APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2022
·Vol. 194
·期 7
·2919-2930
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IF 3.094
Gu Yuanjie,Guo Jianming,Zhou Xin,Xu Yong
Xylo-oligosaccharides have great value in food, feed fields. Previous studies have shown that organic acids catalyze the hydrolysis of xylan-rich sources for the production of xylo-oligosaccharides. In this study, gluconic acid of aldonic acid generated xylo-oligosaccharides via hydrolysis of xylan from corncob. In order to maximize efficiency of xylo-oligosaccharides production, the optimum conditions was ascertained by Box-Behnken design-based response surface methodology. The developed process resulted in a maximum xylo-oligosaccharides yield of 57.73% using 4.6% gluconic acid at 167 °C for 28 min, which was similar to the predicted value and fitted models of xylo-oligosaccharides production. The results showed that the reaction temperature was crucial to xylo-oligosaccharides production, and by-product yields (xylose and furfural) could be effectively controlled by both reaction temperature and time. In addition, 44.87 g/L XOS was achieved by decreasing the solid-liquid ratio. Overall, the described process may be a preferred option for future high concentration xylo-oligosaccharides production.
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MOLECULAR ECOLOGY
·
2023
·
IF 4.9
Xiao-Li Bing,Zi-Jian Liang,Jia Tian,Xue Gong,Shao-Qiu Huang,Jie Chen,Xiao-Yue Hong
Insects are rich in various microorganisms, which play diverse roles in affecting host biology. Although most Drosophila species prefer rotten fruits, the agricultural pest Drosophila suzukii attacks ripening fruits before they are harvested. We have reported that the microbiota has positive and negative impacts on the agricultural pest D. suzukii on nutrient-poor and -rich diets, respectively. On nutrient-poor diets, microbes provide protein to facilitate larval development. But how they impede D. suzukii development on nutrient-rich diets is unknown. Here we report that Acetobacter pomorum (Apo), a commensal bacterium in many Drosophila species and rotting fruit, has several detrimental effects in D. suzukii . Feeding D. suzukii larvae nutrient-rich diets containing live Apo significantly delayed larval development and reduced the body weight of emerged adults. Apo induced larval immune responses and downregulated genes of digestion and juvenile hormone metabolism. Knockdown of these genes in germ-free larvae reproduced Apo-like weakened phenotypes. Apo was confirmed to secrete substantial amounts of gluconic acid. Adding gluconic acid to the D. suzukii larval diet hindered larval growth and decreased adult body weight. Moreover, the dose of gluconic acid that adversely affected D. suzukii did not negatively affect Drosophila melanogaster , suggesting that D. suzukii is less tolerant to acid than D. melanogaster . Taken together, these findings indicate that D. suzukii is negatively affected by gluconic acid, which may explain why it prefers ripening fruit over Apo-rich rotting fruit. These results show an insect's tolerance to microbes can influence its ecological niche.
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JOURNAL OF CATALYSIS
·
2023
·
IF 7.3
Tianliang Xia,Meiting Ju,Hengli Qian,Xinyu Bai,Ruite Lai,Chao Xie,Guanjie Yu,Yao Tang,Chengxu Wang,Qidong Hou
Peroxymonosulfate (PMS) activation is a prospective technique for pollutant degradation, but less attention has been paid to the tunable modulation and effective utilization of reactive oxygen species in selective oxidation. Herein, we develop a photocatalytic Fenton-like system with Fe single-atom supported on carbon nitride that generates singlet oxygen ( 1 O 2 ) as dominant active species to selectively oxidize glucose to gluconic acid. The loading of atomic Fe onto carbon nitride can notably aggrandize the light absorption and boost the photon-generated carriers transfer efficiency. With PMS and simulated sunlight, the Fe single-atom catalyst afforded a glucose conversion of 36.3% with gluconic acid selectivity up to 91.6%, as is superior to the state-of-the-art catalytic systems. Control experiments and theoretical analysis reveal that the excellent catalytic performance of Fe single-atom supported on carbon nitride is resulted from the enhanced photocatalytic activity with better PMS activation ability.
