SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Rui Duan,Xiaolei Hao,Yanzeng Li,Hanyang Li
In this work, a liquid crystal (LC) biosensor based on whispering gallery mode (WGM) lasing is reported and demonstrated for real-time and high-sensitive detection of acetylcholinesterase (AChE) and its inhibitors. Benefit from the double amplification by the WGM resonance and the 5CB molecules, small changes in biological processes are significantly enhanced in WGM spectral responses. The spectral responses provide direct information about the molecular adsorption/desorption at the LC/aqueous solution interface and can be used as an indicator of the enzymatic reaction. The limit of detection achieved was as low as 0.1 pg/mL for fenobucarb and 1 pg/mL for dimethoate, which is considerably lower than the standard levels of pesticides specified for water quality standards. Results indicate that this versatile method holds prospects for application in real-time and high-sensitive monitoring of biochemical reactions and can serve as an alternative solution to compensate for missing capabilities in conventional POM observations.
研究领域:
生物传感器
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分析化学
·
环境科学
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DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Yameng Li,Peilin Wang,Huan Wang,Zhenrun Li,Zihui Liang,Qiang Ma
MiRNA-214 can regulate the expression of their downstream target genes after post-transcriptional and are involved in the biological processes of triple negative breast cancer (TNBC). In this work, the small-sized luminescent Nb 2 C nanosheet-based whispering gallery mode-enhanced electrochemiluminescence (ECL) strategy was successfully constructed to detect miRNA-214 in TNBC. Firstly, we have synthesized small-sized luminescent Nb 2 C nanosheets from Nb 2 AlC MXene. The Nb 2 C nanosheets not only exhibited more stable chemical properties and reduced the defects of the large sheet structures, but also possessed the quantum confinement effect with the discrete energy level. As a result, the prepared small-sized Nb 2 C nanosheets had unique luminescent and electrochemical properties. Furthermore, in order to improve the ECL performance of Nb 2 C nanosheets, SiO 2 microspheres were self-assembled on the electrode surface by gas-liquid interface method to form whispering gallery mode structure. Because the light was continuously reflected at the interface of the microcavity in the whispering gallery mode, the ECL signal of Nb 2 C luminescent nanosheets was amplified largely. Finally, the whispering gallery mode-based ECL sensing platform was established. The results showed that the biosensor had a good linear correlation between the ECL intensity and the logarithm of concentration of miRNA-214 in the range of 10 fM to 100 nM with a limit of detection of 2.5 fM. The actual detection of miRNA-214 content in clinical TNBC tissue samples was realized successfully.
研究领域:
材料科学
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分析化学
·
生物医学工程
·
癌症生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Materiomics
·
2023
·
IF 9.4
Baoyuan Xu,Shuo Yang,Xingwei Feng,Tongjin Zhang,Zhenhua Gao,Yong Sheng Zhao
Micro/nanoscale photonic barcodes hold great potential for broad applications in items tracking, multiplexed bioassays and anti-counterfeiting. The ever-increasing demand in advanced anti-counterfeiting applications calls for micro/nanoscale barcodes with accurate recognition, large encoding capacity and high security level. Here, we proposed a strategy to construct the dual-stimuli responsive photonic barcodes based on the perovskite quantum dots (PQDs) doped polymer whispering-gallery-mode (WGM) microcavities via swelling-deswelling method. Benefiting from the well-defined spherical microcavities, the photoluminescence (PL) spectra of as-prepared composites exhibit a series of sharp peaks characteristics resulting from the effective WGM modulation, which constitutes the fingerprint of a specific resonator and thus allows a definition of photonic barcodes. On this basis, we achieved responsive photonic barcodes based on the volatile polar-solvent-controlled luminescence in the microspheres benefitting from the space-confined microcavities and the ionic feature of the PQDs. Moreover, the light-controlled photonic barcodes have further been acquired through reversibly regulating the inactivation and activation of the energy transfer (ET) process between the PQDs and photochromic dyes. The well-established protocols of PQDs@WGM enable the development of distinct responsive barcodes with multi-responsive features, which will pave an avenue to new types of flexible WGM-based components for optical data recording and security labels.
