PEST MANAGEMENT SCIENCE
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2024
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IF 3.8
Shan-Cheng Yi,Xin-Hui Chen,Yu-Hang Wu,Juan Wu,Jia-Qing Wang,Man-Qun Wang
BACKGROUND The important wood-boring pest Batocera horsfieldi has evolved a sensitive olfactory system to locate host plants. Odorant-binding proteins (OBPs) are thought to play key roles in olfactory recognition. Therefore, exploring the physiological function of OBPs could facilitate a better understanding of insect chemical communications. RESULTS In this research, 36 BhorOBPs genes were identified via transcriptome sequencing of adults' antennae from B. horsfieldi , and most BhorOBPs were predominantly expressed in chemosensory body parts. Through fluorescence competitive binding and fluorescence quenching assays, the antenna-specific BhorOBP28 was investigated and displayed strong binding affinities forming stable complexes with five volatiles, including (+)-α-Pinene, (+)-Limonene, β-Pinene, (−)-Limonene, and (+)-Longifolene, which could also elicit conformation changes when they were interacting with BhorOBP28. Batocera horsfieldi females exhibited a preference for (−)-Limonene, and a repellent response to (+)-Longifolene. Feeding dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of BhorOBP28 , and could further impair B. horsfieldi attraction to (−)-Limonene and repellent activity of (+)-Longifolene. The analysis of site-directed mutagenesis revealed that Leu7, Leu72, and Phe121 play a vital role in selectively binding properties of BhorOBP28. CONCLUSION By modeling the molecular mechanism of olfactory recognition, these results demonstrate that BhorOBP28 is involved in the chemoreception of B. horsfieldi . The bacterial-expressed dsRNA delivery system gains new insights into potential population management strategies. Through the olfactory process concluded that discovering novel behavioral regulation and environmentally friendly control options for B. horsfieldi in the future. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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化学生态学
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昆虫生理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Xinxin Zhang,Xiao Wang,Shiwen Zhao,Kui Fang,Zhun Wang,Jianan Liu,Jinghui Xi,Shang Wang,Juhong Zhang
The rice water weevil (RWW), Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive rice pest that threatens the rice industry worldwide. Odorant receptors (ORs) and odorant receptor coreceptors (Orcos) play an important role in the process of insects’ whole life activities; however, there are no related functional studies on RWW. On this basis, a heterologous study of LoryOR20/LoryOrco in Xenopus laevis oocytes was performed to detect the effects of certain natural compounds on RWWs and four active compounds were found. Electroantennogram (EAG) recordings and a behavior test showed that RWWs exhibited a significant response to phenylacetaldehyde (PAA) and an EAG measurement of dsRNA-LoryOR20-treated RWWs revealed a significant decrease in response to PAA. Our results revealed an olfactory molecular mechanism for the recognition of PAA by RWWs, thus providing a potential genetic target at the peripheral olfactory sensing level, contributing to the development of novel control strategies for pest management.
研究领域:
昆虫学
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分子生物学
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农业科学
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DOI
PubMed
高影响因子
PLANT BIOTECHNOLOGY JOURNAL
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2025
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IF 10.5
Xuefeng Wei,Guangjin Fan,Song Yang,Xianchao Sun,Lin Cai
Summary Delivery of exogenous nucleic acid to intact plants is a desirable but challenging technology due to the dominant transport barrier posed by the plant cell wall. Here, we found that three different morphologies of g-C 3 N 4 nanomaterials synthesized from urea can assist in the delivery of exogenous nucleic acids into mature leaf cells of Nicotiana benthamiana . Among these, g-C 3 N 4 carbon dots (CDs) showed a higher ability to deliver exogenous nucleic acid compared to nanoporous and g-C 3 N 4 nanosheets. The delivery ability of exogenous dsRNA and plasmid DNA by g-C 3 N 4 could also be restrained by stomatal closure and the endocytosis pathways in plant cells. Furthermore, the coupling of g-C 3 N 4 CDs with dsCP (CDs@dsCP), which is the dsRNA matching a specific fragment of the Coat protein -encoding gene of TMV, resulted in a superior antiviral effect compared with other morphologies of g-C 3 N 4 @dsRNAs and other loaded dsRNAs, which match Replicase , RNA-dependent Replicase or Movement protein of TMV. Significantly, a single spray of CDs@dsCP provided virus protection for at least 5 days. In addition, the g-C 3 N 4 CDs and g-C 3 N 4 CDs@dsRNA had no adverse effects on plant growth and development. Overall, our study presents a novel biocompatible and convenient tool for gene expression or gene silencing in intact plant leaves by spraying g-C 3 N 4 nanomaterials encapsulated with DNA or dsRNA, the efficiency of which is affected by the morphology of the g-C 3 N 4 nanomaterial, stomatal state and plant endocytosis pathway, and a highly promising solution for plant virus disease, which is an unsolved problem in plant disease control.
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DOI
PubMed
高影响因子
Arabian Journal of Chemistry
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2022
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IF 6.212
Jie Zhao,Yan Gong
RNA plays a crucial role in biochemical processes. RNAs with abasic sites (AP-RNA), as one of RNA lesions, will cause serious negative consequences. Efficient and sensitive detection of AP-RNA deserves but does not receive enough attention. Water-soluble uridine triphosphate (UTP)-capped Mn-doped ZnS quantum dots (QDs), which have excellent room-temperature phosphorescence (RTP) properties, were prepared by wet-chemical method. The UTP on the surface of QDs could form a precipitation complex with amiloride (AMI) to quench the RTP. Meanwhile, abasic double-stranded RNA (AP-dsRNA) with a high affinity to AMI could dissociate AMI from the surface of UTP-QDs, resulting in RTP recovery. In this process, a highly sensitive and selective RTP-detection system for AP-dsRNA was constructed with AMI as the modulation factor and UTP-QDs as the luminescence matrix. RTP was turned off and on for the determination of AP-dsRNA, and the interference of background fluorescence and scattered light was effectively avoided during complex formation and aggregation. Steric hindrance of the conformation of AP-dsRNA provided efficient selectivity. The detection limit for AP-dsRNA was 0.86 nM, the relative standard deviation was 2.1%, and the recovery of biological samples with AP-dsRNA addition ranged from 95% to 103% at optimal conditions. The system is expected to provide new methods for environmental monitoring, early disease screening, and evaluation of gene editing efficiency.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
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