Journal of Chemistry
·
2019
·
IF 1.727
Hongbin Guo,Mengqiao Chen,Mengran Li,Mingye Hu,Baohua Chen,Chengyan Zhou
Mangiferin (MG) is an active component in natural medicines, and various studies have been reported on pharmacological effects, but the low solubility and bioavailability of MG limit its wide application. The aim of the present study was to investigate the pharmacokinetic profiles of mangiferin (MG) and mangiferin monosodium salt (MG-Na) in rat plasma by UPLC-MS/MS, which were then compared between the two groups. An appropriate high sensitivity and selectivity ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was applied to the comparison of plasma pharmacokinetics in MG and MG-Na using carbamazepine as internal standard (IS). These results showed that there were statistically significant differences in the pharmacokinetic parameters between MG and MG-Na after a single oral administration at 100 mg/kg. When compared with pharmacokinetic parameters of MG, the AUC(0-t), AUC(0–∞), Cmax,K10, and Ka of MG-Na were increased by 5.6-, 5.7-, 20.8-, 8-, and 83.6-fold, while the Tmax and CL/F were decreased by 4- and 5.7-fold (), respectively. t1/2 value showed an increasing trend, but was statistically significant between the two groups. Moreover, the AUC value in the MG-Na group was significantly increased and the relative bioavailability was calculated to be 570% when compared with that of the MG group. These results suggested that the salification reaction of MG can effectively enhance gastrointestinal absorption and relative bioavailability by improving solubility and membrane permeability.
研究领域:
药理学
·
分析化学
✓ 已验证
DOI
高影响因子
Natural Product Communications
·
2024
·
IF 1.5
Meng S. Cheong,Lutfun Nahar,Satyajit D. Sarker,Hui Cao,Wai S. Cheang,Jianbo Xiao
Piceatannol is a stilbenoid, which has shown bioactivities in various cell culture models. However, its stability in cell culture medium is not clear. Here, UPLC-MS/MS was applied in situ to analyze the degradation products of piceatannol in Dulbecco's Modified Eagle's Medium (DMEM) and cell culture to investigate the compound's stability in DMEM. During the incubation with cell culture medium (at 4 and 37 °C), several piceatannol derivatives, such as an oxidation product (m/z 243.06), a reduction product (m/z 247.09), dimers (m/z 485.12 and 487.14) and trimers (m/z 727.18) were detected, which demonstrated the instability of piceatannol in cell culture conditions. To confirm if the new products during the incubation were generated due to the instability of piceatannol, ascorbic acid was added. The presence of ascorbic acid could significantly slow the degradation rate of piceatannol and the generation of piceatannol derivatives, which proved that the new products were generated by the degradation of piceatannol and indicated that the instability of piceatannol might be related to its antioxidant activity.
研究领域:
生物化学
·
药物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yueting Liao,Yaohai Zhang,Qiyang Zhao,Wei Xiang,Bining Jiao,Xuesu Su
Metal organic framework MIL-101(Cr) was successfully synthesized as a selective adsorbent by a facile self-assembly technique and combined with UPLC-MS/MS for enrichment and determination of neonicotinoids in fruit juices and tea beverages. Therein, MIL-101(Cr) was characterized by Fourier transform infrared spectrometry, scanning electron microscopy, X-ray diffraction, and N 2 adsorption–desorption isotherms. Box-Behnken design was applied to optimize the significant variables including the sorbent amount, pH of the matrix, eluant volume, extraction time and desorption time. The optimized conditions were sorbent amount of 20 mg, eluent volume of 1 mL, extraction time of 5 min and desorption time of 4 min. Under the optimized conditions, excellent sensitivity was achieved with the limit of detection of 0.0019–0.02 μg/L and limit of quantification of 0.0063–0.067 μg/L. Good linearity (R 2 ≥ 0.9988) was obtained within the range of 1–1000 μg/L, and the recovery was 71.04%-101.64% with relative standard deviations (RSDs) of 1.25%-8.82% in spiked samples. The inter- and intra-day RSDs were both<8.54%. The adsorption isotherms investigated for acetamiprid, thiacloprid, and imidacloprid pesticides better fitted with the Freundich adsorption isotherm with bimolecular adsorption capacity of 71.43, 68.49, and 50.38 mg/g, respectively. These results indicate that the nanocomposite is feasible for adsorption of the target analytes at trace amounts from fruit juices and tea beverages.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Yifu Jiang,Le Lang,Jingwen Zhang,Yining Ji,Chenglong Zhang,Ruiqin Yang,Xuwen Li
