:
生物材料
·
表面工程
·
摩擦学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2025
·
IF 6.1
Ye Wang,Zhengxuan Hu,Guolong Wu,Xiaonan Zhang,Yanyi Yin,Lin Li,Jianhua Yao
In this study, laser-assisted micro-arc oxidation (LMAO) processing was carried out on Ti6Al4V alloy under laser powers of 30, 45, and 60 W, and the influence of the laser power on the microstructure and wear resistance of the coating was further investigated. The breakdown voltage and termination voltages of LMAO processing decrease gradually as the laser power increases. At the same time, micro-discharges increase in quantity. Within the suitable laser power range (30–45 W), the obtained coating continuously enhances uniformity, thickness, and density with rising power, contributing to higher hardness and adhesion strength . However, raising laser power to 60 W will damage the obtained coating due to the violent micro-discharges, which generate interconnected larger pores in the local area. Laser power also affects the coating's reaction process and phase transition. More electrolyte ions dope into the coating as a consequence of increasing laser power. The Si concentration of the LMAO-45 coating is the highest, whereas that of the LMAO-60 coating comparatively reduces due to localized coating detachment. Moreover, enhancing laser power is more conducive to forming SiO 2 and rutile-TiO 2 phases. Compared with the traditional MAO or other LMAO coatings, the LMAO-45 coating significantly improves wear protection, evidenced by the lowest COF value , the minimum wear rate, and the least peeling during the wear test . Low porosity, surface roughness , high thickness, and increased incorporation of the SiO 2 phase are responsible for the same. However, under 60 W of laser irradiation , the wear resistance of the resultant LMAO coating decreased due to the formation of local damaged areas.
研究领域:
材料科学
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表面工程
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生物医学工程
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生物材料
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机械工程
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金属腐蚀与防护
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纳米材料
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医用植入材料
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产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Lingling Cui,Hang Li,Chenyang Gong,Jiewen Huang,Dangsheng Xiong
Titanium alloys with excellent mechanical properties and biocompatibility are widely used in surgical implants. However, due to its poor tribological properties, it is difficult to be used in joint bearing interfaces. Herein, mimicking the soft/hard hierarchical structure of articular cartilage/subchondral bone and the unique lubrication mechanisms of articular cartilage, a biomimetic bilayer coating consisting of a TiO 2 layer and a hydrogel layer was fabricated on laser-textured Ti6Al4V alloy by laser surface texturing, thermal oxidation and ultraviolet radiation techniques. Meanwhile, a zwitterionic polymer (SBMA) was introduced into the hydrogel layer to provide hydration lubrication. The experimental results demonstrated that the introduction of SBMA greatly improved the hydrophilicity and compressive modulus of the hydrogel layer. Compared with Ti6Al4V, the Ti6Al4V-hydrogel bearing interface exhibited a lower friction coefficient (0.06) and better wear resistance when lubricated in deionized water. More importantly, this Ti6Al4V-hydrogel bearing interface could maintain stable and low friction coefficients when lubricated in different physiological solutions for a long-term friction (4 h), and the lowest friction coefficient (0.039) was measured in phosphate buffer (PBS) solution. The outstanding biotribological performance was mainly attributed to the biphasic and hydration lubrication mechanisms of the hydrogel layer. This study provides new insights into the development of Ti6Al4V bearing interfaces for artificial joints.
研究领域:
材料科学
·
生物医学工程
·
表面工程
✓ 已验证
产品关联
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2019
·
IF 3.192
Tong Zhou,Jie Liu,Xinwen Zhang,Bin Shen,Jinlong Yang,Wenbin Hu,Lei Liu
The antibacterial properties of the microarc oxidation coating have gained wide attentions, and W-containing surface has been verified to be notably bactericidal. Herein, in this work, doping Na 2 WO 4 into the electrolyte to prepare the antibacterial W-containing microarc oxidation coating is proposed for the first time. No obvious crystal phase related to hexavalent W exists in the coating, and sodium tungstate may transform into a trace amount of tungsten oxides and amorphous substances. The sodium tungstate plays a crucial role in the antibacterial capability of the coating, the W-containing coating exhibits conspicuous bactericidal performance against both planktonic and adherent Escherichia coli and Staphylococcus aureus . The antibacterial mechanism of the W-containing coating is first revealed to be linked to the extracellular and intracellular reactive oxygen species (ROS). The W-containing dissolved matters spontaneously trigger the formation of the extracellular ROS, which directly destroy the bacterial cytoderm and cytomembrane. Meanwhile, the nanoscale W-containing dissolved matters penetrate the cell wall, and stimulate the generation of intracellular ROS which interact with the cytoplasmic components. Ultimately, the bacteria die from the cooperative effects of the extracellular and intracellular ROS.
