Food Analytical Methods
·
2016
·Vol. 10
·期 6
·2036-2045
·
IF 2.167
Zhang Bin,Li Huanhuan,Pan Wenxiu,Chen Quansheng,Ouyang Qin,Zhao Jiewen
Rare earth-doped upconversion nanoparticles (UCNPs) have promising potential in biodetection due to their peculiar frequency upconverting capabilities and high detection sensitivity. Here, we report a novel dual-color UCNP-based bacterium-sensing biosensor for simultaneously Escherichia coli and Staphylococcus aureus detection using UCNP as a fluorescence marker and conjugated with antibodies as the specific molecular recognition probe. Dual-color UCNPs were fabricated via varying lanthanide dopants to acquire the well-separated emission peaks. When E. coli and S. aureus were added into the reaction system, the recognition probes would capture the target bacteria through the specific binding of antibody. Then, the fluorescence intensities decreased (∆ I = I
o
-I ) were observed to increase linearly with the rising concentration of the E. coli (664 nm) and S. aureus (806 nm) from 47 to 47 × 10 6 cfu mL −1 ( R
2 = 0.98) and 64 to 64 × 10 6 cfu mL −1 ( R
2 = 0.97), respectively, resulting in the relatively low limit of 13 and 15 cfu mL −1 for E. coli and S. aureus . Furthermore, this UCNP-based bacterium-sensing biosensor could also be successfully applied to simultaneously detect E. coli and S. aureus in adulterated meat and milk samples.
研究领域:
纳米技术
·
生物医学工程
·
分析化学
·
食品安全
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DOI
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Ning Zhang,Xuelian Hu,Shuaijie Guo,Chang Lu,Siqi Zhang,Yiwei Tang,Tianyi Ma,Shuo Wang
Given its dual role in food quality-as both a spoilage indicator and regulated hazardous good, developing an accurate hydrogen sulfide (H 2 S) analysis is critical for food safety and public health. In this work, a novel triple-mode sensor based on fluorescence resonance energy transfer (FRET) between UCNPs@PEG and DMAMC was developed for H 2 S sensitive determination. The synthesized compound DMAMC enable rapidly capture H 2 S with good selectivity via nucleophilic addition, yielding brighter fluorescence, weaker visible absorption, and a paler purple hue. Under optimal conditions, the detection limits for the up-conversion fluorescence, visible adsorption, and colorimetric modes were 0.11 μM, 0.42 μM, and 0.60 μM, respectively. This platform achieved 97.10–107.40 % recoveries in complex matrices (tap water, milk, iceberg lettuce, and pork, RSD <6 %). The triple-mode sensor delivers a 3-min assay, robust anti-interference, and built-in self-calibration. Our work offers considerable potential to strengthen food-safety protocols.
研究领域:
分析化学
·
食品安全
·
纳米生物技术
·
光学传感
·
环境健康
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 16
·7206-7215
·
IF 7.394
Xiaoqian Ge,Liang Dong,Lining Sun,Zhengmei Song,Ruoyan Wei,Liyi Shi,Haige Chen
A new and facile method was used to transfer upconversion luminescent nanoparticles from hydrophobic to hydrophilic using polyhedral oligomeric silsesquioxane (POSS) linking on the surface of upconversion nanoparticles. In comparison with the unmodified upconversion nanoparticles, the POSS modified upconversion nanoplatforms [POSS-UCNPs(Er), POSS-UCNPs(Tm)] displayed good monodispersion in water and exhibited good water-solubility, while their particle size did not change substantially. Due to the low cytotoxicity and good biocompatibility as determined by methyl thiazolyl tetrazolium (MTT) assay and histology and hematology analysis, the POSS modified upconversion nanoplatforms were successfully applied to upconversion luminescence imaging of living cells in vitro and nude mouse in vivo (upon excitation at 980 nm). In addition, the doped Gd3+ ion endows the POSS-UCNPs with effective T1 signal enhancement and the POSS-UCNPs were successfully applied to in vivo magnetic resonance imaging (MRI) for a Kunming mouse, which makes them potential MRI positive-contrast agents. More importantly, the corner organic groups of POSS can be easily modified, resulting in kinds of POSS-UCNPs with many potential applications. Therefore, the method and results may provide more exciting opportunities for multimodal bioimaging and multifunctional applications.
