CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Junjun Liu,Naibo Feng,Xinyu Jiang,Xirui Xin,Yubo Hou,Zhende Jiang,Laijin Lu,Weixian Yu,Bai Yang
Periodontitis is a common chronic inflammatory disease and the local accumulation of excessive reactive oxygen species (ROS) could cause oxidative damage to periodontal tissues. Thus, antioxidative therapy is considered to be the promising strategy for treating periodontitis. However, highly efficient antioxidant activity of biomaterials with low cost, ease of synthesis, and good biocompatibility are still highly desired. Here amylopectin from maize (AFM)-derived carbonized polymer dots (AFM-CPDs-240) with excellent antioxidant activity, good water solubility and biosafty is parepared. Detailed characterizations identify that the antioxidant properties and formation mechanism of AFM-CPDs, and antioxidant activity of AFM-CPDs-240 is much higher than Trolox (a hydrophilic derivative of vitamin E) due to the large contents of conjugated C=C and C=O. Besides, in vitro and in vivo experiments suggest AFM-CPDs-240 could effectively alleviate inflammatory response, inhibit alveolar bone resorption and promote bone formation, showing excellent therapeutic effects on periodontitis. Moreover, the label-free proteomics and western blot results demonstrate AFM-CPDs-240 could accelerate the recovery of periodontitis by blocking TLR4/NF-κB (Toll-like receptor 4/ nuclear factor-kappa B) signaling pathway. This study will develop a simple approach to design highly efficient antioxidant carbon dots with good biocompatibility, and also provide a new promising nanoplatform for treating diseases associated with ROS.
研究领域:
牙医学
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材料科学
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生物化学
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药理学
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DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2020
·
IF 3.503
Shi-Yu Zhang,Ze-Wang Guo,Xiao-Ling Wu,Xiao-Yang Ou,Min-Hua Zong,Wen-Yong Lou
Cold-adapted pullulanase with high catalytic activity and stability is of special interest for its wide application in cold starch hydrolysis, but few pullulanases displaying excellent characteristics at ambient temperature and acidic pH have hitherto been reported. Here, a novel pullulanase from Bacillus methanolicus PB1 was successfully expressed in Escherichia coli BL21 (DE3) and determined to be a cold-adapted type I pullulanase (PulPB1) with maximum activity at 50 °C and pH 5.5. The recombinant PulPB1 showed great stability, its half-life at 50 °C was 137 h. PulPB1 can efficiently hydrolyze pullulan and amylopectin, with activities of 292 and 184 U/mg at 50 °C and pH 5.5, respectively. Moreover, the N-terminal domain of PulPB1 was found to significantly affect the enzymatic performance. Following truncation of the N-terminal domain, the activity towards pullulan decreased markedly from 292 to 141 U/mg and the half-life at 50 °C decreased from 137 to 10 h. Compared to the hydrolysis system with amyloglucosidase alone, the catalytic efficiency showed a 2.4-fold increase on combining PulPB1 with amyloglucosidase for amylopectin hydrolysis at 40 °C. This demonstrates that PulPB1 is promising for development as a superior candidate for cold amylopectin hydrolysis.
研究领域:
生物化学
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微生物学
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酶学
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生物技术
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yue Zhang,Wenjun Song,Yakun Tang,Dianzeng Jia,Yudai Huang
Na3V2(PO4)2F3 is one of the most studied polyanion type cathode materials for sodium-ion batteries (SIBs) and offers great promises. However, the inferior rate capability induced by its sluggish diffusion of electrons and ions greatly limits the practical application of electrode materials in SIBs. Herein, we develop an efficient method to fabricate in situ carbon-coated Na3V2(PO4)2F3 nanosheets by using cost-effective amylopectin. The amylopectin not only could induce the nucleation of Na3V2(PO4)2F3 along its backbone to form a 2D nanostructure, but also act as a source of amorphous carbon for in situ coating on the active material surface. The composite exhibits extraordinary rate capability (104 mA h g–1 at 40 C, 51 mA h g–1 at 150 C) and desirable cycling stability. Such satisfactory achievements, especially the superior rate performance, should be ascribed to its unique 2D nanostructure which shortens the Na+ diffusion length, and the in situ carbon coating endows the composites with effective electron transport. Even applied to full cells, the obtained devices still display an exceptionally high energy density (94.8 W h kg–1), high power density (7295 W kg–1), and excellent cyclic stability.
