SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Ting Yin,Sheng Zhang,Mengxuan Li,Carl Redshaw,Xin-Long Ni
Supramolecular p K a shifts, have attracted much attention in catalytic and biomimetic studies because of their excellent property to modify the acidity or basicity of the substrate in aqueous media by host-guest inclusion. Here, Fluorescence indicator displacement based on cucurbit[8]uril encapsulation of the dye acridine leads to the recognition of the peptide drug octreotide in aqueous solution via distinctive pH signals was expolited. This is thought to be a result of competitive host-guest interactions involving a supramolecular p K a shift.
研究领域:
生物化学
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药物化学
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分析化学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Yong-Qi Cai,Gao Li
Many fluorometric methods have been developed for detecting tyrosinase activity, yet many of them require the sophisticated synthesis of relatively complex fluorescent molecules. Here, we report the utilization of a label-free supramolecular tandem assay for measuring tyrosinase activity based on an easily available reporter pair. Cucurbit[8]uril (CB[8]) can bind with thioflavin-T (ThT) and increase its fluorescence intensity , while the tripeptides YLA and Dopa-LA can decrease the fluorescence intensity of CB[8]-ThT complex. YLA and Dopa-LA can be oxidized by tyrosinase, and the products have lower binding affinity with CB[8], leading to an increase of fluorescence in the intensity of CB[8]-ThT-YLA or Dopa-LA. We were able to continuously monitor tyrosinase activity with CB[8]-ThT-YLA or Dopa-LA.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2023
·
IF 3.8
Pei-Hui Shan,Ding-Wu Pan,Li-Xia Chen,Timothy J. Prior,Carl Redshaw,Zhu Tao,Xin Xiao
Herein, we report a simple fluorescence-enhanced system for the selective recognition and determination of the insecticide pymetrozine. 1 H NMR spectroscopic data indicate that 1,2-bis(4-pyridyl)ethylene (BPE) is partially encapsulated in the cavity of the cucurbit[8]uril (Q[8]) in aqueous solution, forming a stable 1:2 host-guest inclusion complex. Good evidence is also provided by other characterization techniques including single crystal X-ray diffraction, UV–Vis and fluorescence spectroscopies. This host-guest inclusion complex shows weak fluorescence in aqueous solution. Interestingly, the addition of pymetrozine greatly enhanced the fluorescence of the host-guest inclusion complex. In contrast, no significant fluorescence enhancement was observed on addition of 10 other pesticides. The concentration of pymetrozine in aqueous solution was easily detected based on the linear relationship between fluorescence intensity and pymetrozine concentration. Therefore, this paper reports a new method to identify and determine pymetrozine by fluorescence enhancement.
研究领域:
分析化学
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农药科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Qinye Liu,Xueting Sun,Yuanpeng Wang,Yueyue Chen
Tumor metastasis is a difficult clinical problem to solve due to tumor heterogeneity and the emergence of antiapoptotic clones driven by tumor evolution. Clinical combination chemotherapy remains a standard treatment for solid metastasis tumors but with worse treatment efficiency. It is worth exploring a high-efficiency and low-side-effect therapeutic method to solve solid metastases. Herein, we fabricate a supramolecular combination chemotherapeutic system based on host–guest interactions using a cucurbit[8]uril complex (CB[8]–lobaplatin–oxaliplatin, CLO). We explored whether CLO can induce colorectal cancer cell death by activating immunogenic cell death (ICD) to inhibit cancer cell migration in a safe and effective manner. CLO inhibits the viability of human colorectal cancer cells (HCT116) at only 10 μM and alleviates the cytotoxicity induced by lobaplatin–oxaliplatin (LO) in normal colorectal cells (NCM460). Interestingly, CLO can trigger ICD in colorectal tumor cells, and our results verified the upregulations of high mobility group box 1 (HMGB1) and adenosine triphosphate (ATP) release and cell surface calreticulin (CRT) exposure. Besides, we found that CLO can inhibit tumor migration both in vivo and in vitro, and bio-AFM characterization of tumor cellular surface also confirmed this phenomenon. To further investigate the underlying mechanisms of the antitumor effects of CLO, quantitative proteomics was employed, and the results indicated that the citrate cycle (TCA cycle), protein processing in the endoplasmic reticulum, cGMP-PKG signaling pathway, p53 signaling pathway, chemical carcinogenesis, and necroptosis signaling pathway might contribute to CLO-induced antitumor effects. Collectively, our study suggests that the supramolecular combination chemotherapeutic system based on CB[8] host–guest complex can activate ICD to inhibit metastasis in antitumor strategy and exhibits a remarkable potential for supramolecular immunotherapy.
