INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Jing Zhou,Rourou Wang,Ming Niu,Zhenghua Zhang,Minrui Zhu,Lumei Pu,Weibing Xu,Runtian Ma
The infection caused by Fusarium oxysporum severely threatens cotton production. Herein, carvacrol-chitosan nanoparticles (CS@CAR) are facilely fabricated using tripolyphosphate as a crosslinker. When the initial mass ratio of chitosan to carvacrol is 1:0.4, the nanoparticles show the best stability and drug loading. Morphological analysis reveals that the CS@CAR nanoparticles possesses a uniform spherical structure with an average diameter of approximately 150 nm. About 51.23 % of total CAR is released within the 48 h in the simulated fungal infection environment. The contact angle of drug-loaded particles decreases by 12.2 and 6.6 ° compares with that of pure water and unloaded particles. The fluorescence intensity of leaves before and after rain erosion is 1.3 times and 1.2 times that of the pure water control group by fluorescence imaging analysis. The bacteriostatic activity of drug-loaded nanoparticles is 6–7 times that of non-drug-loaded nanoparticles. The contents of nucleic acid, protein, and malondialdehyde leaked by spores after treatment with drug-loaded particles are 1.4, 1.5 and 2.3 times those in the non-drug-loaded particle treatment group, respectively. The CS@CAR entirely prevents Fusarium oxysporum from infecting cotton leaves in spore inoculation experiments. The CS@CAR nanoparticles are expected to be used to prevent and control the infection of Fusarium oxysporum on cotton.
研究领域:
植物病理学
·
纳米生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Junhua Lu,Xinyi Yin,Qiu Wu,Mengjiao Wang,Liying Han,Shupeng Su
The fabrication of Pickering nanoemulsions, specifically food-grade ones, is challenging due to the limited availability of particles with appropriate amphiphilic wettability and sufficiently small particle size. Chitosan nanoparticles offer a promising solution due to their adjustable size and amphiphilic properties. The study investigated the effects of pH, oil phase volume fraction, chitosan concentration, and ultrasonic intensity on the formation of chitosan Pickering nanoemulsions. Nanoemulsions with droplet size of approximately 300 nm were achieved under optimal conditions of pH 6.5, oil phase volume fraction of 1 %, chitosan concentration of 1.0 wt%, and 455 W ultrasonic power for 360 min. Stability tests indicated that the nanoemulsions had good pH (2–6), ionic strength (100–500 mM), thermal (60–90 °C), freeze-thaw (3 times) and storage stabilities (0–60 days). The findings highlight the potential of chitosan nanoparticles as effective stabilizers for Pickering nanoemulsions, providing valuable insights for the development of food-grade Pickering nanoemulsions.
研究领域:
食品科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2020
·Vol. 18
·期 6
·315
·
IF 4.073
Qinying Yan,Jiaqi Weng,Xieqi Wu,Weiwei Wang,Qingliang Yang,Fangyuan Guo,Danjun Wu,Ying Song,Fan Chen,Gensheng Yang
Chitosan nanoparticles (CS-NPs) are under increasing investigation for the delivery of therapeutic proteins, such as vaccines, interferons, and biologics. A large number of studies have been taken on the characteristics of CS-NPs, and very few of these studies have focused on the microstructure of protein-loaded NPs. In this study, we prepared the CS-NPs by an ionic gelation method, and bovine serum albumin (BSA) was used as a model protein. Dynamic high pressure microfluidization (DHPM) was utilized to post-treat the nanoparticles so as to improve the uniformity, repeatability and controllability. The BSA-loaded NPs were then characterized for particle size,Zetapotential, morphology, encapsulation efficiency (EE), loading capacity (LC), and subsequent release kinetics. To improve the long-term stability of NPs, trehalose, glucose, sucrose, and mannitol were selected respectively to investigate the performance as a cryoprotectant. Furthermore, trehalose was used to obtain re-dispersible lyophilized NPs that can significantly reduce the dosage of cryoprotectants. Multiple spectroscopic techniques were used to characterize BSA-loaded NPs, in order to explain the release process of the NPs in vitro. The experimental results indicated that CS and Tripolyphosphate pentasodium (TPP) spontaneously formed the basic skeleton of the NPs through electrostatic interactions. BSA was incorporated in the basic skeleton, adsorbed on the surface of the NPs (some of which were inlaid on the NPs), without any change in structure and function. The release profiles of the NPs showed high consistency with the multispectral results.Keywords:characteristics;cryoprotection evaluation;dynamic high pressure microfluidization;chitosan nanoparticles
研究领域:
生物材料学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Bohui Xu,Wenjing Zhang,Yulin Chen,Yan Xu,Bo Wang,Li Zong
The aim of this study was to develop a novel biodegradable polymeric carrier for the delivery of protein vaccine orally in order to target the antigen presenting cells (APCs) in the region of Peyer's patches (PPs). Here, bovine serum albumin (BSA) was chosen as a model protein vaccine and was loaded into the mannosylated chitosan nanoparticles (MCS NPs) by ionic gelation method with tripolyphosphate (TPP), followed by coating MCS NPs with Eudragit® L100 (Eud) by electrostatic interaction. The spherical NPs were successfully prepared with appropriate particle size around 558.2 ± 35.6 nm, high entrapment efficiency about 90.38 ± 9.12%, good stability and reasonable release behavior in the simulated gastrointestinal fluid, meanwhile high resistance of enzymatic and acid degradations were verified. The targeting ability of the NPs to PPs in rat was investigated by a closed ileal loop assay, where fluorescence visualization was performed. MCS NPs were accumulated more specifically into PPs after Eudragit® L100 dissolved in intestinal juices. Oral immunization using BSA-loaded Eudragit® L100-coated MCS NPs was found to elicit strong systemic IgG antibody and mucosal IgA responses. These results suggested that enteric-coated MCS NPs could serve as a promising carrier for oral protein vaccine delivery.
