Biomaterials Science
·
2025
·
IF 5.7
Xue-Wu Chen,Jun Long,Quan Zhang,Linghong Huang,Xin-Yuan Sun
s: Background: Calcium oxalate (CaOx) crystal deposition and its resultant cellular oxidative damage and inflammation are important causes of renal stone formation. It is clinically important to conduct research on multifunctional anti-stone drugs targeting these predisposing factors. Methods: We modified natural Undaria pinnatifida polysaccharide (UPP0) by sulfation via the sulfur trioxide-pyridine method, resulting in four sulfated polysaccharides with varying sulfate group (–OSO3–) contents: 1.59% (UPP0), 6.03% (UPP1), 20.83% (UPP2), and 36.39% (UPP3), and compared their differences in the inhibition of crystalline formation, renal injury, and inflammation in the process of renal stone formation at chemical and cellular levels. Results: The UPPS were able to inhibit the nucleation, growth and aggregation of CaOx crystals in vitro. Among them, UPP3 with the maximum sulfate group content showed the greatest crystallization inhibition ability. The nucleation inhibition and aggregation inhibition of UPP3 at a concentration of 0.5 mg/mL were as high as 80.21% and 72.34%, respectively. The CaOx crystal size regulated by UPP3 was significantly reduced from 25.9 ± 2.8 μm to 5.9 ± 1.2 μm. Furthermore, UPPS were observed to up-regulate the expression of the antioxidant enzyme superoxide dismutase (SOD) in cells, reduce the levels of ROS and malonaldehyde (MDA), enhance lysosomal integrity, decrease intracellular Ca2+ levels, inhibit the decline in mitochondrial membrane potential, reduce the production of cellular inflammatory factors (TNF-α, MCP-1, IL-18, and IL-1β), and ultimately inhibit cell apoptosis. Conclusion: UPPS combine multiple biological functions of crystallization regulation, antioxidant and anti-inflammatory, and have important potential in the prevention of kidney stones. Sulfation modification can improve the biological activity of UPP0 and provide a reference for screening and optimization methods of stone drugs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 5
·1015
·
IF 7
Xue-Wu Chen,Yu-Yun Zheng,Jian-Ming Ouyang
The clinical manifestation of primary hyperoxaluria includes hyperoxaluria and recurrent urinary calculi. In this study, an oxidative damage model was constructed based on oxalate damage to the human renal proximal tubular epithelial cells (HK-2), and a comparative study was carried out on four different sulfated levels ofUndaria pinnatifidapolysaccharides (UPP0, UPP1, UPP2, and UPP3 with sulfate group [–OSO3−] contents of 1.59%, 6.03%, 20.83%, and 36.39%, respectively) on the repair of oxidatively damaged HK-2 cells. The results showed that after repair by UPPs, cell viability was enhanced, healing ability was improved, the intracellular superoxide dismutase level and mitochondrial membrane potential were increased, malondialdehyde, reactive oxygen species, and intracellular Ca2+levels were reduced, cellular autophagy was reduced; lysosomal integrity was improved, and cytoskeleton and cell morphology were restored. The ability of repaired cells to endocytose nano-calcium oxalate dihydrate crystals (nano−COD) was enhanced. The activity of UPPs was closely related to their –OSO3−content. A too high or too low –OSO3−content was not conducive to polysaccharide activity, and only UPP2 exhibited the best cell repair ability and strongest ability to promote the cell endocytosis of crystals. UPP2 may be used as a potential agent to inhibit CaOx crystal deposition caused by high oxalate concentration.Keywords:Undaria pinnatifidapolysaccharide;acidic groups;cell repair;crystal endocytosis;primary hyperoxaluria
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2016
·Vol. 7
·期 5
·2398-2408
·
IF 2.686
Xiaofei Li,Jian Li,Zhike Li,Ying Sang,Yunhui Niu,Qianying Zhang,Hong Ding,Shanye Yin
Despite major scientific advances in its prevention, treatment and care, hypertension remains a serious condition that might lead to long-term complications such as heart disease and stroke. The great majority of forms of hypertension eventually result from an increased vasomotor tone activity that is regulated by endothelial NOS (eNOS) in vascular endothelium. Here, we examined the effect of fucoidan on eNOS activation in human umbilical vein endothelial cells (HUVECs). We also examined the effects of functional components of Undaria pinnatifida fucoidan on blood pressure and vascular function in eNOS inhibition-induced hypertensive rats in vivo. Our results suggest that fucoidan increased nitric oxide production by activating eNOS and Akt phosphorylation, which could be impaired by Akt or eNOS inhibitors. In the hypertensive rat model, treatment of fucoidan resulted in potent and persistent reduction of high blood pressure (BP) even after drug withdrawal. Our results showed that the mechanisms might involve protection against vascular structure damage, enhanced endothelium-independent vascular function and inhibition of abnormal proliferation of smooth muscle cells, which are mediated by the Akt–eNOS signaling pathway. Moreover, fucoidan treatment reduced the vascular inflammation and oxidative stress control caused by iNOS expression. Together, these results support a putative role of fucoidan in hypertension prevention and treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jiaying Ren,Yujing Lu,Yu Liu,Xu Huang,Kailing Sun,Hang Qi
