JOURNAL OF SEPARATION SCIENCE
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2015
·Vol. 39
·期 4
·648-654
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IF 2.737
Chao-Xi Zeng,Rui-Pu Xin,Sui-Jian Qi,Bo Yang,Yong-Hua Wang
Aqueous two-phase systems, based on the use of natural quaternary ammonium compounds, were developed to establish a benign biotechnological route for efficient protein separation. In this study, aqueous two-phase systems of two natural resources betaine and choline with polyethyleneglycol (PEG400/600) or inorganic salts (K 2 HPO 4 /K 3 PO 4 ) were formed. It was shown that in the K 2 HPO 4 -containing aqueous two-phase system, hydrophobic interactions were an important driving force of protein partitioning, while protein size played a vital role in aqueous two-phase systems that contained polyethylene glycol. An extraction efficiency of more than 90% for bovine serum albumin in the betaine/K 2 HPO 4 aqueous two-phase system can be obtained, and this betaine-based aqueous two-phase system provided a gentle and stable environment for the protein. In addition, after investigation of the cluster phenomenon in the betaine/K 2 HPO 4 aqueous two-phase systems, it was suggested that this phenomenon also played a significant role for protein extraction in this system. The development of aqueous two-phase systems based on natural quaternary ammonium compounds not only provided an effective and greener method of aqueous two-phase system to meet the requirements of green chemistry but also may help to solve the mystery of the compartmentalization of biomolecules in cells.
研究领域:
生物化学
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环境科学
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分子生物学
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DOI
PubMed
高影响因子
TALANTA
·
2013
·
IF 3.498
Qun Zeng,Yuzhi Wang,Na Li,Xiu Huang,Xueqin Ding,Xiao Lin,Songyun Huang,Xiaojie Liu
Eight kinds of green ionic liquids were synthesized, and an ionic liquid aqueous two-phase system (ILATPS) based on 1,1,3,3-tetramethylguandine acrylate (TMGA) guanidine ionic liquid was first time studied for the extraction of proteins. Single factor experiments proved that the extraction efficiency of bovine serum albumin (BSA) was influenced by the mass of IL, K 2 HPO 4 and BSA, also related to the separation time and temperature. The optimum conditions were determined through orthogonal experiment by the five factors described above. The results showed that under the optimum conditions, the extraction efficiency could reach up to 99.6243%. The relative standard deviations (RSD) of extraction efficiencies in precision experiment, repeatability experiment and stability experiment were 0.8156% ( n =5), 1.6173% ( n =5) and 1.6292% ( n =5), respectively. UV–vis and FT-IR spectra confirmed that there were no chemical interactions between BSA and ionic liquid in the extraction process, and the conformation of the protein was not changed after extraction. The conductivity, DLS and TEM were combined to investigate the microstructure of the top phase and the possible mechanism for the extraction. The results showed that hydrophobic interaction, hydrogen bonding interaction and the salt out effect played important roles in the transferring process, and the aggregation and embrace phenomenon was the main driving force for the separation. All these results proved that guanidine ionic liquid-based ATPSs have the potential to offer new possibility in the extraction of proteins.
研究领域:
生物化学
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蛋白质工程
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-12
·
IF 17.694
Lu Ye,Jiang Longlong,Yu Yang,Wang Dehua,Sun Wentao,Liu Yang,Yu Jing,Zhang Jun,Wang Kai,Hu Han,Wang Xiao,Ma Qingming,Wang Xiaoxiong
Solid nanogenerators often have limited charge transfer due to their low contact area. Liquid–liquid nanogenerators can transfer a charge better than the solid–solid and solid–liquid counterparts. However, the precise manipulation of the liquid morphology remains a challenge because of the fluidity limits of the liquid. In this work, using the surface tension of a droplet to fix its shape, a liquid-liquid triboelectric nanogenerator in Contact-Separation mode is designed using an immiscible aqueous-aqueous interface, achieving a contact surface charge transfer of 129 nC for a single droplet. The configuration is proven to be applicable in humid environments, and the two-phase materials have good biocompatibility and can be used as an effective drug carrier. Therefore, this nanogenerator is useful for designing future implantable devices. Meanwhile, this design also establishes the foundation of aqueous electronics, and additional applications can be achieved using this route.
