PEST MANAGEMENT SCIENCE
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2015
·Vol. 72
·期 5
·997-1003
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IF 2.694
Xiaoyu Liang,Yabing Duan,Xiaoyue Yu,Jianxin Wang,Mingguo Zhou
BACKGROUND Bismerthiazol is a commonly used bactericide against rice bacterial leaf blight in China. Although previous research determined that bismerthiazol is susceptible to photolytic degradation, the photodegradation pathway and degradation products, except for 2-amino-5-mercapto-1,3,4-thiadiazole, have remained unknown. RESULTS The photodegradation of bismerthiazol was investigated after 4 and 8 h of irradiation in a solar simulator. Inhibition of Xanthomonas oryzae pv. oryzae ( Xoo ) was greater with a photolysed solution than with a non-photolysed solution of bismerthiazol. Six photoproducts of bismerthiazol were characterised by liquid chromatography coupled with mass spectrometry, and, based on these products, a photodegradation pathway was inferred. Inhibition of Xoo was significantly greater with bismerthiazol and 2-amino-5-mercapto-1,3,4-thiadiazole than with 5-amino-1,3,4-thiadiazole. In addition, Xoo strain 2-1-1 was bismerthiazol- and 2-amino-5-mercapto-1,3,4-thiadiazole resistant in vivo . CONCLUSION Photodegradation increased the inhibitory activity of bismerthiazol against Xoo . The photodegradation pathway was inferred on the basis of the photoproducts of bismerthiazol. In vitro assays indicated that the sulfhydryl group was crucial for the inhibition of Xoo by bismerthiazol and its photoproducts. Bismerthiazol and 2-amino-5-mercapto-1,3,4-thiadiazole might have a similar mode action in vivo and in vitro . © 2015 Society of Chemical Industry
研究领域:
植物病理学
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农药化学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
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IF 4.192
Shuangshuang Zhao,Cheng Xiao,Jiajie Wang,Kailin Tian,Wenxia Ji,Tingting Yang,Babar Khan,Guoliang Qian,Wei Yan,Yonghao Ye
β-Ketoacyl-acyl carrier protein synthase III (KAS III, FabH) is essential for bacterial fatty acid biosynthesis. Recent studies indicate that FabH can be a potential target for bactericide development. In the present study, an immobilized FabH column was developed and used to screen FabH inhibitors from complex natural product extracts. Combined with HPLC, four secondary metabolites, alternariol (1), altenuisol (2), alterlactone (3), and dehydroaltenusin (4), were site-directed, isolated, and identified from the crude extract of Alternaria alternata ZHJG5. These compounds showed inhibitory activities on FabH of Xanthomonas oryzae pv. oryzae (Xoo) with IC50 values from 29.5 to 74.1 μM and also displayed a varying degree of antibacterial activities against Xoo with minimal inhibitory concentration values from 4 to 64 μg/mL. Molecular modeling was then used to picture how the compounds interact with XooFabH. Two inhibitors, compounds 1 and 3, exhibited significant bactericidal activity against rice bacterial leaf blight with a protective efficiency of 66.2 and 82.5% at the concentration of 200 μg/mL, respectively, suggesting that they could be lead candidates to develop novel bactericides.
