INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shiwen Zhao,Danyang Wang,Qiong Zhou,Beibei Wang,Zhao Tong,Honglei Tian,Jianke Li,Yuhuan Zhang
Nanozymes with oxidase or peroxidase-mimicking activity have emerged as a promising alternative for disinfecting resistant pathogens. However, further research and clinical applications of nanozymes are hampered by their low in vivo biosafety and biocompatibility. In this study, inulin-confined gold nanoparticles (IN@AuNP) are synthesized as an antibacterial agent via a straightforward in situ reduction of Au 3+ ions by the hydroxyl groups in inulin. The IN@AuNP exhibits both peroxidase-mimicking and oxidase-mimicking catalytic activities, of which the maximum reaction velocity ( V max ) for H 2 O 2 is 2.66 times higher than that of horseradish peroxidase. IN@AuNP can catalyze the production of reactive oxygen species (ROS), resulting in effective antibacterial behavior against both Gram-negative ( Escherichia coli ) and Gram-positive ( Staphylococcus aureus ) bacteria. Abundant hydroxyl groups retained in inulin endow the nanozyme with high adhesion to bacteria, reducing the distance between the captured bacteria and ROS, achieving an antibacterial ratio of 100 % within 1 h. Importantly, due to the natural biosafety and non-absorption of the dietary fiber inulin, as well as the inability of inulin-trapped AuNP to diffuse, the IN@AuNP exhibits high biosafety and biocompatibility under physiological conditions. This work is expected to open a new avenue for nanozymes with great clinical application value.
研究领域:
生物医学工程
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材料科学
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微生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yuan Chen,Wenqiang Tan,Qing Li,Fang Dong,Guodong Gu,Zhanyong Guo
Inulin is a kind of renewable and biodegradable carbohydrate with good water solubility and numerous physiological functions. For further utilization of inulin, chemical modification can be applied to improve its bioactivities. In this paper, five novel inulin derivatives were synthesized via chemical modification with quaternary phosphonium salt. Their antifungal activity against three kinds of plant pathogens including Colletotrichum lagenarium , Phomopsis asparagi, and Fusarium oxysporum was assessed with radial growth assay in vitro . Results revealed that all the inulin derivatives exhibited improved antifungal activity compared with inulin. Particularly, inulin modified with triphenylphosphine (TPhPAIL) exhibited the best antifungal activity with inhibitory indices of 80.0%, 78.8%, and 87.4% against Colletotrichum lagenarium , Phomopsis asparagi , and Fusarium oxysporum at 1.0 mg/mL respectively. The results clearly showed that chemical modification of inulin with quaternary phosphonium salt could efficiently improve derivatives' antifungal activity. Further analysis of results indicated that the antifungal activity was influenced by alkyl chain length or electron-withdrawing ability of the grafted quaternary phosphonium salts. Longer alkyl chain lengths or the stronger electron-withdrawing groups would lead to enhanced antifungal efficacy.
研究领域:
植物学
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微生物学
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化学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2022
·
IF 11.504
Keying Han,Shanshan Li,Yuling Yang,Xiao Feng,Xiaozhi Tang,Yumin Chen
This paper studied the effects of inulin addition (0–20 mg mL −1 ) on the properties of chicken myofibrillar protein (MP) heat-induced gel. The molecular forces to maintain the gel structure, gelling formation process and gel microstructure were studied to explore the influencing mechanism of inulin on gel properties of MP. The results showed that the springiness and hardness of MP-inulin gel significantly increased when inulin was added at 5–20 mg mL −1 , while the water holding capacity (WHC) significantly enhanced at 5–10 mg mL −1 inulin addition ( P < 0.05). The most appropriate inulin addition to improve the properties of MP mixed gel was 10 mg mL −1 . The addition of 20 mg mL −1 inulin decreased the cooking loss of mixed gel. Inulin promoted the exposure of more hydrophobic groups and the formation of disulfide bonds. Inulin addition significantly decreased the I850/I830 in Raman spectrometry , indicating an increase in the formation of hydrogen bonds with MP molecules. However, inulin had little effect on electrostatic interaction between MP molecules. During the heating process, the highest storage modulus (G′) value was observed in MP samples added with 15 mg·mL −1 inulin at gel forming and strengthening stage. The denaturation enthalpy (ΔH) value of myosin decreased when inulin exceeded 10 mg mL −1 . The microstructure showed that MP composite gel with 10 mg mL −1 inulin had a dense and well-aggregated network structure. These results indicated that inulin addition could make MP denaturation easier during heating, strengthen the molecular forces of MP and the gel network structure, thus improving the MP gel properties.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Sheng Wang,Pingping Yuan,Shijie Zhou,Lin Li,Wenjing Peng,Shaoqi Qu
