COLLOIDS AND SURFACES B-BIOINTERFACES
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2020
·
IF 4.389
Fang Wang,Jiarui Li,Xinyue Tang,Kexin Huang,Lan Chen
In this work, dual drug loaded in chitosan/dextran sulfate/chitosan (CS/DEX/CS) nanoparticles was synthesized by layer-by-layer (LBL) self-assembly technique for use in anti-cancer drug delivery. The nanoparticles were characterized in terms of particle size, zeta-potential, encapsulation efficiency and morphology (SEM and TEM). The in vitro release of the dual drugs, inner PTX and outer 5-Fu, from the CS-PTX/EX/CS-5Fu nanoparticles with different numbers of CS and DEX layers and different PBS was characterized. The results revealed that the pH-sensitive dual drug loaded nanoparticles exhibited a controlled release profile, and the release mechanism followed Two-phase kinetic model for PTX and Higuchi model for 5-Fu. Subsequently the cytotoxicity of nanoparticles was evaluated against HepG2 cells using MTT and apoptosis assay, resulting in synergistic effects between dual drugs and enhanced inhibition to cancer cells. Cellular uptake studies demonstrated efficient internalization of PTX and 5-Fu in HepG2 cells. Therefore the dual drug loaded CS/DEX/CS nanoparticles had good prospects for the biomedical delivery application.
研究领域:
药学
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纳米技术
·
癌症研究
·
药物递送系统
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DOI
PubMed
高影响因子
Foods
·
2026
·Vol. 15
·期 13
·2312
·
IF 6
Jinhong Huang,Yiping Wang,Mengyuan Pang,Chengpeng Li,Pengzhi Hong,Zhang Hu
Fresh fruits are prone to spoilage due to microbial contamination and moisture loss, highlighting the need for effective packaging materials with strong barrier and antimicrobial functions. In this work, a bilayer composite film with antimicrobial and preservative properties was fabricated using the solution casting method, consisting of an inner chitosan (CS) layer and an outer complex layer of chitosan-2-pyridinecarboxaldehyde-Ag(I) (CS-PCA-Ag(I)). Preparation conditions were optimized via single-factor experiments combined with response surface methodology. The resulting composite film showed significantly enhanced mechanical properties, with tensile strength of 38.52 ± 2.07 MPa and elongation at break of 67.32 ± 1.47%, as well as a low water vapor permeability of 2.81 × 10−7g·m−1·h−1·Pa−1. It also exhibited strong antibacterial activity againstStaphylococcus aureusandEscherichia coli, with inhibition zone diameters of 19.8 ± 0.3 mm and 15.6 ± 0.2 mm, respectively. Strawberry preservation tests demonstrated that the CS/CS-PCA-Ag(I) film effectively suppressed microbial growth and reduced fruit weight loss (8.27 ± 0.42% after 5 days), thereby extending the shelf life of strawberries. Cytotoxicity evaluation and silver ion migration analysis further confirmed the film’s good biocompatibility and safety. Collectively, the CS/CS-PCA-Ag(I) composite film holds considerable promise for fresh food preservation applications.
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Samah Attia Algharib,Ali Dawood,Kaixiang Zhou,Dongmei Chen,Chao Li,Kuiyu Meng,Muhammad Kashif Maa,Saeed Ahmed,Lingli Huang,Shuyu Xie
Due to the poor solubility and permeability of rifaximin (RFX), it is not effective against intracellular pathogens although it shows strong activity against most bacteria. To develop an effective mucoadhesive drug delivery system with a targeted release in bacterial infection site, RFX-loaded chitosan (CS)/carboxymethyl-chitosan (CMCS) nanogel was designed and systematically evaluated. FTIR, DSC, and XRD demonstrated that the nanogel was formed by interactions between the positively charged NH 3 + on CS and CMCS, and the negatively charged COO on CMCS. RFX was encapsulated into the optimized nanogel in amorphous form. The nanogel was a uniform spherical shape with a mean diameter of 171.07 nm. It had excellent sustained release, strong mucin binding ability, and pH-responsive properties of quicker swelling and release at acidic pH. It showed low hemolytic ratio and high antioxidant activity. The present investigation indicated that the CS-nanogel could be potentially used as a promising bacterial responsiveness drug delivery system.
