Food & Function
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2019
·Vol. 10
·期 7
·3934-3941
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IF 3.241
Ziye Zhang,Hang Xiao,Xiaofeng Zhang,Peng Zhou
Shrimp tropomyosin (TM) is a glycoprotein allergen with high lysine content, however, there is little data on how deglycosylation and glycation modify the allergenicity of TM. In this work, the effects of deglycosylation (enzymatic and chemical) and glycation of TM by glucose on in vivo allergenicity and mast cell function were investigated using BALB/c mouse and RBL-2H3 mast cells. Compared with TM, after deglycosylation, the deglycosylated TMs (DGTMs) exacerbated the in vivo allergenicity and mast cell allergy response. As for the TM glycated by glucose (TM-G), glycation led to weaker allergy responses on mouse and mast cells, which might be due to glucose destroying the epitopes with glycation sites. Moreover, the glucose residues on TM-G functioned as both a resistance to prevent the accessibility of TM-G to the IgE on mast cell and masking epitopes. Therefore, deglycosylation of TM was not suitable for desensitizing TM-induced food allergy, while glycation of TM might be applied to desensitize TM allergenicity, which could provide new insight into food allergy desensitization for larger cohorts of shrimp-allergic patients.
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FOOD CHEMISTRY
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2018
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IF 4.946
Linglin Fu,Chong Wang,Jinbao Wang,Saiqiao Ni,Yanbo Wang
Tropomyosin (TM), the predominant shellfish allergen, has induced severe food allergy worldwide, but efficient prevention and cure measures are limited nowadays. In the present study, we introduced an approach to eliminate Penaeus chinensis TM by Maillard reaction with reducing sugars including ribose, galacto-oligosaccharide (GOS) and chitosan-oligosaccharide (COS), which reduced the allergenicity by up to 60%. We also evaluated the conformational changes of TM during reaction by far-UV circular dichroism, and found the reaction-induced alternation from α-helix to β-sheet. We further determined the effect of Maillard reaction on TM allergenic epitopes to investigate the detailed mechanisms, and revealed the structure preference of different sugars. Correlation analysis with experimental data revealed that α-helix mediated the allergenicity elimination by Maillard reaction. There results provided theoretical foundations for the improvement and application of Maillard reaction in allergenic food processing.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
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IF 2.8
Hongbei Liu,Qiumin Xu,Michael Adu-Frimpong,Yuchu Chen,Ran Li,Fei Xu,Xia Cao,Shanshan Tong
In order to enrich the selection of biological ligands, realize the miniaturization analysis, and broaden the application of monolith materials for active ingredients screening and separating, we sough to construct a lipid raft @capillary monolith microcolumn affinity chromatography model. Single factor experiments and various characterization methods, including scanning electron microscopy (SEM) and thermogravimetric analysis, were employed to investigate the polymerization of the monolith column under different material ratios to determine optimal preparation conditions. Subsequently, the lipid raft from U251 cells was integrated with the monolith materials based on epoxy-based covalent crosslinking principle and characterized through SEM and immunofluorescence methods. Afterwards, the retention of positive drug gefitinib, negative drug gemcitabine and four licorice standards solution on the prepared lipid raft monolith microcolumn was then detected via electrochemical detection. The results exhibited that there was no specific adsorption for any active compounds on the blank monolith materials. Significantly, the lipid raft monolith microcolumn packed with TrkA-target proteins could be successfully validated for positive drug gefitinib with a high affinity sorption efficiency of 51.2%. This work expands the range of the utilization of affinity chromatography carriers and the selection of biological ligands, providing a new idea for the screening of active ingredients.
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Journal of Food Measurement and Characterization
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2024
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IF 2.9
Cheng Jun-Hu,Zhang Xinxue,Ma Ji,Sun Da-Wen
Tropomyosin (TM), the major allergen in shellfish, is a common protein cause of allergenic diseases, hence a sensitive, efficient method for TM detection is urgently needed to protect allergy sufferers in food products. In this work, a novel fluorescent aptasensor for TM-sensitive detection based on DNA-templated silver nanoclusters (AgNCs) and catalytic hairpin assembly (CHA) was designed. With the rapid separation and enrichment of magnetic beads, the detection of TM was converted to the detection of cDNA (complementary DNA). Then the cDNA was utilized as the initiator to successfully trigger the CHA amplification technique, which ultimately formed double-stranded to induce high fluorescence signals by AgNCs. Specifically, the cDNA could trigger repeated cycles, which enabled signal amplification and enhanced aptasensor performance with an excellent linear relationship in a wide linear range from 0.1 to 50 μg/mL (R 2 = 0.9967) and a desirable low detection limit of 0.0179 μg/mL. It is worth noting that the method was successfully validated with high specificity and stability for detecting processed shrimp products, implying great potential in the field of allergen assay.
