Journal of Ocean University of China
·
2015
·Vol. 14
·期 4
·695-702
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IF 0.558
Xu Houguo,Huang Lina,Liang Mengqing,Zheng Keke,Wang Xinxing
A 3-month feeding experiment was conducted in an in-door seawater system to investigate the effect of dietary vitamin E (Ve) on the sperm quality of turbot ( Scophthalmus maximus ). D-α-tocopherol acetate was supplemented to the basal (control) diet (65.14 mg kg −1 Ve) to obtain low and high levels of dietary Ve (244.60 mg kg −1 , LVe; 721.60 mg kg −1 , HVe). Compared with the control, sperm concentration was significantly increased in Ve-supplemented groups (LVe and HVe); while relative sperm volume and testis-somatic index were significantly increased in group HVe only. Sperm motility duration was significantly longer in group HVe than in the control, but no significant difference was observed in percent motility among groups. Sperm size, the uniformity of mitochondrial size, and the integrity of mitochondria cristae and plasma membrane were improved by dietary Ve, especially in HVe. The content of Ve in testis and liver as well as polyunsaturated fatty acids in sperm increased with dietary Ve. These results suggested that dietary Ve, especially at the high level (721.60 mg kg −1 ), significantly improved sperm concentration and motility duration and maintained normal sperm morphology of turbot.
研究领域:
水产养殖学
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营养学
·
生殖生物学
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DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2022
·
IF 3.682
Xifa Lan,Quanling Guo,Zhiwei Liu,Kai Liu,Jinfeng He,Ruyu Li,Haotian Sun,Wenxiu Yao,Longgang Wang
Chemotherapy is a major cancer treatment that uses antitumor drugs to kill fast-growing cancer cells. Many kinds of drug carriers have been developed to deliver and achieve controlled release of small-molecule therapeutic agents. However, many therapeutic agent carriers need complex preparation process. The natural polypeptides may serve as proper drug carriers. More specifically, polymyxin E (PE) is a kind of natural antibiotic lipopeptides. It is commonly used to treat infections caused by multidrug-resistant Gram-negative bacteria. Herein, we present a facile method to prepare DOX-loaded polymyxin E micelles (PE-DOX micelles) to enhance the therapeutic effect of anticancer drug doxorubicin (DOX). The hydrodynamic sizes and zeta potential of the prepared nanomedicine (PE-DOX micelles) were 142.0 nm and 6.47 mV, respectively. The release of DOX from PE-DOX micelles was faster at pH 5.5 than that at pH 7.4. Furthermore, PE exhibited negligible cytotoxicity to A549 cells and HeLa cells within 50 μg/mL, while PE-DOX micelles caused higher cytotoxicity than that of free DOX. Moreover, the intravenously injected PE-DOX micelles showed good biocompatibility and obvious antitumor effect after 14 days’ treatment in vivo. The PE-DOX micelles have great potential to be used as anticancer agent in the future.
研究领域:
药学
·
肿瘤学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Chengcheng Tang,Yiran Song,Zhuang Wang,Da Wang,Xinyu Yao,Yuan Meng,Yang Li,Li Yang
Growing multidrug bacterial resistance has become a life-threatening problem. Polymyxins are a “last resort” antibiotic against Gram-negative bacteria (Gram-ne − ), but exhibit weak activity against Gram-positive bacteria (Gram-po + ) caused by different bacterial membrane structures. Herein, it is proposed that the challenge can be overcome by hydrophobically modified polymyxin E for enhancing the hydrophobic interaction with the membrane. After synthesizing mono-and di-fatty acyl conjugates to Thr residues of polymyxin E, seven new polymyxin analogs were screened out with in vitro activity against Gram-ne − and Gram-po + . Among them, di-caprylic acid-modified (comp. 2 ) and mono-linoleic acid-modified (comp. 3) compounds with the most potent activity against both bacteria. Moreover, the membrane research showed that polymyxin analogs obtain activity against Gram-po + by higher ability to dispute cell membrane and wall than polymyxin E. Additionally, the results showed a trend of increasing hydrophobicity of analogs showing the tendency to rise up at the beginning and declining in later against Gram-po + and gradually decreasing activity against Gram-ne − , hence, we speculated that controlling Log P at 1–3 helps to obtain broad-spectrum antibiotic. Crucially, the maximum tolerated doses (MTD) of analogs were significantly higher than polymyxin E, and reduced the nephrotoxicity. As expected, hydrophobic modification polymyxin E not only enhance the activity of Gram-po + , but also could retain the activity of Gram-ne − . This study offers more insights into the synthesis of new antibiotics based on polymyxin E structure, especially broad-spectrum antibiotics.
