Journal of the Mechanical Behavior of Biomedical Materials
·
2023
·
IF 3.9
Bin Wang,Yanbin Zhao,Yangyang Li,Chengliang Tang,Peng He,Xiaowei Liu,Junyan Yao,Chenglin Chu,Bin Xu
There were defects like limited osteogenesis and fast drug release in traditional magnesium phosphate bone cement (MPC). In this study, we loaded icariin in a mesoporous nano silica container modified by polydopamine and then added it and citric acid into MPC (IHP-CA MPCs). The results indicate that IHP-CA MPCs have a long curing time, almost neutral pH value, excellent injectability , and compressive strength . In vitro experiments have shown that IHP-CA MPCs have good biocompatibility and bone promoting ability. These improvements provide feasible solutions and references for the clinical application of MPCs as implants .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 22
·5280
·
IF 4.2
Xiaoling Zhang,Yitong Wang,Tiantian Zhang,Ziqiao Yuan,Yongjun Wei
Epimedium Folium(EF) is a traditional Chinese herbal medicine, and its primary bioactive ingredients, such as icariin, are flavonoid glycosides. A rare EF flavonoid, baohuoside I, exhibits superior bioactivities and enhanced bioavailability compared to its metabolic precursor icariin. The biotransformation of icariin to baohuoside I can be effectively and specifically achieved by β-glucosidases. In this study, 33 candidate full-length β-glucosidase genes were screened from a previously built carbohydrate active enzyme (CAZyme) gene dataset derived from cow fecal microbiota. Thirteen of them exhibited β-glucosidase activity, with DCF-bgl-26 and DCF-bgl-27 showing relatively high expression levels and β-glucosidase activity. The maximum β-glucosidase activity of DCF-bgl-26 and DCF-bgl-27 was achieved at 45 °C and pH 6.0, with DCF-bgl-26 demonstrating better thermostability and pH tolerance compared to DCF-bgl-27. The activities of DCF-bgl-26 and DCF-bgl-27 were 123.2 U/mg protein and 157.9 U/mg protein, respectively, both of which are higher than those of many bacterial β-glucosidases. Structure analysis suggested that both β-glucosidases possess canonical (β/α)8-TIM barrel fold structure of GH1 family β-glucosidases. Thin-layer chromatography results showed that both enzymes could efficiently convert icariin to baohuoside I in 30 min, indicating they have potential application in the production of high value rare baohuoside I.
研究领域:
生物化学
·
生物技术
·
药理学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Xiangjiao Zheng,Zhiqiang Dong,Zepeng Liang,Yu Liu,Xiaowei Yin,Mofei Han,Zhongcheng Cui,Xifan Mei,Xiuqiu Gao
Introduction: Periodontitis is a chronic inflammatory disease brought on by various bacteria, and effective antibacterial, anti-inflammatory and alveolar bone regeneration are the main goals of treating periodontal disease. Methods: In the current work, we employed Icariin (ICA) into a hydrogel modified with carbon nanofiber (CNF) to create a multifunctional composite nanoplatform. The composite was activated in the near infrared (NIR) to treat periodontitis. Results: The antibacterial results showed that the ICA+CNF@H showed 94.2% and 91.7% clearance of S. aureus and E. coli , respectively, under NIR irradiation. In vitro experiments showed that NIR-irradiated composites suppressed inflammatory factor (IL-6) and ROS expression and up-regulated the performance of anti-inflammatory factor (IL-10) in RAW264.7 cells. At the same time, the composites promoted the production of osteogenic factors in BMSCs, with an approximately 3-fold increase in alkaline phosphatase activity after 7 days and an approximately 2-fold increase in the rate of extracellular matrix mineralization after 21 days. In vivo tests showed that the alveolar bone height was clearly greater in the ICA+CNF@H (NIR) group compared to the periodontitis group. Discussion: In conclusion, ICA+CNF@H under NIR irradiation achieved a synergistic effect of antibacterial, anti-inflammatory, reduction of reactive oxygen species and promotion of osteogenesis, offering a novel approach for treating periodontitis.