研究领域:
材料科学
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储能
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电化学
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纳米材料
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DOI
高影响因子
Foods
·
2026
·Vol. 15
·期 14
·2550
·
IF 6
Yuhan Jiang,Sha Ao,Xianwen Hu,Shilin Liu
Water-in-water (W/W) Pickering emulsions based on aqueous two-phase systems (ATPS) are promising for food applications, but their stabilization is challenged by the ultra-low interfacial tension inherent to ATPSs. This study developed food-grade W/W Pickering emulsions stabilized by bacterial cellulose nanocrystals (BCNCs) and evaluated their ability to regulate starch digestibility. The effects of dextran (Dex) concentration, maltodextrin (MD) concentration, and BCNC content on the microstructure, rheological properties, and stability of the emulsions were systematically investigated. The partitioning behavior of amylopectin (AMP) between the two phases and the in vitro digestion behavior of AMP-loaded W/W Pickering emulsions in the presence of α-amylase were further examined. Fourier-transform infrared spectroscopy confirmed the successful preparation of BCNCs. The optimal formulation (24 wt% Dex, 14 wt% MD, and 0.24 wt% BCNCs) exhibited a uniform droplet distribution and enhanced storage stability. Contact angle analysis indicated preferential wettability of BCNCs toward the continuous Dex-rich phase, promoting interfacial adsorption and droplet stabilization. The emulsions remained stable at pH 3.0–7.0 but were sensitive to ionic strength above 7 mM sodium chloride. Importantly, in vitro digestion showed that BCNC-stabilized emulsions significantly inhibited α-amylase-mediated hydrolysis of AMP, with the hydrolysis extent reducing from ~54% to 14%. These results indicate that BCNCs act as effective natural stabilizers for W/W Pickering emulsions, forming a physical barrier that retards starch digestion. This work provides a sustainable strategy for designing slow-digestible, low-glycemic-index food systems with potential applications in functional foods and pharmaceuticals.
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Fang Zhu,Yanru Hu,Lihui Meng,Wenchao Li,Bin Xie,Zilin Zhou,Shuojie Cui,Meng Wang,Youfa Wang,Zebin Chen,Qingzhi Wu
The preparation of biodegradable and antibacterial hydrogels has important clinical value. In this work, a novel strategy has been developed to prepare degradable hydrogel dressings without chemical crosslinking agent using methacrylate anhydride (MA)-modified amylopectin (APMA) and polyacrylamide (PAM). After introducing C C bonds, APMA/PAM hydrogels can be formed under light irradiation. This strategy improves the gelling ability of AP and degradation properties of the hydrogel by avoiding the addition of crosslinking agent. The degradation rate of APMA/PAM hydrogel is 74.04 ± 0.69 % within 12 weeks, while that of APMA/PAM hydrogel containing crosslinking agent is only 38.5 ± 0.1 %. The APMA/PAM hydrogel loading curcumin (Cur) (APMA/PAM-Cur) exhibits high antibacterial efficiency of 98.29 ± 0.41 % and 97.18 ± 0.81 % against S. aureus and E. coli , respectively, with light irradiation. Animal experiments show that the APMA/PAM-Cur hydrogel reduces the infiltration of inflammatory factors, increases the density of collagen, and makes the newly formed granulation tissue thicker and tighter. This study not only proves the promising potential of the APMA/PAM-Cur hydrogel as degradable and antibacterial wound dressing for clinical treatment, but also provides a new strategy for developing low-cost, degradable, and antibacterial wound dressings and reducing antibiotic abuse and environmental pollution caused by medical waste.