研究领域:
微生物学
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药理学
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生物化学
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DOI
高影响因子
THERMOCHIMICA ACTA
·
2023
·
IF 3.5
Ningrui Zhao,Jumei Xu,Zuoxiang Zeng,Weilan Xue
The density of (E)-isobutyl cinnamate at 297.95–535.20 K was measured using a capillary pycnometer and linearly related to temperature. The vapor pressure of (E)-isobutyl cinnamate at 420.15–570.05 K was measured by the ebulliometric method, and correlated by Antoine equation with an average relative deviation of 1.11 %. The vaporization enthalpy Δ vap H (T b ) of (E)-isobutyl cinnamate at normal boiling point was obtained based on the Clausius-Clapeyron equation. The standard vaporization enthalpy Δ vap H (298.15 K) was calculated to be 73.67±0.5 kJ·mol −1 by Othmer's method and verified by the Corresponding State Group Contribution (CSGC) method. The heat capacities at saturation vapor pressure ( C SL ) of (E)-isobutyl cinnamate were measured at 298.15–533.15 K by an automatic calorimeter and correlated by a quadratic polynomial.
研究领域:
物理化学
·
热力学
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产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Kexin Cui,Zhang Fangming,Tianzi Shi,Siyu Zhao,Yixuan Zhou,Xiong Liu,Yong Hu,Zhaoyu Hu,Li Kong,Zhiping Zhang
Ionizable lipids are crucial for the effective delivery of mRNA by using lipid nanoparticles (LNPs). Endowing ionizable lipids with tailored biological properties could potentially augment the therapeutic efficacy of mRNA-based treatments. Herein, a functional vitamin E (VE)-based lipid library with distinct head groups was designed and synthesized. Due to the presence of VE, these lipids inherently exhibited immunomodulatory properties, including the promotion of cellular uptake, dendritic cells maturation, and antigen presentation. Through iterative optimization of the LNP components and the architecture of ionizable lipids, the correlation between the structure of ionizable lipids and their mRNA delivery efficiency has been established, leading to the finding of the most effective delivery formulation. Benefiting from the high mRNA delivery efficiency and the immunomodulatory function of LNPs themselves, VE-based LNPs have demonstrated complete remission in colon cancer by delivering mIL-12, which offered a beneficial combination with immune checkpoint blockade. The proposed functional LNPs were anticipated to furnish potential delivery systems for mRNA-based cancer treatments.
研究领域:
生物技术
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肿瘤学
·
药剂学
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产品关联
DOI
PubMed
高影响因子
International Journal of Peptide Research and Therapeutics
·
2017
·Vol. 25
·期 1
·161-168
·
IF 0.904
Yu Zhiliang,Zhang Ling,Qin Wangrong,Yin Jianhua,Qiu Juanping
Polymyxin E (colistin) has been considered as an important peptide antibiotic for clinical treatment of Gram-negative pathogens by selectively targeting lipopolysaccharide on the outer membrane. Recent reports showed that colistin can also kill Gram-positive bacteria, but the mechanism of colistin against Gram-positive bacteria remains unclear. In this study, we found that colistin can kill its producer Paenibacillus polymyxa , a Gram-positive bacterium, by both disrupting its cell integrity and inducing oxidative stress. Enhancement of the cell permeability is the major action of colistin against P. polymyxa . In relative, colistin-induced oxidative stress plays an associated role in killing of P. polymyxa . Notably, we, for the first time, found that exogenous catalase is able to intensify the colistin-induced killing by inhibiting the intracellular catalase activity and thus increasing oxidative stress. Our findings would shed light on a not yet clearly described bactericidal mechanism of colistin against Gram-positive bacteria. More importantly, our findings pave the road for the future to lower clinical use of colistin for avoiding bacterial resistance.