研究领域:
肿瘤学
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药理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL RESEARCH
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2019
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IF 0.67
Chunchun Chen,Weiwei Ge,Kai Chen,Zhu Tao,Yunqian Zhang,Qian Jiang Zhu
Cucurbit[8]uril (Q[8]) was used as the building block for the facile preparation of Q[8]-based supramolecular assemblies in aqueous HCl solutions (A). The Q[8]-based supramolecular assembly was constructed of Q[8] molecules held together through outer surface interactions of cucurbit[n]urils, and hydrogen bonding between latticed water molecules and portal carbonyl oxygens of Q[8]. The porous structure resulted in different adsorption properties for different fluorophore guests (FGs), including dyes and fused-ring compounds, and fluorescence enhancement or colour changes resulted in luminescent materials (FG@As). Moreover, some of the FG@A systems could selectively respond to certain volatile organic compounds, for which they can be used as solid sensors.
研究领域:
化学
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材料科学
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分析化学
✓ 已验证
DOI
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
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2026
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IF 3
Yinghui Niu,Fengbiao Chen,Xinru Guo,Yinjun Chen,Jin Wen,Meifang Zhu
Spiropyrans (SPs) are promising building blocks for stimuli-responsive delivery because of their sensitivity to pH, light, and heat. However, their intrinsic hydrophobicity and limited stability in aqueous media severely restrict practical use under physiological conditions. Here, to overcome this aqueous-compatibility bottleneck, we propose a host-guest-guided design and screening strategy that integrates thermodynamic binding analysis with kinetic assessment, followed by experimental validation, to identify effective supramolecular complexes for controlled loading/release in water. Using cucurbit[8]uril (CB[8]) and three spiropyran derivatives (SP1–3) with their merocyanine isomers (MC1–3) as a model system, we first quantify host-guest recognition from a thermodynamic perspective by density functional theory. We then resolve stimulus-coupled loading/release kinetics by explicit-solvent umbrella sampling, yielding potential of mean force profiles that reveal a pronounced size-dependent kinetic-gating mechanism: MC1–2 display an entry–retention behavior consistent with efficient loading and high kinetic retention, while the bulkier SP3/MC3 is sterically restricted and favors a shallow capping mode with limited encapsulation and faster escape. ¹H NMR and time-resolved UV-Vis measurements further verify the strong host-guest interactions and the host-modulated isomerization kinetics, in close agreement with the differentiated confinement regimes predicted by simulation. Beyond CB[8], comparative simulations indicate that γ-cyclodextrin provides a more accommodating cavity environment for SP2. Overall, this work demonstrates a generalizable theory-to-experiment workflow that couples thermodynamic and kinetic criteria to rationally select host-guest pairs, offering a transferable framework for designing low-leakage, multi-stimuli-responsive supramolecular delivery and controlled-release platforms.
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Weitao Xu,Huaming Feng,Weiwei Zhao,Chunhua Huang,Carl Redshaw,Zhu Tao,Xin Xiao
A new fluorescent chemosensor comprised of cucurbit[8]uril (Q[8]) and acridine hydrochloride (AC) has been designed and utilized for the recognition of amino acids . The AC was encapsulated by the Q[8] cavity and formed a 1:2 host-guest inclusion complex both in solution (aqueous) and in the solid-state. Whilst free AC is known to be strongly fluorescent, this strong fluorescence was quenched in the inclusion complex Q [8]-AC. This non-fluorescent complex Q[8]-AC was capable of serving as a fluorescence “off-on” probe, and was able to recognize either L -Phe or L -Trp via the competitive interaction between L -Phe or L -Trp. Moreover, the pH responsive nature of the probe allowed for the detection of basic amino acids, namely L -Arg, L -His, or L -Lys). As a result, a fluorescence method for the detection of five amino acids using a single system has been developed.
