PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2018
·
IF 1.241
Mingcong Shao,Lili Xiu,Haijiang Zhang,Jianying Huang,Xingwen Gong
Chitosan/cellulose-based beads (CCBs) for the affinity purification of histidine-tagged proteins were prepared from chitosan/cellulose dissolved in ionic liquid as a solvent, and their structures were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and thermogravimetric analysis. The affinity purification was used to separate hexahistidine-tagged (his-tagged) enhanced green fluorescent protein (EGFP) from Escherichia coli. The results showed that Zn2+–CCB exhibited more specific adsorption capacity toward the target protein compared with Ni2+–CCB and Cu2+–CCB. The maximum adsorption of EGFP was 1.84 mg/g of Zn2+–CCB, with 90% purity under the optimized conditions (ionic strength (1.0 M NaCl), pH (7.2) and imidazole concentration (500 mM)). In addition, a regeneration method for the sorbent was further developed by washing with ethylenediaminetetraacetic acid disodium and then reimmobilizing with metal ions. This technique is an alternative method for the purification of his-tagged proteins, making the process more economical, fast, stable, and large batch.
研究领域:
生物化学
·
蛋白质工程
✓ 已验证
DOI
PubMed
高影响因子
MACROMOLECULES
·
2023
·
IF 5.5
Yuanyuan Liu,Xiyu Wang,Caihui Luo,Jie Cai,Yuting Li,Ying Cao,Yuanli Cai
Salt bridges are predominant in protein construction and stabilization, yet largely unexplored for polymer nanoparticle synthesis. We herein report the use of l-histidine salt bridges to drive monomer preassembly and two-dimensional electrostatic self-assembly in aqueous photo-RAFT polymerization. l-histidine salt bridges drive the monomer clustering nucleation, complex coacervation, and Coulombic stabilization, leading to the 2 nm ultrasmall clusters and coacervate droplets. Homopolymerization leads to a precision two-dimensional electrostatic self-assembly via a droplet-monolayer-multilayer transition, i.e., salt-bridged homo-polymerization-induced self-assembly (PISA). Block copolymerization does not disturb the “salt-bridged homo-PISA” mechanism. Enhanced Coulombic repulsion via seeded polymerization of charged monomers using as-achieved multilayer lamellae (seeds) yields supercharged 5 nm ultrathin monolayer lamellae with high colloidal stability upon dilution, salting, and long-term storage, urgently needed for bioapplications. This work opens up a new avenue to use amino acid salt bridges for PISA synthesis of biologically important, yet hitherto inaccessible, salt-resistant ultrathin polyelectrolyte complex nanomaterials.
研究领域:
聚合物化学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Yanan Xu,Zhenhua Li,Dan Xiu,Guotao Sun,Christopher D. Snow,Yao Wang,Yanxin Wang,Laurence A. Belfiore,Jianguo Tang
Histidine Polypeptide-Hybridized Nanoscale Metal–Organic Framework (fH12/ZIF-8) can be produced effectively in water at room temperature without using any additives. The fluorescein label also provides a useful method to quantify the extent to which the anti-cancer drug doxorubicin (DOX) is loaded into or released from the resulting MOFs. In vitro studies have shown that fH12/ZIF-8 has the useful property of dissolving and controlled rates at low pH. It ruptured fastest in PBS buffer solution with pH 6.3, and the release rate reached 50% at 4 h. Furthermore, it showed that the addition of histidine polypeptides increases uptake by HepG-2 cells. This study integrates drug loading and detecting aspects into a single theranostic platform by a simple and efficient strategy, opening a new route for the construction of advanced drug delivery systems.
研究领域:
纳米技术
·
药学科学
·
生物医学工程
·
癌症研究
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yu-Jie Shi,Dan Zhao,Chang-Ying Hu,Zhi-Wei Wang
Bisphenol A diglycidyl ether (BADGE) from the epoxy-coated canned food can react with proteins/amino acids during high temperature sterilization process. The product formed with histidine (His), BADGE·H 2 O·His, was considered to have potential toxicity. In this study, we successfully prepared and identified this adduct. In vitro static gastrointestinal digestion experiment revealed the adduct had good stability. Additionally, the cytotoxicity of the adduct to gastric epithelial cells (GES-1) and two breast cancer cell lines (SUM-159 and MCF-7) was lower than that of BADGE. After incubating these cells with 200 μmolLexp.-1 BADGE for 24 h, cells viability decreased to 4.0 %, 5.2 %, and 12.7 %, respectively, while the adduct maintained cell viability at 89.1 %, 99.1 %, and 98.3 %, respectively. Furthermore, the cytotoxic mechanism of the adduct may be independent of oxidative stress, unlike BADGE, which showed an increase in oxidative stress damage and apoptosis with increasing concentration.