研究领域:
材料科学
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生物材料
·
表面工程
·
生物医学工程
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2015
·
IF 3.088
Huan Zhou,Shiqin Kong,Yan Pan,Zhiguo Zhang,Linhong Deng
Strontium has been shown to be a beneficial dopant to calcium phosphates when incorporated at nontoxic level. In the present work we studied the possibility of solution derived doping strontium into calcium phosphate coatings on titanium alloy Ti6Al4V based implants by a recently reported microwave-assisted method. By using this method strontium doped calcium phosphate nuclei were deposited to pretreated titanium alloy surface dot by dot to compose a crack-free coating layer. The presence of strontium in solution led to reduced roughness of the coating and finer nucleus size formed. In vitro study found that proliferation and differentiation of osteoblast cells seeded on the coating were influenced by strontium content in coatings, showing an increasing followed by a decreasing behavior with increasing substitution of calcium by strontium. It is suggested that this new microwave-assisted strontium doped calcium phosphate coatings may have great potential in implant modification.
研究领域:
生物医学工程
·
材料科学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Coatings
·
2026
·Vol. 16
·期 3
·297
·
IF 3.4
Lingling Cui,Guang Ji,Tongchun Qin,Zhiwei Li,Yan Sheng,Haiqin Ding,Guodong Jia
Traditional artificial joints mainly face the challenges of severe wear and aseptic loosening, which limits their application as joint bearing interfaces under high-stress loading conditions. To improve this problem, inspired by the gradient modulus structure of natural cartilage/subchondral bone and the inherent negative charge characteristics of the surface, a negatively charged hydrogel layer was adhered to a porous Ti6Al4V surface through a combination of ultraviolet irradiation and freeze–thaw cycles. The cross-sectional SEM image exhibited that the hydrogel layer was closely bonded to the hard substrate. After physical doping with SBMA, the lubrication performance of the composite bearing interface was significantly improved, primarily attributable to the biphasic lubrication of the hydrogel layer and the hydration lubrication mechanism of SBMA.
✓ 已验证
产品关联
DOI
高影响因子
Bio-Design and Manufacturing
·
2026
·
IF 7.9
Yue Xiang,Yang Wenbo,Chen Hao,Gao Xiaofan,Shang Jing,Song Lanfeng,Jia Shipu,Cao Lanqing,Lu Shiwei,Yao Rui,Fang Yuchi,Deng Fangbin,Han Qing,Zhao Xin,Wang Jincheng,Bai Haotian
Ti6Al4V implants are used to repair critical-sized bone defects, but their bioinert surfaces often cause insufficient early osteogenic stimulation, potentially leading to compromised long-term fixation. The osteogenic microenvironment is vital for osseointegration between the host bone and the implant and influences long-term clinical outcomes. Consequently, researchers have attempted to mimic the natural osteogenic microenvironment using microporous design, physical and chemical modification, and loading of bioactive factors. The present study proposes a strategy for fully recapitulating the natural osteogenic microenvironment within microporous Ti6Al4V implants. First, Ti6Al4V scaffolds were implanted into rat femoral condyle defects to establish osseointegration. The resulting construct was then demineralized and decellularized to produce an implantable Ti6Al4V/xenogeneic bone composite. Pull-out tests demonstrated that the bone tissue remained well-integrated with the scaffold after processing. Nucleic acid detection and galactose-α-1,3-galactose (α-Gal) analysis verified the elimination of immunogenicity in the composite. Immunofluorescence staining for bone morphogenetic protein-2 and transforming growth factor-β confirmed the preservation of key bioactive factors. In vitro cell experiments revealed favorable biocompatibility and osteoinductive activity. When implanted into rabbit femoral condyle defects, the composite significantly enhanced bone ingrowth into the microporous Ti6Al4V implant and strengthened osseointegration. Histological and quantitative analyses further showed that the composite shifted the osteogenic pattern from the osteoconduction-dominant mode characteristic of Ti6Al4V implants to an osteoinduction-dominant pattern of bone formation. In summary, we developed a novel Ti6Al4V/xenogeneic bone composite that fully recreated the natural osteogenic microenvironment. This composite effectively improved the osseointegration capacity of conventional scaffolds and demonstrated considerable potential for clinical application.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Hailun Xu,Qihao Chai,Xianxing Xu,Ziyang Li,Wenfei Bao,Zhentao Man,Wei Li
Periprosthetic bone defects are the most serious problem of revision total hip arthroplasty, which can easily lead to insufficient osteointegration between the prosthesis and host bone. Bone marrow mesenchymal stem cells (BMSCs) and a moderate inflammatory response at the prosthesis–bone interface play an important role in osteointegration. Here, we developed microarc oxide titanium implant loaded engineered exosomes (S-Exos) to promote osseointegration at the prosthesis–bone interface. First, Smurf1-shRNA was transferred into the BMSCs using a viral vector to prepare S-Exos, which were subsequently immobilized to the microarc oxide titanium implant surface with positively charged polyethyleneimine. The immobilized S-Exos could be slowly and uniformly released and subsequently phagocytosed by BMSCs and macrophages. Once the S-Exos were phagocytosed, they could simultaneously activate the BMP/Smad signaling pathway in the BMSCs and promote macrophage M2 polarization, both of which enhance osseointegration. Specifically, this S-Exos coating exhibits a dual effect of promoting osseointegration, including the osseointegration of BMSCs by activating the BMP/Smad signaling pathway and the macrophage M2 polarization promoting osseointegration. In summary, the construction of S-Exos modified microarc oxide titanium implants could provide a new method for promoting osteointegration between the prosthesis and host bone in revision total hip arthroplasty.