研究领域:
纳米技术
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材料科学
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生物医学工程
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电子学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 94
·期 7
·2229-2237
·
IF 2.659
Xiaoqi Yi,Xiaojing Mu,Gang Chen,Yan Gou,Chengshan Wang,Xiaolin Yuan
Background Aqueous two-phase extraction has advantages of green extraction, high yield, good product purity and low cost. However, conventional aqueous two-phase systems (ATPSs) are limited in extraction/separation due to high polarity. Some researchers tuned the hydrophobicity of ATPSs by addition of hydrophobic ionic liquids or polymers. We aimed to tailor the hydrophobicity of a solvent–salt–water ATPS using cosolvent for extraction of paeonol from cortex moutan . Results Tie-line length and compositions in the ATPS were employed for qualitative evaluation of polarity and polarity difference between the two phases. Conditions such as pH, salt type and concentration, total solvent volume fraction and cosolvent fraction were optimized. Ethyl acetate (EA) as cosolvent in ethanol–NaH 2 PO 4 –H 2 O (EtPO) system did not alter the first-order kinetic extraction model, but it improved the kinetic constant and boosted the partition coefficient from 36.7 to 58.8. For both EtPO and EtPO–EA systems, both the extraction yields and partition coefficients increased when temperature increased from 11 to 37 °C. The optimized EtPO–EA system demonstrated higher extraction yield than EtPO and 70% (v/v) ethanol systems by 25.4 and 19.5%, respectively. Conclusions The hydrophobicity of an ATPS was successfully tuned with cosolvent for paeonol extraction. The cosolvent–salt–water two-phase systems are promising in extraction of weakly polar components from herbs. © 2019 Society of Chemical Industry
研究领域:
化学工程
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药学
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分析化学
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产品关联
DOI
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
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2018
·
IF 1.2
Yajuan Li,Yanyang Wu,Kui Chen,Bin Wu,Lijun Ji,Jiawen Zhu
A polymer–salt aqueous two-phase system based on polyethylene glycol and sulfates was applied to explore the partition behavior of spiramycin. Binodal curves were measured by the turbidimetric titration method. The influence of temperature, salt concentration, and pH on the distribution behavior was investigated in detail by determining the partition coefficient and extraction efficiency. As pH and salt concentration increase, the partition coefficients increase accordingly. It was found that pH displays a significant influence on the partition behavior. The partition coefficient and extraction efficiency can reach 42.46 and 97.8%, respectively, at pH 9.0 and 15.80% (w/w) salt concentration.
研究领域:
生物化学
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制药工程
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产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 12
·4067-4076
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IF 1.938
Jing Chen,Yuzhi Wang,Qun Zeng,Xueqin Ding,Yanhua Huang
A series of hydroxyl ammonium ionic liquids (ILs) have been designed and synthesized. An ionic liquid aqueous two-phase system based on N,N-dimethylethanolamine propionate ([DMEA][Pr]) ionic liquid was studied for the extraction of proteins. Based on the single-factor experiment, an initial serial investigative test was used to identify the optimal conditions. Five factors and four levels of orthogonal experiments were used to verify the optimum extraction conditions. The results show that under the optimum conditions, the extraction efficiency could reach up to 99.50%. The RSD of extraction efficiencies in precision experiment, repeatability experiment and stability experiment were 0.3% (n = 5), 1.1% (n = 5) and 1.5% (n = 5), respectively. The conformation of the proteins was not affected after extraction into the IL-rich phase in terms of the determination by UV-vis and FT-IR spectra. According to the determination of conductivity, dynamic light scattering, and transmission electron microscope images, the microstructure of the IL-rich phase and the possible mechanism for the extraction are investigated. Hydrogen bonding interaction, salt out effect and the aggregation phenomenon played important roles in the extraction. The circular dichroism spectral experiment analysis indicated that the secondary structures of the protein were unchanged after extract. Based on these findings, it is suggested that the method of hydroxyl ammonium-based ILs-ATPs have the potential to offer new possibility in the extraction of bio-analytes.