研究领域:
生物医学工程
·
纳米医学
·
分子影像学
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Ruihan Tong,Huiming Lin,Yuhua Chen,Na An,Gang Wang,Xiaofan Pan,Fengyu Qu
To integrate photodynamic therapy (PDT) with chemotherapy for enhanced anticancer efficiency, near infrared (NIR) light mediated upconversion nanoparticles@mesoporous silica (UCNPs@mSiO 2 ) nanovehicle was constructed as the nanocarrier. Based on the novel ultraviolet (UV) emission, TiO 2 (3.0–3.2 eV) was adopted as the photosensitiver (PS) on account of the high activity and excellent stability. Here, a simple host-assembly method was exploited to graft it onto the inner and outer surface of the mSiO 2 shell. On the other hand, a UV cleavable o -nitrobenzyl derivative linker (TC linker) was prepared as “gate” to encapsulate anticancer agent doxorubicin (DOX) inside mSiO 2 , insuring the few prematurity. Under NIR irradiation, the UV emission can excite TiO 2 to produce reactive oxygen species (ROS) and also can induce the photodegradation of TC linker and drug release as well. The detailed release kinetics was studied to reveal that it is TC linker to manipulate the NIR-sensitive drug release and the amount of TC linker also can be used to adjust the release performance. The synergistic action of PDT and chemotherapy displays the improved cytotoxicity of DOX-UCNPs@mSiO 2 /TiO 2 -TC to HeLa cells under NIR irradiation that makes the potential application in anticancer field due to the superior to any single means.
研究领域:
肿瘤学
·
纳米医学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 7
·e4829
·
IF 3.2
Juanjuan Cheng,Xinru Wei,Lun Wang,Hongqi Chen
A ratio luminescence probe was developed for detecting Staphylococcus aureus ( S. aureus ) based on luminescence energy transfer (LET) using double-wavelength emission (550 nm and 812 nm) upconversion nanoparticles (UCNPs) as donor, gold nanoparticles (AuNPs) as acceptor and the aptamer for S. aureus as the specific recognition and link unit. The LET process could cause luminescence quenching because of the spectral overlap between the acceptor and the donor at 550 nm. In the presence of S. aureus , S. aureus selectively combined with the aptamer, and the AuNPs left the surface of UCNPs, which weakened the quenching effect and restored the luminescence of UCNPs. Based on this, the ratio detection was realized by monitoring the change of the luminescence signal of the probe at 550 nm and taking the luminescence signal at 812 nm as the reference signal. Crucially, the probe has a fast reaction speed, with a reaction time of 25 min, and the detection of S. aureus is realized in the concentration range of 5.0 × 10 3 –3.0 × 10 5 CFU/ml, with the detection limit of 106 CFU/ml. Therefore, the ratio probe has great potential for detecting of S. aureus in food because of its high sensitivity, fast speed and good selectivity.
研究领域:
分析化学
·
生物医学工程
·
微生物学
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产品关联
DOI
PubMed
高影响因子
INTEGRATED FERROELECTRICS
·
2019
·
IF 0.486
Liping Zhou,Hao Hu,Yi-Xin Zhang,Qing-Ye Meng,Bing Yu,You-Qing Shen,Hai-Lin Cong
Dendrimers have become effective drug carriers and gene transfection vectors for medical biomaterials. In this experiment, UCNPs (NaGdF4:Tm, Yb@NaGdF4) and three-generation PAMAM dendrimers were prepared firstly; then NaGdF4:Tm, Yb@NaGdF4 was modified by Polyacrylic acid(PAA); PAMAM were grafted to the surface of the modified UCNPs by the reaction between amino groups and carboxyl groups; finally, O-nitrobenzaldehyde was grafted to the surface by Schiff base reaction.The synthesized upconversion materials were coated with PAA and PAMAM, then loaded with O-nitrobenzaldehyde to form spherical nanoparticles. The particle size of prepared nanoparticles is 75 ∼ 80 nm. The dendrimer complex can emit ultraviolet light to regulate the pH gradient of aqueous solution under the excitation of near-infrared light (980 nm), and can be used to kill cancer cells by changing the acidity of tissue cells; secondly, the cavity between dendrimers and dendrimers on the surface of the complex can be used in many fields such as drug delivery and gene transfection; the nanoparticles provide a new method for cancer treatment.
