BIOSENSORS & BIOELECTRONICS
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2020
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IF 10.257
Hua Ma,Kaiwen Chen,Bo Song,Zhixin Tang,Yundi Huang,Ting Zhang,Huanan Wang,Wenping Sun,Jingli Yuan
Development of fluorescent/luminescent probes for rapid, selective and sensitive detection of reactive oxygen species (ROS) is of great significance for understanding the roles of ROS in pathophysiological processes. In the present research, a visible-light-excitable Eu 3+ complex-based probe, Eu(L) 3 (DPBT), is designed and synthesized for the time-gated luminescence (TGL) determination of hypochlorous acid (HClO) in vitro and in vivo . The proposed probe exhibits a rapid, selective and sensitive TGL response to HClO, and excellent localization of mitochondria in living cells with low cytotoxicity. These features allow the probe to be used for the TGL sensing and imaging of HClO formation in mimic inflammatory cells at a subcellular level, as well as in endotoxin-induced liver injury and rheumatoid arthritis in live mice. In addition, by immobilizing the probe in the PEG hydrogel, the smart sensor films with rapid response to HClO were prepared, and successfully used for the real-time monitoring of HClO generation in mouse wounds, in order to distinguish the infected wounds from acute ones. Overall, this study provides a useful tool for the clinical monitoring and treatment of wound diseases.
研究领域:
生物化学
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医学影像学
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分子生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Zheng Cheng,Xinfang Liu,Beibei Zhao,Xu Liu,Xiaorui Yang,Xiaoyu Zhang,Xun Feng
The on-site detection of mancozeb in food samples holds immense value for food safety. A red-fluorescent europium complex ( Eu-PYDC-Phen ) has been prepared and employed as a fluorescence probe for mancozeb detection. The optimized probe suspension exhibits excellent detection performances, including a wide linear range (0–0.24 mM), low detection limit (65 nM), rapid response (2 mins) and high selectivity . Moreover, a portable detection platform was carefully designed, integrating the Eu-PYDC-Phen -based fluorescent test strips with smartphone color recognition software. This innovative platform enables visual and on-site detection of mancozeb in tomato, apple, and lettuce, achieving satisfactory recovery rates (90.34 to 106.50%). Furthermore, the integration of machine learning techniques based on hierarchical clustering algorithm has the potential to further improve the prediction and decision-making efficiency in mancozeb detection. This work provides an economical, convenient, and reliable strategy for on-site detection of pesticide in agricultural products, thereby making a meaningful contribution to food safety.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2015
·Vol. 7
·期 5
·1147-1157
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IF 5.52
Chaolong Yang,Shaojun Liu,Jing Xu,Youbing Li,Mingyong Shang,Lei Lei,Guoxia Wang,Jian He,Xuanlun Wang,Mangeng Lu
A novel efficient antenna Eu-complex Eu(TTA)2Tpy-OCH3·2H2O, based on the terpyridyl derivative 4′-(4-methoxyphenyl)-2,2′:6′,2′′-terpyridine(Tpy-OCH3) as the ancillary ligand, has been synthesized, structurally characterized, and its photophysical properties have been examined. Upon irradiation at the ligand-centered band in the range of 200–450 nm, the new Eu-complex displays bright red luminescence, irrespective of the medium. A Sparkle/RM1 calculation was utilized for predicting the ground-state geometries of this complex. Theoretical Judd–Ofelt and photoluminescence parameters, including quantum efficiency, as predicted from this model are in good agreement with the experimental values, proving the efficiency of this theoretical approach as implemented in the LUMPAC software (http://lumpac.pro.br). The kinetic scheme for modelling energy transfer processes shows that the main donor state is the ligand triplet state and that energy transfer occurs on both the 5D1 (20.7%) and 5D0 (79.3%) levels. As an integral part of this work, the synthesis, characterization, and luminescence properties of poly(vinyl butyral) (PVB) polymer films doped with the Eu-complex are also reported. After the Eu-complex Eu(TTA)2Tpy-OCH3·2H2O was doped into the PVB matrix to form the films, the PVB polymer matrix, acting as a co-sensitizer for Eu3+ ions, enhances the luminescence lifetimes and quantum efficiencies of the complex in comparison with its precursor complex. The new luminescent Eu/PVB films therefore show considerable promise for polymer light-emitting diode and active polymer optical fiber applications. To the best of our knowledge, this is the first report which studies in detail the photophysical properties of doped europium fluorescent films based on PVB as the polymer matrix.
研究领域:
材料科学
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光学
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化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）