Current antibiotic-based treatments for bacterial enteric infections often disrupt gut microbiota and face reduced efficacy due to gastrointestinal complexity and mucosal barriers. Here, we developed a genistein-loaded nanoparticle using whey protein isolate and prebiotic inulin (Gen_Wi) to improve the gut microbiome health. Gen_Wi nanoparticles mitigated oxidative stress and inflammation in intestinal epithelial cells by activating Nrf2 and suppressing NF-κB pathways, reducing TNF-α and IL-1β levels by nearly 39 and 55.6%, respectively. Remarkably, oral administration of Gen_Wi significantly mitigated Salmonella-induced intestinal damage, reducing bacterial colonization by 1000-fold, restoring goblet cell counts, and enhancing tight junction proteins. Mechanistic analysis revealed that Gen_Wi reshaped gut microbiota composition by promoting beneficial bacteria and increasing production of gut barrier-strengthening short-chain fatty acids, including acetate, propionate, and butyrate. These results highlight Gen_Wi as a prebiotic system that integrates prebiotic functionality with anti-inflammation to combat enteric infections through modulation of host–microbe interactions.
研究领域:
微生物学
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药剂学
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免疫学
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胃肠病学
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纳米技术
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营养科学
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产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2024
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IF 8.7
Xuan Zhang,Xia Gao,Xiangzhou Yi,Hui Yu,Mingyang Shao,Yongcheng Li,Xuanri Shen
The pathogenesis of ulcerative colitis (UC) is closely related to severe inflammation, damaged colonic mucosal barrier, increased oxidative stress and intestinal ecological imbalance. However, due to the nonspecific distribution and poor bioavailability of drugs, UC treatment is still a serious challenge. Here, a mitochondria/colon dual targeted nanoparticles based on redox response was developed to effectively alleviate UC. Cannabidiol nanoparticles (CBD NPs) with a particle size of 143.2 ± 3.11 nm were prepared by self-assembly using polymers (TPP–IN–LA) obtained by modifying inulin with (5-carboxypentyl) triphenyl phosphonium bromide (TPP) and α-lipoic acid (α-LA). Excitingly, the constructed CBD NPs showed excellent mitochondrial targeting, with a Pearson correlation coefficient of 0.76 at 12 h. The results of animal imaging in vivo showed that CBD NPs could be effectively accumulated in colon tissue. Not only that, CBD showed significant glutathione stimulated release in the presence of 10 mM glutathione at pH 7.4. The results of in vivo animal experiments showed that CBD NPs significantly ameliorated DSS-induced colonic inflammation by modulating the TLR4-NF-κB signaling pathway. Moreover, CBD NPs significantly improved the histological damage of colon in UC mice, increased the expression level of tight junction protein ZO-1, and effectively restored the intestinal mucosal barrier function and intestinal mucosal permeability. More importantly, CBD NPs significantly improved the species composition, abundance and amount of short chain fatty acids of intestinal flora in UC mice, thus effectively maintaining the balance of intestinal flora. The dual-targeted and glutathione-responsive nanoparticles prepared in this study provide a promising idea for achieving targeted delivery of CBD for effective treatment of UC.
研究领域:
药学
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纳米技术
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炎症性肠病
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药物递送系统
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结直肠疾病
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 11
·期 12
·10386-10396
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IF 4.171
Huali Wang,Guijie Chen,Xiang Li,Fuping Zheng,Xiaoxiong Zeng
β-Glucan, an economical by-product of yeast, has various health-promoting activities. However, the digestion and fermentation characteristics of β-glucan from yeast are still unknown. Thus, simulated digestion under saliva, gastric and small intestinal solutions and fermentation by gut microbiota were studied in this work. The results showed β-glucan could not be hydrolyzed in saliva, gastric and small intestinal conditions. Then, β-glucan reaches the large intestine, where it is degraded and metabolized by gut microbiota. At the same time, β-glucan could modulate the structure and composition of gut microbiota by inhibiting the proliferation of harmful gut microbiota and promoting the growth of health-promoting gut microbiota. At the phylum level, β-glucan significantly decreased the ratio of Firmicutes to Bacteroidetes. Furthermore, both β-glucan and inulin could selectively promote the growth of Bifidobacterium. Unlike inulin, β-glucan was able to better promote the growth of Bifidobacterium_longum. Thus, β-glucan showed a similar probiotic activity to inulin and is expected to be a potential prebiotic for the modulation of gut microbiota.