Carotenoids, recognized for their antioxidant and anti-aging properties, are commonly used in functional foods. To enhance the application of fucoxanthin (FX) in the food industry, this study employed the ion gel method for encapsulating FX and combined it with raw materials such as Undaria pinnatifida homogenate and apple pieces to create freeze-dried crunchy chunks. The study evaluated the effects of encapsulated-FX on the functional and structural characteristics of the Undaria pinnatifida and apple freeze-dried chunks over accelerated storage period under high temperature and humidity. Various analyses were conducted, including physicochemical properties, texture analysis, color evaluation, sensory assessment, and simulated digestion analysis. The results demonstrated that the FX-rich freeze-dried crunchy chunks exhibited favorable structural properties and appealing flavor. Notably, after the accelerated storage period, the encapsulated-FX maintained significant antioxidant activity, along with excellent thermal and light stability, indicating high storage stability. Additionally, the main ingredients, sodium alginate (SAA) and pectin (PE), significantly enhanced the stability of FX during in vitro digestion. This study provided a straightforward approach for producing freeze-dried snack foods rich in stabled-FX, contributing to the diversity of value-added algae products. Furthermore, it laid a theoretical foundation and reference for the future development of nutritional products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2025
·
IF 4
Biao Yu,Baojun Xu,Karsoon Tan,Saiyi Zhong,Kit-Leong Cheong
Undaria pinnatifida oligosaccharides (UPO) are recognized for their multifaceted bioactive properties. Nevertheless, further investigation is required to establish the protective effects of UPO in mitigating UC. In this study, the structural characteristics of UPO were elucidated as 1,4-linked α-L-fucopyranose units with minor 1,2-linked β- d -galactopyranose units through NMR spectroscopy. UPO treatment improved the structural integrity of the intestinal barrier in DSS-induced colitis mice. Additionally, UPO administration induced the polarization of macrophages towards the anti-inflammatory M2 phenotype, evidenced by the downregulation of M1 macrophage marker (CD68) and upregulation of M2 macrophage marker (CD163). UPO treatment, significant alterations in the composition and diversity of bacterial and fungal microbiota were observed compared to colitis mice. UPO boosted the concentrations of short-chain fatty acids in fecal samples. These findings indicate that UPO could alleviate DSS-induced colitis in mice, potentially offering insights into its application as a novel therapeutic option for colitis treatment.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Pingrui Jiang,Weiyun Zheng,Xiaona Sun,Guoping Jiang,Shuang Wu,Yuxin Xu,Shuang Song,Chunqing Ai
Undaria pinnatifida was shown to reduce serum lipids and fat accumulation and produce beneficial effect on type 2 diabetes, but its effect on intestinal micro-ecology remains unclear. This study showed that sulfated polysaccharides from U. pinnatifida (UPSP) reduced weight gain, fat accumulation and metabolic disorders in mice fed with high fat diet (HFD). UPSP not only alleviated HFD-induced microbiota dysbiosis indicated as increased abundances of some Bacteroidales members that had positive correlations with the improvement of physiological indexes, but also maintained gut barrier integrity and reduced metabolic endotoxemia. A dose-effect relationship was observed between the dose of UPSP and its effect on some physiological indexes, gut microbiota community and nutrient utilization. The in vitro result showed that the use of Bacteroides species within Bacteroidales on UPSP was species-dependent, and the dose of UPSP affected the growth properties of some Bacteroides species. It implied that UPSP can be considered as prebiotic agent to prevent gut dysbiosis and obesity-related diseases in obese individuals.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhao-Rong Li,Rui-Bo Jia,Xueyuan Cai,Donghui Luo,Chong Chen,Mouming Zhao
The current study was aimed to enhance the solubility, dispersibility and biotransformation efficacy of ellagic acid (EA) by preparing food-derived ellagic acid- Undaria pinnatifida polysaccharides solid dispersion (EA/UPP SD). The results demonstrated that the solubility of EA/UPP SD was improved from 0.014 mg/mL to 0.383 mg/mL, and the enhancement was related to converting to a more amorphous state and restraining its self-aggregation during the mechanochemical process. The structure of EA/UPP SDs was mostly maintained by hydrogen bonds and hydrophobic interactions between EA and UPP . Moreover, the result of in vitro anaerobic incubations showed the biotransformation process was improved with EA/UPP SD addition to substrate due to the advance of microbial accessibility in EA dispersion. Altogether, these results indicated that the EA/UPP SDs expanded the application of EA by increasing the solubility and dispersity, and provided a theoretical basis for bioconversion efficiency enhancement.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 5
·2581-2593
·
IF 6.317
Panpan Zhang,Jinhui Jia,Pingrui Jiang,Weiyun Zheng,Xiangfei Li,Shuang Song,Chunqing Ai
Brown seaweed is rich in polysaccharides including sulfated polysaccharides and alginate, both of which provide health benefits to the host but whose differences have not received sufficient attention. In this study, alginate from Undaria pinnatifida (UPA) and sulfated polysaccharides from U. pinnatifida (UPSP) were isolated, and their action was analyzed in high fat diet-fed mice. UPA and UPSP improved body composition, fat deposition in body tissues and organs, lipid abnormality and inflammatory response in mice, and compound polysaccharides from U. pinnatifida (UPP: UPA + UPSP) had a better effect on some physiological indexes, which could be attributed to the differences in the gut microbiota. Both UPSP and UPA modulated diet-induced microbiota dysbiosis, and UPP had better effect on changes in the gut microbiota, including an increase in Bacteroidales and reduction in both Clostridiales and Lactobacillales that had positive correlations with the improvement of the physiological status. The in vitro model of bacterial culture revealed that the use of Bacteroides on UPA and UPSP was species dependent, and UPP can better maintain the diversity and stability of the Bacteroidales community. This study indicated that polysaccharides from edible brown seaweed can benefit host health by improving the intestinal microecology, which can be helpful for the application of edible brown seaweed in health foods.