研究领域:
生物材料学
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医学工程
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药剂学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 13
·3999
·
IF 4.412
Huiyuan Luo,Fuping Dong,Qian Wang,Yihang Li,Yuzhu Xiong
The performance of hydrogels prepared with traditional natural starch as raw materials is considerable; the fixed ratio of amylose/amylopectin significantly limits the improvement of hydrogel structure and performance. In this paper, starch hydrogels were prepared by physical blending and chemical grafting, with the aid of ultrasonic heating. The effects of different amylose/amylopectin ratios on the microstructure and water retention properties of starch hydrogels were studied. The results show that an increase in amylopectin content is beneficial to improve the grafting ratio of acrylamide (AM). The interaction between the AM grafted on amylopectin and amylose molecules through hydrogen bonding increases the pores of the gel network and thins the pore walls. When the amylopectin content was 70%, the water absorption (swelling 45.25 times) and water retention performance (16 days water retention rate 44.17%) were optimal. This study provides new insights into the preparation of starch-based hydrogels with excellent physical and chemical properties.Keywords:ultrasonic chemistry;hydrogel;starch;porous structure;water-retaining
研究领域:
材料科学
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生物化学
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DOI
PubMed
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
·
2019
·Vol. 305
·期 1
·1900450
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IF 3.038
Yikun Zhang,Dapeng Li,Yiwan Huang,Shijun Long,Haiyan Li,Xuefeng Li
A simple, multiple-hydrogen-bond approach to fabricating physically crosslinked, Amylopectin reinforced polyacrylamide/poly(vinyl alcohol) (Amy/PAAm/PVA) double-network (DN) hydrogels with super toughness in bulk and at solid interfaces is reported. The Amy/PAAm/PVA DN hydrogels exhibit high tensile strength (854.1 kPa), high extensibility (≈eight times), high bulk toughness (4094.8 kJ m −3 ), good self-recovery property (≈92% of self-recovery at room temperature), and strong adhesion to nonporous glass surfaces (≈158 kPa). Such tough and adhesive DN hydrogels have great potential for various applications in engineering artificial soft tissues, flexible electronics, and wearable devices.
研究领域:
材料科学
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高分子化学
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生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2020
·Vol. 56
·期 2
·639-647
·
IF 2.773
Qi Li,Shaoxia Shi,Yaoyao Dong,Xiuzhu Yu
Summary Frying is commonly used to process starchy foods. This work explored the interactions of amylose (AM) and amylopectin (AP) with lipids during frying. Maize-derived AM and AP that were adjusted to various moisture contents were fried at 180 °C. The significant effects of moisture content and formation of starch–lipid complexes were confirmed. Rheological analysis indicated that the elasticity of the fried AM paste decreased. This change was observed in AM but not in AP. Viscoelasticity decreased as the initial starch moisture content was increased. The presence of a V-type structure was confirmed by XRD. In general, relative crystallinity decreased as moisture content was increased. On the other hand, the increasement of starch–lipid complex could elevated the degree of crystallisation. AM was more likely than AP to undergo complexation with lipids during frying. Increasing the moisture content of starch within 20%~60% promoted the formation of starch–lipid complexes.
研究领域:
食品科学
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化学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Feng-Ping Wang,Bo Li,Mei-Yan Sun,Fazli Wahid,Hong-Mei Zhang,Shu-Jun Wang,Yan-Yan Xie,Shi-Ru Jia,Cheng Zhong
Bacterial cellulose (BC) is a promising biopolymer, but its three-dimensional structure needs to be controllable to be used in multiple fields. BC has some advantages over other types of cellulose, not only in terms of purity and properties but also in terms of modification (in situ modification) during the synthesis process. Here, starches from different sources or with amylose/amylopectin content were added to the growth medium to regulate the structural properties of BC in-situ. The obtained BC membranes were further modified by superhydrophobic treatment for oil-water separation. Starches alter the viscosity of the medium, thus affecting bacterial motility and cellulose synthesis, and adhere to the microfibers, limiting their further polymerization and ultimately altering the membrane porosity, pore size, and mechanical properties perpendicular to the BC fibril layer direction. The average pore diameter of the BC/PS membrane increased by 1.94 times compared to the initial BC membrane. The chemically modified BC/PS membrane exhibited super-hydrophobicity (water contact angle 167°), high oil-water separation flux (dichloromethane, 23,205 Lm −2 h −1 MPa −1 ), high separation efficiency (>97%). The study provides a foundation for developing methods to regulate the network structure of BC and broaden its application.