研究领域:
药剂学
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生物材料
·
感染性疾病
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Xiaogang Gu,Yongxin Tao,Yan Pan,Linhong Deng,Liping Bao,Yong Kong
Inspired by the double helix structure of DNA, a simple enantioselective system based on chitosan (CS) was employed for electrochemical enantiorecognition of tryptophan (Trp) isomers. The recognition mechanism was proposed from the supramolecular point of view, which was further verified by the recognition of Trp isomers with sulfonated CS (SCS). The SCS-based chiral system presented the ability of indicating the percentage of d-Trp in racemic mixture, extending future applications of the electrochemical chiral system based on natural polysaccharides.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Ziru Lian,Rong Pan,Jiangtao Wang
Norfloxacin chitosan/chitosan oligosaccharide microcapsules (NCCM) were prepared by emulsion-chemical crosslinking method. The characteristics of obtained microcapsules were evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy and release experiments. Cumulative release profile of norfloxacin from the chitosan microcapsules in natural seawater was measured and the controlled release of drugs was at a uniform rate in 48 h. The chitosan microcapsules were applied onto the antibacterial study of the shrimp culture in natural seawater. It is observed that the seawater in the NCCM added groups was relatively clear and the biomass of Vibrio increased slowly in contrast to the control and norfloxacin groups. The inhibition rate of Vibrio in norfloxacin groups obvioursly decreased after the 5 th day, whereas, it remained high and stable during experiment period in NCCM groups. The results showed that the chitosan microcapsules as release materials have excellent antibacterial effects on Vibrio in the farming of Penaeus vannamei Boone. The controlled release could obviously reduce dosage of antibiotics and delivery times, and effectively improve the utilization rate of norfloxacin drugs for shrimps
研究领域:
水产养殖学
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药剂学
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微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Linlin Wang,Lilong Wang,Chengrong Wen,Nan Wang,Chunhong Yan,Caihong Shen,Shuang Song
Chitosan (CTS)/chitosan oligosaccharide (COS) and whey protein isolate (WPI) have been frequently used as food supplements, but notably, the interaction between the carbohydrate and the protein may affect the digestibility of protein. Thus, the present study focused on effects of the interaction between CTS/COS and WPI on the protein digestibility. A series of chemical and spectroscopic techniques including gel electrophoresis, gel permeation chromatography, Fourier transform-infrared (FT-IR) spectroscopy, intrinsic fluorescence (IF) spectroscopy, and circular dichroism (CD) spectroscopy were applied. According to the findings, both CTS and COS dramatically reduced intestinal digestibility of WPI, resulting in a decrease of DH by 43.33 % and 52.31 %, respectively. The substitution degree of WPI on CTS was 0.87 g WPI/g CTS, and the electrostatic interaction between amine groups of CTS and carboxyl groups of WPI caused changes in WPI's stability, microstructure, and fluorescence intensity. Notably, CTS affected the digestibility of WPI by precipitating protein and enzyme, whereas COS altered WPI's digestibility by decreasing or inactivating enzyme activity. The present study offered a solid scientific foundation for the rational formulations of carbohydrates and proteins in food industry.