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BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Hongsen Zhang,Jian Zhang,Jie Bao
This study reported a high titer gluconic acid fermentation using dry dilute acid pretreated corn stover (DDAP) hydrolysate without detoxification. The selected fermenting strain Aspergillus niger SIIM M276 was capable of inhibitor degradation thus no detoxification on pretreated corn stover was required. Parameters of gluconic acid fermentation in corn stover hydrolysate were optimized in flasks and in fermentors to achieve 76.67 g/L gluconic acid with overall yield of 94.91%. The sodium gluconate obtained from corn stover was used as additive for extending setting time of cement mortar and similar function was obtained with starch based sodium gluconate. This study provided the first high titer gluconic acid production from lignocellulosic feedstock with potential of industrial applications.
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Inorganic Chemistry Frontiers
·
2025
·
IF 6.4
Ting-Ting Shen,Jing Liu,Fang-Yuan Bai,Wei-Wei Xu,Xue Yong,Zhi-Rong Li,Jing-Ru Han,Jun Chen,Heng Zhao,Zhi-Yi Hu,Yu Li,Bao-Lian Su
Biomass photoreforming to coproduce sustainable hydrogen and valuable chemicals is a potential strategy for alleviating energy and environmental issues. However, the lack of bifunctional catalysts to efficiently achieve the “one stone kills two birds” scenario greatly limits its practical application. Herein, we rationally design a three-dimensionally ordered macro-porous structure (3DOM) CaTiO3 (CTO) to address the mass diffusion and light harvesting and to load ZnxCd1-xS (ZxC1-xS) quantum dots (QDs) realizing the selective glucose photoreforming process. The regulatable band gap of ZxC1-xS endows 3DOM CTO-ZxC1-xS composites with sufficient light absorbance and adjustable redox potentials. As a result, the optimized 3DOM CTO-Z0.5C0.5S delivers the best performance for sustainable hydrogen evolution from glucose photoreforming with the rate of 4.05 mmol g-1 h-1 and the apparent quantum efficiency (AQY) of 6.48% under monochromatic light of 365 nm. In particular, the well-developed photocatalysts simultaneously produce gluconic acid with the selectivity up to 83.8% from the targeted oxidation of terminal aldehyde group of glucose. The DFT calculations on Gibbs free energy change of HER and the energy difference between reactants and products of OER further reveal that the constructed Z-scheme heterojunction contributes to the spatial separation of photogenerated electrons and holes for a good quantum efficiency and liquid product selectivity. This work demonstrates a sustainable technology for the coproduction of hydrogen and value-added chemicals from photocatalytic biomass valorization.
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BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Junyu Wang,Qianqian Chen,Yuan Wang,Long Su,Zuotao Tan,Dong Liu,Shaohong You,Hongli Wu,Fei Cao
Multi-enzyme cascade valorizes starch-rich TCHRs into 5KGA; • NADP + regeneration built via Ga5DH-SBFA-CAT coupling; • Reverse activity suppressed, enabling efficient GA-to-5KGA conversion; • Fed-batch production of 5KGA reached 203 g/L with TTN of 9.5 × 10 4 . Herbal residues (HRs) are emerging as an important and sustainable biomass resource, driven by the diversification of global health concepts and the rapid growth in related consumption. Herein, we established a valorization route from starch-rich traditional Chinese herb residues (TCHRs) to high-value 5‑keto‑D‑gluconic acid (5KGA) via a multi-enzymatic cascade. Starch-rich TCHRs were first converted to gluconic acid (GA) through enzymatic hydrolysis and oxidation (>80% conversion). Subsequently, an oxidized cofactor regeneration system was established to efficiently convert GA into 5KGA by coupling gluconate-5-dehydrogenase (Ga5DH) with a synthetic bridged-flavin analogue (SBFA), thereby mitigating constraints arising from Ga5DH’s high reductive activity. This system catalyzed the irreversible dehydrogenation of GA to 5KGA, achieving complete conversion of 100 mM GA within 4 h (total turnover number, TTN = 1.0 × 10 4 ). Furthermore, upon scaling up in a 1-L bioreactor with continuous operation for 48 h, 203.3 g/L (950 mM) of 5KGA was produced with a TTN of 9.5 × 10 4 . This value-upgrading strategy provides an efficient and economically viable biotransformation pathway for starch-based non-edible biomass feedstocks. Download: Download high-res image (112KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jianming Guo,Yanran Liu,Xin Zhou,Dylan Liu,Kaixuan Huang,Yong Xu
A two-step process combining solvent extraction and hydrogen peroxide-gluconic acid (HP-GA) pretreatment was employed to effectively remove the barriers affecting the enzymatic saccharification of masson pine cellulose. Ethanol extraction removes bioactive phenolics and eliminates the negative effect of the following pretreatment and cellulose saccharification. The gluconic acid (GA)-based peroxide was formed by mixing hydrogen peroxide (HP) with GA, which effectively pretreated inert masson pine with the synergistic oxidative degradation and dissolution of lignin and hemicellulose. During the pretreatment, various organic peracid catalysts were detected such as peroxy-GA, peroxy-acetic acid, and peroxy-formic acid. After ethanol extraction at 80 °C and HP-GA pretreatment at 90 °C, 95.4 % of lignin and 69.2 % of hemicellulose could be removed from masson pine, and the enzymatic hydrolysis of cellulose resulted in 99.2 % glucose yield. Based on GA and the green-electric derived H 2 O 2 , this work provides an ecological and efficient pretreatment method to completely break down the critical biorefinery barriers on enzymatic saccharification of cellulose, especially concerning inert softwood-type lignocellulosic biomass.