研究领域:
牙科
·
材料科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
CURRENT PHARMACEUTICAL DESIGN
·
2017
·Vol. 23
·期 39
·6071-6078
·
IF 2.611
Hua Wenbin,Zhang Yukun,Wu Xinghuo,Kang Liang,Tu Ji,Zhao Kangcheng,Li Shuai,Wang Kun,Song Yu,Luo Rongjin,Shao Zengwu,Yang Shuhua,Yang Cao
Background: Low back pain is a common problem, mainly caused by intervertebral disc degeneration (IDD). An important pathophysiological characteristic of IDD is the loss of homeostatic balance of the extracellular matrix metabolism. Interleukin-1β (IL-1β) is one of the inflammatory
mediators stimulating the degradation of extracellular matrix in the nucleus pulposus (NP) and contributing to IDD pathogenesis. Icariin, which is isolated from Epimedium brevicornum, acts as an anti-inflammatory drug. Objective: This study aimed to explore the pharmacological effects
of icariin in IDD by simulating NP inflammation in vitro. Method: Human NP cells were isolated and cultured in vitro. NP cells were pretreated with icariin (0.1, 1 and 10 μM) and stimulated by IL-1β (10 ng/ml). The concentration of Prostaglandin E2 was determined by enzymelinked
immunosorbent assay. Nitric oxide was determined by Griess reagent assay. The expression of cyclooxygenase- 2 (COX-2), inducible nitric oxide synthase (iNOS), degrading enzymes, collagen II, aggrecan, mitogenactivated protein kinase (MAPK), and nuclear factor-kappa B (NF-ΚB)-related signaling
molecules was assessed via western blotting. Results: IL-1β induced pronounced expression of COX-2 and iNOS, and stimulated production of prostaglandin E2 and nitric oxide. Icariin exhibited significant anti-inflammatory effect, inhibiting IL-1β-induced production of degrading
enzymes, as well as extracellular matrix reduction. Finally, icariin suppressed IL-1β-induced activation of MAPK- and NF-ΚB-related signaling pathways. Conclusion: The present findings suggest that icariin may have a protective effect on NP cells. The antiinflammatory effect
may contribute to the therapeutic action of icariin in IDD.
研究领域:
药理学
·
骨科
·
细胞生物学
·
炎症
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2026
·
IF 5.6
Wenjie Guo,Jinlun Feng,Yue Yang,Junying Chen,Tingting Ma,Mengyu Gao,Qiwei Li,Jianguo Liao,Yijia Guan,Yanru Zhang
The application of traditional gel dressings is limited by their poor sealing properties and inadequate anti-inflammatory effects. In this study, a temperature-sensitive hydrogel loaded with an anti-inflammatory agent was developed to address this issue. Initially, Icariin (ICA), which possesses both anti-inflammatory and antibacterial properties, was incorporated into nano-bioactive glass (NBG). Subsequently, the ICA-loaded NBG was incorporated into a sodium alginate (SA)/Pluronic F127 hydrogel (F127) system to prepare a thermosensitive composite hydrogel (FSB gel). The results demonstrated that the prepared FSB gel exhibits excellent thermosensitivity, injectability, and stability, as well as good biocompatibility. It accelerates the inflammatory response, promotes angiogenesis and collagen deposition, and enables scar-free healing of rat wounds within 14 days. Therefore, this study provides a viable option for the design of thermosensitive composite hydrogels intended to promote wound healing.
✓ 已验证
DOI
PubMed
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
·
2022
·
IF 6.633
Li-Ming Yu,Xue Dong,Yin-Li Xu,Zi-Jun Zhou,Yu-Ting Huang,Ji-Kai Zhao,Deng-Yue Xu,Xiao-Dong Xue,Qiu-Sheng Zhao,Tao Liu,Zong-Tao Yin,Hui Jiang,Hui-Shan Wang
Excessive alcohol consumption has long been identified as a risk factor for adverse atrial remodeling and atrial fibrillation (AF). Icariin is a principal active component from traditional Chinese medicine Herba Epimedii and has been demonstrated to exert potential antiarrhythmic effect. The present study was designed to evaluate the effect of icariin against alcohol-induced atrial remodeling and disruption of mitochondrial dynamics and furthermore, to elucidate the underlying mechanisms. Excessive alcohol-treated C57BL/6 J mice were infected with serotype 9 adeno-associated virus (AAV9) carrying mouse SIRT3 gene or negative control virus. Meanwhile, icariin (50 mg/kg/d) was administered to the animals in the presence or absence of AAV9 carrying SIRT3 shRNA. We noted that 8 weeks of icariin treatment effectively attenuated alcohol consumption-induced atrial structural and electrical remodeling as evidenced by reduced AF inducibility and reversed atrial electrical conduction pattern as well as atrial enlargement. Furthermore, icariin-treated group exhibited significantly enhanced atrial SIRT3-AMPK signaling, decreased atrial mitoSOX fluorescence and mitochondrial fission markers, elevated mitochondrial fusion markers (MFN1, MFN2) as well as NRF-1-Tfam-mediated mitochondrial biogenesis. Importantly, these beneficial effects were mimicked by SIRT3 overexpression while abolished by SIRT3 knockdown. These data revealed that targeting atrial SIRT3-AMPK signaling and preserving mitochondrial dynamics might serve as the novel therapeutic strategy against alcohol-induced AF genesis. Additionally, icariin ameliorated atrial remodeling and mitochondrial dysfunction by activating SIRT3-AMPK signaling, highlighting the use of icariin as a promising antiarrhythmic agent in this circumstance.