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FOOD CHEMISTRY
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2024
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IF 8.5
Meixi Chen,Jun-Hu Cheng,Da-Wen Sun
Graphene oxide (GO), renowned for its two-dimensional structure and exceptional fluorescence quenching capabilities, is a preferred choice for the construction of fluorescence biosensors. As the sensitivity demands for these sensors escalate, enhancing the fluorescence quenching performance of GO and reducing background fluorescence become paramount to optimize the sensor sensitivity. In this study, the use of cold plasma (CP) treatment with glucose solution as a reducing agent to refine GO into reduced graphene oxide (r-GO) with optimal fluorescence quenching abilities was explored. The efficacy of CP treatment was comprehensively analyzed, encompassing fluorescence quenching capacity, morphological alterations, and structural composition. The findings revealed that the ideal conditions for achieving r-GO with superior fluorescence quenching were achieved by treating a mixture of 0.5 mg/mL GO and 0.2 g/mL glucose with CP at 100 kV and 1 A for 10 min. Notably, the resulting r-GO demonstrated remarkable performance in the specific detection of tropomyosin in shrimp, achieving a detection limit of 0.0657 μg/mL, demonstrating its potential for highly sensitive biosensing applications.
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JOURNAL OF CHROMATOGRAPHY A
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2023
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IF 4.1
Hao Chi,Sheng Tian,Xiu Li,Yuchu Chen,Qiumin Xu,Qixiao Wang,Wenwan Shi,Michael Adu-Frimpong,Shanshan Tong
In order to improve the separation process of affinity chromatography that has silica as the main carrier material, we sought to construct Lipid [email protected] 4B affinity chromatography model. We extracted the lipid rafts from U251 cells with a descaler method and sucrose density gradient centrifugation. Afterwards, it was discovered via immunofluorescence that the lipid rafts contain a large amount of tropomyosin-related kinase A (TrkA) protein. Also, agarose powder in the lyophilised state was pretreated, before the lipid rafts were coupled to the agarose gel in a coupling buffer of alkaline pH. CNBr-Sepharose 4B affinity gel packing was characterised using UV spectrophotometric, immunofluorescence and scanning electron microscopic techniques, wherein and the results showed that the lipid rafts were successfully coupled to the agarose gels. Three compounds were used to verify the specific sorption of Sepharose 4B and CNBr-Sepharose 4B, which showed no specific sorption on the materials. Of note, the prepared Lipid [email protected] 4B agarose gels packed with TrkA-rich target proteins could be successfully validated for the active drug gefitinib with high affinity sorption efficiency and eluted with good recovery and reproducibility. This study broadens the range of affinity chromatography carrier materials and provides a reference for research in active drug screening.
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JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Sujia Jiao,Xincheng Chen,Zhenyun He,Long Wu,Xiaoxia Xie,Zhichang Sun,Sihang Zhang,Hongmei Cao,Bruce D. Hammock,Xing Liu
Tropomyosin (TM) is the primary allergenic protein responsible for crustacean food allergies, and thus sensitive and rapid methods are required for the screening of crustacean TM in food. In this study, using the phage-displayed shark nanobody (PSN) as a multifunctional biomaterial, we developed a colorimetric and surface-enhanced Raman scattering dual-mode lateral flow immunosensor (CM/SERS-LFI) for competitive detection of crustacean TM. The SERS tag Au MBA @AgNPs with the Raman signal molecule 4-mercaptobenzoic acid (4-MBA) was prepared and immobilized on the PSN to construct the immunoprobe Au MBA @Ag-PSN. The probe can identify free TM that competes with TM on the T-line, and the optimized CM/SERS-LFI enables quantitative analysis of TM using the probe with a limit of detection (LOD) of 0.0026 μg/mL (SERS mode) and 0.0057 μg/mL (colorimetric mode), respectively. Additionally, it can implement a qualitative analysis by the naked eye with a visual LOD of 0.01 μg/mL. The CM/SERS-LFI exhibited excellent performance in the tests of selectivity, accuracy, precision, and stability. Moreover, the method’s effectiveness in the analysis of real samples was confirmed by a commercial ELISA kit. Therefore, the developed CM/SERS-LFI was demonstrated to be a powerful and reliable tool for the rapid and sensitive detection of crustacean TM in food.
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Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Xiangfei Hu,Hui Wang,Qiannan Jiang,Pingwei Wen,Jie Cheng,Haibin Liu,Yueming Hu,Zongcai Tu
The mechanism of H 2 O 2 combined pulsed electric field (PEF) treatment stimulating oxidation and decreasing allergenicity of parvalbumin of grass carp and tropomyosin of king prawn was clarified. Compared to H 2 O 2 treatment alone, the IgG-binding capacity of parvalbumin and tropomyosin significantly decreased by 21.87 % and 81.18 % after H 2 O 2 combined PEF treatment, while the IgE binding capacity reduced by 63.42 % and 76.91 %. •OH caused by electrochemical reaction triggered oxidative degradation of protein and disrupted their allergenic epitopes, which resulted in the structural unfolding, hydrophobic quenching and prevented the recognition by related antibody. Molecular dynamics showed that H 2 O 2 combined PEF treatment promoted •OH interaction with surface residues than alone H 2 O 2 treatment and PEF treatment, where the H 2 O 2 treatment and H 2 O 2 combined PEF treatment caused significant structural alterations of tropomyosin, which greatly converted α-helix to random coil. Furthermore, density functional theory calculation revealed that hydrogen abstraction at C α rendered more stable intermediates where the spin density of the unpaired electron was lower, while alcohol formed by addition of •OH to the side chains was pretty stable but their phenyl and imidazolyl states were more preferred than the saturated side-chains-containing ones.
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