研究领域:
生物化学
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分析化学
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产品关联
DOI
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-11
·
IF 17.694
Xiao Yang,Ribe Neil M.,Zhang Yage,Pan Yi,Cao Yang,Shum Ho Cheung
The solutal Marangoni effect is attracting increasing interest because of its fundamental role in many isothermal directional transport processes in fluids, including the Marangoni-driven spreading on liquid surfaces or Marangoni convection within a liquid. Here we report a type of continuous Marangoni transport process resulting from Marangoni-driven spreading and Marangoni convection in an aqueous two-phase system. The interaction between a salt (CaCl 2 ) and an anionic surfactant (sodium dodecylbenzenesulfonate) generates surface tension gradients, which drive the transport process. This Marangoni transport consists of the upward transfer of a filament from a droplet located at the bottom of a bulk solution, coiling of the filament near the surface, and formation of Fermat’s spiral patterns on the surface. The bottom-up coiling of the filament, driven by Marangoni convection, may inspire automatic fiber fabrication.
研究领域:
流体动力学
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材料科学
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表面化学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 16
·5288
·
IF 4.927
Bangfu Liu,Zhijian Tan
(1) Background: Aqueous two-phase systems (ATPSs) have been widely used in the separation and purification of bioactive substances in recent years. (2) Methods: In this study, deep eutectic solvents (DESs)-based ATPSs were employed for the extraction and separation ofAstragalus membranaceuspolysaccharides (AMP). The optimal DES (choline chloride:urea = 1:1) was first screened to extract AMP, and the effect of DES concentration, solid–liquid ratio, pH, extraction temperature, and extraction time on the extraction yield of AMP were investigated. (3) Results: The maximum extraction yield was 141.11 mg/g under the optimum conditions. AMP was then preliminarily purified by ATPS, to further realize the recycling and reuse of DES. The effect of type of salts, salt concentration, and extraction temperature on extraction efficiency was investigated. The extraction efficiency was 97.85% for AMP under the optimum ATPS conditions. Finally, the obtained AMP was studied by molecular weight determination, infrared spectroscopy analysis, and monosaccharide composition analysis. (4) Conclusions: This ATPS extraction based on DESs is simple, environmentally friendly, low-cost, and has high extraction efficiency, which provides new ideas for the extraction of plant polysaccharides and other bioactive compounds.Keywords:Astragalus membranaceuspolysaccharides;deep eutectic solvents;solid–liquid extraction;aqueous two-phase system;separation and purification
研究领域:
植物化学
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生物分离工程
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Kai Xie,JunFen Wan,Xuejun Cao
Cefprozil is a second-generation cephalosporin antibiotic, is widely used in the treatment of bronchitis and bacterial infections. Chemical synthesis methods have the disadvantage of complicated steps and high toxicity, while enzymatic synthesis in aqueous phase can be incomplete and susceptible to side reactions. To address these issues, a new green preparation method for cefprozil was developed by combining an enzymatic reaction with a 20 % ethylene oxide/80 % propylene oxide (v/v) random copolymer (EO 20 PO 80 )/water aqueous two-phase system (ATPS). In this method, the yield of the enzyme reaction increased from 80.3 % in the aqueous system to 93.1 %. The resulting products were mainly precipitated, with a small amount remaining in the two-phase solution. The distribution coefficient was 1.44. After washing and purification , the purity of the precipitated product reached 97.3 %, with no remaining polymer. The product in the two-phase solution was purified by crystallization using DMF . The total recovery of the precipitated and solution products after crystallization was 78.9 %. Finally, molecular simulation technology was used to explain how polymers promote cefprozil precipitation at a microscopic level.