研究领域:
微生物学
·
药理学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2020
·
IF 4.845
Longbing Ling,Muhammad Ismail,Zhihao Shang,Yihui Hu,Benhong Li
Irinotecan (Ir) is a potent antitumor chemotherapeutics in clinic and used for the treatment of a various cancers, but the degree of its application is critically limited by toxic side-effects and marked heterogeneities. Nano-formulation of prodrugs, based on “all-in-one” carrier-free self-assemblies offers an effective approach to alter pharmacokinetics and safety profiles of cytotoxic agents. In this study, a novel vitamin E succinate-based formulation of Ir (VES-Ir) combined with nanoscaled characteristics and synergistic combination was constructed through esterification. The conjugation makes amphiphilic VES-Ir prodrug self-assemble into nanoparticles with a fine diameter (VES-Ir NPs, 75.4 nm) of spherical morphology. Furthermore, VES-Ir NPs with a 1:1 drug-to-drug ratio was demonstrated to possess respectable physiological stability within 72 h test, while can react to pH/esterase-sensitive drug release in lysosomes internalized into tumor cells, potentially highlighting their alleviating side effects. Compared with single and mixture drugs administration, the nanoformulated VES-Ir NPs codelivered both VES and Ir with different anticancer mechanisms to induce the highest suppress proliferation of MCF-7 (IC 50 0.18 μM) and A549 (IC 50 0.29 μM) cells in a synergistic way (CI < 1). More importantly, the formulating nanoparticulate Ir is to significantly enhance its bioavailability in vivo with long retention time in bloodstream and thereby, resulting the superior tumor inhibitory rate (TIR) of 85.2% versus controls. This simple nanoformulation of Ir drug deprived from VES conjugation, together with self-delivery and synergistic property, may provide an effective strategy for multiple chemotherapeutics delivery to treat cancers or other diseases.
研究领域:
药剂学
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肿瘤学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 42
·15484-15488
·
IF 4
Xiangyu Zhang,Yunlei Chen,Yongfang Sun,Fei Wang,Xiao-Dong Wen,Tian-Nan Ye
Wide application of novel materials often requires low-cost preparation methods. In this study, we present a simplified and facile preparation method for the [Ca24Al28O64]4+(e−)4 electride material (C12A7:e−). Successful preparation of the C12A7:e− electride was confirmed by XRD patterns and magnetic behavior analysis. The concentration of electrons in the prepared C12A7:e− powder was calculated to be approximately 2.23 × 1021 cm−3, as evaluated by iodometry and TPD. DFT calculations provided insight into the unique electronic structure of C12A7:e−. Additionally, the substitution of the Ca reductant with CaH2 led to a reduction in the solid-state reaction temperature from 1100 to 950 °C, which can be attributed to thermodynamic effects such as a reduction in ΔG°.
研究领域:
材料科学
·
化学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2023
·
IF 3.9
Qianli Sheng,Min Yuan,Dan Wang,Yuanqi Kou,Lei Liu,Yan Chen,Shiyong Song
Vitamin E derivatives are particularly effective in chemotherapy drug development because they are nontoxic, biocompatible, and selective. Among them, α-tocopheryl succinate (α-TOS) can act synergistically with some chemotherapeutic agents. However, its hydrophobicity limits its systemic administration, and localized formulations are not available. Herein, we developed an injectable hydrogel based on self-assembled micelles of a triblock amphiphilic derivative of α-TOS (PEG-2VES), in which doxorubicin (DOX) was encapsulated in the core of the micelles for combined chemotherapy. A molecule of α-TOS was grafted onto each end of poly(ethylene glycols) (PEGs) of different lengths. Hydrogels were prepared by dissolving the polymers or the DOX-loaded micelles in water at room temperature. The subcutaneously injected hydrogels kept their shape and sustainably released the payloads over 7 days without any noticeable inflammatory response. In vitro and in vivo results confirmed the synergistic antitumor effects of the hydrogel and loaded drug. Furthermore, DOX-loaded hydrogels showed greater therapeutic efficiency and fewer toxic side effects than DOX alone. Overall, this hydrogel acts as a multifunctional system that can deliver drug, improve the therapeutic effect, and minimize drug toxicity.
