JOURNAL OF ENTOMOLOGICAL SCIENCE
·
2024
·
IF 0.7
Gao Shanshan,Li Dongyu,Zhou Can,Zhu Min,Zhang Yonglei,Li Ruimin,Zhang Kunpeng
Evidence is accumulating that insect epsilon class glutathione S-transferases (GSTs) play an important role in the resistance to xenobiotics such as insecticides. A Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) epsilon class GST gene, TcGSTe17 , was previously found to be significantly induced following exposure to the plant derivative eucalyptol. However, whether TcGSTe17 is involved in resistance to eucalyptol remains unclear. In this study, TcGSTe17 was cloned from the T. castaneum . Development stage expression profiling revealed that TcGSTe17 was expressed at all development stages of T. castaneum but was highly expressed in late-stage larvae. Subsequent expression profiling of tissues showed that TcGSTe17 was highly expressed in the gut of larvae and the fat body of adults, and also revealed gender-specific expression patterns. In addition, the transcripts of TcGSTe17 were significantly increased following exposure to eucalyptol, and RNAi increased the susceptibility of the beetles to eucalyptol. Collectively, these results suggest that TcGSTe17 contributes to the susceptibility of T. castaneum to eucalyptol. These findings provide new data for the prevention of pest resistance.
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Journal of Essential Oil Bearing Plants
·
2023
·
IF 2.4
Wen Zhang,Xu-Dong Wang,Xiao-Han Zhang,Rui Feng,Jun-Long Wang,Wei-Bao Kong,Ji Zhang,Jun-Yu Liang
This study investigated the biological activity of essential oils (EOs) and their main components (caryophyllene and cuminol) against Tribolium castaneum larvae and Lasioderma serricorne adults. EOs were extracted from both flowers and leaves of Elsholtzia stauntoni. The results indicated that the EOs derived from both flowers and leaves, as well as their main components (caryophyllene and cuminol), exhibited contact activities with LD50 values of 35.11, 43.66, 15.44, and 26.05 μg/larva, respectively, against T. castaneum larvae. Moreover, they demonstrated obvious contact toxicities with LD50 values of 14.15, 11.18, 17.81, and 13.91 μg/adult, respectively, against L. serricorne adults. Additionally, EOs from both flowers and leaves exhibited fumigant activities against T. castaneum larvae (LC50 = 24.42 and 62.54 mg/L) and L. serricorne adults (LC50 = 15.26 and 24.29 mg/L). Furthermore, the EOs of flowers and leaves and their main components (caryophyllene and cuminol) remained significantly repellency to T. castaneum larvae and L. serricorne adults within the testing concentration range of 78.63-15.83 nL/cm2 after 12 h of exposure. These findings suggested that the EO from E. stauntoni and its two major components could potentially be developed as a natural insecticide for controlling T. castaneum larvae and L. serricorne adults.
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JOURNAL OF BIOLOGICAL CHEMISTRY
·
2024
·
IF 4
Huiling Zhou,Gaoke Lei,Yusi Li,Peng Chen,Zhiping Liu,Chengjun Li,Bin Li
Eclosion hormone (EH) is not only a key trigger of insect ecdysis, but is also involved in the regulation of important physiological processes such as development, diapause, metamorphosis and reproduction. EH is an ideal target for RNAi treatment and prevention of the Tribolium castaneum . However, two EH genes in Tribolium castaneum demonstrate distinct replication and functional conversion relationships, and the mechanisms of transcriptional regulation of eclosion hormone remain largely unexplored and poorly understood. In this study, the activity of highly active promoter fragments and potential transcription factors of TcEH and TcEHL were identified using the Dual-Luciferase reporter system and TANSFAC. TcSlbo and TcCAD were revealed to be important transcription factors for TcEH and TcEHL , respectively. Knockdown of TcSlbo failed to slough off the old epidermis of Tribolium castaneum and prevented them from developing into adults. Furthermore, we demonstrated for the first time that 20-hydroxyecdysone (20E) affects the expression of TcEH and TcEHL by regulating the transcriptional activities of TcSlbo and TcCAD. This study provides new insights into the transcription regulation of TcEH and TcEHL , their roles in insect growth and development, and the involvement of 20E in eclosion regulation, offering potential molecular targets for future pest management strategies.