研究领域:
化学
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分析化学
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2018
·Vol. 14
·期 1
·235-242
·
IF 3.692
Weitao Xu,Jinglan Kan,Bo Yang,Timothy J. Prior,Bing Bian,Xin Xiao,Zhu Tao,Carl Redshaw
Graphical Can I have some pumpkin soup, please? In the host–guest complexes formed between cucurbit[8]uril and the alkyl-substituted 4-pyrrolidinopyridinium salts 4-(C 4 H 8 N)C 5 H 5 NRBr (R=Et, n -butyl, n -pentyl, n -dodecyl), the orientation of the guest molecule changes with the length of R. Solid state structures are different to those observed in solution, and for R= n -pentyl, there are two different interactions between Q[8] and the guest. The interaction between cucuribit[8]uril (Q[8]) and a series of 4-pyrrolidinopyridinium salts bearing aliphatic substituents at the pyridinium nitrogen, namely 4-(C 4 H 8 N)C 5 H 5 NRBr, where R=Et (g1), n -butyl (g2), n -pentyl (g3), n -hexyl (g4), n -octyl (g5), n -dodecyl (g6), has been studied in aqueous solution by 1 H NMR spectroscopy, electronic absorption spectroscopy, isothermal titration calorimetry and mass spectrometry. Single crystal X-ray diffraction revealed the structure of the host–guest complexes for g1, g2, g3, and g5. In each case, the Q[8] contains two guest molecules in a centrosymmetric dimer. The orientation of the guest molecule changes as the alkyl chain increases in length. Interestingly, in the solid state, the inclusion complexes identified are different from those observed in solution, and furthermore, in the case of g3, Q[8] exhibits two different interactions with the guest. In solution, the length of the alkyl chain plays a significant role in determining the type of host–guest interaction present.
研究领域:
化学
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超分子化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2014
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IF 4.258
Tao Sun,Ningning Ji,Meiling Qi,Zhu Tao,Ruonong Fu
Here we report the investigation of using cucurbit[8]uril (CB8) and its coordination complex with cadmium (II) (CB8-Cd) as stationary phases for capillary gas chromatography (GC). The prepared capillary columns of CB8 and CB8-Cd stationary phases achieved column efficiency of 2200 plates/m and 1508 plates/m, respectively, and showed weak polarity based on the measured McReynolds constants. Their separation performance was investigated by GC separation of mixtures of different types while a commercial column was used for comparison. The CB8 stationary phase achieved high resolution for a wide range of analytes from nonpolar to polar while the CB8-Cd stationary phase exhibited good separation mainly for nonpolar to weak polar analytes. The CB stationary phases differ from the commercial one in terms of retention behaviors and resolving ability due to their different molecular interactions with analytes. Moreover, energy effect on the retention of analytes on CB8 and CB8-Cd stationary phases was examined, showing that retention on CB8 column was determined mainly by enthalpy change for polar analytes and by both enthalpy change and entropy change for weak polar analytes whereas retention on CB8-Cd column was mainly controlled by entropy change. This work demonstrates the great potential of CB8 and CB8-Cd stationary phases as a new type of GC stationary phases in GC analysis.