研究领域:
食品科学
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营养学
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生物物理学
·
化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2018
·Vol. 42
·期 6
·e12658
·
IF 1.552
Yuhong Liu,Zhimin Sun,Lili Xiu,Jianying Huang,Fengyan Zhou
Two fungal isolates, F12-20 and F3-1, were collected from postharvest blueberry in China. By phylogenetic analysis with the nuclear ribosomal internal transcribed spacer region and morphological analyses, they were initially identified as Penicillium and Arthrinium sacchari ( A. sacchari ). The results showed that water-soluble chitosan (WCS) and sulfonated chitosan (SCS) could significantly reduce the radial growth of fungal mycelia. The best concentrations were 0.25 and 16 g L –1 for WCS and 0.50 and 64 g L –1 for SCS against Penicillium sp. and A. sacchari , respectively. Importantly, SCS exhibited selective antifungal activity against Botrytis cinerea and A. sacchari . As different species of fungi may respond differently to fungicides, the findings of this study will provide a basis for the development of target-specific pathogen control and will be useful in designing and implementing fungicide resistance management for control postharvest decay, thus extending the storage life of fresh blueberry. Practical application Experimental results obtained herein are useful for designing and implementing fungicide resistance management for control postharvest decay in blueberry to improve marketable fruit and extend the storage life of fresh blueberries. And sulfonated chitosan can be applied to the target-specific pathogen control.
研究领域:
食品科学
·
植物病理学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Lin Li,Ning Wang,Chang Liu,Liqi Wang,Na Zhang,Yingjie Yu,Dianyu Yu
Soybean oil body (SOB) emulsion was combined with carboxymethyl chitosan (CMCS) to improve the stability of the oil body system, and the influence on the characteristics of SOB emulsion was explored. It was found that the particle size of SOB emulsion without CMCS was the smallest, 422.7 nm. After the addition of CMCS, the particle size of all samples increased, and the particle size of SOB-CMCS emulsion was the smallest (480.0 nm) when the amount of CMCS was 0.4 %. At this time, the surface hydrophobicity (H 0 ) of the sample is 230.5, and the emulsification stability is 69.83 min. The results of interfacial stability show that adding appropriate concentration of CMCS can reduce the interfacial tension of the sample. The results of rheological model fitting showed that the consistency coefficient of SOB-CMCS samples with 0.8 % CMCS addition was 3.75, which was the highest among all groups. The environmental stability test of SOB-CMCS emulsion showed that the particle size of SOB-CMCS emulsion with 0.4 % CMCS content was 944.0 nm after heating at 90 °C for 30 min, and the particle size of SOB-CMCS emulsion was 979.8 nm at 300 mmol/L salt ion concentration, which showed the smallest change compared with other groups.
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Lin Li,Peng Zhang,Congcong Li,Yan Guo,Kaoxiang Sun
Major obstacles in the development of nanoformulations as efficient drug delivery systems are the rapid clearance from blood circulation and lysosomal entrapment. To overcome these problems, a polysaccharide-based core-shell type charge-switchable nanoformulation (CS-LA-DMMA/CMCS/ [email protected] ) is constructed to improve antitumor efficacy of DOX. By applying carboxymethyl chitosan (CMCS) as bridge polymer and negatively charged chitosan-derivative as outer shell, the stability and pH-sensitivity of this nanoformulation is promisingly enhanced. Furthermore, the positively charged [email protected] could escape from lysosomes via “proton sponge effect” and “cationic–anionic interaction with lysosome membranes”. Admirable cellular uptake and high apoptosis/necrosis rate were detected in this study. In vitro assays demonstrate that the CS-LA-DMMA/CMCS/ [email protected] was internalized into HepG2 cells predominantly via the clathrin-mediated endocytosis pathway. Excitingly, in vivo studies showed that high accumulation of CS-LA-DMMA/CMCS/ [email protected] in tumor tissue led to enhanced tumor inhibition. Compared with free DOX, the tumor inhibition rate of nanoformulation was improved up to 226%.