This study combined the structural characteristics of amanita peptide toxins to prepare a Fe 3 O 4 /P4VPBA/PPy/GO composite material as a magnetic solid-phase extraction adsorbent. The experimental results indicated that this adsorbent showed excellent selectivity for the extraction of amatoxins and phallotoxins from mushroom samples. Compared with traditional dispersive solid-phase extraction, this adsorbent enables rapid separation in the enrichment and elution recovery stages through the application of a magnetic field . Simultaneously, the study optimized the conditions for ultraperformance liquid chromatography–mass spectrometry (UPLC–MS/MS), ensuring both separation efficiency and accuracy while reducing the analysis time. The researchers established the MSPE–UPLC–MS/MS method for the determination of amanita peptide toxins in wild mushrooms. This method not only simplified and expedited the sample pretreatment process but also achieved qualitative and quantitative analyses in a short period, thus demonstrating high detection sensitivity and outstanding recovery rates. Furthermore, the developed method was successfully applied to analyze toxic mushrooms discovered in Nanjing, China. The potential for extensive utilization of this method is highlighted in the field of food analysis and detection.
研究领域:
分析化学
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食品科学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2024
·Vol. 39
·期 1
·e6055
·
IF 1.8
Jianhui Luo,Songshen Chen,WenYang Song,Yongtong Huang,Song Gao,Jiu Wang
Ethoxysanguinarine (ETSG), a benzophenanthridine alkaloid, exhibits diverse biological activities, including antibacterial, antifungal, anti-inflammatory, antioxidant, and anti-tumor effects. Despite these properties, limited research exists on ETSG in vivo pharmacokinetics due to its poor solubility and low bioavailability. In this study, we developed a rapid and specific UPLC-MS/MS method for ETSG bioanalysis. Sample preparation involved one-step protein precipitation using methanol and phellodendrine as an internal standard (IS). The Waters HSS T3 column (2.1 * 50 mm, 1.8 μM) employed a gradient elution with mobile phases A (2 mmol/L ammonium formate aqueous solution-formic acid [99.8:0.2, v/v]) and B (methanol-formic acid [99.8:0.2, v/v]). Mass analysis via Waters Q-mass spectrometer utilized positive scan mode and multiple reaction monitoring. ETSG and IS were detected at m/z 332.0 → 274.0 and 342.0 → 177.0, respectively, within 7.0 min. The method demonstrated excellent precision, accuracy, recovery, and stability, with a linear calibration curve (1.1–560 ng/mL) and strong correlation coefficient (0.9984). Successful pharmacokinetic evaluation in Sprague–Dawley rats included intravenous ETSG administration and intragastric ETSG nanoemulsion/suspension. This method enables steroidal saponin analysis from ETSG in biological samples.
研究领域:
药物分析
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生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Tao Zheng,Jia-qian Sun,Xiao-jun Shi,Du-ling Liu,Bing-yin Sun,Yuanjie Deng,Ding-ling Zhang,Shu-ming Liu
Through field investigation, UPLC-MS/MS technology and MaxEnt model were performed to predict the suitable distribution area for red huajiao ( Zanthoxylum bungeanum maxim.) in China from 2021 s to 2060 s, and evaluate the effects of climate factors on the quality of red huajiao. The results demonstrated that mean temperature of the coldest quarter and min temperature of the coldest month were the most important environmental variables influencing red huajiao distribution. Suitable habitats for red huajiao were located mainly in dry and hot valley zone in the Qinba Mountains and the semi-humid and semi-arid areas of the Loess Plateau. The amides contents were higher in high suitability areas, while it was decreased in medium and low suitability areas, and temperature, wind speed and precipitation played a key role in their accumulation. This investigation was of great significance for the planting area optimization, quality control, benefit improvement and industrial development of red huajiao.