研究领域:
材料科学
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光学
·
纳米技术
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光子学
·
安全技术
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Peiyao An,Mengxiao Li,Shanshan Zhang,Zhenqi Cao,Miao Yu,Tangcheng Huang,Duo Wang,Yue Li,Rensheng Shen,Xin Yang,Jun Ren,Zhenlin Wu
As the fluoroquinolone antibiotics, norfloxacin has the advantages of antibacterial properties and efficient inhibition of bacterial DNA replication. Because of its strong binding affinity toward DNA helicases, this compound is chemically stable and exhibits limited biodegradation in environmental matrices. Prolonged use or discharge can enrich antibiotic resistance genes among microbial communities and contribute to persistent contamination. Herein, a nematic liquid crystal (LC) microlaser based on an aptamer recognition was reported and explored its application of norfloxacin ultrasensitive detection in soil. The rapid, real-time and quantitative monitoring of norfloxacin was achieved by exploiting whispering gallery mode (WGM) resonances in LC microdroplets functionalized with a specific aptamer as the molecular recognition element. Through resonance simulation and size-dependent experiments, the feasibility of using LC microlaser as a WGM microresonator was demonstrated. The optimal concentration of CTAB and DNA aptamer was 1 μM and 100 nM, respectively, which could realize the configuration transition of LC microdroplets from radial to bipolar. The devised norfloxacin sensor showed high sensitivity with a detection limit of 0.3 ng/mL, which was an order of magnitude lower than that reported for polarized optical microscope (POM) methods. In addition, the successful detection of various concentrations of norfloxacin in soil samples underscored the practical utility in environmental monitoring. The aptamer-based WGM-LC microlaser provides a highly sensitive approach for norfloxacin detection. The laser recognition probe does not require fluorescent or chromogenic labels, which affords significant advantages for rapid, real-time, and quantitative biosensing. This platform is expected to be extensible to ultrasensitive detection of other drugs, proteins, or environmental pollutants, particularly suited for integration into microfluidic devices for on-site environmental monitoring and public health risk assessment.
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产品关联
DOI
高影响因子
ACS Applied Optical Materials
·
2025
·
IF 3.8
Jianwei Wang,Yeshuai Song,Yue Wang,Fusen Bo,Xinyu Dou,Xinghua Yang,Yu Zhang,Zhihai Liu,Yanzeng Li,Hanyang Li
Real-time, label-free, and highly sensitive biochemical sensing is essential for early clinical diagnostics. Many conventional assays, such as colorimetry, fluorescence, and electrochemistry, are often limited by low sensitivity, complex procedures, and slow response times. Here, we proposed a whispering gallery mode (WGM) biosensing system based on stearic-acid-functionalized liquid crystal (LC) microcavities for biochemical detection. Amphiphilic stearic acid functionalizes the LC microcavity interface, enabling the pH-responsive reorientation of LC molecules. This changes the refractive index profile within the microcavity, resulting in obvious shifts in the WGM lasing spectra. Dynamic variations in molecular orientation within the LC microcavity were monitored using polarized optical microscopy and spectral analysis. Furthermore, this system allows for the highly sensitive detection of alanine aminotransferase and aspartate aminotransferase. Both liver biomarkers were detected in the range of 0.03–0.10U/mL, exhibiting a linear relationship with their respective enzymatic reaction times. WGM spectral wavelength shifts recorded at defined time points (60, 90, and 120 s) enable rapid estimation of enzyme concentrations, allowing preliminary quantification. This approach enables simplified operation, short response time, and label-free detection for early liver injury diagnostics, which offers a potential biochemical sensor for evaluating drug-induced liver injury and monitoring point-of-care liver function.
