BIOCHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 3.8
Meng Xianghe,Wang Shuyi,Lu Yuanchao,Nie Xiaohua,Ye Qin
Sciadonic acid (△5,11,14–20:3, SCA) is a rare versatile non-methylene-interrupted polyunsaturated fatty acid. To prepare high purity SCA from Torreya oils with 8.04 % purity, Ag + -assisted high-speed countercurrent chromatography (HSCCC) combined with urea complexation (UC) or argentation column chromatography (CC) was investigated. Results showed that n -hexane: acetonitrile (1:1, v/v) was suitable mobile phase for HSCCC, and SCA with purity of 23.74 % and recovery of 61.74 % (SCA purity>20 %) were obtained. When the Ag + addition was 0.50 %, one round of Ag + -HSCCC resulted in a recovery of SCA up to 95.38 % with purity around 21 %. It was believed that the enhanced performance originated from the reversible complexes between Ag + and C C in SCA through π-s * and d-π* bonds. Starting with low purity SCA of 8.04 %, HSCCC (23.74 %), UC (62.67 %), or Ag + -CC (60.41 % ) alone failed to produce SCA with high-purity (>90 %). However, Ag + -HSCCC combined with Ag + -UC or Ag + -CC yielded SCA with purities of 76.21 % and 97.74 %, and overall recovery of 21.38 % and 19.31 %, respectively. Thus, Ag + -HSCCC was an efficient method for the pre-enrichment of SCA, which could effectively facilitate coupling with UC, Ag + -CC or other PUFA purification techniques, resulted in significant improved purification performances.
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Journal of Integrative Plant Biology
·
2025
·
IF 9.3
Jiawen Yan,Zhihui Liu,Tongtong Wang,Ruoman Wang,Shuya Wang,Xiao Liu,Ya Liu,Jingwei Yan,Jiasheng Wu
Waterlogging stress is a major abiotic stress that severely limits plant growth and development. However, little is known about the effects of waterlogging stress on the growth and development of gymnosperms. In this study, we demonstrated that the TgRAV1–TgWRKY74–TgACS13 module regulates ethylene-enhanced root vitality during waterlogging stress in the gymnosperm Torreya grandis . Root vitality, the physiological status of root tissues, reflects their metabolic activity and cell viability of roots. Waterlogging stress induces ethylene accumulation in T. grandis roots, thereby enhancing root vitality. The ethylene biosynthesis gene TgACS13 positively regulates root vitality under waterlogging stress by increasing ethylene levels. The transcription factors TgWRKY74 and TgRAV1 directly regulate TgACS13 expression by binding to its promoter. Furthermore, waterlogging stress activates TgWRKY74 to promote TgACS13 transcription and alleviates the inhibitory effect of TgRAV1 on its expression, resulting in ethylene-enhanced root vitality during waterlogging stress. In addition, TgRAV1 interacts with TgWRKY74 both in vivo and in vitro , reducing the transcriptional activity of TgWRKY74 by inhibiting its DNA-binding ability without affecting the transcriptional activity of TgRAV1. Therefore, the TgRAV1–TgWRKY74 module finely tunes TgACS13 expression to regulate ethylene accumulation through multiple mechanisms, thereby maintaining the vitality of T. grandis roots exposed to waterlogging stress.
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PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Rabia Durrani,Hou Bowen,Erwann Durand,André Delavault,Hammad Ullah,Zeyuan Ling,Gao Fei,Song Lili
Five deep eutectic solvents were used to extract T. grandis nut protein, with water as comparison. ChCl:Urea was the most effective, preserving integrity and functionality of extracted proteins. Cold ethanol purification yielded highest protein recovery rate 6.89 % ± 0.12 % . Time, temperature, and solid-liquid ratio, were optimized using response surface methodology and a Box-Behnken design. Extracted proteins were characterized via SDS-PAGE to assess digestibility and functional properties. Proteins extracted by DES were further analyzed via LC-MS/MS. De novo sequencing identified 600 peptides with 235 proteins in DES extract, compared to 349 peptides and 143 proteins in the water extract. Furthermore, 87 functional oligopeptides in DES, compared to 41 in water. DES extract contain 63 antioxidant peptides, with three distinct peptides GGE, CME, and YIY. CME peptide had highest antioxidant activity. These findings highlighted DES for extracting smaller functional proteins and peptides from T. grandis , demonstrating potential in food industry applications for protein extraction.