研究领域:
药剂学
·
免疫学
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纳米技术
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2021
·
IF 2.104
Jun Wang,Zongyong Zhang,Yilong Ai,Fang Liu,Min-Min Chen,Dahai Liu
In order to achieve efficient delivery of methotrexate (MTX), thymine-chitosan nanoparticles (Thy-Cs NPs) were prepared, and further decorated with lactobionic acid (LA) to obtain tumor-targeting nanoparticles (LA-Thy-Cs NPs). These nanoparticles possessed a regular spherical structure with the average size about 190–250 nm and narrow size distribution, which were kinetically stable in the physiological environment. Due to electrostatic interactions and multiple hydrogen-bonding interactions between MTX and carriers, MTX was loaded into Thy-Cs NPs with high drug loading content (~20%). MTX release from Thy-Cs NPs was significantly accelerated in the mildly acidic environment due to the destruction of two types of non-covalent interactions. In vitro cell experiments demonstrated that LA-Thy-Cs NPs could be efficiently internalized into hepatoma carcinoma cells, leading to higher cytotoxicity. Moreover, MTX-loaded LA-Thy-Cs NPs performed an enhanced growth inhibition in three-dimensional multicellular tumor spheroids . Thus, the LA decorated thymine-chitosan nanocarriers can be a promising candidate for efficient delivery of MTX.
研究领域:
药学
·
纳米技术
·
癌症研究
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2015
·Vol. 5
·期 3
·1379-1396
·
IF 2.076
Li Zhang,Tingxian Liu,Yanan Xiao,Dexin Yu,Na Zhang
Molecular imaging is essential to increase the sensitivity and selectivity of cancer diagnosis especially at the early stage of tumors. Recently, polyionic nanocomplexes (PICs), which are composed of polyanions and opposite polycations, have been demonstrated to be a promising strategy for biomedical applications. In this work, chitosan-hyaluronic acid nanoparticles (GCHN) were developed to deliver Gd-DTPA as MRI contrast agents for tumor diagnosis. The Gd-labeled conjugates (CS-DTPA-Gd) were successfully synthesized by carbodiimide reaction, and then GCHN were prepared by ionic gelation using the obtained CS-DTPA-Gd and hyaluronic acid. The morphology of GCHN was spherical or ellipsoidal, which is observed by transmission electronic microscopy (TEM). The mean particle size and zeta potential of GCHN were 213.8 ± 2.6 nm and 19.92 ± 1.69 mV, respectively. The significant enhancement of signal intensity induced by GCHN was observed bothin vitroandin vivo. Also, compared with Magnevist, GCHN was witnessed for a prolonged imaging time in the B16 tumor-bearing mice model. Furthermore, GCHN were verified as below toxic bothin vitroandin vivo. These results indicated that GCHN could potentially be an alternative to current MRI contrast agents for tumor diagnosis.Keywords:tumor imaging;magnetic resonance imaging;hyaluronic acid;chitosan nanoparticles;Gd-DTPA
研究领域:
医学影像学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2018
·
IF 0
Yuangang Liu,Weiji Weng,Shibin Wang,Ruimin Long,Hanwen Li,Huihui Li,Tengteng Li,Mengyi Wu
Diabetes mellitus is the most common endocrine disease worldwide; hyperglycemia is a hallmark of this disease. To alleviate the pain caused by diabetes, developing and utilizing effective diabetic drugs to maintain or recover the function of the residual β-cells is an attractive therapeutic approach. γ-aminobutyric acid (GABA) has been shown to have such effects, but it is easy to have reduced GABA activity under physiological conditions. In the present study, GABA–chitosan nanoparticles (GABA–CS NPs) were prepared, and glucose homeostasis, pancreatic β-cell protection, and anti-inflammatory effects of GABA–CS NPs were investigated in vivo. The results showed that blood glucose levels and IL-1β levels in the GABA–CS NP-administered group were both significantly lower, whereas the PDX1 expression was significantly higher than that of the impaired group (p < 0.01). This indicates that GABA–CS NPs can efficiently maintain glucose homeostasis, protect β-cells, and inhibit inflammation. These nanoparticles have the potential to be applied for future diabetes theranostics.