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JOURNAL OF ECONOMIC ENTOMOLOGY
·
2021
·Vol. 114
·期 2
·979-987
·
IF 2.381
Cui Kaidi,He Leiming,Cui Guangrui,Zhang Tao,Chen Yue,Zhang Tao,Mu Wei,Liu Feng
Grain commodities in postharvest storage often deteriorate because of fungal and insect attacks. With the green consumption requirements of consumers, ecofriendly and safe pesticides are needed for grain storage. The current study investigated the efficacy of the plant volatile compound trans-2-hexenal against the storage insect pest Tribolium castaneum (Herbst) and three commonly occurring storage fungi, viz., Fusarium graminearum, Aspergillus flavus, and Aspergillus niger, to recommend its application as a botanical fumigant for grain commodities. trans-2-Hexenal weakly repels T. castaneum but has favorable insecticidal activity against multiple developmental stages of T. castaneum, ranging in sensitivity as follows: eggs (LC50 = 14.3 µl/l) > adults (31.6 µl/l) > young larvae (42.1 µl/l) > mature larvae (64.5 µl/l) > pupae (70.5 µl/l). Moreover, trans-2-hexenal caused a high malformation rate and high mortality in adults developed from fumigated pupae. In a 7-d grain, trans-2-hexenal at 0.8 µl/ml provided an appreciable efficacy (81.3%), and concentrations ≥ 0.1 µl/ml completely inhibited the offspring of T. castaneum. trans-2-Hexenal was nonphytotoxic to the seed germination and seedling growth of wheat seeds. Furthermore, trans-2-hexenal completely inhibited the growth of A. flavus, F. graminearum, and A. niger at 5, 10, and 10 µl/l, respectively. The favorable biological activity of trans-2-hexenal against T. castaneum and three frequently occurring mycotoxigenic storage fungi indicated the potential of trans-2-hexenal for simultaneously controlling pests and pathogens, which could reduce its application frequency in grains and decrease pesticide resistance risks.
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JOURNAL OF STORED PRODUCTS RESEARCH
·
2026
·
IF 2.8
Chunxue You,Yao Yao,Kaidi Dai,Jinming Chen,Yanming Wu,Wenjuan Zhang,Xiaoxue Yu,Chao Jiang,Xiumei Gao
Tribolium castaneum (Herbst) is a major stored-product pest, necessitating safer alternatives targeting its destructive larval stage. This study investigated the synergistic insecticidal effects of 2-undecanone and decanal, two active constituents from Houttuynia cordata essential oil, against T. castaneum larvae and elucidated the underlying mechanisms via quantitative real-time PCR (qRT-PCR), RNA interference (RNAi), histopathology, molecular docking, ELISA, and enzyme activity assays. Contact toxicity assays identified the 5:5 mixture as optimally effective, exhibiting significant synergy (R ≥ 1.5) and the lowest LD 50 (14.8 μg/larva). Whereas 2-undecanone alone upregulated farnesol dehydrogenase ( FDa ), glutathione S-transferase ( GST ), and cytochrome P450 ( CYP6BQ7 ), the 5:5 mixture significantly suppressed the expression of FDa and GST (>60 %), accompanied by decreased GST enzyme activity and lower juvenile hormone (JH) III levels. RNAi silencing of FDa and GST confirmed their important roles in larval susceptibility. Molecular docking predicted interactions of both compounds with FDa and GST, implying potential cooperative inhibition. Histopathology revealed more severe midgut damage under the mixture than under either compound alone. In conclusion, the synergy arises from coordinated suppression of the GST-mediated detoxification and FDa-linked JH biosynthesis, offering a promising strategy for developing potent, eco-friendly botanical formulations for stored-product pest management.