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PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Song Shiqing,Wu Yuying,Wang Huatian,Xie Kun,Liu Hao,Sun Min,Shao Li,Yue Heng,Feng Tao,Yao Lingyun
The Torreya grandis meal (TGM) is a protein-rich by-product during the seed oil manufacturing process. In this study, the T. grandis meal protein (TGMP) was extracted from the defatted TGM by alkali extraction and isoelectric point precipitation, and the effect of in vitro digestion on peptide release of the TGMP was evaluated using static digestion model. The TGMP was composed of 17 amino acids with the molecular weight was major located at 35 kDa by SDS-PAGE. Under simulated digestion, the variation in peptide content, amino acid composition, and molecular weight distribution of the hydrolyzed TGMP were determined. The degree of hydrolysis (DH) of TGMP was revealed to be 16.52% after 120 min of gastric digestion and 18.85% after 270 min of gastrointestinal digestion, indicating that the simulated digestion is insufficient to hydrolyze the TGMP thoroughly. Meanwhile, the bioactivity assessment suggested that the antioxidant and anti-tyrosinase activity of the TGMP was significantly enhanced after simulated digestion. The obtained results would provide valuable information on the digestive characteristics of TGMP in the gastrointestinal tract, and would also help to develop functional peptides from the T. grandis meal.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Chengyu Jin,Siyuan Chen,Jue Ding,Qin Ye,Xianghe Meng
Sciadonic acid (SA, Δ5,11,14–20:3) is an unusual fatty acid found in Torreya grandis kernel oil with significant health benefits and functional value. However, obtaining high-purity SA from crude oil remains a challenge. In this study, we developed an efficient, eco-friendly, and selective method for SA enrichment using silver ion (Ag + )-loaded molecularly imprinted polymers (MIPs). Methacrylic acid (MAA) was selected as the functional monomer, with SA as the template molecule and AgNO 3 as the complexing agent. The polymerization was carried out using reversible addition-fragmentation chain transfer (RAFT)-modified Fe 3 O 4 @SiO 2 . Furthermore, adsorption studies revealed that the process followed the Langmuir isotherm model, while the adsorption kinetics were best described by the Pseudo-second-order model, with a maximum binding capacity of 97.04 mg/g. Then, Optimization experiments showed that the highest adsorption efficiency (84.62 %) was achieved using 20 mg of MIPs at 30 °C for 30 min. Desorption was most effective with n-hexane/acetic acid (9:1, v/v) for 8 h, yielding a desorption efficiency of 70.65 %. As a result, the final SA purity reached 93.11 %, with a recovery rate of 65.03 %. This novel Ag + -loaded MIPs strategy provides a rapid, selective, and environmentally friendly approach for SA enrichment, demonstrating the great potential for industrial application.