研究领域:
植物学
·
生态学
·
药用植物研究
✓ 已验证
DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 41
·26704-26711
·
IF 4.036
Zhouhong Tan,Yunbo Wen,Kai Yang,Xia Zhao,Xiaoli Yang,Jieli He
The combined abuse of benzodiazepines and antipsychotics has become a global problem, and to develop a highly sensitive and selective method for monitoring of benzodiazepine hypnotics and antipsychotics is urgently necessary. In this work, we established a rapid method for the simultaneous determination of benzodiazepines (diazepam, alprazolam, triazolam, and estazolam) and antipsychotic drugs (clozapine, and chlorpromazine) based on ultra performance liquid chromatography-mass spectrometry (UPLC-MS). The accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), specificity, matrix effect and carry-over effect were verified in detail. The results of the recovery and repeat experiments proved that the proposed UPLC-MS method possessed very satisfactory accuracy and precision. The LOD and LOQ of the six psychoactive substances were as low as 0.001–0.005 and 0.005–0.01 μg L−1, respectively. The proposed method was employed to analyze urine samples which were pretreated with a protein precipitation process. The potential influences of precipitants on the analysis results were evaluated statistically, and 0.1% formic acid/acetonitrile/water was selected as the optimum precipitation agent. The detection of the targets was free from matrix and carryover effects.
研究领域:
分析化学
·
法医学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Yan Yang,Xin Li,Jian Lin,Rong Bao
Antibiotics and sedatives are used in freshwater fish culture and transportation, and residue in freshwater fish pose potential risks to human health. Therefore, a throughput method was developed to detect antibiotic and sedative residues in fish, simultaneously quantifying 68 antibiotics and 9 sedatives in freshwater fish using a modified QuEChERS extraction method and UPLC-MS/MS. Matrix-matched calibrations demonstrated good correlation coefficients (R 2 > 0.995), with a recovery range of 66.2–118.5%. The intra-day and inter-day relative standard deviation (RSD) were below 9.7% and 12.8%, respectively. The limits of detection (LOD) and quantification (LOQ) were 0.08–1.46 μg/kg and 0.25–4.86 μg/kg, respectively. 68.8% of analytes had weak matrix effects, and 13.0% had moderate matrix effects. In addition, diazepam and many types of antibiotics were detected in30 freshwater fish. The validation parameters were in agreement with the acceptable criteria of the Codex guidelines. The method was effective in analyzing antibiotic and sedative residues in freshwater fish.
研究领域:
食品安全
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 17
·3117
·
IF 3.06
Mengqi Zhang,Xia Ren,Shijun Yue,Qing Zhao,Changlun Shao,Changyun Wang
Acanthus ilicifolius herb (AIH), the dry plant ofAcanthus ilicifoliusL., has long been used as a folk medicine for treating acute and chronic hepatitis. Phenylethanoid glycosides (PhGs) are one family of the main components in AIH with hepatoprotective, antioxidant, and anti-inflammatory activities. In this study, the pharmacokinetics of AIH was investigated preliminarily by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS). A simultaneously quantitative determination method for four PhGs (acteoside, isoacteoside, martynoside, and crenatoside) in rat plasma was first established by UPLC-MS/MS. These four PhGs were separated with an ACQUITY UPLC BEH C18column (2.1 × 50 mm, 1.7 μm) by gradient elution (mobile phase: MeCN and 0.1% formic acid in water, 0.4 mL/min). The mass spectrometry detection was performed using negative electrospray ionization (ESI−) in multiple reaction monitoring (MRM) mode. By the established method, the preliminary pharmacokinetics of AIH was elucidated using the kinetic parameters of the four PhGs in rat plasma after intragastric administration of AIH ethanol extract. All four PhGs showed double peaks on concentration-time curves, approximately at 0.5 h and 6 h, respectively. Their elimination half-lives (t1/2) were different, ranging from 3.42 h to 8.99 h, although they shared similar molecular structures. This work may provide a basis for the elucidation of the pharmacokinetic characteristics of bioactive components from AIH.
