JOURNAL OF CHEMICAL THERMODYNAMICS
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2022
·
IF 3.269
Lu Wang,YingLin Liu,Tianxin Weng,Xinyu Li,Yushu Wu,Yanna Zhao,Jie Liu,Min Liu
Bisdemethoxycurcumin (BDMC), one of the main curcuminoids, has numerous biological activities. However, the low solubility and stability of BDMC limit its application. In this work, in order to improve the solubility and stability of BDMC, we encapsulated BDMC using cetyltrimethylammonium bromide (CTAB) + Tween 20/Tween 60 mixed surfactants. The molar ratio of CTAB to Tween 20/Tween 60 was optimized by response surface analysis. The thermodynamic parameters (binding constant, enthalpy change, entropy change and Gibbs free energy change) for the interaction of BDMC with CTAB + Tween 20/Tween 60 mixed micelles were obtained by fluorescence spectroscopy and UV–visible absorption spectroscopy. Positive enthalpy change and entropy change indicated that hydrophobic interaction was the main driving force. Moreover, due to the longer alkyl chains of Tween 60, the encapsulation of BDMC by CTAB + Tween 60 mixed micelles was stronger. The encapsulation of BDMC in the hydrophobic alkyl chains by the mixed micelles was determined by fluorescence polarization technology, time-resolved fluorescence assay, and 1 H nuclear magnetic resonance spectroscopy. Solubilization and stability experiments showed that the solubility of BDMC was improved and the alkaline hydrolysis of BDMC was inhibited due to the encapsulation of the mixed micelles. The improvement of the solubility and stability of BDMC was more obvious in CTAB + Tween 60 mixed micelles because of the stronger interaction between them. The above results indicated that CTAB + Tween 20/Tween 60 mixed micelles could be used to encapsulate BDMC to improve its solubility and stability.
研究领域:
药学
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物理化学
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材料科学
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热力学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2020
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IF 3.99
Jiaxin Chen,Yangyang Feng,Baohua Kong,Xiufang Xia,Qian Liu
The present study established an eco-friendly extraction method for adsorbed proteins from the interfacial layers of oil-in-water (O/W) emulsions which stabilized by whey protein isolate. Tween 20, a typical food-grade low molecular weight surfactant , could sufficiently extract adsorbed proteins. Then, ultra-filtered centrifugation was applied to purify the adsorbed proteins. The results showed that the concentration of Tween 20 solution was a crucial factor impacting extraction efficiency, and concentration of Tween 20 from 0.5 % (v/v) could efficiently extract the adsorbed proteins from the oil-water interface. Moreover, when compared with the traditional isopropyl alcohol precipitation method, our developed method could efficiently extract, as well as maintain the unaltered structure of adsorbed proteins from different emulsions prepared with different concentrations of protein solution and different homogenization methods. Our method could substantially decrease the use of organic solvent within the laboratory setting, thereby minimizing the potential risks to the environment and human health.
研究领域:
食品科学
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环境科学
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蛋白质化学
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DOI
高影响因子
Food Biophysics
·
2022
·Vol. 17
·期 4
·545-556
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IF 3.34
Lv Danyu,Dai Chenming,Wang Muyun,Chen Huaiqiong,Guan Yongguang
Aqueous and photo stability of bixin are challenges in practical applications. Herein, transparent nanoemulsion can be self-emulsified to improve 9'- cis -bixin aqueous and photo stability. The 0.25 g carvacrol with 0.5% w/w 9'- cis -bixin, 2.08 g 12-% soybean lecithin, 2.25 g Tween 20, and 0.42 g water were mixed and stored overnight to fabricate transparent oil-in-water (O/W) nanoemulsion. The O/W nanoemulsion droplets had an average dimeter of 12.03 nm and equivalent spherical diameter of 6.78 nm. Photo stability of 9'- cis -bixin was improved after encapsulating in nanoemulsion droplets, demonstrated by significant improvement of 9'- cis -bixin degradation rate constant under ultraviolet. Nanoemulsion droplets significantly improved cell uptake of 9'- cis -bixin especially at the first 15 min after cocultivation of Caco-2 cells and 9'- cis -bixin encapsulated nanoemulsion. This study provides a comprehensive understanding of bixin protection by nanoemulsion fabricated by a self-emulsification method with potential cellular uptake.
研究领域:
药学
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纳米技术
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食品科学
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光化学
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2018
·Vol. 7
·期 22
·1800830
·
IF 5.609
Li Song,Xinghua Dong,Shuang Zhu,Chunfang Zhang,Wenyan Yin,Xiao Zhang,Xiangfeng Liu,Zhanjun Gu
Although various types of photothermal agents are developed for photothermal cancer therapy, relatively few photothermal agents exhibit high tumor inhibition rate under relatively mild conditions. Herein, a multifunctional Bi 2 S 3 –Tween 20 nanoplatform loaded with PI3K inhibitor LY294002 is designed as a novel photothermal agent for inhibitor and photothermal synergistic therapy of tumors under mild photothermal therapy conditions. The LY294002 of PI3K inhibitor, after being loaded by Bi 2 S 3 –Tween 20 nanodots, exhibits greatly increased drug utilization and reduced side effects on normal tissues. In vivo, Bi 2 S 3 –Tween 20@LY294002 upon near-infrared 808 nm laser irradiation shows potent antitumor activity under relatively mild conditions (power density: 0.6 W cm −2 ). Moreover, the mechanism studies also demonstrate that Bi 2 S 3 –Tween 20@LY294002 potently kills LoVo cancer cells under low-power near-infrared light irradiation, by downregulating the expression of heat shock protein 70 (HSP70) so as to increase the sensitivity of tumor cell hyperthermia and activating BAX/BAK-regulated mitochondrial apoptosis pathway. The results demonstrate that the newly synthesized multifunctional nanoplatform paves a new avenue for accurate therapy of photothermal-resistant cancer.