研究领域:
生物医学工程
·
纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Zhujun Ai,Huan Cai,Changjin Liu,Yan Zhao,Qing Fu,Ningke Fan,Yujian Li,Siqiao Li,Song Zhou,Chunyang Li,Juan Li,Shijia Ding,Rui Chen
Fluorescent-colorimetric nanoparticles (FCNPs) attract considerable interest as an emerging dual-signal reporter for on-site qualitative/quantitative point-of-care testing. However, the suboptimal signaling components and self-assembled structure lacking physical isolation in traditional FCNPs result in low fluorescence brightness, poor stability, and strong internal filtration effect (IFE), which severely limits their wide application in lateral flow assay (LFA). Here, ordered self-assembly for hydrophobic upconversion nanoparticles (UCNPs) is developed using 3D porous space magnetic dendritic mesoporous silica (MS), stepwise surface silanization, and polydopamine (PDA) flexible scaffold modification to fabricate MS@UCNPs@PDA (MSUD). With rational design, MSUD improves stability and luminescence intensity (131 times higher than quantum dot-based fluorophores), and also eliminates IFE and fluorescence background interference on LFA strips. The detection limits of MSUD-labeled LFA for qualitative and quantitative detection of methamphetamine by naked eye-based colorimetric and smartphone-based fluorescence strategy are 1.047 × 10 4 pg mL −1 and 47.25 pg mL −1 , ≈10- and 2116- times lower than that of gold nanoparticles-LFA, respectively. The practicality of the MSUD-based LFA is validated in 83 urine/hair forensic samples, with the quantitative determination results in good agreement with the liquid chromatography-mass spectrometer data. This work presents an innovative strategy for constructing FCNPs, facilitating their progressive development and widespread applications.
研究领域:
分析化学
·
纳米医学
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2026
·
IF 8.2
Wenqing Chen,Xiaohui Lin,Tianmei Zhang,Yu Luo,Zhimei Huang,Danlei Chen,Xingwu Zheng,Jian Wen
This upconversion nanoprobe shifts Er 3+ emission from 540 to 660 nm for efficient Ce6 excitation. • First report that Ce6-loaded upconversion nanoparticles are excellent biocompatible and target ESCC cells. • The UCNPs@Ce6 platform dually functions: with PDT, ROS‑mitophagy/apoptosis alone, autophagy‑mediated anti‑inflammation. Chlorin e6 (Ce6), an efficient photosensitizer, suffers from poor biocompatibility, and lanthanide-doped upconversion nanoparticles (UCNPs) possess unique optical properties, yet their roles in esophageal squamous cell carcinoma (ESCC) remain unclear. In this study, Ce6-loaded UCNPs (UCNPs@Ce6) with a core–shell-shell structure was designed and synthesized to overcome the limitation of conventional photodynamic therapy (PDT) that relies on shallow-penetrating light. In vitro, UCNPs@Ce6-PDT selectively inhibited ESCC cells without affecting normal esophageal epithelial cells. Mechanistically, PDT induced mitochondrial dysfunction, mitophagy, and apoptosis via the reactive oxygen species (ROS)-mitochondrial pathway, whereas UCNPs@Ce6 alone activated autophagy and inhibited pyroptosis/necroptosis-related inflammation. In vivo, the fluorescence image-guided tumor therapy achieved tumor growth inhibition (73.72% ± 2.88%) without obvious toxicity. Collectively, this nanoplatform provides a safe and effective strategy for targeted therapy of ESCC by combining PDT-triggered mitophagy-related apoptosis with autophagy-mediated anti-inflammatory responses. Download: Download high-res image (151KB) Download: Download full-size image
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Lina Zhu,Yixin Zhang,Linhui Xu,Mingming Zhang,Qin Ouyang
Acetamiprid pesticide residues in food have raised increasing concerns in recent years. In this study, we developed a hydrogel fluorescent biosensor integrated with graphene oxide (GO) and upconversion nanoparticles (UCNPs) for acetamiprid detection. The biosensor was constructed by embedding GO and UCNP-aptamers conjugate into a hydrogel, forming a functional platform that combines structural robustness with optical responsiveness. Upon the presence of acetamiprid, it rapidly bound to the UCNP-aptamer conjugate, disrupting the fluorescence resonance energy transfer (FRET) effect between GO and UCNPs, leading to fluorescence recovery. The hydrogel exhibited excellent elasticity, with a stretchability (1100%), tensile strength (0.039 MPa), elastic modulus (0.0074 MPa), and toughness (25.27 MJ/m 3 ). The biosensor achieved a low limit of detection (LOD) of 0.108 ng/mL, and its practical applicability for acetamiprid detection was confirmed in real tea samples, with results consistent with standard HPLC technique ( p > 0.05 ). These findings highlight the potential of this hydrogel-based platform for the quantitative and on-site detection of pesticide residues, offering promising prospects for enhancing food safety.