研究领域:
生物光子学
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生物传感器与生物电子学
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临床诊断
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光学传感
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生物医学工程
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产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Rui Duan,Yanzeng Li,Bojian Shi,Hanyang Li,Jun Yang
In this study, we demonstrate a novel nematic liquid crystal (LC) 4-cyano-4′-pentylbiphenyl (5CB) microdroplet-based biosensor for real-time, quantitative and sensitive detection of urea by whispering gallery mode (WGM) lasing technology. The single stearic acid-doped 5CB microdroplet is taken as both optical resonator and sensing reactor. To the best of our knowledge, this work is the first report of urea detection with WGM lasing. The enzymatic reaction between urea and urease produces hydroxide ions. Deprotonation and self-assembly of stearic acid occur at the aqueous/LC interface with increased pH value, promoting the reorientation of LC molecules. The detectable shift of WGM lasing spectra related to the configuration transition of LC microdroplets can be used as an indicator of enzymatic reaction, and this allows detection of urea molecules by the sensor at a concentration of only 0.1 mM. The proposed sensor also demonstrated the ability to detect urea in real urine samples. Compared with conventional POM observations, monitoring and identification of lasing spectra from LC microdroplets as a versatile method allow for more quantitative and sensitive detection of analyte reactions and can be expected to replace POM imaging technology for current LC-based biosensor systems.
研究领域:
生物传感
·
光学检测
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 23
·7595-7602
·
IF 3.978
Rui Duan,Yanzeng Li,Yichen He,Yonggui Yuan,Hanyang Li
In this study, we demonstrate a quantitative and sensitive strategy for monitoring the lipase concentration using a liquid crystal (LC) microfiber biosensor based on the whispering-gallery mode (WGM). An LC 4-cyano-4′-pentylbiphenyl (5CB) microfiber was doped with glycerol trioleate (GT) and used as both an optical microcavity and a sensing element. The self-assembled monolayer of a surfactant was formed at the LC/aqueous solution interface by the enzymatic reaction between lipase and GT, which resulted in the reorientation of the 5CB molecules from a planar to a homeotropic configuration. Interestingly, the structural change of the 5CB microfiber can be sensitively captured by the WGMs and quantitatively characterized by the wavelength shift. With this method, lipase with concentrations as low as 0.01 μg mL−1 in aqueous solution can be detected within 200 seconds, which is lower than the optimal value based on LC materials reported so far. In addition, our biodetection system is highly selective for lipase. We believe that the proposed biosensor has high potential for the detection of biochemical molecules.
研究领域:
生物化学
·
光学传感
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Ye Kong,Zhe Zhao,Yunqi Wang,Shuo Yang,Gaoshan Huang,Yang Wang,Chang Liu,Chunyu You,Ji Tan,Chao Wang,Borui Xu,Jizhai Cui,Xuanyong Liu,Yongfeng Mei
Carbon dioxide (CO2) sensing using an optical technique is of great importance in the environment and industrial emission monitoring. However, limited by the poor specific adsorption of gas molecules as well as insufficient coupling efficiency, there is still a long way to go toward realizing a highly sensitive optical CO2 gas sensor. Herein, by combining the advantages of a whispering-gallery-mode microcavity and a metal–organic framework (MOF) film, a porous functional microcavity (PF-MC) was fabricated with the assistance of the atomic layer deposition technique and was applied to CO2 sensing. In this functional composite, the rolled-up microcavity provides the ability to tune the propagation of light waves and the electromagnetic coupling with the surroundings via an evanescent field, while the nanoporous MOF film contributes to the specific adsorption of CO2. The composite demonstrates a high sensitivity of 188 nm RIU–1 (7.4 pm/% with respect to the CO2 concentration) and a low detection limit of ∼5.85 × 10–5 RIU. Furthermore, the PF-MC exhibits great selectivity to CO2 and outstanding reproducibility, which is promising for the next-generation optical gas sensing devices.
