RSC Advances
·
2015
·Vol. 6
·期 1
·515-521
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IF 3.84
Junyan Yao,Shijie Zhang,Wudan Li,Zhi Du,Yujie Li
Poly(lactic acid-co-lysine) (PLL) was applied as a modified material to improve the hydrophilicity, reactivity, and degradation rate of poly(L-lactic acid) (PLLA). In the drug delivery system, PLLA and PLLA/PLL were selected as the carrier materials of drug-loaded fibers, and bacitracin (BAC) as the sustained release drug. Uniaxial drug-loaded fibers (PLLA/BAC, PLLA/PLL/BAC) and coaxial drug-loaded fibers ((PLLA/PLL/BAC)-PLLA, (PLLA/BAC)-(PLLA/PLL)) were prepared using electrospinning. The chemical structures and thermal properties of the electrospun drug-loaded fibers were analyzed using Infrared Spectroscopy (IR) and Differential Scanning Colorimetry (DSC) separately and an Ultraviolet spectrophotometer (UV) was used to characterize the extracorporeal drug releasing behavior. The results indicated that the main interaction between the BAC and carrier materials such as PLLA and PLLA/PLL was physical force; the uniaxial drug-loaded fibers with uniform diameters showed a higher release rate compared with the coaxial drug-loaded fibers owing to the protection of shell materials in the coaxial fibers. The drug release rate of the drug-loaded fibers increased due to the addition of hydrophilic PLL; the drug release mechanism in both the uniaxial and coaxial drug delivery systems could be interpreted by a biexponential-biphase kinetics function. Two kinds of fibers with different drug release patterns could be fabricated by uniaxial and coaxial electrospinning. Drug in uniaxial fibers could be quickly released and was suitable for antibiotic treatment while the release of drug in coaxial fibers was a kind of controlled release which could be applied for a drug delivery system of long-term and small doses.
研究领域:
生物医学工程
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2022
·
IF 0
Tianran Lin,Gaoyan Jiang,Danxuan Lin,Yunping Lai,Li Hou,Shulin Zhao
Bacitracin (an antimicrobial peptide, AMP)-modified dextran-MoSe2 nanosheets (AMP/dex-MoSe2 NSs) were constructed and applied for low-temperature and synergetic antibacterial applications. The near-infrared (NIR) photothermal and peroxidase-like activities of dex-MoSe2 were combined with the bacterial membrane-binding ability of AMP through electrostatic adsorption, and a multimode antibacterial method was realized. H2O2 was converted into a hydroxyl radical (·OH) by AMP/dex-MoSe2, which exhibits a higher antibacterial activity and can avoid the toxicity of a high concentration of H2O2. Importantly, the production of ·OH and the antibacterial efficiency of AMP/dex-MoSe2 were accelerated by low-temperature heat sterilization with NIR irradiation. Owing to the AMP-guided binding and destruction effect to the bacterial membrane, AMP/dex-MoSe2 shows a better antibacterial effect on Gram-negative Escherichia coli under NIR irradiation as compared to catalytic treatment or NIR photothermal sterilization alone. Furthermore, the cytotoxicity and hemolysis of AMP/dex-MoSe2 were weak and in a relatively safe range. This multimode antibacterial strategy based on the AMP/dex-MoSe2 nanozyme will pave a way for the development of more safe and efficient antibacterial applications.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xingdong Yang,He Beibei,Yang Qu,Lihua Wu,Chenchen Wang,Yutong Cao,Yinuo Zhu,Zhenchun Zang,Xiaofei Hu,Lili Li
In recent years, due to the unscientific and irrational use of bacitracin (BAC), the drug resistance of animal-derived bacteria has continued to increase, posing a threat to animal-derived food safety and public health safety. Therefore, there is an urgent need to develop a rapid and sensitive BAC assay. In this study, BAC-ovalbumin was used as the coated antigen, mouse monoclonal antibody was used as the detection antibody, and the working system was optimized. An indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) for the detection of BAC was established. Under the optimal conditions, the half maximal inhibitory concentration of the method was 0.10 ng/mL, and the linear range of detection was 0.05–2.19 ng/mL. Limit of detection of the method was at least 500 times lower than the maximum residue limits. The sample spike recovery experiment revealed that the recovery rates for animal tissue and milk were 78.7 %–93.7 % and 81.3 %–96.7 %, respectively. The intra-assay and interassay coefficients of variation were all less than 15 %, with good precision and no cross-reaction with five bacitracin analogs. At the same time, HPLC also verified the reliability of the method. This method provides a convenient, accurate and rapid means of screening BAC residues in actual animal-derived foods.
研究领域:
分析化学
·
免疫学
·
食品安全
·
兽医学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Shixin Zhang,Xiaomin Tang,Huaili Zheng,Dongsheng Wang,Zongli Xie,Wei Ding,Xinyu Zheng
Bacteria, especially antibiotic-resistant bacteria, in water threaten public health in countries. Simultaneous flocculation , sterilization and identification of bacteria are great challenge in water treatment . Herein we presented a three-in-one compound through combining a novel Bacitracin-based flocculant (B-g-PAMDAC) and surface enhanced Raman scattering (SERS) labels, the modified Au@AgNPs using graphene oxide (GO) and 4-mercaptophenylboronic acid (4-MPBA). B-g-PAMDAC with bactericidal groups and microblock structure was synthesized via copolymerization and self-assembly. Its functional groups and microblock structure contributed to the excellent performance in flocculation of bacteria. 4-MPBA as bacterial capture bound to the bacterial cell membrane and contributed to recognition of bacteria in flocculation. Bacteria aggregating around Au@AgNPs resulted in abundant “hot spots” and strong Raman signals. SERS labels obviously improved the sensitivity, accuracy and stability of bacteria identification even at low bacterial concentration of 1 × 10 3 CFU mL −1 . They presented distinct fingerprints of bacteria, Escherichia coli , Pseudomonas aeruginosa , Bacillus cereus and Enterococcus faecalis , in Raman mappings. Bacitracin improved sterilization efficiency of B-g-PAMDAC in four bacteria treatment in terms of sterilization rate and time. β-galactosidase and respiratory activity of bacteria revealed sterilization mechanism of B-g-PAMDAC that changed permeability of cell membrane before it reduced the respiration activity of bacteria and ruptured cell wall.
研究领域:
环境科学
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）