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JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2023
·
IF 1.5
Shanshan Gao,Xinyi Zhang,Can Zhou,Guangyan Zhang,Yonglei Zhang,Ruimin Li,Kunpeng Zhang
Insect odorant binding proteins (OBPs) paly an important role in the recognition and transmission signals. One odorant binding protein gene, TcasOBP7G , was significantly up-regulated after exposure to eucalyptol. Nevertheless, the causal role of TcasOBP7G in Tribolium castaneum is still unclear. Here, TcasOBP7G was cloned and characterized from T. castaneum , aligning with the typical characteristic of Minus-C OBPs. The analysis of gene expression profile showed that TcasOBP7G is highly expressed at late larval and adult stage, with particularly high expression levels in ovary and testis of adults, suggesting that TcasOBP7G might be associated with reproduction of T. castaneum. Furthermore, RNAi of TcasOBP7G led to a decrease in spawning, while the development of gonads was normal, suggesting that TcasOBP7G may medicate the reproduction of T. castaneum . Additionally, the mRNA level of TcasOBP7G was significantly increased after exposure to eucalyptol, and knockdown of TcasOBP7G resulted in an increased susceptibility of T. castaneum to eucalyptol, indicating that TcasOBP7G is associated with the tolerance of T. castaneum to eucalyptol. Altogether, these results indicate that TcasOBP7G is required for both reproduction and defense against phytochemical in T. castaneum . This study will provide a theoretical basis for the development of novel control strategies based on the olfaction of insects.
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JOURNAL OF STORED PRODUCTS RESEARCH
·
2024
·
IF 2.7
Junyu Liang,Xiaohan Zhang,Qian Zhang,Yufei Tang,Weibao Kong,Ji Zhang,Yue An
The main objectives of the present study are to introduce a ecologically safe, and natural compound for controlling the red flour beetle , Tribolium castaneum . This study aims to thoroughly examine the effects of Elsholtzia ciliata essential oil ( E. ciliata EO) and its primary components, carvone , on Tribolium castaneum eggs, larvae, pupae, and adults. The study also includes a simulated warehouse test to assess the efficacy of these substances as contact agents, fumigants , repellents, and antifeedants. All of these anti-insect activities were positively correlated with the concentrations of E. ciliata EO and carvone. Firstly, in the contact activity assay, E. ciliata EO and carvone completely inhibited the hatching of eggs and pupae of the T. castaneum at a concentration of 2.5 μL/cm 2 . The LC 50 of E. ciliata EO and carvone were 7.62 and 4.33 μg/adult, respectively, against senior adults (5 weeks old). Secondly, in the fumigant activity assay, 60 mg/L of E. ciliata EO and carvone essentially completely inhibited pupae eclosion . On the other hand, the LD 50 of E. ciliata EO and carvone against senior adults (5 weeks old) were 8.72 and 5.02 mg/L, respectively. Thirdly, for T. castaneum adults, at the highest test concentration, the repellent rate of the E. ciliata EO was above 90%. Fourthly, the larvae and adults of the T. castaneum could reach complete antifeedant activity by E. ciliata EO and carvone at the highest test concentrations. Finally, 3000 ppm of carvone was sufficient to kill all the pests in the warehouse in the simulated warehouse test. In conclusion, the results of this study will contribute to the better control of the storage pest , T. castaneum , and the development of environmentally friendly insecticides based on E. ciliata EO and carvone.
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Journal of Plant Diseases and Protection
·
2024
·
IF 2.1
Liang Jun-Yu,Yang Ying-Ying,Feng Rui,Zhang Xiao-Han,Wang Xu-Dong,Wang Jun-Long,Kong Wei-Bao,Zhang Ji
This study investigated chemical compositions and biocidal activities from the aerial parts of Rhododendron thymifolium against Tribolium castaneum , Lasioderma serricorne , and Ditylenchus destructor . In total, 13 components were isolated from the aerial parts of R. thymifolium by chromatographic separation techniques. Based on the results of MS, 1 H NMR, and 13 C NMR spectrometry, their structures were determined as ent-kaurane-16β-ol ( 1 ), pentacosane ( 2 ), undecanoic acid, ethyl ester ( 3 ), 1-Tridecanethiol ( 4 ), 1-Dodecanol ( 5 ), 4-Methyl-7-hydroxycoumarin ( 6 ), tertradecan-1-ol ( 7 ), apocynin ( 8 ), 3-Methoxy-4-methylbenzaldehyde ( 9 ), scopoletin ( 10 ), hyperin ( 11 ), quercetin ( 12 ), acetophenone ( 13 ). In addition, ent-kaurane-16β-ol ( 1 ) was isolated from R. thymifolium for the first time. Then, the insecticidal toxicities against T. castaneum , L. serricorne , and D. destructor . of compounds 1–13 were evaluated, and the results showed that apocynin ( 8 ) and acetophenone ( 13 ) exhibited obvious contact activity against the storage pests with LD 50 values of 6.46 and 8.72 μg/adult respectively. Acetophenone ( 13 ) showed excellent contact activity against D. destructor with LC 50 values of 0.08 mg/mL. The study clearly showed the active fractions from R. thymifolium have might potential to be developed into potential compounds on storage insect prevention.
