Journal of Integrative Agriculture
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2023
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IF 4.8
Jia-nan SUN,Gao-yue SI,Hong-yi LIU,Ya-qian LI,Xin-hua WANG,Jie CHEN
Excessive use of organophosphate pesticides (OP), such as dichlorvos, in farming system poses a threat to human health through potential contamination of environment. To date, biodegradation has been prospected most promising approach to eliminate environmental OP residues. Trichoderma species as a biological control microorganism is often exposed to the chemical pesticides applied in environments, so it is necessary to understand the mechanism of degradation of dichlorvos by Trichoderma . In this study, dichlorvos significantly inhibited the growth, sporulation and pigmentation of T. atroviride T23, and the dichlorvos degradation activity of T23 required the initial induction effect of dichlorvos and the culture conditions, including the nutrient and pH values of the medium. Various changed primary and secondary metabolites released from T23 in the presence of dichlorvos were speculated as the energy and antioxidants for the strain itself to tolerate dichlorvos stress. The results showed that T23 could produce a series of enzymes, especially the intracellular enzymes, to degrade dichlorvos. The activities of the intracellular enzyme generated by T23 were differentially changed along time course and especially relied on initial dichlorvos concentration, ammonium sulfate and phosphate added in the medium. In conclusion, some dichlorvos-induced chemical degradation related enzymes of T23 were proved to be involved in the degradation of dichlorvos.
研究领域:
环境科学
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微生物学
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生物技术
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农药科学
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DOI
高影响因子
Biotechnology for Biofuels and Bioproducts
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2024
·Vol. 17
·期 1
·1-17
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IF 3.3
Liu Lin,Wang Zhixing,Fang Yu,Yang Renfei,Pu Yi,Meng Xiangfeng,Liu Weifeng
Background The saprophytic filamentous fungus Trichoderma reesei represents one of the most prolific cellulase producers. The bulk production of lignocellulolytic enzymes by T. reesei not only relies on the efficient transcription of cellulase genes but also their efficient secretion after being translated. However, little attention has been paid to the functional roles of the involved secretory pathway in the high-level production of cellulases in T. reesei. Rab GTPases are key regulators in coordinating various vesicle trafficking associated with the eukaryotic secretory pathway. Specifically, Rab7 is a representative GTPase regulating the transition of the early endosome to the late endosome followed by its fusion to the vacuole as well as homotypic vacuole fusion. Although crosstalk between the endosomal/vacuolar pathway and the secretion pathway has been reported, the functional role of Rab7 in cellulase production in T. reesei remains unknown.Results A TrRab7 was identified and characterized in T. reesei. TrRab7 was shown to play important roles in T. reesei vegetative growth and vacuole morphology. Whereas knock-down of Trrab7 significantly compromised the induced production of T. reesei cellulases, overexpression of the key transcriptional activator, Xyr1, restored the production of cellulases in the Trrab7 knock-down strain (Ptcu-rab7KD) on glucose, indicating that the observed defective cellulase biosynthesis results from the compromised cellulase gene transcription. Down-regulation of Trrab7 was also found to make T. reesei more sensitive to various stresses including carbon starvation. Interestingly, overexpression of Snf1, a serine/threonine protein kinase known as an energetic sensor, partially restored the cellulase production of Ptcu-rab7KD on Avicel, implicating that TrRab7 is involved in an energetic adaptation to carbon starvation which contributes to the successful cellulase gene expression when T. reesei is transferred from glucose to cellulose.Conclusion sTrRab7 was shown to play important roles in T. reesei development and a stress response to carbon starvation resulting from nutrient shift. This adaptation may allow T. reesei to successfully initiate the inducing process leading to efficient cellulase production. The present study provides useful insights into the functional involvement of the endosomal/vacuolar pathway in T. reesei development and hydrolytic enzyme production.
研究领域:
微生物学
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分子生物学
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酶工程
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DOI
高影响因子
CHEMISTRY OF NATURAL COMPOUNDS
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2021
·Vol. 57
·期 2
·312-314
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IF 0.809
Qin Chan,Hu Zhibo,Xiong Yahong,Chen Min,Li Chunyuan,Ding Weijia
A new sesquiterpene derivative, ethyl hydroheptelidate ( 1 ), was isolated from the mangrove endophytic fungus Trichoderma harzianum R1. Its structure was elucidated by comprehensive spectroscopic analyses. This compound showed high antifungal activity against two plant pathogenic fungi, Fusarium oxysporum and Colletotrichum musae , and moderate to weak antibacterial activity against three types of avian pathogenic Escherichia coli: O8, O78, and O101.