研究领域:
药学
·
生物医学工程
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2025
·
IF 2.1
Mingzhe Li,Yuyang Zhou,Hua Zhou,Dingqiang Lu
Vitamin E is an important fat soluble antioxidant, its acetate is the main derivative product of vitamin E. Using metal organic framework material ZIF-8 as a catalyst, vitamin E acetate (VEA) was prepared by catalyzing the esterification reaction of D-α-tocopherol and acetic anhydride in a solvent-free system. Firstly, the preparation conditions of metal organic framework materials were studied: different imidazole ligands, different zinc ion ligands, molar concentration ratio of Zn2+ to 2-methylimidazole(2-mIM), and preparation time. The results showed that the catalytic performance of ZIF-8 was optimal when Zn(CH3COO)2·2H2O was used as the zinc source, 2-mIM was used as the imidazole ligand, the molar concentration ratio of Zn2+ / 2-mIM was 1:20, and the preparation time was 1 hour. The yield of vitamin E acetate was 86.85%. The synthesis process of VEA was optimized by single factor experiments such as substrate molar ratio, reaction time, dosage of nano enzyme, reaction temperature and so on. The results showed that the yield of vitamin E acetate reached 96.31% under the optimal conditions of n (D-α-tocopherol) =1mmol, [n (D-α-tocopherol) : n (Acetic anhydride)] =1:15, reaction time 24 h, ZIF-8 dosage 0.05 g, reaction temperature 50 °C.
研究领域:
化学
·
生物学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Yujuan Xu,Wu Lei,Juan Su,Jinjin Hu,Xiaodan Yu,Tong Zhou,Yun Yang,Daniel Mandler,Qingli Hao
A fast, ultrasensitive electrochemical sensing platform based on graphitic carbon nitride-electrochemically deposited-poly(3,4-ethylenedioxythiophene) (g-C 3 N 4 -E-PEDOT) composite was constructed by in-situ electropolymerization and applied for the quantitative determination of acetaminophen (AP). E-PEDOT was introduced as the conducting matrix for developing g-C 3 N 4 composite to complement the poor conductivity disadvantage of g-C 3 N 4 . The strong affinity and synergetic effect between g-C 3 N 4 and E-PEDOT, which were analyzed by PM6 computational calculation, highly improved the electron transfer property and remarkably enhanced the electrochemical catalytic activity of the composite. The g-C 3 N 4 -E-PEDOT modified glassy carbon electrode (GCE) demonstrated better electrocatalytic activity towards the oxidation of AP than bare, g-C 3 N 4 and E-PEDOT modified ones. Under the optimized conditions, the oxidation peak currents at the g-C 3 N 4 -E-PEDOT/GCE increased linearly in the concentration range of AP from 0.01 to 2 μM and 2–100 μM, and an ultra-low limit of detection (LOD) of 34.28 nM was obtained (S/N = 3). In addition, the g-C 3 N 4 -E-PEDOT/GCE was successfully applied for the AP determination in the clinical human serum, and also exhibited excellent selectivity, reproducibility and stability. Except the novel AP determination approach, moreover, this work provided a new electrochemical application angle of graphitic carbon nitride theoretically as well as experimentally.
研究领域:
分析化学
·
材料科学
·
医学检测
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产品关联
DOI
高影响因子
Particuology
·
2021
·
IF 3.067
Youhong Zhang,Yinpei Zhu,Leslie S. Loo,Jianguo Yin,Kean Wang
Molecularly imprinted polymer (MIP) beads imprinted with vitamin E (V E ) were synthesized via a two-step swelling polymerization method, using methacrylic acid (MAA) and other functional monomers together with polystyrene (PS) microspheres as the substrate. In initial trials, different functional monomers were assessed, along with a variety of solvents and imprinting ratios. The MIP MAA sample exhibited the highest performance and was characterized and also assessed with regard to V E adsorption under various experimental conditions. This material exhibited reasonably good sphericity, porosity and stability, along with an excellent adsorption capacity of 46 mg/g. This was much higher than the value for the corresponding non-imprinted polymer made with the same monomer, and 20% higher than that for an acrylamide-based MIP synthesized using a precipitation polymerization method. The core-shell structured MIP MAA also demonstrated fast adsorption kinetics, a low pressure drop and good reusability in fixed bed experiments. The V E adsorption equilibrium, selectivity, kinetics, thermodynamics and regeneration capacity of this material were also examined and are reported herein.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）