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Insects
·
2024
·Vol. 16
·期 1
·31
·
IF 2.7
Shaohua Lu,Li Yang,Zonglin Wu,Mingshun Chen,Yujie Lu
Simple SummaryThe sense of smell helps insects perform essential behaviors like finding mates and food and avoiding dangers. Predators use unique smells to locate prey, but it is less understood if prey can recognize these predator odors and use them to escape. In this study, we examined howTribolium castaneum(Coleoptera: Tenebrionidae) andOryzaephilus surinamensis(Coleoptera: Silvanidae), two common pests, react to the predatorXylocoris flavipes, widely used in pest control. We found that these pests avoidX. flavipesodors, particularly the volatiles, linalool and geraniol, which decrease their attraction to food sources. These compounds show strong potential as natural repellents for pest management.The olfactory sensory system plays vital roles in daily activities, such as locating mate partners, foraging, and risk avoidance. Natural enemies can locate their prey through characteristic volatiles. However, little is known about whether prey can recognize the volatiles of their predators and if this recognition can increase the efficiency of prey escaping from predators.Xylocoris flavipesis a predator ofTribolium castaneum(Herbst) andOryzaephilus surinamensis(Linne) that has been widely used in stored pest control. Herein, we analyze the volatile components ofXylocoris flavipesand their impacts on the olfactory behavior ofT. castaneumandO. surinamensis. We found thatT. castaneumandO. surinamensispreferred blank air rather than odors ofX. flavipesandX. flavipesemissions, which significantly decreased the orientation preference ofT. castaneumandO. surinamensisto wheat.X. flavipesemits three major volatiles, including linalool, α-terpineol, and geraniol. Y-tube bioassays showed thatT. castaneumandO. surinamensiscan recognize linalool and geraniol at certain concentrations, especially at 200 μg/mL. EAG recordings verified that linalool and geraniol elicit higher olfactory responses in the two pests, but very small EAG responses were observed in the insects to α-terpineol. A further repellency evaluation also proved that linalool and geraniol are repellent to the two pests, and this repellency can be slightly enhanced by mixing them together.T. castaneumandO. surinamensiscan recognize the predatorX. flavipesby perceiving its volatiles and using them as signals for escaping. The two most potent volatiles, linalool and geraniol, may have potential values as repellents in controlling pests in these two stored products.
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PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2021
·Vol. 247
·期 10
·2609-2619
·
IF 2.998
Liang Junyu,Ning Anqi,Lu Peiyu,An Yue,Wang Zuoliang,Zhang Ji,He Chunyu,Wang Yongfeng
Stored-product pests are a major problem in food production and storage. Essential oils (EOs) have been reported to have a wide range of insecticidal activities. In this study, the EOs of flowers and leaves from Elsholtzia stauntoni were analyzed for their chemical composition by GC–MS. The two EOs and their two common main components (cuminol and caryophyllene) were evaluated for their bioactivities against T. castaneum adults. Results of GC–MS analysis indicated that the chemical composition of EOs of flowers and leaves from E. stauntoni had certain similarity and diversity. The two same major components of EOs of flowers and leaves were cuminol (27.81% and 27.62%, respectively) and caryophyllene (5.78% and 10.95%, respectively). The results of bioassays indicated that the EOs of flowers and leaves possessed obvious contact toxicities (LD 50 = 19.05 and 18.75 µg/Adult, respectively) and repellent activities against T. castaneum . In addition, the EOs of flowers (LC 50 = 16.26 mg/L Air) showed the higher fumigant toxicity than that of leaves (LC 50 = 44.42 mg/L Air). Cuminol and caryophyllene exhibited contact toxicity and repellency against T. castaneum , but showed no fumigant toxicity. In particular, cuminol was still significantly repellent to T. castaneum within the testing concentration range of 78.63–0.13 nL/cm 2 after 48 h. Furthermore, cuminol and caryophyllene exhibited synergistic contact activity against T. castaneum in all experimental ratios. Altogether, it suggests that EOs of E. stauntoni and their two major monomers have promising potential to serve as bio-insecticides for controlling T. castaneum .