研究领域:
食品化学
·
毒理学
·
生物化学
·
细胞生物学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2024
·
IF 6.7
Yueling Xu,Xiujuan Gao,Han Yu,Yanjun Ding,Qinglin Li,Chengju Sheng,Mingming Guo
Polymeric materials exhibiting room-temperature phosphorescent (RTP) have attracted wide attention for their easy synthesis, low toxicity, and applications in various fields such as data encryption, cell imaging, information security and other fields. Nevertheless, the conventional ways of preparing such materials are mainly focused on doping, which may suffer from phase separation, poor compatibility, and lack of effective methods to promote intersystem crossing and suppress the nonradiative deactivation rates. Herein, a series of polymers with fluorescence and phosphorescence dual emission were prepared by simple radical solution copolymerization. Through rigid ion-bonded cross-linked networks and cross-linked hydrogen-bonded networks between the polymer chains, the non-radiative jumps and achieve ultra-long phosphorescence lifetimes were able to suppress. By suppressing non-radiative transitions through rigid ion-bonded cross-linked networks and intermolecular hydrogen-bonding networks between polymer chains, ultra-long phosphorescence is achieved. Impressively, a LRTP lifetime of up to 815.38 ms in polymers under ambientconditions is set up. Moreover, in this study, phosphorescence emission can be easily tuned by balancing ion radius and charge states. These results outline the construction of polymeric materials, endowing traditional polymers with new characteristics and promising potential applications.
研究领域:
材料科学
·
聚合物化学
·
光电材料
·
发光材料
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2014
·
IF 3.05
Cunliu Zhou,Huiqiong Ye,Tadayuki Nishiumi,Hao Qin,Conggui Chen
The objective of this investigation was to evaluate the effects of l -histidine on the stability of porcine hemoglobin concentrates during storage. The results indicated that the addition of l -histidine increased the oxyhemoglobin content and a* values but decreased the methemoglobin content and free iron content (P < 0.05) during storage. The addition of iron cation decreased both the oxyhemoglobin content and a* values but increased both the methemoglobin content and free iron concentration (P < 0.05). CD spectra revealed that the free iron content induced the transformation of the hemoglobin secondary structure from an α-helix (close structure) to a β-pleated, β-turn, and random coil arrangement (loose structure), while l -histidine weakened this behavior of the free iron. Infrared spectra demonstrated that the l -histidine coordinated with the free iron (Fe 2 + or Fe 3 + ) to give the corresponding complex at 25 °C and pH 7.3. Therefore, l -histidine enhanced the stability of hemoglobin concentrates by coordinating with free iron.
研究领域:
生物化学
·
医学工程
✓ 已验证
DOI
高影响因子
POLYMER
·
2026
·
IF 4.5
Yang Luo,Mingtao Zhu,Zhongying Zhao,Zixuan Wang
It is a great challenge to achieve hydrogels with robust mechanical strength, high elasticity, superior electrical conductivity and transparency for the application of flexible sensors. In this study, polyacrylicacid/histidine/AlCl 3 (PAA/His/Al 3+ ) hydrogel has been synthesized for the first time without any chemical crosslinking agents. The network structure was constructed via one-pot free radical polymerization by exploiting the carboxyl groups of acrylic acid and the carboxyl and amino functionalities of histidine that coordinate with Al 3+ ions. This hydrogel possesses high transparency, excellent mechanical properties (with a tensile elongation greater than 1400 %, a tensile strength up to 1.35 MPa, and a toughness exceeding 7.28 MJ m −3 ), good self-healing ability (with a self-healing tensile length greater than 76 % within 10 min), and a relatively high ionic conductivity (∼0.68 S m −1 ). The hydrogel exhibits stable and potent electrical signal responses to various mechanical stimuli, including puncture, impact and bending deformation. This material has great potential in the next-generation flexible electronics field and broad application prospects in the field of human-computer interaction.