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Foods
·
2023
·Vol. 12
·期 21
·4005
·
IF 5.2
He Wang,Yue Zheng,Xinyue Tang,Ting Zhang
Loquat is a nutrient-rich fruit with juicy and sweet pulp, but it is vulnerable to rot and deterioration without proper postharvest preservation measures. This study aimed to improve the postharvest quality of loquat by developing a microemulsion system based on an essential oil extracted from theTorreya grandiscv. Merrillii aril (TaEO), which has antimicrobial and antioxidant properties. An optimal TaEO microemulsion (TaEO-ME) was formulated, using a mixture of Tween-40 and Tween-80 as the surfactant, 1-butanol as the co-surfactant, and TaEO as the oil phase, with mass ratios of 9:1, 3:1, and 6:1, respectively. Two TaEO-ME formulations with 60% and 70% water contents were stable for 180 days at room temperature, with a mean droplet size below 12 nm and polydispersity index less than 0.24. They also exhibited higher stability and enhanced biological activities compared to free TaEO. Loquat fruit treated with TaEO-ME displayed a reduced decay index and lower membrane lipid peroxidation after 15 days of storage at 15 °C, as indicated by the lower malondialdehyde content and higher peroxidase activity. Moreover, the TaEO-ME treatment preserved the nutrient quality by maintaining the total phenolic compounds and ascorbic acid content. Our findings suggested that TaEO-ME can be used as a substitute for chemical preservatives to keep fruits fresh.Keywords:microemulsion;Torreya grandiscv. Merrillii aril;essential oil;loquat;fruit quality
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Ningxiang Yu,Weixin Yao,Kaili Gao,Xiaohua Nie,Yuanchao Lu,Qin Ye,Shuai Hao,Xianghe Meng
High protein concentrations were often required for thermally induced gelation, posing challenges in cost and resource efficiency. This study investigates the effects and mechanisms of metal cations (Na + , K + , Mg 2+ , Ca 2+ , 0–0.4 mol/L) on the thermal gelation behavior of Torreya grandis nut protein (TGNP) with a low gelation concentration (3 %–6 %). However, the addition of metal cations induced structural unfolding and an increase in particle size of TGNP from 358 to 3205 nm, leading to a decrease in TGNP solubility by an average of 8 %, which reduced TGNP gel properties, including modulus, hardness, and water-holding capacity. Furthermore, the presence of metal cations decreased the native β-sheet and disulfide bond content and increased the surface hydrophobicity of the TGNP gels, resulting in a loose structure and poorer stability. Overall, this study provides novel insights for the formulation and application of TGNP in edible delivery gels such as plant-based milk.
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PubMed
高影响因子
Biomed Research International
·
2022
·Vol. 2022
·期 1
·5648896
·
IF 3.246
Xue-Qin Li,Shan-Shan Jia,Ke Yuan,Song-Heng Jin
Content . The water and ethanol extracts of Torreya grandis ( T. grandis ) have antioxidant, antibacterial, and hypolipidemic effects . Objective . The hypoglycemic effect and mechanism of the n-butanol fraction of the 75% ethanol extract of T. grandis leaves (BFTL) on rats with streptozotocin- and high-fat diet-induced type 2 diabetes mellitus (T2DM) were investigated. Materials and Methods . Sprague–Dawley rats were randomly divided into six groups: control, T2DM, metformin, high-dose BFTL (800 mg/kg), middle-dose BFTL (400 mg/kg), and low-dose BFTL (200 mg/kg). After 4 weeks of BFTL treatment, the correlations of serum indicators with protein expression in tissue were determined, and pathological changes in the liver, kidneys, and pancreas were analyzed. Results . Compared with the results in the T2DM group, serum fasting blood glucose, triglyceride, total cholesterol, malondialdehyde, alanine aminotransferase, and aspartate aminotransferase levels were significantly decreased ( p < 0.05), whereas superoxide dismutase and glutathione peroxidase levels were significantly increased ( p < 0.05) in the high-, middle-, and low-dose BFTL groups. The treatment also improved oral glucose tolerance. In addition, the pathological changes of the liver, kidney, and pancreas were improved by BFTL treatment. Cytochrome and caspase-3 expression in pancreatic was significantly decreased ( p < 0.05) by BFTL treatment, whereas the Bcl-2/Bax ratio was significantly increased ( p < 0.05). Discussion and Conclusion . BFTL exerted significant hypoglycemic effect on T2DM model rats, and its mechanism involved the suppression of blood glucose levels and oxidative stress by improving the metabolism of blood lipids and antioxidant capacity, boosting β -cell function, and inhibiting β -cell apoptosis.
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