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Foods
·
2020
·Vol. 9
·期 3
·345
·
IF 4.092
Jun-Yu Liang,Jie Xu,Ying-Ying Yang,Ya-Zhou Shao,Feng Zhou,Jun-Long Wang
Investigations have indicated that storage pests pose a great threat to global food security by damaging food crops and other food products derived from plants. Essential oils are proven to have significant effects on a large number of stored grain insects. This study evaluated the contact toxicity and fumigant activity of the essential oil extract from the aerial parts ofElsholtzia ciliataand its two major biochemical components against adults and larvae of the food storage pest beetleTribolium castaneum. Gas chromatography–mass spectrometry analysis revealed 16 different components derived from the essential oil ofE. ciliata, which included carvone (31.63%), limonene (22.05%), andα-caryophyllene (15.47%). Contact toxicity assay showed that the essential oil extract exhibited a microgram-level of killing activity againstT. castaneumadults (lethal dose 50 (LD50) = 7.79 μg/adult) and larvae (LD50= 24.87 μg/larva). Fumigant toxicity assay showed LD50of 11.61 mg/L air for adults and 8.73 mg/L air for larvae. Carvone and limonene also exhibited various levels of bioactivity. A binary mixture (2:6) of carvone and limonene displayed obvious contact toxicity againstT. castaneumadults (LD50= 10.84 μg/adult) and larvae (LD50= 30.62 μg/larva). Furthermore, carvone and limonene exhibited synergistic fumigant activity againstT. castaneumlarvae at a 1:7 ratio. Altogether, our results suggest thatE. ciliataessential oil and its two monomers have a potential application value to eliminateT. castaneum.Keywords:Elsholtzia ciliata;Tribolium castaneum;essential oil;carvone;limonene;insecticidal activity;synergistic effect
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高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2022
·
IF 1.58
Shan-shan Gao,Dong-yu Li,Zhuang-kun Huo,Yong-lei Zhang,Yi-zhuo Cao,Yue-yao Tan,Xin-long Guo,Jia-hao Zhang,Kun-peng Zhang,Rui-min Li
Insect glutathione S-transferases (GSTs) play a crucial role in the detoxification of exogenous compounds, especially insecticides and plant allelochemicals. A sigma class GST gene, TcGSTS7 , mediates the response to eugenol in Tribolium castaneum . However, the mechanism underlying this effect remains largely unknown. In this study, TcGSTS7 , which exhibits a structural motif and domain organization characteristic of GSTs, was cloned from the T. castaneum genome. Spatiotemporal expression analysis revealed that TcGSTS7 was most highly expressed at the late larva stage and was mainly expressed in the fat body and epidermis of larvae and adults, suggesting that TcGSTS7 may play a potential role in the protection against toxic xenobiotics in T. castaneum . Furthermore, the expression of TcGSTS7 was significantly induced after exposure to eugenol, while RNA interference (RNAi) targeting TcGSTS7 enhanced the sensitivity of the beetle to eugenol, indicating that TcGSTS7 is involved in the tolerance of T. castaneum to this insecticide. Interestingly, the depletion of TcCncC , which encodes a transcription factor of the CncC pathway that has been associated with the regulation of detoxification-related genes in insects, led to a reduction in the TcGSTS7 transcript level following exposure to eugenol, which suggests that TcGSTS7 acts downstream of the CncC pathway. Combined, these results indicated that TcGSTS7 participates in the tolerance of T. castaneum to phytochemicals in a CncC pathway-dependent manner. These findings have implications for the development of novel drugs for use in pest control.
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危险品化学品经营许可证（带存储）