研究领域:
心脏病学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIVES OF VIROLOGY
·
2021
·Vol. 166
·期 9
·2443-2450
·
IF 2.574
Cui Zhanding,Wang Qian,Li Dengliang,Zhao Shihui,Zhang Qian,Tan Yuxin,Gong Qianwen,Liu Ting,Shao Jiang,Zhang Shuang,Huang Hailong,Wang Junzheng,Pei Zhihua,Dong Hao,Wang Kai,Hu Guixue,Li Zhiyong
Cats infected with feline calicivirus (FCV) often display oral ulcers and inflammation of the upper respiratory tract, which can lead to death in severe cases. Antiviral therapy is one of the most effective ways to control FCV infection. Natural compounds in Chinese herbal medicines and medicinal plants provide abundant resources for research on antiviral drugs. In this study, we found that icariin (ICA), formononetin (FMN) and caffeic acid phenethyl ester (CPAE) show low cytotoxicity towards F81 cells, that the three natural compounds have apparent antiviral effects on FCV in vitro , and that they can inhibit different FCV strains. Then, we found that ICA and FMN mainly function in the early stage of FCV infection, while CAPE can function in both the early and late stages of FCV infection. Finally, we found that ICA has an antagonistic effect on FMN and CAPE in FCV infection, and FMN has a synergistic effect with CAPE against FCV infection. Our results showed that ICA, FMN and CAPE may be potential drug candidates for FCV-induced diseases.
研究领域:
兽医学
·
药理学
·
病毒学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science and Technology
·
2023
·
IF 2.602
LI Gang,GE Xiaohong
Icariin has low bioavailability and poor stability, which limits its wide application. The complexation of icariin and whey protein is expected to solve this problem, but there is no research on their interaction mechanism. In view of this, the related mechanism was studied systematically by spectrofluorimetry and molecular docking method in this study. The fluorescence analysis showed that icariin and whey protein could form a non-covalent complex driven by hydrophobic force, which led to the fluorescence quenching of whey protein. In this process, the microenvironment around the tyrosine residue and tryptophan residue of whey protein changed. The molecular docking analysis confirmed the existence of hydrophobic interaction and hydrogen bonding in the complex, which well confirmed fluorescence results. The obtained results can promote the application of icariin in food.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CELL BIOCHEMISTRY AND FUNCTION
·
2024
·Vol. 42
·期 3
·e4005
·
IF 2.8
Nannan Ding,Shanyue Sun,Shuting Zhou,Zhimei Lv,Rong Wang
Tubulointerstitial fibrosis is an inevitable consequence of all progressive chronic kidney disease (CKD) and contributes to a substantial health burden worldwide. Icariin, an active flavonoid glycoside obtained from Epimedium species, exerts potential antifibrotic effect. The study aimed to explore the protective effects of icariin against tubulointerstitial fibrosis in unilateral ureteral obstruction (UUO)-induced CKD mice and TGF-β1-treated HK-2 cells, and furthermore, to elucidate the underlying mechanisms. The results demonstrated that icariin significantly improved renal function, alleviated tubular injuries, and reduced fibrotic lesions in UUO mice. Furthermore, icariin suppressed renal inflammation, reduced oxidative stress as evidenced by elevated superoxide dismutase activity and decreased malondialdehyde level. Additionally, TOMM20 immunofluorescence staining and transmission electron microscope revealed that mitochondrial mass and morphology of tubular epithelial cells in UUO mice was restored by icariin. In HK-2 cells treated with TGF-β1, icariin markedly decreased profibrotic proteins expression, inhibited inflammatory factors, and protected mitochondria along with preserving mitochondrial morphology, reducing reactive oxygen species (ROS) and mitochondrial ROS (mtROS) overproduction, and preserving membrane potential. Further investigations demonstrated that icariin could activate nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway both in vivo and in vitro, whereas inhibition of Nrf2 by ML385 counteracted the protective effects of icariin on TGF-β1-induced HK-2 cells. In conclusion, icariin protects against renal inflammation and tubulointerstitial fibrosis at least partly through Nrf2-mediated attenuation of mitochondrial dysfunction, which suggests that icariin could be developed as a promising therapeutic candidate for the treatment of CKD. Significance statement Tubulointerstitial fibrosis is an unavoidable consequence of advancing chronic kidney disease (CKD) and imposes a substantial global health burden. However, few effective therapeutic agents are available at present. The present study investigated the ameliorative effects of icariin on tubulointerstitial fibrosis (TIF) both in vivo and in vitro, and demonstrated that icariin inhibited inflammation and oxidative stress, at least partially, through improving Nrf2 activity and subsequent mitochondrial function, and eventually mitigated TIF and preserved renal function, suggesting that icariin could be developed as a promising therapeutic candidate for the treatment of CKD.