研究领域:
微生物学
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天然产物化学
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植物病理学
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2017
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IF 3.181
Jun Xia,Aiyong He,Rongqing Li,Yang Zhang,Jiaxing Xu,Xiaoyan Liu,Jiming Xu
Oxygen mass transfer is one of the most essential factors affecting microbial cell growth and cellulase fermentation. In this study, an effective approach capable of improving oxygen mass transfer was developed by adding oxygen vectors into the fermentation broth by Trichoderma reesei . With the addition of 1% (v/v) n -dodecane, the volumetric mass transfer coefficient ( k L a ) was increased from 142 h −1 to 185 h −1 at the beginning of the fermentation. In response to the improved oxygen transfer, 44.6% higher FPase activity, 29.2% higher xylanase activity and 42.5% higher saccharification yield of rice straw were obtained. The scanning electron microscopy showed that the addition of n -dodecane did not cause obvious variations on the cell morphology, whereas the comparative proteomics analysis identified 30 protein spots with different expression levels. Among them, exoglucanases (CBH I, CBH II), endoglucanase (EG VI), xylanases (Xyn I, Xyn II, Xyn III), acetyl xylan esterase, swollenin and cellulose induced protein (Cip1) were highly expressed after the addition of n -dodecane, whereas more EG III and β -xylosidase were produced without the addition of n -dodecane. The present study indicated that the addition of n -dodecane was advantageous for cellulase fermentation by T. reesei .
研究领域:
生物工程
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微生物学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Jing Li,Yumeng Chen,Ao Gao,Liujing Wei,Dongzhi Wei,Wei Wang
β-Carotene is an indispensable additive in beverage, cosmetic, feed, and pharmaceutical production. The fermentation industry annually generates abundant waste mycelia from Trichoderma reesei (T. reesei), a pivotal industrial strain for cellulase and heterologous protein production. In this study, we constructed a T. reesei cell factory for β-carotene production for the first time. Four key enzymes, CarRP, CarB, GGS1/CrtE, and HMG1, were overexpressed in T. reesei. The concentrations of medium components, including tryptone and glucose, were optimized. The modified strain accumulated β-carotene at a titer of 218.8 mg/L in flask culture. We achieved cellulase production (FPase, 22.33 IU/mL) with the concomitant production of β-carotene (286.63 mg/L) from T. reesei in a jar. Overall, this study offers a novel and unique approach to address the costly waste mycelium management process using T. reesei industrial strains that simultaneously produce proteins and carotenoids.
研究领域:
生物技术
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微生物学
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发酵工程
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代谢工程
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2019
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IF 5.839
Mengyun Jia,Jiajun Chen,Xiaozhen Liu,Mingyong Xie,Shaoping Nie,Yi Chen,Jianhua Xie,Qiang Yu
Trichoderma viride was utilized to ferment the defatted rice bran (DRB), and soluble dietary fiber was prepared before and after fermentation, named as B-SDF and F-SDF, respectively. Optimum fermentation conditions for yield of F-SDF were determined using response surface methodology (RSM). The yield of F-SDF was significantly increased from 10.5 (w/w, %) to 33.4 (w/w, %) under the optimal conditions (inoculum size = 10%, pH = 5.8 and time = 41 h). Fourier transform infrared spectroscopy (FTIR) demonstrated that both B-SDF and F-SDF had typical characteristics of the infrared spectrum of polysaccharides , and X-ray diffraction (XRD) revealed they were mainly substances of amorphous structure. In addition, F-SDF showed looser structure by scanning electron microscopy (SEM) analysis, increased molecular weight by high-performance gel permeation chromatography (HPGPC) analysis, and more complex monosaccharide composition by high-performance anion exchange chromatography (HPAEC) analysis. Furthermore, functional properties analysis showed that F-SDF exhibited higher water-holding capacity (WHC), oil-holding capacity (OHC), water solubility (WS) and cholesterol absorption capacity (CAC) than B-SDF. In summary, the present study suggested that SDF from defatted rice bran obtained through Trichoderma viride fermentation has a higher potential to be used as a functional ingredient in foods .