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Catalysis Science & Technology
·
2022
·Vol. 13
·期 4
·991-999
·
IF 6.177
Jie Gao,Zhenfu Wang,Ranran Guo,Yang Hu,Xiaoyan Dong,Qinghong Shi,Yan Sun
A chemoenzymatic cascade system (GA&GOx@Au–SiO2) was fabricated by immobilizing Au nanoparticles onto mesoporous silica nanoparticles (SiO2) followed by the co-immobilization of glucoamylase (GA) and glucose oxidase (GOx) for the efficient production of gluconic acid from starch. The proximity effect of Au nanoparticles, GOx, and GA allowed rapid transport of the reaction intermediates (glucose and H2O2) and timely removal of H2O2 that was harmful to the enzymes, thus improving the yield of gluconic acid as well as stability of the biocatalysts. The yield of gluconic acid with GA&GOx@Au–SiO2 reached 97.8% within 30 min, much higher than the control systems for comparison. Besides, GA&GOx@Au–SiO2 maintained 83.9% of initial activity after 10 cycles of usage, also higher than that without immobilized Au nanoparticles (57.5%). Such a chemoenzymatic cascade system provides a new strategy for improving the performance of enzymatic reactions with H2O2 generation.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2022
·
IF 4.326
Huangying Wang,Junying Yan,Rong Fu,Haiyang Yan,Chenxiao Jiang,Yaoming Wang,Tongwen Xu
Bipolar membrane electrodialysis (BMED) is an environmentally friendly, high-effective technique for the cleaner production of gluconic acid. However, the unsatisfactory purity and low concentration of the regenerated base limit the applicable field of the byproduct for further utilization. In this study, BMED was applied for the cleaner production of gluconic acid from the perspective of the regenerated base. First, four types of cation-exchange membrane were screened for the BMED process by evaluating the performances of current efficiency, purity of regenerated base, and energy consumption. The BMED performances follow the order of CMX > TWEDC > CJMC-5 > CJMC-3. Furthermore, the effects of current density, the volume ratio between salt and base compartment on the BMED performances were optimized. A high base purity of 96.6% was obtained at a current density of 40 mA/cm2, while a high base concentration of 4.58 mol/L could be reached by applying a high volume ratio of 5:1 between the salt and base compartment. Moreover, the mechanism on the leakage of organic salts into the regenerated base was elucidated. The purity of the regenerated base was attributed to diffusion and electromigration. Diffusion dialysis experiments demonstrated that the permeability of gluconate through the CMX was 4.7 times of that through the BP-1. It was also found that the leakage of gluconate into the base could be alleviated at a low current density ranging from 20 to 50 mA/cm2. Finally, the regenerated base was subjected to the enzyme catalyst experiment for conversion of glucose into gluconate. This proof-of-concept study demonstrates that the regenerated base from the BMED process could be valorized to the upstream route for a closed-loop cleaner production.