研究领域:
生物传感器
·
纳米技术
·
食品安全
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 27
·5883-5891
·
IF 5.344
Shuhan Wang,Wensong Xi,Zhuo Wang,Huijun Zhao,Lei Zhao,Jianhui Fang,Haifang Wang,Lining Sun
It remains a great challenge to integrate effective photothermal therapeutic materials with upconversion nanoparticles (UCNPs) into one structure with small size. Herein, a new and simple method was developed to combine the luminescent UCNPs with vanadium disulfide (VS2) heterogeneously growing on the UCNPs. VS2 was grown directly on the surface of UCNPs to obtain oil-soluble nanocomposites, UCNPs@VS2. Then polyethylene glycol (mPEG) was functionalized on the surface of the nanocomposites to improve the water solubility, resulting in the integrated nanostructure UCNPs@VS2-mPEG (with an approximate size of 25 nm) for bioimaging and photothermal therapy in vitro. Importantly, cytotoxicity test results show that the final nanostructure has good biocompatibility. Furthermore, due to the excellent photothermal effects of VS2 and the unique imaging function of UCNPs, the nanostructure shows effective photothermal therapy for HeLa cells and was successfully applied in magnetic resonance imaging and upconversion luminescence imaging in vitro. Therefore, this study demonstrates a simple yet powerful method of growing VS2 on the surface of UCNPs, which provides an effective method to establish one integrated nanostructure with a nanoscale advantage for dual-model bioimaging and treatment.
研究领域:
纳米医学
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Jiahe Hu,Jialan Shi,Yingqian Gao,Wei Yang,Ping Liu,Qinghao Liu,Fei He,Chunxu Wang,Tao Li,Rui Xie,Jiuxin Zhu,Piaoping Yang
Background It is important to explore effective treatment for liver cancer. Photodynamic therapy (PDT) is a novel technique to treat liver cancer, but its clinical application is obstructed by limited depth of visible light penetration into tissue. The near-infrared (NIR) photosensitizer is a potential solution to the limitations of PDT for deep tumor tissue treatment.Purpose We aimed to investigate 808 nm NIR light-excited UCNPs@mSiO2-Ce6-GPC3 nanocomposites for PDT in liver cancer.Methods In our study, 808 nm NIR light-excited upconversion nanoparticles (UCNPs) were simultaneously loaded with the photosensitizer chlorin e6 (Ce6) and the antibody glypican-3 (GPC3), which is overexpressed in hepatocellular carcinoma cells. The multitasking UCNPs@mSiO2-Ce6-GPC3 nanoparticles under 808 nm laser irradiation with enhanced depth of penetration would enable the effective targeting of PDT.Results We found that the UCNPs@mSiO2-Ce6-GPC3 nanoparticles had good biocompatibility, low toxicity, excellent cell imaging in HepG2 cancer cells and high anti-tumor effect in vitro and in vivo.Conclusion We believe that the utilization of 808 nm NIR excited UCNPs@mSiO2-Ce6-GPC3 nanoparticles for PDT is a safe and potential therapeutic option for liver cancer.
研究领域:
生物医学工程
·
癌症研究
·
纳米技术
·
治疗学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Pingyue Wang,Huanhuan Li,Md Mehedi Hassan,Zhiming Guo,Zheng-Zhu Zhang,Quansheng Chen
In this study, a highly sensitive upconversion fluorescence (FL) biosensor was developed for the detection of organophosphorus pesticides (OPs) based on an acetylcholinesterase (AChE) modulated FL “off–on–off” strategy. The luminescence of synthesized UCNPs could be quenched strongly by Cu2+ due to an energy transfer effect. Upon addition of AChE and acetylthiocholine (ATCh), the enzymatic hydrolysate (thiocholine) could seize Cu2+ from UCNPs-Cu2+ mixture, resulting in the quenched FL triggered on. OPs could irreversibly impede the activity of AChE, which caused the formation of thiocholine to decrease, thus, reduced the recovery of FL. Under the optimum conditions, a linear detection range from 0.1 to 50 ng/mL was achieved for the representative OPs (diazinon) with LOD of 0.05 ng/mL. Furthermore, the ability of the biosensor to detect OPs was also confirmed in adulterated environmental and agricultural samples. In validation analysis, the proposed sensor showed satisfactory results (p > 0.05) with GC–MS.
研究领域:
生物传感技术
·
食品安全检测
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）