研究领域:
药理学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Saisai Ling,Ying Jin,Cuiyao He,Lisha Fu,Yuhua Deng,Xiaohui Qi,Zhenglei Wang,Yiying Wu
Tacrolimus is a potent macrolide immunosuppressant widely used in pediatric liver transplant patients. However, its narrow therapeutic window and significant inter-individual pharmacokinetic differences result in a poor correlation between blood concentrations and administered doses. To support its individualized dose, a sensitive, fast and robust ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to measure tacrolimus concentrations in whole blood of pediatric liver transplant patients. The method employed acetonitrile for protein precipitation, and sample separation was achieved using an Acquity UPLC CSH C 18 column (2.1 × 50 mm, 1.7 μm) with gradient elution. The mobile phase consisted of ammonium solution–water (0.5:1000, v /v) and methanol. The method demonstrated good linearity within a concentration range of 0.20–50.00 ng/mL. The intra- and inter-day precisions of tacrolimus in whole blood were less than 13.5 %, with the accuracy ranged from −7.2 % to 13.0 %. Selectivity, carryover, matrix effects, recovery, dilution reliability, and stability all met the standards set by relevant guidelines. The established UPLC–MS/MS method was successfully applied to measure the concentration of tacrolimus in the whole blood of pediatric liver transplant patients and support its individualized dose. Under the rapid UPLC–MS/MS method, 25 (65.8 %) patients required a dose increase, 3 (7.9 %) patients needed a dose reduction, and 10 (26.3 %) patients maintained their original dosage without adjustment.
研究领域:
药理学
·
临床医学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Talanta Open
·
2025
·
IF 3.7
Xiao-Chen Huang,Shou-Lian Wei,Su Yao,Yu-Tong Lin,Jin-Kui Ma
This study developed a novel dummy molecularly imprinted polymer (DMIP) via emulsion polymerization for rapid, sensitive, and simultaneous detection of four fluoroquinolone (FQ) antibiotic residues in tilapia. The DMIP, coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), was thoroughly characterized for its structure and adsorption properties. Optimized parameters revealed a rough surface morphology with aggregated spherical structures (400-500 nm). Compared to a non-imprinted polymer (NIP), the DMIP exhibited faster adsorption kinetics and enhanced selectivity towards enrofloxacin and related FQ compounds. The DMIP-UPLC-MS/MS method achieved satisfactory recoveries (77-105%) for the four target FQs in tilapia, with intra-day and inter-day relative standard deviations of 3.6-9.2% and 4.0-11.7%, respectively. Method detection limits (LODs) ranged from 0.02-0.06 µg/kg, while quantification limits (LOQs) ranged from 0.06-0.2 µg/kg. This novel method demonstrates excellent potential for highly sensitive and selective monitoring of trace FQ residues in aquatic food products.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Tianxing Wang,Wei Liu,Ling Chen,Xiaoxi Li
Foodborne heterocyclic aromatic amines (HAAs) are potent mutagens and carcinogens, posing significant health risks. Existing enrichment methods for HAAs need better adsorption selectivity and capacity for daily exposure assessment. This study hypothesized that introducing carboxylic groups into magnetic covalent organic frameworks (m-COFs) would improve HAAs adsorption by providing additional binding sites. Hence, we prepared a novel magnetic adsorbent, termed as Fe 3 O 4 @DOPA-TpPa-(COOH) 2 capable of enhancing the HAAs detection through magnetic solid-phase extraction (MSPE) coupled with UPLC-MS. This sorbent demonstrated a large specific surface area (130.7 m 2 /g), high magnetic responsivity (21.05 emu/g), and robust stability, with an adsorption capacity (Qm[cal]: 81.82 mg/g) driven by electrostatic, LP − π/C-H − π interactions, and hydrogen bonding. Optimal MSPE conditions provided sensitive detection with a broad linear range (5–500 ng/mL), low limits of detection (0.01–7.01 ng/g), and excellent repeatability. Application to Cantonese mooncake samples showed satisfactory recoveries (62.12%–126.86%). This method offers a more accurate tool for detecting HAAs.
研究领域:
食品安全
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）