研究领域:
食品科学
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生物技术
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DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2020
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IF 3.644
Han Lijuan,Tan Yinshuang,Ma Wei,Niu Kangle,Hou Shaoli,Guo Wei,Liu Yucui,Fang Xu
In Trichoderma reesei, carbon catabolite repression (CCR) significantly downregulates the transcription of cellulolytic enzymes, which is usually mediated by the zinc finger protein Cre1. It was found that there is a conserved region at the C-terminus of Cre1/CreA in several cellulase-producing fungi that contains up to three continuous S/T phosphorylation sites. Here, S387, S388, T389, and T390 at the C-terminus of Cre1 in T. reesei were mutated to valine for mimicking an unphosphorylated state, thereby generating the transformants Tr_Cre1S387V, Tr_Cre1S388V, Tr_Cre1T389V, and Tr_Cre1T390V, respectively. Transcription of cel7a in Tr_ Cre1S388V was markedly higher than that of the parent strain when grown in glucose-containing media. Under these conditions, both filter paperase (FPase) and p-nitrophenyl-β-D-cellobioside (pNPCase) activities, as well as soluble proteins from Tr_Cre1S388V were significantly increased by up to 2- to 3-fold compared with that of other transformants and the parent strain. The results suggested that S388 is critical site of phosphorylation for triggering CCR at the terminus of Cre1. To our knowledge, this is the first report demonstrating an improvement of cellulase production in T. reesei under CCR by mimicking dephosphorylation at the C-terminus of Cre1. Taken together, we developed a precision engineering strategy based on the modification of phosphorylation sites of Cre1 transcription factor to enhance the production of cellulase in T. reesei under CCR.
研究领域:
分子生物学
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生物技术
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微生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2021
·
IF 9.381
Lei Ma,Zhiying Liu,Zhijian Kong,Mengmeng Wang,Tuo Li,Han Zhu,Qun Wan,Dongyang Liu,Qirong Shen
Lytic polysaccharide monooxygenases (LPMOs) are attracting much attention for their potential application in biodegradation. However, there are limited studies on the characterization of the AA11 family. Here, a novel AA11 family protein, Tg AA11, from Trichoderma guizhouense NJAU 4742 was characterized, and the isothermal titration calorimetry (ITC) analysis results showed that it exhibited tight binding capacity for copper ions with a K d value of 4.83 ± 0.79 μM. The MALDI-TOF-MS analysis results indicated that Tg AA11 could act on β-chitin to form C1 oxidation products, and some deacetylated chitooligosaccharides. In addition, the degradation of α-chitin and β-chitin by a chitinolytic enzyme Sg -chi was substantially increased in the presence of Tg AA11 by 39.9 % and 288.2 %, respectively. Furthermore, the active site residues predicted showed that His61 and Tyr142 might be critical for the active site residues of the Tg AA11 protein. This study will contribute to the understanding of the function of AA11 LPMOs in the degradation of chitin.
研究领域:
微生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
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2023
·
IF 6
Tao Zheng,Yong-Lin Ma,Wei-Sheng Li,Jian-Xin Deng,Han Li,Ming-Lei Luo,Tie-Wei Wang,Yan-Hui Wang
Trichoderma spp. have the potential to act as biocontrol agents for regulating plant growth. In this study, Trichoderma spp. were isolated from the rhizosphere soil of Oxalis corymbosa in sugarcane fields, and six strains were identified as T. koningiopsis , T. afroharzianum , T. atroviride , T. virens and T. asperelloides through morphology and phylogenetic analysis of ITS and tef 1 gene sequences. The activity of volatile organic compounds (VOCs) emitted by Trichoderma spp. was determined using the double-plate chamber method, revealing that five strains significantly inhibited the seed germination and growth of Echinochloa colona . Subsequently, SMPE-GC–MS and Venn analyses unveiled the presence of five common VOCs, namely phenylethyl alcohol, 2,4,6-trichloroanisole, hexadecanoic acid ethyl ester , 10(E),12(Z)-conjugated linoleic acid , and linoleic acid ethyl ester, within Trichoderma spp.. Independently, five compounds were evaluated for their inhibitory effect on the shoot growth of E. colona . Additionally, the results demonstrated that linoleic acid ethyl ester exhibited the highest inhibitory activity, while hexadecanoic acid ethyl ester was the least effective. The inhibitory effect of 2,4,6-trichloroanisole increased with concentration. Hormone analysis results indicated that VOCs influenced the concentration of abscisic acid , resulting in the maintenance of dormancy in E. colona . This study provides compelling evidence for the potential use of Trichoderma as bioherbicides.