研究领域:
药理学
·
内分泌学
·
纳米技术
·
糖尿病研究
✓ 已验证
DOI
PubMed
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Yuan-Fu Ding,Shengke Li,Lijun Liang,Qiaoxian Huang,Lihui Yuwen,Wenjing Yang,Ruibing Wang,Lian-Hui Wang
The photosensitizer Chlorin e6 (Ce6) has been frequently employed for photodynamic therapy (PDT) of cancer; however, its nonspecific toxicity has limited its clinical applications. In this study, we prepared chitosan nanoparticles (CNPs), with a mean diameter of approximately 130 nm, by a nonsolvent-aided counterion complexation method in an aqueous solution, into which Ce6 could be physically entrapped during the preparation process. These CNPs and Ce6-loaded CNPs (CNPs-Ce6) were fully characterized by UV–vis, photoluminescence, and Fourier transform infrared spectroscopic analysis, as well as dynamic light scattering and transmission electron microscopy measurements. More importantly, the biocompatibility of the otherwise toxic Ce6 was significantly improved upon its loading into the CNPs, as demonstrated by both confocal laser scanning microscopy analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Furthermore, the PDT efficiency of Ce6-loaded CNPs was dramatically enhanced, in comparison with that of the free Ce6, as shown by both MTT and flow cytometry assays. This discovery provides a novel strategy for improving the biocompatibility and therapeutic efficacy of PDT agents by using a natural, biocompatible polysaccharide carrier.
研究领域:
肿瘤学
·
药剂学
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2023
·Vol. 25
·期 37
·25780-25788
·
IF 3.3
Runna He,Shuangqing Sun,Jianpeng Cui,Mingshuo Chi,Zhikun Wang,Songqing Hu
In recent years, Pickering emulsifiers have been widely used in various production fields due to their excellent structural stability, biocompatibility and environmental friendliness. For some applications, it is required that the emulsifier can quickly respond to environmental stimuli and control the transition between stable and unstable emulsions. In this paper, we report a novel composite Pickering emulsifier with Fe3O4 as the core and magnetic response recognition body, silica as the intermediate protective layer, and chitosan (CS) of different molecular weights to endow solid particles with surface activity and pH-responsive properties. This emulsifier can stabilize the emulsion in the emulsion system with deionized water as the aqueous phase and liquid paraffin as the oil phase and can control the demulsification of the formed emulsion under the dual pH/magnetic stimulation. The experimental results show that Fe3O4@SiO2@CS has good paramagnetism and pH responsiveness. The particle size of the composite emulsifier nanoparticles is between 90 nm and 120 nm, and the best stabilizing effect of the emulsion is achieved when the dosage is 0.5 wt%. In the pH range of 3–11, the emulsifier can rapidly demulsify a stable paraffin oil–water emulsion system under the action of a magnetic field of strength 0.4 T. The pH response of the emulsifier is as follows: when pH ≤ 2, the system can form a stable emulsion, which is composed of fully protonated chitosan as a free chain segment and Fe3O4@SiO2. Emulsion stabilization was achieved with monolithic Fe3O4@SiO2@CS as an emulsifier at pH > 2, and demulsification was achieved at pH ≈ pKb (CS) at 298 K. The research in this paper can provide a feasible idea and synthesis method for the preparation of organic–inorganic composite structure emulsifier.