研究领域:
材料科学
·
光学工程
·
环境监测
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Liang Fu,Qijing Lu,Xianlin Liu,Xiaogang Chen,Xiang Wu,Shusen Xie
Lead ions are deleterious pollutants that often reach drinking water, and can cause significant harm to humans (particularly children). An ultra-sensitive lead ion detection method using a whispering gallery mode (WGM) optofluidic microbubble resonator and the classic GR-5 DNAzyme is proposed in this paper. With the auxiliary piranha and Ploy- l -lysine solution, GR-5 DNAzyme was successfully modified on the inner wall of a microbubble. The mode field distribution of the microbubble was analysed, and the optofluidic sensor with thin wall exhibited a maximum bulk refractive index sensitivity of 265.2 nm/RIU. Lead ions at concentrations ranging from 0.1 pM to 100 pM were tested using the proposed WGM optofluidic sensor. The noise was decreased to 2.43 fM using the self-referenced differential method. Thus, a limit of approximately 15 fM was obtained for the detection of lead ions using the WGM optofluidic biosensor. Eight competing metal ions were also used to evaluate the selectivity of the proposed sensor, with results indicating that it has high selectivity for lead ions. Finally, the sensor performance is verified using real samples.
研究领域:
光学
·
传感器
·
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Zhenyu Ma,Jing Sun,Xiaolan Li,Wenjun Shan,Yunxiang Yan,Yongjun Liu
Trypsin, a protease produced in the pancreas, plays a crucial role in various medical conditions, including acute pancreatitis, cystic fibrosis, and malnutrition, where abnormal trypsin levels are often implicated. Consequently, trypsin detection has emerged as a valuable diagnostic tool in clinical medicine. In this study, we introduced an integrated fiber sensor that capitalizes on the amplification and transduction capabilities of liquid crystals, coupled with the high sensitivity of the whispering gallery mode, to achieve precise trypsin detection. This innovative approach offers consecutive measurement and rapid response times , typically under 200 s, and remarkably low sample consumption, as little as 3 nL, potential for detection in capillary blood sampling. Furthermore, when subjected to validation tests for trypsin detection in human serum, our method demonstrated excellent consistency and reliability, with recovery ratios falling within the range of 97–112%. Beyond trypsin, this method's adaptability and versatility make it a universal technique with potential applications in the field of biosensing and biochemistry.
研究领域:
生物传感
·
医学诊断
·
光学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yan Wang,Ke-Xin Ma,Yu-Hao Hu,Bin Yin,Jin-Lei Wu,Rui Duan,Yan-Yang Zhou,Hai-Yan Wang,Li-Ying Jiang
High-sensitivity detection of formic acid gas is crucial for industrial safety and environmental monitoring, yet temperature fluctuations often distort sensor responses and compromise measurement accuracy. Here, we present a chitosan-coated whispering-gallery-mode (WGM) microbubble resonator for formic acid sensing with effective temperature compensation. Two compensation strategies, namely fiber Bragg grating (FBG)-assisted correction and modal decoupling between the fundamental and higher-order WGMs, are implemented on the same platform and comparatively evaluated. The FBG-assisted strategy achieves superior accuracy, with a root mean square error (RMSE) of 0.99 ± 0.01 ppm, whereas the modal decoupling strategy yields an RMSE of 2.48 ± 0.67 ppm while offering improved compactness by avoiding additional optical components. The gas sensor exhibits a sensitivity of 3.34 pm/ppm over a concentration range of 0–120 ppm and an experimental limit of detection (LoD) of 1 ppm, together with excellent long-term stability, repeatability, and selectivity. These results demonstrate a practical and compact route for high-precision formic acid sensing under temperature-varying conditions, with promising prospects for environmental monitoring and industrial process control.
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DOI
高影响因子
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