研究领域:
药学
·
纳米技术
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Qun Liu,Yan Li,Xiaodeng Yang,Shu Xing,Congde Qiao,Shoujuan Wang,Chunlin Xu,Tianduo Li
Chitosan has attracted much attention in drug delivery, however, carboxymethyl chitosan (CMC)-based self-aggregated nanocarriers are seldom reported. In this paper, two kinds of CMC-based pH-responsive amphiphilic chitosan derivatives, N -2-hydroxylpropyl-3-butyl ether- O -carboxymethyl chitosan (HBCC) and N -2-hydroxylpropyl-3-(2-ethylhexyl glycidyl ether)- O -carboxymethyl chitosan (H2ECC), have been synthesized by the homogeneous reaction. The molecular structures were characterized by FTIR, 1 H NMR and 13 C NMR. The optimum reaction condition was obtained based on the data of 1 H NMR spectrum: reaction time of 4 h, reaction temperature of 80 °C and n e p o x y n - N H 2 of 3/1, respectively. The XRD patterns showed the crystallinity of HBCC and H2ECC decreased due to the introduction of hydrophobic segments. The thermostability of HBCC and H2ECC was improved for the formation of heat-resistant stereo-complexed structures. The intermolecular hydrophobic interaction hindered the intermolecular mobility by increasing glass transition temperature of ca. 10 °C. Both HBCC and H2ECC have very low critical aggregation concentrations (HBCC: 0.66–1.56 g/L, H2ECC: 0.57–1.07 g/L) and moderate aggregate particle size, which is advantageous for utilization as a drug carrier. The curcumin loaded HBCC and H2ECC aggregates showed nontoxicity, meanwhile, HBCC and H2ECC showed good antibacterial activity against Staphylococcus aureus and Escherichia coli . As a result of these two favorable properties, HBCC and H2ECC could be used as curcumin nanocarriers as well as antibacterial agents.
研究领域:
生物材料学
·
药剂学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Yanzhen Zhao,Liu Yang,Meirong Xu,Huifang Wang,Xianghua Gao,Baolong Niu,Wenfeng Li
Food spoilage caused by bacterial growth is a serious threat to human health. Silver nanoparticles (AgNPs) have antibacterial activity against various pathogenic microorganisms, but their rapid release often leads to cumulative toxicity. In this paper, silver nanoparticles (AgNPs@CG) were reduced and immobilized in situ using gallic acid-functionalized chitosan (CG) as a reducing and stabilizing agent to achieve a long-lasting and stable controlled release of Ag + . The AgNPs@CG was incorporated into the CG/PVA composite film. The results showed that the release of Ag + was only 0.686 ± 0.022 mg·L −1 after seven days, which had a long-lasting antibacterial effect on E. coli and S. aureus . In addition, CG/PVA/AgNPs-2 composite film can significantly improve the freshness preservation effect in fresh-cut apple preservation applications. In conclusion, CG/PVA/AgNPs composite film has potential applications as effective and safe packaging material to extend the shelf life of food products.
研究领域:
食品科学
·
材料科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Tao Liu,Shuhan Liu,Yihan Shi,Zhuoran Zhang,Sheng Ding,Kexin Hou,Wen Zhang,Xin Meng,Fan Li
The ideal hemostatic agents should be able to stop bleeding quickly and avoid secondary bleeding caused by adhesion with blood clots during dressing change. Herein, a hydrophobic electrospun nanofiber membrane was prepared for achieving hemostasis , rationally targeting both attributes, via doping N -alkylated chitosan ( N -CS) grafted with octadecyl into chitosan/polyethylene oxide (PEO). In vitro and in vivo coagulation tests showed that CPNs doped with small amounts of N -CS (CPN31) could significantly shorten hemostasis time and promote the formation of more stable and stronger blood clots. In particular, the whole blood clotting time of CPN31 (58.8 ± 2.2 s) was significantly lower than that of chitosan/PEO (CPN0) nanofiber membrane (67 ± 3.5 s) and the medical sterile gauze (86.7 ± 0.6 s). Furthermore, due to the hemophobic nature of CPNs, blood wetting of the dressing was severely limited and blood can coagulated at the site of liver injury in rats, thus reducing blood loss and allowing rapid removal of the dressing without triggering secondary hemorrhage. The CPN31 exhibited excellent hemostasis properties, easy to remove, blood compatibility, biocompatibility and promoting fibroblast proliferation properties. This hydrophobic CPNs is a promising biological adhesive for hemorrhage control.
研究领域:
生物医学工程
·
材料科学
·
医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）