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Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Songtao Cai,Jinman Wu,Yaokun Mo,Yuxuan Dai,Peng Liu,Jianxin Luo,Chunyan Zhang,Yifei Liu,Jianjun Liu,Qingwen Zhang,Fuming Du,Yi Wang,Hu Chen
A two-pot method is reported for the synthesis of ferric oxide-gluconic acid/β-cyclodextrin (Fe 3 O 4 -GA@β-CD). The proposed approach is a green one as the raw material is a natural product (sucrose) and no organic solvent is involved. Meanwhile, this novel composite was demonstrated for catalytic degradation of p-nitrophenol (PNP), a prototype toxic with high toxicity and low degradability. This is realized by H 2 O 2 activation induced by the heterogeneous Fenton-like catalysis of the as-prepared Fe 3 O 4 -GA@β-CD nano-microspheres. The results showed that the reaction rate constants of PNP degradation catalyzed by Fe 3 O 4 -GA@β-CD and Fe 3 O 4 -GA were 0.0879 and 0.0514 min −1 , respectively, suggesting improved catalytic performance of Fe 3 O 4 -GA@β-CD compared with Fe 3 O 4 -GA. This can be attributed to the cavity of β-CD, which can capture and include PNP molecules. The effects of pH value, H 2 O 2 concentration, catalyst load and hydroxyl radical quenching on the Fenton-like catalysis were explored and the optimized conditions (pH value = 2.0, H 2 O 2 concentration = 50 mM, and catalyst load = 1.0 g/L) were determined. Additionally, Fe 3 O 4 -GA@β-CD exhibited excellent reusability (catalytic degradation rate = 95.1% after five recycling times) as it can be recycled under external magnetic field owing to its good magnetic response (up to 18.41 emu/g).
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Journal of Energy Storage
·
2025
·
IF 9.8
Wei Zhang,Rui Mu,Zhenhe Chang,Chaoyue Zhang,Baohui Wang,Hongjun Wu,Di Gu
As clean and environmentally friendly renewable energy gradually replaces traditional fossil energy, which is highly polluting and non-renewable, flow batteries serve as scalable storage systems that balance energy input and output. Zinc–iron redox flow batteries (ZIRFBs) combine low cost with strong prospects for large-scale deployment. Acidic ZIRFBs benefit from higher Fe solubility and non-toxic electrolytes. However, environmental and operational challenges persist, including electrolyte cross-permeation, component corrosion, and the requirement for strong acids (leading to low pH) to inhibit Fe 3+ hydrolysis. Theoretical calculations indicate that environmentally friendly gluconic acid (GA), with its strong affinity for Fe 3+ , can prevent hydrolysis and transmembrane crossover, serving as an alternative to strong acids. Unlike previous acidic ZIRFBs with pH < 1 or even pH < 0, introducing GA into the cathode electrolyte facilitated the battery running stably under milder pH = 3–4 conditions for 133 cycles, with a running time of more than 125 h. The life of this battery could be extended to 215 % of the original one. The addition of GA is cost-effective and environmentally friendly, significantly enhancing battery performance. Crucially, it eliminates the need for traditional acidic ZIRFBs to operate in highly corrosive media, thereby creating new opportunities for their development.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2021
·
IF 19.503
Xinyu Bai,Qidong Hou,Hengli Qian,Yifan Nie,Tianliang Xia,Ruite Lai,Guanjie Yu,Mian Laiq Ur Rehman,Haijiao Xie,Meiting Ju
Photocatalysis has exhibited huge potential in many reactions, but the design of metal-free photocatalytic material for selective upgrading of biomass is rarely achieved. Here, we report that the metal-free photocatalyst consisted of nitrogen-deficient carbon nitride (BNCN) and chlorin e6 (Ce6) can efficiently and selectively oxidize glucose into gluconic acid and glucaric acid. Introducing nitrogen defects could significantly enhance the optical absorption and modulate the band structure. In addition, the combination of Ce6 with BNCN further improve the optical absorption property and promote effective separation of photon-generated carriers. As expected, the resultant Ce6@BNCN catalyst gave a total selectivity of gluconic acid, glucaric acid and arabinose as high as 70.9% at glucose conversion up to 62.3%, as is superior to previously reported photocatalytic systems. The mechanism for the enhancement of catalytic performance and the reaction pathway were revealed by experimental studies combined with DFT calculations.
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