研究领域:
药理学
·
肾脏生理学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Jingfeng Sun,Jinying Li,Peining She,Yang Xiao,Tao Zhang,Guorui Li,Jiaming Ye,Zhanlun Ou,Jianghao Bi,Shaochuan Li
Bioactivity is an important factor to be considered for orthopedic implants as it determines the success of the implant. Polyetheretherketone (PEEK), an orthopedic biomaterial, has been widely used in biomedical engineering. However, unsatisfactory bioactivity continues to plague the clinical application of PEEK. In this study, icariin (Ica) coating was prepared on carbon fiber-reinforced PEEK (CF/PEEK) surfaces by constructing an assistant platform of polydopamine (PDA) aimed at enhancing the bioactivity and osteogenic capacity. The physicochemical properties of the fabricated CF/PEEK were further explored, along with its morphology, release, wettability and mechanical properties. The CF/PEEK surface with an Ica coating accelerated the proliferation of BMSC cells obviously and exhibited good biocompatibility, suggesting that it has favorable biosafety in vitro. In addition, the osteogenic properties of the Ica coating were demonstrated by the upregulated expression of osteogenesis-associated genes, alkaline phosphatase and mineralization in vitro. Furthermore, in vivo, the CF/PEEK implants coated with the Ica coating promoted the osseointegration at the implant–bone interface compared with the CF/PEEK without Ica modification, and there was no significant inflammatory response for each implantation group. Our research provides a promising modification strategy for loading Ica on the PDA-assistant platform-modified CF/PEEK implants with cytocompatibility, non-immunogenicity, and osteogenic properties, which broaden the clinical modification application of TCM monomers for orthopedic implants.
研究领域:
生物材料学
·
骨科学
·
表面工程
·
组织工程
·
转化医学
·
中药药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2020
·
IF 3.263
Yanzheng Xie,Lei Xie,Ailan Chen,Shengnan Wu,Yuxiao Mo,Xiaoqiang Guo,Changchun Zeng,Xinan Huang,Jinyang He
AIDS , a serious fatal disease caused by the human immunodeficiency virus (HIV), is an epidemic disease for which no effective vaccine has been established. The current therapeutic interventions for AIDS have limited efficacy because they are unable to clear HIV infections and the continuous occurrence of resistant HIV strains. Therefore, the exploitation of new drugs to prevent the spread of AIDS remains a high priority. In this study, the effects of icariin and its metabolite anhydroicaritin on SIV/HIV replication were investigated. In CEM × 174 cells and PBMC cells, both icariin and anhydroicaritin can significantly inhibit HIV-1 or SIVmac251 replication. Furthermore, molecular docking studies revealed that icariin and anhydroicaritin can act on both HIV reverse transcriptase and protease but could not bind to integrase . Reverse transcriptase and protease inhibition biological assays showed that both icariin and anhydroicaritin could significantly inhibit only HIV reverse transcriptase. In summary, the two compounds can significantly inhibit HIV/SIV in vitro and their targets may be mainly involved with HIV reverse transcriptase.
研究领域:
病毒学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EXPERIMENTAL CELL RESEARCH
·
2022
·
IF 4.145
Jia-Wen Teng,Si-Shan Bian,Peng Kong,Yun-Gang Chen
Background The physical health and quality of life of the elderly are severely affected by osteoporosis (OP). Methods We explored the regulatory mechanism of ICA in vivo and in vitro by constructing OP rats and inducing osteogenic differentiation of BMSCs. First, we determined the expression of miR-335–5p in bone tissues of OP patients, bone tissues of OP rats, and osteogenic BMSCs by RT-qPCR. Alizarin red staining was employed to detect the formation of calcium nodules in the cells. MTT was used to detect cell viability . Finally, we detected the bone tissue changes in OP rats by overexpression of miR-335–5p or oral ICA. Results miR-335–5p was lowly expressed in bone tissues of OP patients and OP rats. ICA treatment reversed the inhibitory effect of miR-335–5p inhibitor on BMSCs matrix mineralization . Moreover, PTEN was verified to be a downstream effector of miR-335–5p. During ICA induction, overexpression of PTEN reversed the promotive effect of miR-335–5p mimics on the osteogenic differentiation of BMSCs. In vivo experiments also found that overexpression of miR-335–5p or ICA treatment improved the pathogenesis of OP in rats. Conclusion ICA improved OP by up-regulating miR-335–5p to inhibit PTEN, thereby providing a new strategy for the prevention and treatment of OP.
研究领域:
药理学
·
分子生物学
·
干细胞研究
·
骨骼疾病
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）