研究领域:
纳米技术
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癌症治疗
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药学科学
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生物医学工程
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2023
·
IF 10.7
Zhibin Chen,Wenbo Wang,Jie Xiao
The interfacial-bulk phase distribution behavior of antioxidants is closely related to their anti-oxidative efficiency. In this study, the dynamic distribution behavior of gallic acid (GA) in a zein particles-Tween 20 co-stabilized emulsions (CPE) and its impact on the oxidative stability of CPE were elucidated via a combined experimental and Dissipative Particle Dynamics (DPD) method. As the concentration of TW20 increased, the competitive displacement of Zp from the interface was observed. Consequently, the interfacial loading of Zp decreased, leading to a corresponding decrease in the content of GA on the interface. The study's pseudo-phase model provided quantitative data indicating that the interfacial partition percentage of GA decreased from 70.72% to 48.63%, while the interfacial adsorption rate of GA decreased from 0.039 to 0.015. A notable discovery was made in emulsions solely stabilized by Zp, where 17.58% of GA was lost at the interface through free diffusion. However, in CPE, this loss was significantly reduced to 9.12%. This reduction was achieved by maintaining an appropriate concentration of TW20 (ranging from 0.19 to 1.91 mM), which facilitated a higher interfacial distribution of GA in CPE. Additionally, inhibiting the free diffusion of antioxidants at the interface, as indicated by the diffusion coefficients ranging from 20.51 to 4.45, contributed to the improved oxidative stability of the CPE. The study not only provided insights into the phase-interface distribution behavior of antioxidants in emulsions but also offered in-situ determination methods to guide the interfacial engineering of emulsions.
研究领域:
食品科学
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化学工程
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产品关联
DOI
高影响因子
Micromachines
·
2023
·Vol. 14
·期 5
·902
·
IF 3.4
Yong Xie,Chao Bian,Mingjie Han,Ri Wang,Yang Li,Yuhao Xu,Shanhong Xia
A highly sensitive and accurate dual-signal strategy is developed for trace Cu2+detection based on the inner filter effect (IFE) between Tween 20-gold nanoparticles (AuNPs) and CdSe/ZnS quantum dots (QDs). Tween 20-AuNPs are utilized as colorimetric probes and excellent fluorescent absorbers. The fluorescence of CdSe/ZnS QDs can be quenched efficiently by Tween 20-AuNPs via IFE. In the presence of D-penicillamine, D-penicillamine induces the aggregation of Tween 20-AuNPs and the fluorescent recovery of CdSe/ZnS QDs at high ionic strength. Upon addition of Cu2+, D-penicillamine tends to selectively chelate with Cu2+and then forms the mixed-valence complexes, which consequently inhibits the aggregation of Tween 20-AuNPs and the fluorescent recovery. The dual-signal method is used to quantitatively detect trace Cu2+, with low detection limits of 0.57 μg/L and 0.36 μg/L for colorimetry and fluorescence, respectively. In addition, the proposed method using a portable spectrometer is applied to the detection of Cu2+in water. This sensitive, accurate and miniature sensing system has potential in environmental evaluations.Keywords:fluorescence;colorimetry;copper;CdSe/ZnS quantum dots;gold nanoparticles;inner filter effect
研究领域:
分析化学
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环境科学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AMB Express
·
2021
·Vol. 11
·期 1
·1-13
·
IF 3.298
Zhuang Wenjin,Liu Hanyu,Zhang Ying,He Junyao,Wang Pu
( R )-1-[3-(Trifluoromethyl)phenyl]ethanol (( R )-MTF-PEL) is an important chiral building block for the synthesis of a neuroprotective compound, ( R )-3-(1-(3-(trifluoromethyl)phenyl)ethoxy)azetidine-1-carboxamide. In this work, an effective whole-cell-catalyzed biotransformation was developed to produce ( R )-MTF-PEL, and its productivity was increased by medium engineering strategy. The recombinant E. coli BL21(DE3)-pET28a(+) - LXCAR-S154Y variant affording carbonyl reductase was adopted for the reduction of 3'-(trifluoromethyl)acetophenone to ( R )-MTF-PEL with enantiomeric excess (ee) > 99.9%. The addition of 0.6% Tween-20 (w/v) boosted the bioreduction, because the substrate concentration was increased by 4.0-fold than that in the neat buffer solution. The biocatalytic efficiency was further enhanced by introducing choline chloride: lysine (ChCl:Lys, molar ratio of 1:1) in the reaction medium, because the product yield reached 91.5% under 200 mM substrate concentration in the established Tween-20/ChCl:Lys-containing system, which is the highest ever reported for ( R )-MTF-PEL production. The optimal reduction conditions were as follows: 4% (w/v) ChCl:Lys, 12.6 g (DCW)/L recombinant E. coli cells, pH 7.0, 30 ℃ and 200 rpm, reaction for 18 h. The combined strategy of surfactant and NADES has great potential in the biocatalytic process and the synthesis of chiral alcohols.
研究领域:
生物化学
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合成生物学
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酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）