研究领域:
微生物学
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药理学
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植物病理学
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生物化学
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DOI
PubMed
高影响因子
MOLECULES
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2020
·Vol. 25
·期 20
·4795
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IF 3.267
Yasmine Abdallah,Mengju Liu,Solabomi Olaitan Ogunyemi,Temoor Ahmed,Hatem Fouad,Amro Abdelazez,Chenqi Yan,Yong Yang,Jianping Chen,Bin Li
Bacterial leaf blight caused byXanthomonas oryzaepv.oryzae(Xoo) is one of the most devastating diseases, resulting in significant yield losses in rice. The extensive use of chemical antibacterial agents has led to an increase the environmental toxicity. Nanotechnology products are being developed as a promising alternative to control plant disease with low environmental impact. In the present study, we investigated the antibacterial activity of biosynthesized chitosan nanoparticles (CSNPs) and zinc oxide nanoparticles (ZnONPs) against rice pathogen Xoo. The formation of CSNPs and ZnONPs in the reaction mixture was confirmed by using UV-vis spectroscopy at 300–550 nm. Moreover, CSNPs and ZnONPs with strong antibacterial activity againstXoowere further characterized by scanning and transmission electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction. Compared with the corresponding chitosan and ZnO alone, CSNPs and ZnONPs showed greater inhibition in the growth of Xoo, which may be mainly attributed to the reduction in biofilm formation and swimming, cell membrane damage, reactive oxygen species production, and apoptosis of bacterial cells. Overall, this study revealed that the two biosynthesized nanoparticles, particularly CSNPs, are a promising alternative to control rice bacterial disease.Keywords:antibacterial activity;chitosan nanoparticles;green synthesis;ZnO nanoparticles;tomato extract
研究领域:
纳米技术
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植物病理学
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环境科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2024
·Vol. 25
·期 22
·12236
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IF 4.9
Hai-Long Yu,Xiao-Long Liang,Zhen-Yang Ge,Zhi Zhang,Yao Ruan,Hao Tang,Qing-Ye Zhang
Bacterial blight (BB) of rice caused byXanthomonas oryzaepathovar oryzae (Xoo) is a serious global rice disease. Due to increasing bactericide resistance, developing new inhibitors is urgent. Drug repositioning offers a potential strategy to address this issue. In this study, we integrated transcriptional data into a genome-scale metabolic model (GSMM) to screen novel anti-Xootargets. Two RNA-seq datasets (before and after bismerthiazol treatment) were used to constrain the GSMM and simulate metabolic processes. Metabolic fluxes were calculated using parsimonious flux balance analysis (pFBA) identifying reactions with significant changes for target screening. Glutathione oxidoreductase (GSR) was selected as a potential anti-Xootarget and validated through antibacterial experiments. Virtual screening based on the target identified DB12411 as a lead compound with the potential for new antibacterial agents. This approach demonstrates that integrating metabolic networks and transcriptional data can aid in both understanding antibacterial mechanisms and discovering novel drug targets.
研究领域:
微生物学
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生物信息学
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植物病理学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Xiang Zhou,Yu-Mei Feng,Pu-Ying Qi,Wu-Bin Shao,Zhi-Bing Wu,Li-Wei Liu,Yi Wang,Hao-Dong Ma,Pei-Yi Wang,Zhong Li,Song Yang
Xanthomonas oryzae pv. oryzae (Xoo) is an offensive phytopathogen that can invade a wide range of plant hosts to develop bacterial diseases, including the well-known rice bacterial leaf blight. However, few agrochemicals have been identified to effectively prevent and eliminate Xoo-induced diseases. Thus, designing novel antibacterial compounds on the basis of the potential targets from Xoo may lead to the discovery of highly efficient and innovative anti-Xoo agents. Filamentous temperature-sensitive protein Z (FtsZ), an important functional protein in the progression of cell division, has been widely reported and exploited as a target for creating antibacterial drugs in the field of medicine. Therefore, the fabrication of innovative frameworks targeting XooFtsZ may be an effective method for managing bacterial leaf blight diseases via blocking the binary division and reproduction of Xoo. As such, a series of novel N-(cinnamoyl)-N′-(substituted)acryloyl hydrazide derivatives containing pyridinium moieties were designed, and the anti-Xoo activity was determined. The bioassay results showed that compound A7 had excellent anti-Xoo activity (EC50 = 0.99 mg L–1) in vitro and distinct curative activity (63.2% at 200 mg L–1) in vivo. Further studies revealed that these designed compounds were XooFtsZ inhibitors, validating by the reduced GTPase activity of recombinant XooFtsZ, the nonfilamentous XooFtsZ assembly observed in the TEM images, and the prolonged Xoo cells from the fluorescence patterns. Computational docking studies showed that compound A7 had strong interactions with ASN34, GLN193, and GLN197 residues located in the α helix regions of XooFtsZ. The present study demonstrates the developed FtsZ inhibitors can serve as agents to control Xoo-induced infections.
研究领域:
植物病理学
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分子生物学
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药物化学
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DOI
PubMed
高影响因子
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