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Advances
·
2022
·
IF 0
Xue-Wu Chen,Xin-Yuan Sun,Gu-Hua Tang,Jian-Ming Ouyang
Objective The formation of kidney stone is closely related to cell injury and crystal adhesion. Method The sulfur trioxide–pyridine method was used to sulfate raw Undaria pinnatifida polysaccharide (UPP) with a molecular weight (Mw) of 8.33 kDa. Four polysaccharides with the sulfate group (–OSO 3 − ) contents of 1.59% (UPP0), 6.03% (UPP1), 20.83% (UPP2), and 36.39% (UPP3) were obtained. The antioxidant activity of the four UPPs, the difference in oxidative damage inflicted by nano-CaOx monohydrate (nano-COM) on human proximal tubular epithelial (HK-2) cells before and after protection by UPPs, and the inhibitory effect on nano-COM adhesion were explored. Results Structural characterization showed that sulfation was successful. As the –OSO 3 − content in the UPPs was increased, the antioxidant activity and capability of the UPPs to regulate the growth of calcium oxalate (CaOx) crystals gradually increased. The damage caused by nano-COM crystals to HK-2 cells under protection by UPPs was weakened. This effect enhanced cell viability, enabled the maintenance of good cell morphology, reduced reactive oxygen species (ROS) levels, and inhibited the decrease in mitochondrial membrane potential, as well as decreased the eversion of phosphatidylserine (PS) and the expression of the adhesion proteins osteopontin (OPN), heat shock protein (HSP 90), and Annexin A1 (ANXA1). The adhesion of nano-COM to HK-2 cells was inhibited under the protection by UPPs. Conclusion UPP3 with the highest content of –OSO 3 − presented the best antioxidant activity and crystal regulation ability, while UPP2 with the second highest –OSO 3 − content showed optimal cell protection ability and crystal adhesion inhibition ability. The biological activity of UPPs was regulated by Mw and –OSO 3 − content. UPP2 with moderate –OSO 3 − content may become a potential drug for preventing CaOx stones.
✓ 已验证
DOI
PubMed
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Sanying Hou,Hailong Hu,Qing Fu,Tiyang Xiao,Jin-Qi Xie,Siew-Hwa Chan,Mengjun He,Bin Miao,Lan Zhang
The use of biomass as a catalyst offers a cost-effective and efficient approach for various processes, including the activation of peroxymonosulfate (PMS) to degrade contaminants. In this study, we successfully obtained Fe and N co-doped graphene-like hierarchical porous structure carbon (Fe-N/BC) by using undaria pinnatifida (wakame) as the precursor. The Fe-N/BC catalyst was utilized to activate PMS and degrade tetracycline (TC). Characterization results demonstrated an even distribution of Fe and N on the surface of the porous structure carbon. Compared to BC@PMS and N/BC@PMS, the Fe-N/BC@PMS system exhibited excellent degradation performance, achieving an 83.9 % degradation of TC in just 5 min with an ultralow catalyst dosage (Ca = 0.05 g/L) and a high TC concentration (TC = 0.03 g/L). Furthermore, experiments on radicals quenching and electron paramagnetic resonance (EPR) analysis indicated that singlet oxygen ( 1 O 2 ), a non-radical species, was demonstrated as the primary reactive oxygen species (ROS). The degradation mechanical analysis suggested that zero-valent iron (Fe 0 ), pyridinic N, graphitic N, and Fe-N x in the Fe-N/BC were the reason for the high catalytic activity. Moreover, the degradation pathways of TC were suggested through the use of LC-MS. Additionally, the ECOSAR system was employed to assess the toxicity of the intermediate products. This study reveals the active site and its mechanism in Fe-N composite biocarbon catalysts, providing a new method for synthesizing algal biocarbon catalysts.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）