研究领域:
营养学
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微生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 18
·3194
·
IF 5.1
Henan Sun,Hao Yu,Huiqiang Yu,Gaowa Saren,Ke Feng,Wenzhong Hu
A water-soluble, low-molecular-weight polysaccharide fraction, designated OJP-2, was extracted from the roots ofOphiopogon japonicus; its structural characteristics and immunomodulatory activity in macrophages were subsequently investigated. OJP-2 exhibited a narrow apparent molecular weight distribution with an average molecular weight (Mw) of 3568 Da and was primarily composed of fructose (0.784) and glucose (0.208). Structural analysis—integrating UV spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, and 1D/2D nuclear magnetic resonance (NMR) spectroscopy—revealed that OJP-2 is an inulin-type fructan. Its structure is characterized predominantly by β-(2→1)-linked Fruf chains and terminal α-D-Glcp residues, with potential signals indicating C-6-substituted Fruf units. In vitro immunological studies demonstrated that OJP-2 promoted nitric oxide (NO) production and enhanced the secretion of IL-6, IL-1β, and TNF-α in RAW264.7 macrophages. Under LPS/IFN-γ stimulation, OJP-2 induced non-monotonic changes in the CD86/CD206 macrophage phenotype, with the most pronounced effects observed at a concentration of 50 μg/mL. Furthermore, Western blot analysis showed that, compared to the Model group, treatment with OJP-2 resulted in a downward trend in the relative levels of p-p65/p65 and p-IκBα/IκBα across the tested concentration range. Collectively, these findings indicate that OJP-2 modulates macrophage activation phenotypes and influences NF-κB-related signaling pathways. Thus, OJP-2 is a candidate fructan for further investigation of immunomodulatory activity.
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DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2026
·
IF 15
Qiangyuan Fan,Chen Kan,Kesheng Wang,Huanhuan Zhu,Yue Zhang,Meiling Lu,Shaobo Xue,Lin Han,Zhongmin Geng,Weiliang Hou,Zunzhen Ming
Scavenging excess reactive oxygen species (ROS) and positively improving intestinal dysbiosis is a promising therapeutic strategy for alleviating inflammatory bowel disease (IBD). However, conventional clinical drugs and probiotic-based adjuvant therapies often fail to achieve satisfactory results due to systemic side effects of drugs, low bioactivity of probiotics, short intestinal retention time, and poor targeting ability. To address these challenges, we developed an antioxidant-functionalized curcumin loaded nanocomposite inulin hydrogel for targeted IBD therapy. In this system, curcumin was encapsulated within chitosan-coated Poly (lactic-co-glycolic acid) PLGA nanoparticles, which not only exhibited excellent ROS-scavenging capacity but also demonstrated the enhanced cellular uptake behavior. In a dextran sulfate sodium induced ulcerative colitis mouse model, the nanocomposite hydrogel significantly prolonged the intestinal retention time of curcumin, thereby suppressing the expression of pro-inflammatory factors, alleviating intestinal inflammation, and promoting the recovery of intestinal barrier and microbial diversity. This study has developed a synergistic therapeutic strategy via combining anti-inflammatory effects with gut microbiota regulation, offering a novel therapeutic approach for the clinical management of colitis.
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Runqi Zhu,Wenhui Yuan,Anqi Xia,Xujie Sun,Wenlu Yan,Ting Wu,Guanru Wang,Yu Li,Qi Yin,Yaping Li
In consideration of the important influence of gut microbiota on colorectal cancer (CRC) development and host immune system, regulating microbiota and intestinal environment can be an assisting approach to CRC treatment. To remodel tumor microenvironment, the anti-CRC molecular targeting drug regorafenib (REG)-encapsulating nanoparticle (UIRN) is constructed with the amphiphilic inulin-ursodeoxycholic acid conjugate. UIRN increases the intra-tumoral concentration and accumulation time of REG. In the CT26 tumor-bearing mouse model, the relative abundance of probiotics and short-chain fatty acid concentrations in colon are upregulated after treatment with UIRN, with decreased proportion of pro-tumoral bacteria. Combining gut microbiota modulation and the function of REG to induce polarization of tumor-associated macrophages toward the M1-type, UIRN conquers immunosuppression and enhances anti-tumor immunity. UIRN not only efficiently controls tumor progression alone but also exhibits adjuvant effects to chemotherapy and immunotherapy agents. The microbiota-regulating and tumor-suppressing functions of UIRN also work in the colitis-associated CRC model. With good biosafety, UIRN displays hopeful application prospects in CRC therapy.