研究领域:
生物化学
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制药工程
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产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 16
·5284
·
IF 4.927
Peipei Yang,Mengya Lu,Jing Zhao,Emelda Rosseleena Rohani,Rongchun Han,Nianjun Yu
By applying the hydrophilic ionic liquid, 1-butyl-3-methylimidazolium chloride ([C4mim]Cl), and inorganic salts (K3PO4), an ionic liquid aqueous two-phase system (ILATPS) was established for the separation ofDendrobium huoshanensepolysaccharides (DhPs) and proteins. The effects of inorganic salt concentration, IL quantity, crude DhPs concentration, pH value and temperature were studied to achieve the optimal condition. With the best combination of ILATPS (1.75 g K3PO4, 1.25 g [C4mim]Cl, 10 mg crude DhPs and 5.0 mL ddH2O at pH 7.0 under 25 °C), the extraction efficiency rates for DhPs and proteins were 93.4% and 90.2%, respectively. The processed DhPs retrieved from the lower salt-rich phase comprised mannose, glucose, galactose, arabinose, and galacturonic acid with a molar ratio of 185:71:1.5:1:1 and the molecular weight was 2.14 × 105Da. This approach is fast, simple and environmentally friendly. It provides a new insight into purifying functional polysaccharides of plant origin.Keywords:Dendrobium huoshanense;ionic liquid;polysaccharides;aqueous two-phase system
研究领域:
化学工程
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生物技术
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药学
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DOI
PubMed
高影响因子
Journal of Applied Spectroscopy
·
2014
·Vol. 81
·期 5
·807-811
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IF 0.514
Liu Y.,Fan J.,Ma W.,Fu Ch.
An aqueous two-phase system of acetone and (NH 4 ) 2 SO 4 mixture has been developed for the extractive separation of copper (Cu). The complex formed by Cu(II) and Nuclear Fast Red was found to be extracted into the acetone-rich upper phase, and the absorbance of the complex was determined at 465 nm. Beer’s law was obeyed over the concentration range of ~0.6–3.2 mg/l for Cu(II) with a good linearity. The proposed method has been applied to the separation and determination of Cu(II) in seafood samples with a coefficient of variation less than 2.8%. The recovery obtained was ~96.3–101.7%.
研究领域:
分析化学
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环境化学
·
食品安全检测
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiajun Li,Hui Yang,Yun-Yi Wei,Hai-Yan Wen,Zhao-Long Wu,Huiping Xia,Ditpon Kotatha,Bing-Zheng Li,Liming Che
It is challenging to fabricate uniform starch microspheres (SMs) with sub-10 μm diameters in starch-polyethylene glycol (PEG) aqueous two-phase system (ATPS). To address this issue, a refined approach using starch-polyvinylpyrrolidone (PVP) ATPS is presented in this work. Phase diagrams were constructed for ATPSs containing PVPs of varying molecular weights, which provided crucial insights into the influence of PVP on SM formation. The results revealed that increasing both the molecular weight and concentration of PVP led to higher starch and PVP contents within the dispersed phase, significantly influencing the final yield of SMs. The morphology, size, crystallinity, and swelling behavior of SMs prepared in the starch-PVP ATPS were comprehensively characterized. Increasing PVP molecular weight resulted in smaller SMs with enhanced crystallinity and reduced swelling capacity. Notably, SMs prepared with 58 kDa PVP exhibited an average particle size ( D [4,3 ) of 5.13 μm, a narrow size distribution (Span value = 1.33), a B + V crystal type, and a relative crystallinity of 20.9 %. Furthermore, no residual PVP was detected in the final SMs. This innovative starch-PVP ATPS approach offers a promising route for the controlled fabrication of sub-micron SMs with tightly controlled size distributions. This method holds significant potential for applications demanding well-defined starch-based materials in various fields. This innovative starch-PVP ATPS approach offers a promising route for controlled fabrication of uniform SMs with sub-10 μm diameters, which could be advantageous for drug encapsulation and delivery systems requiring high structural stability.
研究领域:
材料科学
·
化学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）