研究领域:
生物医学工程
·
骨科手术
·
组织工程
·
再生医学
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细胞生物学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Shangyong Zuo,Qian Peng,Tong Zhang,Ting Luo,Yuehong Wang,Zhiwei Peng
Boronized Ti6Al4V/FHA composites were prepared by microwave sintering of mixed powders of Ti6Al4V, TiB 2 , and synthesized fluorine-substituted hydroxyapatite (FHA) with variable compositions at 1050 °C for 30 min. The unique “lens effect” of microwave facilitated the in-situ transformation of nanoscale TiB 2 into TiB, serving as the reinforcing phase within the composites. Increasing the theoretical fluorine substitution in FHA from 0% to 50% caused less thermal decomposition of hydroxyapatite during sintering, promoting densification and thereby mechanical properties of the composites. However, continuous fluorine substitution to 100% led to excessive grain growth and FHA agglomeration, deteriorating the composite mechanical performance. The boronized Ti6Al4V/FHA composite produced using FHA with the theoretical fluorine substitution of 50% exhibited the highest compressive strength (397.7 MPa), proper compressive modulus (10.65 GPa) and maximum microhardness (385.3 HV). Furthermore, it showed excellent hydrophilicity and good cell adhesion, confirming its superior bioactivity for dental applications.
研究领域:
材料科学
·
牙科医学
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2023
·Vol. 16
·期 15
·5206
·
IF 3.4
Guijun Yang,Houjiang Liu,Ang Li,Tiansheng Liu,Qiqin Lu,Fang He
Titanium alloy Ti6Al4V is a commonly used bone implant material, primarily prepared as a porous material to better match the elastic modulus of human bone. However, titanium alloy is biologically inert and does not have antibacterial properties. At the same time, the porous structure with a large specific surface area also increases the risk of infection, leading to surgical failure. In this paper, we prepared three porous samples with different porosities of 60%, 75%, and 85%, respectively (for short, 3D-60, 3D-75, and 3D-85) using 3D printing technology and clarified the mechanical properties. Through tensile experiments, when the porosity was 60%, the compressive modulus was within the elastic modulus of human bone. Anodic oxidation technology carried out the surface modification of a 3D-printed porous titanium alloy with 60% porosity. Through change, the different voltages and times on the TiO2oxide layer on the 3D-printed porous titanium alloy are different, and it reveals the growth mechanism of the TiO2oxide layer on a 3D-printed unique titanium alloy. The surface hydrophilic and antibacterial properties of 3D-printed porous titanium alloy were significantly improved after modification by anodic oxidation.Keywords:Ti6Al4V porous titanium alloy;3D printing;anodic oxidation;antibacterial
研究领域:
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Research and Technology-JMR&T
·
2020
·
IF 5.289
Wei Wang,Yaping Liu,Gang Wang,Caiwang Tan,Wei Cao
In this study, the carbon fiber reinforced ZrB 2 -SiC composite ceramic was joined to Ti6Al4V alloy with Ag-Cu eutectic alloy filler at varied holding time and a fixed temperature of 800℃. Interface microstructure and shear strength of brazing joints were studied. The brazed ZSC f ceramics/AgCu/TC4 alloy is endowed with good metallurgical bondings. A typical interfacial structure of joints brazed for 10 min was found as ZSC f /TiC/Ti 5 Si 3 /Ag(s,s)/Cu(s,s) + TiC/TiCu/Ti 2 Cu/(TiC + TiCu)/TC4 alloy. Increase of holding time did not vary the precipitated phase of the joints but decreased thickness of the Ag(s, s) and Cu(s, s) reaction layer and gradually thickened the Ti-Cu reaction layer near the TC4 side. The formation process of reaction products was discussed in detail. The holding time substantially influenced shear strength of the joints. A maximum shear strength of 39 MPa was reached at a brazing time of 20 min.
研究领域:
材料科学
·
焊接工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）