研究领域:
分析化学
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Qianchun Zhang,Gongke Li,Xiaohua Xiao,Song Zhan,Yujuan Cao
Cytokinins play a critical role in controlling plant growth and development, but it is difficult to be determined in plant samples due to the extremely low concentration level of picomole/gram. So it is important for efficient sample preparation with selective enrichment and rapid separation for accurate analysis of cytokinins. Herein, a supramolecular perhydroxy-cucurbit[8]uril (PCB[8]) was fabricated into the Fe3O4 magnetic particles via chemical bonding assembly and magnetic perhydroxy-cucurbit[8]uril (MPC) materials were obtained. The MPC had good enrichment capability to cytokinins and the enrichment factors were more than 208. The interaction of MPC and cytokinins was investigated by adsorption test and density functional theory (DFT) calculation, the results showed that the main drive forces were the host–guest interaction and hydrogen-bonding interaction between the perhydroxy-cucurbit[8]uril with analytes. Combined with ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS), the MPC was used as a sorbent of magnetic solid-phase extraction for the analysis of cytokinins in plant samples. A sensitive and selective UPLC–MS/MS method was developed with low detection limits of 0.14–0.32 ng/L for cytokinins analysis. Five cytokinins including zeatin riboside, meta-topolin, kinetin, kinetin riboside, and zip with 6.12–87.3 ng/kg were determined in the soybean sprout and Arabidopsis thaliana. The recoveries were in the range of 76.2–110% with relative standard deviations (n = 5) of 2.3–9.7%. On the basis of these results, magnetic perhydroxy-cucurbit[8]uril materials with selective enrichment capability have good potential on the analysis of ultratrace targets from complicated sample matrixes.
研究领域:
植物学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Cuihua Hu,Ningning Ma,Fei Li,Yu Fang,Yao Liu,Linlu Zhao,Shanpeng Qiao,Xiumei Li,Xiaojia Jiang,Tiezhu Li,Fangzhong Shen,Yibing Huang,Quan Luo,Junqiu Liu
Highly stable giant supramolecular vesicles were constructed by hierarchical self-assembly of cucurbit[8]uril (CB[8])-based supra-amphiphiles for photoresponsive and targeted intracellular drug delivery. These smart vesicles can encapsulate the model drugs with high loading efficiencies and then release them by manipulating photoswitchable CB[8] heteroternary complexation to regulate the formation and dissociation of supra-amphiphiles that cause dramatic morphological changes of the assemblies to achieve remote optically controlled drug delivery. More importantly, the confocal microscopy analysis, cellular uptake experiment, and cell viability assay have shown that the giant vesicles are able to maintain the structural integrity and stability within actual cellular environments and exhibit obvious advantages for intracellular drug delivery such as low toxicity, easy surface modification for tumor-targeting selectivity, and rapid internalization into different human cancer cell lines. A synergistic mechanism that integrates multiple pathways including energy-dependent endocytosis, macropinocytosis, cholesterol-dependent endocytosis, and microtubule-related endocytosis was determined to facilitate the internalization process. Moreover, cytotoxicity experiments and flow cytometric analysis have demonstrated that the doxorubicin hydrochloride-loaded vesicles exhibited a significant therapeutic effect for tumor cells upon UV light irradiation, which makes the photoresponsive system more promising for potential applications in pharmaceutically relevant fields.
研究领域:
药学
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纳米技术
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生物医学工程
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材料科学
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药物递送系统
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光化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 47
·22133-22140
·
IF 3.591
Huaming Feng,Yang Luo,Ming Liu,Qing Chen,Zhu Tao,Xin Xiao
Cucurbit[n]urils (Q[n]s) are essential members of the supramolecular organic framework family owing to their distinct structure. In this study, Q[8] was employed as a building block and a Q[8]-based porous supramolecular assembly (Q[8]–PSA) was prepared by simple crystallization in an aqueous HCl solution. This Q[8]–PSA was constructed through the outer surface interaction of Q[8] and hydrogen bonding between the lattice water molecules and portal carbonyl oxygens of Q[8]. Q[8]–PSA provides a uniform pore structure, large specific surface area, and uniform pore size, which was characterized by single crystal XRD, TEM, SEM, and FT-IR spectroscopy. Following this, the obtained Q[8]–PSA was employed to evaluate its adsorption property towards 8 drugs with varying shape and size. Moreover, the controlled release of drugs from Q[8]–PSA was also investigated at pH 4.5 and 6.8 using an orbital oscillator.
研究领域:
材料科学
·
药物递送
·
超分子化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）