研究领域:
植物学
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微生物学
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2018
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IF 5.807
Jian Du,Xiu Zhang,Xuezhi Li,Jian Zhao,Guodong Liu,Baoyu Gao,Yinbo Qu
Commercial cellulase preparations for lignocellulose bioconversion are mainly produced by the fungus Trichoderma reesei . The maximum cellulose conversion of T. reesei cellulase mixture was 15%–20% higher than that of Penicillium oxalicum in the hydrolysis of corncob residue and Avicel. Nevertheless, both preparations hydrolyzed more than 92% of cellulose in NaOH-mercerized Avicel. When added to Avicel hydrolysis residue that was less reactive to P. oxalicum cellulases, cellobiohydrolase I (CBH I) from T. reesei resulted in a higher cellulose conversion than its homologous proteins from P. oxalicum and Aspergillus niger at the same protein loadings. Further domain exchange experiment attributed the high hydrolytic efficiency of T. reesei CBH I to its inter-domain linker and cellulose-binding domain. The results in part explained the superior performance of T. reesei cellulases on the degradation of native crystalline cellulose, and highlighted the important role of cellulose-binding region in determining the degree of hydrolysis by cellulases.
研究领域:
生物化学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-14
·
IF 6.352
Xia Yanwei,Wang Jingfan,Guo Chuanxu,Xu Huanhuan,Wang Wei,Yang Mingzhu,Shen Qirong,Zhang Ruifu,Miao Youzhi
Background Filamentous fungi are highly efficient at deconstructing plant biomass by secreting a variety of enzymes, but the complex enzymatic regulation underlying this process is not conserved and remains unclear.Results In this study, cellulases and xylanases could specifically respond to Avicel- and xylan-induction, respectively, in lignocellulose-degrading strain Trichoderma guizhouense NJAU4742, however, the differentially regulated cellulases and xylanases were both under the absolute control of the same TgXyr1-mediated pathway. Further analysis showed that Avicel could specifically induce cellulase expression, which supported the existence of an unknown specific regulator of cellulases in strain NJAU4742. The xylanase secretion is very complex, GH10 endoxylanases could only be induced by Avicel, while, other major xylanases were significantly induced by both Avicel and xylan. For GH10 xylanases, an unknown specific regulator was also deduced to exist. Meanwhile, the post-transcriptional inhibition was subsequently suggested to stop the Avicel-induced xylanases secretion, which explained the specifically high xylanase activities when induced by xylan in strain NJAU4742. Additionally, an economical strategy used by strain NJAU4742 was proposed to sense the environmental lignocellulose under the carbon starvation condition, that only slightly activating 4 lignocellulose-degrading genes before largely secreting all 33 TgXyr1-controlled lignocellulases if confirming the existence of lignocellulose components.Conclusion sThis study, aiming to explore the unknown mechanisms of plant biomass-degrading enzymes regulation through the combined omics analysis, will open directions for in-depth understanding the complex carbon utilization in filamentous fungi.
研究领域:
分子生物学
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微生物学
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生物技术
·
酶学
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产品关联
DOI
PubMed
高影响因子
Microbial Cell Factories
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2021
·Vol. 20
·期 1
·1-12
·
IF 5.328
Jia Hexue,Sun Wan,Li Xuezhi,Zhao Jian
Background Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 (Cip1) is one of the most abundant proteins in extracellular proteins of T. reesei. Reported literatures about Cip1 mainly focused on the regulation of Cip1 and its possible enzyme activities, but the effect of Cip1 on the enzymatic hydrolysis of lignocellulose and possible mechanism have not still been reported.Results In this study, Cip1 from T. reesei was cloned, expressed and purified, and its effects on enzymatic hydrolysis of several different pretreated lignocellulose were investigated. It was found that Cip1 could promote the enzymatic hydrolysis of pretreated lignocellulose, and the promoting effect was significantly better than that of bovine serum albumin (BSA). And especially for the lignocellulosic substrate with high lignin content such as liquid hot water pretreated corn stover and corncob residue, the promoting effect of Cip1 was even better than that of the commercial cellulase when adding equal amount protein. It was also showed that the metal ions Zn2+ and Cu2+ influenced the promoting effect on enzymatic hydrolysis. The Cip1 protein had no lyase activity, but it could destroy the crystal structure of cellulose and reduce the non-productive adsorption of cellulase on lignin, which partly interpreted the promoting effect of Cip1 on enzymatic hydrolysis of lignocellulose.Conclusion The Cip1 from T. reesei could significantly promote the enzymatic hydrolysis of pretreated lignocellulose, and the promotion of Cip1 was even higher than that of commercial cellulase in the enzymatic hydrolysis of the substrates with high lignin content. This study will help us to better optimize cellulase to improve its ability to degrade lignocellulose, thereby reducing the cost of enzymes required for enzymatic hydrolysis.
研究领域:
生物化学
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生物技术
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微生物学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）