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC ELECTRONICS
·
2018
·
IF 3.68
Lijun Zhang,Zhen Liu,Can Xiong,Lei Zheng,Yunsheng Ding,Hongbo Lu,Guobing Zhang,Longzhen Qiu
l -Histidine (L-His) is an essential bioactive amino acid , playing a significant role in several biological processes. Therefore, the accurate determination of L-His is of great significance to human health. In this work, a strategy was developed for the chiral detection of L-His using an organic electrochemical transistor (OECT) sensor based on molecularly imprinted polymer (MIP). MIP films were electrodeposited on OECT gate platforms. MIP films designed for specifically recognizing His combined with the amplified function of an OECT yielded highly sensitive and selective OECT biosensor . The properties of the resulting biosensor were characterized by various analytical methods. The results indicated that changes in channel current was proportional to D-His and L-His concentrations from 100 nM to 10 μM, with the detection limits of 10 nM and 100 nM (S/N > 3), respectively. Furthermore, the highly selective MIP film allowed to differentiate L-His from D-His or D-His from L-His. In terms of selectivity , the tests suggested apparent differences in channel current changes between L-His and D-His and interferences. The applicability of the biosensor for L-His detection in human urine demonstrated this sensor will be ideal transducers for disposable applications in the future.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Zhengbo Chen,Jianwei Nai,He Ma,Zhiqiang Li
Hourglass-like nickel hydroxide nanostructure was synthesized via a simple and facile route in solution. The as-prepared nickel hydroxide nanocrystals were characterized by X-ray diffraction (XRD), scanning electronmicroscope (SEM) and transmission electron microscopy (TEM) for the morphology study. The TEM experimental data indicate that the growth of hourglass-like nanocrystal is along the c axis of β-Ni(OH) 2 . The composites had a good electron transferring and biocompatibility. The l -histidine biosensor was fabricated by drop casting the Ni(OH) 2 /ethanol solution (2.5 g L −1 ) on the surface of the glassy carbon electrode (GCE). The prepared biosensor shows better catalytic performance compared with bulk material or other similar materials, with the detection limit of 80 nM and the linear range from 0.1 μM to 0.5 mM ( R 2 = 0.993) in a 0.1 M NaOH solution. The effects of electroactive interferents at the test conditions can be negligible which showed an excellent selectivity of the biosensor. The proposed method was also successfully applied for the determination of l -histidine in human blood serum sample. High sensitivity, excellent selectivity and ease of preparation make this sensor promising for the applications in the development of detecting some other amino acids and proteins.
研究领域:
生物化学
·
纳米材料
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2016
·
IF 2.75
Shufeng Shen,Ya-nan Yang,Yue Zhao,Yangyang Bian
Aqueous amino acid salts are attractive absorbents for carbon dioxide (CO 2 ) capture due to low volatility and resistance to oxidative degradation. The kinetics of CO 2 absorption into aqueous solutions of potassium histidinate (HisK) was investigated using a wetted wall column at concentrations ranging from 0.26 to 2.07 M and temperatures from 298 to 333 K. Density, viscosity of aqueous HisK and physical solubility of N 2 O in these solutions were measured. A temperature dependant ion parameter h His − was correlated to estimate Henry׳s constants of CO 2 . The zwitterion and termolecular mechanisms as well as ionic strength effect on reaction rate were applied to interpret the absorption rate of CO 2 in non-ideal systems. Under the pseudo-first-order regime, the overall reaction rate constants increase strongly with the increasing HisK concentration and temperature. HisK shows comparable chemical reactivity toward CO 2 with the industrial standard monoethanolamine (MEA). The partial reaction order with respect to HisK was found to be an average value of 1.26. Zwitterion deprotonation by water and HisK should be the rate-determining step. Two proposed models from zwitterion and termolecular mechanisms could successfully predict the experimental data within 12% AAD. The activation energy was calculated to be approximately 25.0 kJ mol −1 .
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 23
·11158-11165
·
IF 3.925
Jipeng Li,Yanzhi Sun,Yongmei Chen,Yang Tang,Junqing Pan,Pingyu Wan
In the present study, the preparation of a new MOF material His-MIL-53(Al) and its performance in the adsorption of Pb(II) from aqueous solution were investigated. The original material MIL-53(Al) was directly grafted with histidine to obtain the modified MOF material His-MIL-53(Al). The results show that His-MIL-53(Al) has excellent adsorption performance for Pb(II), and the adsorption process is in accordance with the pseudo first-order kinetics and the Langmuir adsorption model, with a maximum adsorption capacity of 50.13 mg g−1, which is much larger than the adsorption capacity of the original material MIL-53(Al). The experimental results also show that the prepared material has good recoverability. Our work demonstrates the applications of His-MIL-53(Al) as a promising material in the adsorption of heavy metals.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
LUMINESCENCE
·
2023
·Vol. 38
·期 6
·796-802
·
IF 2.9
Yuyu Guo,Yiqing Chu,Xiaojuan Sun,Songhao Cao,Xin Li,Xinyue Li
In view of the significance of nitrofurantoin, there is an urgent need for efficient analytical methods for accurate detection of nitrofurantoin. Considering their superior fluorescence performance and rarity of reports regarding nitrofurantoin detection by fluorescent silver nanoclusters (Ag NCs), Ag NCs with good stability and uniform size were synthesized through a simple method by protection of histidine (His) and reduction of ascorbic acid (AA). Based on the quenching by nitrofurantoin, Ag NCs were applied successfully in the detection of nitrofurantoin with high sensitivity. In the range of 0.5–150 μM, a linear relationship was found between ln( F 0 / F ) and nitrofurantoin amounts. Static quenching and inner filter effect were proved to be the main quenching mechanisms. Significantly superior selectivity and satisfactory recovery results in bovine serum indicate that Ag NCs provide a better choice for nitrofurantoin detection.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）