研究领域:
材料科学
·
化学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CURRENT MOLECULAR MEDICINE
·
2018
·Vol. 18
·期 6
·383-391
·
IF 2.254
Li Yue,Lin Yan,Liufu Chun,Tu Jiawei,Yang Chaopin,Chen ZhiYi
Objective: To study transfection efficiency of folate-modified chitosan (FACS) nanoparticles as a non-viral vector delivering pEGFP-C3plasmid (FA-CS/P) to 293T cells with or without the combination of ultrasound and microbubble. Method: pEGFP-C3 was used as a reporter gene and
FA-CS nanoparticles, which were prepared by complex coagulation method, were used as biological carriers. Transfection efficiency to 293T cells mediated by FA-CS/P nanoparticles, ultrasound (US) and microbubble (MB) was assessed by fluorescence microscopy and flow cytometry. Result: FA-CS/P
nanoparticles have a particle size of 355.1 nm and zeta potential of 10.4 mV. Significant green fluorescence could be observed in CS/P group, FA-CS/P group, US+MB/P group, US+FA-CS/P group, Liposome 2000 (L) group under an inverted fluorescence microscope, while for US+MB+FA-CS/P group, only
scattered fluorescence was observed. Result of flow cytometry showed that the transfection rate of US+MB+FA-CS/P group was (2.0±0.2)%, which was significantly lower than other groups (P<0.05). CCK-8 experiments showed that cell vitality of US+MB+FA-CS/P was groups (P<0.05). CCK-8
experiments showed that cell vitality of US+MB+FA-CS/P was (64.1±4.6)%, which was also lower than other groups (P<0.05). Conclusion: In this study, FA-CS was successfully synthesized. FA-CS could combine with pEGFP-C3 effectively forming nanoparticles with nanoparticle size,
well dispersion, high encapsulation efficiency and no significant toxicity to cells. The application of ultrasound increased the transfection rate of FA-CS/P. However, while being exposed to ultrasound and microbubble, the transfection rate of FA-CS/P decreased obviously, may indicate that
there was no synergistic effect for gene transfection by the combination of ultrasound, folate modified chitosan and microbubbles.
研究领域:
生物医学工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiayao Yang,Jie Fei,Xiaohuan Liu,Hongpeng Wang,Jun Huang
The advancement of biomedicine and molecular diagnostics depends on effective nucleic acid separation methods. Nevertheless, creating high-performing and environmentally friendly nucleic acid adsorbent materials is still challenging. In order to achieve high-quality DNA capture, this work creatively created a rare earth metal hybridized magnetic chitosan nanoparticle (Fe 3 O₄@CS@La(OH)₃) by combining the biocompatibility of chitosan with the effective coordination ability of La 3+ . The results showed that Fe₃O₄@CS@La(OH)₃ had a high specific surface area and superparamagnetism, with a high adsorption capacity of DNA up to 370.37 mg/g, and 80 % of DNA could be captured within 5 min, and the capture rate was up to 96.7 % within 2 h. This was primarily ascribed to the strong coordination and electrostatic interaction between La 3+ and nucleic acids. Furthermore, the adsorption efficiency continued to exceed 90 % after five cycles, indicating exceptional reproducibility. This study involved the novel combination of the rare earth metal lanthanum with magnetic chitosan. The utilization of its unique coordination chemistry significantly enhanced the capture efficiency of nucleic acids, presenting potential applications in precision medicine and disease detection.
研究领域:
生物医学工程
·
纳米技术
·
分子诊断学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2024
·
IF 3.6
Xinyu Yang,Dongdong Meng,Ning Jiang,Chaoxing Wang,Jinbo Zhang,Yanqiu Hu,Jiaming Lun,Rui Jia,Xueyun Zhang,Weitong Sun
In this study, the pH-responsive API-CMCS-SA (ACS) polymeric nanoparticles (NPs) based on 1-(3-amino-propyl) imidazole (API), stearic acid (SA), and carboxymethyl chitosan (CMCS) were fabricated for the effective transport of curcumin (CUR) in liver cancer. CUR-ACS-NPs with various degrees of substitution (DS) were employed to prepare through ultrasonic dispersion method. The effect of different DS on NPs formation was discussed. The obtained CUR-ACS-NPs (DSSA=12.4%) had high encapsulation rate (more than 85%) and uniform particle size (186.2 ± 1.42 nm). The CUR-ACS-NPs showed better stability than the other groups. Drug release from the CUR-ACS-NPs was pH-dependent, and more than 90% or 65% of CUR was released in 48 h in weakly acid medium (pH 5.0 or 6.0, respectively). Additionally, the CUR-ACS-NPs increased the intracellular accumulation of CUR and demonstrated high anticancer effect on HepG2 cells compared with the other groups. CUR-ACS-NPs prolonged the retention time of the drug, and the area under the curve (AUC) increased significantly in vivo. The in vivo antitumor study further revealed that the CUR-ACS-NPs exhibited the capability of inhibiting tumor growth and lower systemic toxicity. Meanwhile, CUR, CUR-CS-NPs, and CUR-ACS-NPs could be detected in the evaluated organs, including tumor, liver, spleen, lung, heart, and kidney in distribution studies. Among them, CUR-ACS-NPs reached the maximum concentration at the tumor site, indicating the tumor-targeting properties. In short, the results suggested that CUR-ACS-NPs could act a prospective drug transport system for effective delivery of CUR in cancer treatment.
研究领域:
药学
·
癌症研究
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）