研究领域:
生物技术
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材料科学
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环境工程
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产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
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2022
·
IF 5.546
Yan Gao,Yi Wang,Yuyin Dai,Qi Wang,Peng Xiang,Yinghua Li,Guanghui Gao
Anti-swelling hydrogels have been used as underwater sensors, underwater actuators and artificial biomimetic cartilage. In order to improve the anti-swelling property, most hydrogels introduce environment-unfriendly chemical crosslinkers or organic solvents into the polymer network to enhance the crosslinking density of the network, which limits their application in the body. Therefore, we settled a dense hydrogen-bond network by introducing hyperbranched amylopectin (Amy) into polyvinyl alcohol (PVA) to prepare a non-toxic, biocompatible, tough hydrogel sensor. In addition, the hydrogels exhibit significant anti-swelling performance in various liquid media (such as swelling rates = 3.55%, in seawater) and excellent mechanical properties due to strong intermolecular hydrogen bond between Amy and PVA as well as the salting-out effect induced by sodium chloride (NaCl). Moreover, the hydrogels show moderate sensitivity (GF = 4.96), rapid response, signal stability and negligible hysteresis, which can be used to detect a variety of physiological signals and human movements. More significantly, the hydrogels have excellent biocompatibility and potential application prospects in the implantable sensors and medical fields.
研究领域:
材料科学
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生物医学工程
·
传感器技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shaomin Kang,Zhenghong Wu,Yena Liu,Peipei Wang,Xinyu Zhang,Mehraj Ahmad,Mohammad Rizwan Khan,Wenyuan Zhu,Jiaqi Guo,Yongcan Jin,Huining Xiao,Junlong Song
Cationic starch is the most widely used paper strength additive for papermaking wet end applications. However, it remains unclear how differently quaternized amylose (QAM) and amylopectin (QAP) are adsorbed on the fiber surface and their relative contribution to the inter-fiber bonding of papers. Herein, separated amylose and amylopectin were quaternized with different degrees of substitution (DS). After that, the adsorption behaviors of QAM and QAP on the fiber surface, the viscoelastic properties of the adlayers and their strength enhancement to fiber networks were comparatively characterized. Based on the results, the morphology visualizations of the starch structure displayed a strong impact on the adsorbed structural distributions of QAM and QAP. QAM adlayer with a helical linear or slightly branched structure was thin and rigid, while the QAP adlayer with a highly branched structure was thick and soft. In addition, the DS, pH and ionic strength had some impacts on the adsorption layer as well. Regarding the paper strength enhancement, the DS of QAM correlated positively to the paper strength, whereas the DS of QAP correlated inversely. The results provide a deep understanding of the impacts of starch morphology on performance and offer us some practical guidelines in starch selection.
研究领域:
造纸科学
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaoxiao Li,Tong Jiang,Yu Wang,Jingjing Dong,Zhengyu Jin,Yuxiang Bai
Limosilactobacillus reuteri 121 4,6-α-glucanotransferase (GtfBΔN) modifies starch by cleaving (α1 → 4) linkages and introducing non-branched (α1 → 6) linkages to produce functional starch derivatives. Research has mainly focused on GtfBΔN converting amylose (linear substrate), whereas the conversion of amylopectin (branched substrate) has not been studied in detail. In this study, we used GtfBΔN to understand amylopectin modification and performed a set of experiments to analyze this modification pattern. The donor substrates were segments from the non-reducing ends to the nearest branch point in amylopectin as shown from the results of the chain length distribution of GtfBΔN-modified starches. Decreased and increased contents of β-limit dextrin and reducing sugars, respectively, during the incubation of β-limit dextrin with GtfBΔN indicated that the segments from the reducing end to the nearest branch point in amylopectin act as donor substrates. Dextranase was involved in the hydrolysis of the GtfBΔN conversion products of three different substrates groups, maltohexaose (G6), amylopectin, and G6 plus amylopectin. No reducing sugars were detected, therefore, amylopectin was not used as an acceptor substrate, and no non-branched (α1 → 6) linkages were introduced into it. Thus, these methods provide a reasonable and effective approach to studying GtfB-like 4,6-α-glucanotransferase in analyzing the roles and contribution of branched substrates.
研究领域:
生物化学
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微生物学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）