研究领域:
肿瘤学
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药理学
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微生物学
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免疫学
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DOI
高影响因子
Nano Today
·
2024
·
IF 13.2
Dan Lu,Qi Wu,Xuanshou Xu,Qi Lyu,Chang Liu,Haixia Yuan,Minfeng Huo,Huixiong Xu,Liying Wang
Acute colitis is characterized by the microenvironment with a high level of inflammation, oxidative stress as well as a disordered microbiome . Sustained delivery of the therapeutic medicine into the colitis foci is limited due to the harsh condition of the gastric environment and fast excretion kinetics in the gut. Herein, we have designed an orally administrable probiotic inulin gel (igel) containing living Bacillus subtilis ( B. subt ) engineered with outperformed antioxidative single manganese atomic nanocatalyst (SAM NCs) for effective, sustained, and biocompatible therapeutics against acute colitis. Based on the multi-catalytic antioxidative activities (superoxide dismutase, glutathione peroxidase, and catalase) of the synthetic SAM NCs, these nanocatalysts are capable of eliminating the oxidative stresses both in vitro and in vivo . With further engineering onto the living Bacillus subtilis and subsequent in situ gelation inside the inulin, the as-formed probiotic igel(SAM NCs@ B. subt ) could effectively target and colonize inside the colon region, triggering the in vivo anti-inflammatory, anti-oxidative and gut microbiome modulation performance with high biocompatibility. The present study provides a promising probiotic gelation therapeutic strategy against acute colitis, shedding light on the exploration and clinical transformation of gastrointestinal disease.
研究领域:
胃肠病学
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纳米技术
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药剂学
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微生物学
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生物医学工程
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产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Ruixue Zhang,Weiyao Yang,Hongda Zhang,Le Sang,Zhiping Zhao
This work presents the efficient, safe, and continuous production of 5-hydroxymethylfurfural (HMF) and levulinic acid (LA) from inulin in micropacked bed reactors (μPBRs) over HND-580. Through optimization reaction conditions, the highly selective production of fructose and LA from aqueous inulin and HMF from an inulin DMSO-water mixed solution can be achieved with respect to other reactor technologies. The highest yields of 89% for fructose, 81% for HMF, and 84% for LA can be obtained within a reaction time of 7.26 min. Polysaccharide inulin is used as the reaction substrate, and the space-time yield (STY) of HMF and LA in μPBRs is at least an order higher than that in conventional batch reactors. Subsequently, an intrinsic kinetic model for inulin conversion is established. The most predicted values show good agreement with the experimental results with a relative error of ±20%.
研究领域:
化学工程
·
催化
·
生化工程
·
可再生能源
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiahao Chen,Yun Gu,Zhen Chen,Yafei Tan
In this study, the efficacy of a whey protein isolate-inulin (WPI-INU) complex emulsion in increasing the bioaccessibility of quercetin was evaluated from the aspects of structural stability, storage stability, oxidative stability, and its in vitro digestion characteristics. The quercetin encapsulation efficiency of the WPI-INU complex emulsion was 83.62 ± 0.73%, and the water solubility of quercetin encapsulated in the complex showed a 389-fold increase, which was 11.45% greater than that of quercetin encapsulated the whey protein isolate emulsion (W-QE). Free radical scavenging experiments and accelerated oxidation experiments confirmed that WI-QE can quickly eliminate free radicals. The presence of inulin increased the viscoelasticity of the WI-QE complex emulsion and prevented oil droplet release and aggregation. The digestibility of WI-QE was 8.59% greater than that of W-QE, and the bioaccessibility of WI-QE increased nearly 6-fold. In conclusion, the WPI-INU complex emulsion is a promising system for quercetin transportation, with implications for the use of this complex emulsion in the food and beverage industry.
研究领域:
食品科学
·
营养学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）